ISfinder Mirror Site

ISDge18

Family IS200/IS605
Group IS1341

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_008025	ND	Deinococcus geothermalis	Deinococcus geothermalis DSM 11300 Deinococcus geothermalis DSM 11300 plasmid I

DNA section

IS Length : 1388 bp

Ends

Left end : AGGGACGCTCCGAGGATGCCCCGGTTTTCAAGCCGGGGAGGAATCGGTGCGCCACCGTAGGTGGCGATGCCCTTTGCTCTGCTGAAGCCTTGGGCTATCC II struct. : Yes
Right end : TGGAGTAAAGGTGCGCGAGGCGCAAGTTTCGCACACAGACTCCAACCTCGTAAGCCCCGCGTAGGGGAACCCCCGGTCTTCAGGCCGGGGAGGATGTCAG II struct. : Yes

Insertion site

Left flank	LE cleavage site	Right flank	RE cleavage site
CATCGCCCCC	TCAC	TGCCCGCCATGCAGGC	tcag

DNA sequence

AGGGACGCTCCGAGGATGCCCCGGTTTTCAAGCCGGGGAGGAATCGGTGCGCCACCGTAGGTGGCGATGCCCTTTGCTCTGCTGAAGCCTTGGGCTATCC
TATGTTCATGCACAGGGCGGAAACGGTCATTCTCAAACTGGCCGCCCCGTCTGCGAACAAACGAGCGTGGTTGTCTCAGACCGCTCAGGCGTTCCGGGAC
GGGGTAGGGATGGTTTTGGGTGTGGCTGAATCCGAGAAGACGGGCAGTCGGGCCAAACTGCACAACGCCTGCTACGCCGCCATCCGGGAGGAAACTGGCC
TGCCCAGCGACTACGCCCGCATGGCGGTCAACGCAGGGGTTGCGCTGGCCCGTTCCTACTGGGCCTTGCGAAAGGGAAAGCGCCGCCCCTCCTTTCCGAA
GGTGGGGAAGTCTCAGGGCATTGGGCTGGGCGTCAATGCCTACGCTGTCCTGAAACGCGGTGAGCGGTTCTTTCTGCGGGCCTCTACTGGGAAGCGTGGC
TCCTACAAGTGGTTTCCACTTTCCGTACCTGTGCATCTGGCCTCCAAACTTGAGTTCGTTCATGGCGACGCCAAGCTGTTCCAGCGTGGAAGAGATTGGT
TCGTCATGCTTCCGCTTCGCCTCCCCCACACGCCAGCCGTCCGTGACGGTGAACCTACGGTTCTGGGCGTGGATTTGGGCGTGGTGAAGCTGATGACGGT
GCTGACACCGGACGGCGTGAAGATTTGGAACGGTCTGCCTGTGCGCCATCGCCGGGAGAGATTCGCTGCTGTTCGTCGCCGCCTCCAGAGGCATCGCCGC
ACCGACCGCGTGAAGGCGCAGGGAGGGCGTGAACGCCGCTGGATGTCGGGGCTGAACCACAAACTCAGTCGTGAACTCGTTGACCTTGCCCGCTTCTACA
AGGACGCGGTCATCGCCTTTGAGCAGCTGGACGGGATTCGTGACCGGGTACGGGGCAGCAAGAAGTTCAACCGGATGATGAGCAGCTGGACGTTCCGTGA
CCTCGTGGACAAGGTGCGGTACAAGGCGGAAGCGGCTGGGGTGGGCGTGGTCTTCGTTGACCCGCGCAAGACTTCCCAGACCTGCCACCGCTGCGGACAT
GCCACGCGGAGTAACCGGACGTCTCAGGCTGAGTTTCGATGTGTGAACTGCGGATTCAGGACGAATGCGGACTGGAACGCGGCCACCAACATCGCTGCTG
CCGGGCTGCGTGCCTTGCAGCAGGGGCCGACTGACACGGCCCGCTCTGCTCACGCAGAGCAGGCGTCGTCCTCGTTGACGGCGCTCGATGGAGTAAAGGT
GCGCGAGGCGCAAGTTTCGCACACAGACTCCAACCTCGTAAGCCCCGCGTAGGGGAACCCCCGGTCTTCAGGCCGGGGAGGATGTCAG

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
1245 bp	414 aa	108	1352	+	No

ORF function : Accessory Gene
AG : TnpB

ORF sequence :

MHRAETVILKLAAPSANKRAWLSQTAQAFRDGVGMVLGVAESEKTGSRAKLHNACYAAIREETGLPSDYARMAVNAGVALARSYWALRKGKRRPSFPKVG
KSQGIGLGVNAYAVLKRGERFFLRASTGKRGSYKWFPLSVPVHLASKLEFVHGDAKLFQRGRDWFVMLPLRLPHTPAVRDGEPTVLGVDLGVVKLMTVLT
PDGVKIWNGLPVRHRRERFAAVRRRLQRHRRTDRVKAQGGRERRWMSGLNHKLSRELVDLARFYKDAVIAFEQLDGIRDRVRGSKKFNRMMSSWTFRDLV
DKVRYKAEAAGVGVVFVDPRKTSQTCHRCGHATRSNRTSQAEFRCVNCGFRTNADWNAATNIAAAGLRALQQGPTDTARSAHAEQASSSLTALDGVKVRE
AQVSHTDSNLVSPA

Blast result :

Comments

ISDge18 is 42% aa similar to ISBce3. This IS had only the TnpB.

References

1] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina del Rio,T., Hammon,N., Israni,S., Dalin,E., Tice,H., Pitluck,S., Brettin,T., Bruce,D., Han,C., Tapia,R., Saunders,E., Gilna,P., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Kim,E., Daly,M.J., Fredrickson,J.K., Makarova,K.S., Gaidamakova,E.K., Zhai,M. and Richardson,P. US DOE Joint Genome Institute. (2006) Direct submission GenBank.