ISPsy24

  • Family IS3
  • Group IS3
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_005773 ND Pseudomonas syringae
Pseudomonas syringae pv. phaseolicola 1448A
DNA section
IS Length : 1235 bp

Ends


IR Length : 25/33

IRL : TGTAGTGGTCTAATGAAACCGGACACCCATTTAGGCGAGAATGCTCGCCA
IRR : TGTAGTGGTCAACTGATCCCGGACACGACGTTAAGTCTTTTTTCGGACTG

Insertion site


Left flankDirect repeatRight flankDR Length
CTGTAGATCCTAACGACCCAAATG4
CTCCACAGAGAGGAACGAATCTG3

DNA sequence

TGTAGTGGTCTAATGAAACCGGACACCCATTTAGGCGAGAATGCTCGCCAGATAGAGGTGTCTGATGACCAAACAACGCCGTACCTTTTCCGCTGAATTC
AAACGCGAGGCCGCAGGCCTCGTGCTCGATCAAGGCTATAGCCACATTGAGGCCTCTCGCTCGCTGGGAGTTGTCGAGTCCGCACTGCGTCGCTGGGTTA
ACCAGCTTCAACAGGAGCGCAACGGTGTCACTCCACAGAGCAAAGCCCTGACGCCCGAGCAGCAGAAAATTCAGGAGCTGGAAGCCCGGATTGCCCGTCT
TGAGCGGGAGAAATCCATTTTAAAAAAGGCTACCGCGCTCTTGATGTCGGAAGAGCACGAGCGCATGCGCTGATTGATCAGCTCAGTGCCCGTGAATCGG
TTGAGTTGCTGTGCGAAGCCTTTGAAGTACCTCGCTCGTGCTACTACAACCATCGCCTTAGACGGCGCAATCCAGACGTTGAGCGAGTCCGGCTACGCAG
CCGGGTCAACGAGCTGTTTACACAAAGTCGAAGCGCTGCCGGCAGCCGCAGCATCGTGTTGATGATGCATGAAGATGGCGAGCAGATTGGCCGGTTCAAG
GTACGTAGCCTGATGCGAGAGCTGGGATTGATCAGTAAGCAACCGGGATCGCATGCTTATAGAAAAGCGACGGTGGAGCGACCCGATATTCCGAATATTT
TGAATCGAGCATTCGATGTTCCAGCGCCCAATCAGGTGTGGTGCGGCGATATCACTTACATCTGGGCACAAGGGAAATGGCATTACCTGGCTGTGGTTAT
GGATCTTTTCGCGCGCCGAGTGGTGGGCTGGGCGTTATCGAGCAAACCGGATACTGATCTGGTAATCAAGGCGCTGGATATGGCCTACGAACAACGCGGC
AAGCCGCAGGGCCTGCTGTTTCACTCGGACCAGGGCGCCCAGTATGGCAGCCGACAATTTCGCCAGCGGCTTTGGCGATACCGCATACGCCAGAGCATGA
GTCGTCGTGGCAACTGCTGGGATAATGCGCCGATGGAGCGGGTGTTCCGGAGTTTGAAAACTGAGTGGATACCGACCGTGGGCTACATGACGGCCCAAGA
GGCTCAGCGAGACATCAGTCATTATCTGATGCATCGCTACAACTGGATTCGGCCACACCAGTTCAATAACGGACTGGCGCCAGCTCAGTCCGAAAAAAGA
CTTAACGTCGTGTCCGGGATCAGTTGACCACTACA
Recoding section
  • Recoding by frameshift
  • Frame
  • Type
  • Experimentally demonstrated

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
309 bp102 aa65373+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MTKQRRTFSAEFKREAAGLVLDQGYSHIEASRSLGVVESALRRWVNQLQQERNGVTPQSKALTPEQQKIQELEARIARLEREKSILKKATALLMSEEHER
MR

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
924 bp307 aa3041227+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

AGEIHFKKGYRALDVGRARAHALIDQLSARESVELLCEAFEVPRSCYYNHRLRRRNPDVERVRLRSRVNELFTQSRSAAGSRSIVLMMHEDGEQIGRFKV
RSLMRELGLISKQPGSHAYRKATVERPDIPNILNRAFDVPAPNQVWCGDITYIWAQGKWHYLAVVMDLFARRVVGWALSSKPDTDLVIKALDMAYEQRGK
PQGLLFHSDQGAQYGSRQFRQRLWRYRIRQSMSRRGNCWDNAPMERVFRSLKTEWIPTVGYMTAQEAQRDISHYLMHRYNWIRPHQFNNGLAPAQSEKRL
NVVSGIS

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1163 bp387 aa651227+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MTKQRRTFSAEFKREAAGLVLDQGYSHIEASRSLGVVESALRRWVNQLQQERNGVTPQSKALTPEQQKIQELEARIARLEREKSILKKGYRALDVGRARA
HALIDQLSARESVELLCEAFEVPRSCYYNHRLRRRNPDVERVRLRSRVNELFTQSRSAAGSRSIVLMMHEDGEQIGRFKVRSLMRELGLISKQPGSHAYR
KATVERPDIPNILNRAFDVPAPNQVWCGDITYIWAQGKWHYLAVVMDLFARRVVGWALSSKPDTDLVIKALDMAYEQRGKPQGLLFHSDQGAQYGSRQFR
QRLWRYRIRQSMSRRGNCWDNAPMERVFRSLKTEWIPTVGYMTAQEAQRDISHYLMHRYNWIRPHQFNNGLAPAQSEKRLNVVSGIS

 

Blast result :
Comments
ISPsy24 is 89% aa similar to IS222.The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
Found in argK-tox gene cluster.
References
1] Joardar,V., Lindeberg,M., Jackson,R.W., Selengut,J., Dodson,R., Brinkac,L.M., Daugherty,S.C., Deboy,R., Durkin,A.S., Giglio,M.G., Madupu,R., Nelson,W.C., Rosovitz,M.J., Sullivan,S., Crabtree,J., Creasy,T., Davidsen,T., Haft,D.H., Zafar,N., Zhou,L., Halpin,R., Holley,T., Khouri,H., Feldblyum,T., White,O., Fraser,C.M., Chatterjee,A.K., Cartinhour,S., Schneider,D.J., Mansfield,J., Collmer,A. and Buell,C.R. (2005) J. Bacteriol. 187 (18), 6488-6498.