ISfinder Mirror Site

ISDge17

Family IS630
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_008010	ND	Deinococcus geothermalis	Deinococcus geothermalis DSM 11300 plasmid 1

DNA section

IS Length : 1071 bp

Ends

IR Length : 26/35

IRL : TTATTGAGTCCGGCATGGGTTGGAGACGACAGGTACACTGAGGGCGTGCT
IRR : CTATTGACTCCGGCTTAGGTTTGGGACGCACGGTAGGGGCGGGCGAGGCG

Insertion site

Left flank	Direct repeat	Right flank	DR Length
CGCCTCGTCTA	TA	GATGCGTGATCA	2

DNA sequence

TTATTGAGTCCGGCATGGGTTGGAGACGACAGGTACACTGAGGGCGTGCTCCGCGTCTGGCGACCCTCGACCCTGACCCGCGACCAACTGGAGGAACGGC
GGCTCTATGCTCAGCAACTCCTAGCCACAGGCGAGATCAGTACCAAGGAAATCGCGGAAACGCTCGGCGTTTCCGAAAGTACCGTCCGAACCTGGAAGCA
GCGCCTCCGTGAGCATGGGAGCCTCAAGGCTACCCAAGCTCCGGGGCCTCCCCAGCGCCTGAGTCCAGAACAGCGTGCTCAGTTGGAGGAACTGCTCCGC
GAAGGACCTCTGGCCGCTGGCTACCCCGACTCGCGTTGGACTACCCCTCGCGTGCGGGCGATCATCGGAACGCACTTTGACGTGTGGTATCACGCCGATC
ACGTCAGAACAGTCCTTCATCAGCTCGGCTTCAGTCCTCAGAAGCCAGAACCGCGTGCCCTGGAACGCAATGAGCAGGCCATTCAGACTTGGGTCGAGCA
CACGCTCCCCGAGTTGGAAAAAAAAGGTCGAGCAGGGCGCGACCCTCGTCTTCCTCGATGAAAGTGGCTTCAGTCTGAAGCCCACCGTGACCCGAACCTG
GGCCCCTCGAGGACAAACACCGATCCTTCGGACGAAAGCAGCTTGGGACAAGCTCTCGACCATCGGGGCCATCACCACGAGCGGCCAGTTCCTGCAACAC
ACACACTCGGGAGCCATTCGAGGTGCTCAGGTGGTGGCCTTCTGCCAACATCTTCTCCGGCACGTGCAGGGTGAATTCGTTGTGCTGATGGATAACGCCC
GCATCCACAAAACGAAGGCCCTGAGGGCCTTCGTTGAGCAGCAACCGCGTCTCACCATCGAATATCTTCCACCTTACGCGCCTGACCTCAACCCCATTGA
GCGGGTGTGGGCCTACATCAAAGGACCAATCCTGGGCAACTTCTGCGCCAAGGACATCGGCGAATTGAAGGGGAGACTGAAGGCTGCCTGGCAACGTGTT
CGCTACGTTCAGCTCCCCCAGCGCCTCGCCCGCCCCTACCGTGCGTCCCAAACCTAAGCCGGAGTCAATAG

Recoding section

Recoding by frameshift
Frame
Type
Experimentally demonstrated

Stimulators :

Shine-Dalgarno sequence :
Secondary structure :

Recoding motif :

Protein section

ORF number : 3

ORF 1

Length		Begin	End	Strand	Fusion ORF
507 bp	168 aa	55	561	+	No

ORF function : Transposase
Description : First part of the transposase

ORF sequence :

VWRPSTLTRDQLEERRLYAQQLLATGEISTKEIAETLGVSESTVRTWKQRLREHGSLKATQAPGPPQRLSPEQRAQLEELLREGPLAAGYPDSRWTTPRV
RAIIGTHFDVWYHADHVRTVLHQLGFSPQKPEPRALERNEQAIQTWVEHTLPELEKKGRAGRDPRLPR

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
795 bp	264 aa	263	1057	+	No

ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

VQNSVLSWRNCSAKDLWPLATPTRVGLPLACGRSSERTLTCGITPITSEQSFISSASVLRSQNRVPWNAMSRPFRLGSSTRSPSWKKKVEQGATLVFLDE
SGFSLKPTVTRTWAPRGQTPILRTKAAWDKLSTIGAITTSGQFLQHTHSGAIRGAQVVAFCQHLLRHVQGEFVVLMDNARIHKTKALRAFVEQQPRLTIE
YLPPYAPDLNPIERVWAYIKGPILGNFCAKDIGELKGRLKAAWQRVRYVQLPQRLARPYRASQT

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
1003 bp	333 aa	55	1057	+	Yes

ORF function : Transposase
Chemistry : DDE

ORF sequence :

VWRPSTLTRDQLEERRLYAQQLLATGEISTKEIAETLGVSESTVRTWKQRLREHGSLKATQAPGPPQRLSPEQRAQLEELLREGPLAAGYPDSRWTTPRV
RAIIGTHFDVWYHADHVRTVLHQLGFSPQKPEPRALERNEQAIQTWVEHTLPELEKKVEQGATLVFLDESGFSLKPTVTRTWAPRGQTPILRTKAAWDKL
STIGAITTSGQFLQHTHSGAIRGAQVVAFCQHLLRHVQGEFVVLMDNARIHKTKALRAFVEQQPRLTIEYLPPYAPDLNPIERVWAYIKGPILGNFCAKD
IGELKGRLKAAWQRVRYVQLPQRLARPYRASQT

Blast result :

Comments

ISDge17 is 48% aa similar to IS885. The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.

References

1] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina del Rio,T., Hammon,N., Israni,S., Dalin,E., Tice,H., Pitluck,S., Brettin,T., Bruce,D., Han,C., Tapia,R., Saunders,E., Gilna,P., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Kim,E., Daly,M.J., Fredrickson,J.K., Makarova,K.S., Gaidamakova,E.K., Zhai,M. and Richardson,P. US DOE Joint Genome Institute. (2006) Direct submission GenBank.