ISPa31
- Family IS3
- Group IS3
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| DQ315788 | ND | Pseudomonas aeruginosa | Pseudomonas aeruginosa Sul-type |
DNA section
IS Length : 1671 bp
Ends
IR Length : 18/25
IRL : CCCCCGGTGTCAGGATAGTTGTCGCCTGATCGGATTCACCCAAACTGAAC
IRR : CGGCGTTTTTCAAGATAGTTGTCGCGTCGACCGTGTTTTCAGGCCGCCTT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GACCCGTTTG | GCTA | AGTGCTTGTG | 4 |
DNA sequence
CCCCCGGTGTCAGGATAGTTGTCGCCTGATCGGATTCACCCAAACTGAACCCCGGGGGCGGAAAGAGCCATGCAATGCCGCGTTACTCTGCTGAACGAAA
AGCTGCCCTGCTGAAGAAGCTGCTGCCACCGATCAACCTGTCCGTGGCTGAGGTCGCCCGCCAGGAAGGCGTCTCCGAGATGACCCTGTATGCTTGGCGC
AAACAGGCCAAGGCTGAAGGAGCCCCCGTGCCAGGAGACAAGAAACTGCCCGATGACTGGTCGGCCGAGGCCAAGTTCGCCGTCGTGCTGGAAACCGCTG
CGCTGTCGGAAATCGAACTGAGCGAGTACTGCCGACGTAAGGGGCTCTACCCCGAGCAGGTGCAGACCTGGCGGCAGGCGTGCATCAGCGGCCAGCAGTC
GGCACAGGCGCAGCGACAGGCCGAGCGCGAGCAAGCCAAGGCAGACAAGAAACGCATCCGTGAGCTGGAGCGCGAGCTGCGCCGCAAGGACAAGGCGTTG
GCCGAGGCGGCAGCGATTCTGGTGCTGCGAAAAAAGCTGAACGCCTACTGGGGCAACGACAGCGAGGACAACTGATCCCGCTGCCAGAACGGCAGAAACT
CGTTGAATGGCTGAACGAAGCCGTCGCCGCAGGTGCCCGTAAAACGTTGGCCTGCAAGGAGCTGAGCGTGTCCCTGCGCACCCTGCAACGCTGGACCGAG
GAGAACGTCGTGCGCGCTGATGCCCGCCCCACAGCCAAGCGACCGGTGCCGCGCAATGCACTGAGCCCGGCTGAGCGCGAAGCCATCCTGGCCGTCTGCA
ACAGCCCGGAATACGCGCATCTGCCGCCCAGCCAGATCGTGCCGAGGCTGGCGGATGCCGGGCGTTACCTGGCCTCGGAGTCGACGTTCTACCGCGTCCT
GCACGCAGCCGATCAGCAGCATCGCCGCGGTCGCAGCCAGCCACCGCGCACGGTGAAAGCGCCGACCAGCCATGTAGCGACCGCCGCCAATCAGGTCTGG
TCATGGGACATCACCTATCTCCCGGCGGCGGTACGCGGGAAGTATTTCTACCTGTACCTGATCGAGGACATCTACAGCCGCAAGGCGGTGGGCTGGGAGG
TCCACGAACAGGAAAGCGGCGAGCTGGGTGCCATGCTGCTGCAACGCAGCGTGCTCAGCGAGCAGTGCCTGCTCAAGCCGCCGGTACTGCACTCGGACAA
CGGTGCACCGATGAAATCGGCGACCATGCTGCAAAAGATGTACGACCTGGGCGTCACGCCGTCGCACAACCGCCCGCGGGTGAGCGACGACAACCCCTAC
TCGGAATCGCTGTTCCGCACGCTGAAATACTGCCCGCAGTGGCCGAAACAAGGCTTCGCCGACCTGGAAGCGGCACGTTGCTGGGTGAGAGATTTCATCC
GCTGGTACAACCACGAGCACCGGCACAGCCGCATCGGTTTCGTGACACCGGCAGAGCGCCATCGGGGTGAAGATCACCGCGCGCTGGCCCATCGCCATGC
GCTGTACCAAGAAGCCCGCCAGCGCATGCCGGAGCGCTGGTCAGGCACCACACGCAACTGGACGCCGGTCGGAGCGGTGACACTCAACCCCGAGCGGGAG
CAGGAACAACAGCCGCAGAAAAAGGCGGCCTGAAAACACGGTCGACGCGACAACTATCTTGAAAAACGCCG
AGCTGCCCTGCTGAAGAAGCTGCTGCCACCGATCAACCTGTCCGTGGCTGAGGTCGCCCGCCAGGAAGGCGTCTCCGAGATGACCCTGTATGCTTGGCGC
AAACAGGCCAAGGCTGAAGGAGCCCCCGTGCCAGGAGACAAGAAACTGCCCGATGACTGGTCGGCCGAGGCCAAGTTCGCCGTCGTGCTGGAAACCGCTG
CGCTGTCGGAAATCGAACTGAGCGAGTACTGCCGACGTAAGGGGCTCTACCCCGAGCAGGTGCAGACCTGGCGGCAGGCGTGCATCAGCGGCCAGCAGTC
GGCACAGGCGCAGCGACAGGCCGAGCGCGAGCAAGCCAAGGCAGACAAGAAACGCATCCGTGAGCTGGAGCGCGAGCTGCGCCGCAAGGACAAGGCGTTG
GCCGAGGCGGCAGCGATTCTGGTGCTGCGAAAAAAGCTGAACGCCTACTGGGGCAACGACAGCGAGGACAACTGATCCCGCTGCCAGAACGGCAGAAACT
CGTTGAATGGCTGAACGAAGCCGTCGCCGCAGGTGCCCGTAAAACGTTGGCCTGCAAGGAGCTGAGCGTGTCCCTGCGCACCCTGCAACGCTGGACCGAG
GAGAACGTCGTGCGCGCTGATGCCCGCCCCACAGCCAAGCGACCGGTGCCGCGCAATGCACTGAGCCCGGCTGAGCGCGAAGCCATCCTGGCCGTCTGCA
ACAGCCCGGAATACGCGCATCTGCCGCCCAGCCAGATCGTGCCGAGGCTGGCGGATGCCGGGCGTTACCTGGCCTCGGAGTCGACGTTCTACCGCGTCCT
GCACGCAGCCGATCAGCAGCATCGCCGCGGTCGCAGCCAGCCACCGCGCACGGTGAAAGCGCCGACCAGCCATGTAGCGACCGCCGCCAATCAGGTCTGG
TCATGGGACATCACCTATCTCCCGGCGGCGGTACGCGGGAAGTATTTCTACCTGTACCTGATCGAGGACATCTACAGCCGCAAGGCGGTGGGCTGGGAGG
TCCACGAACAGGAAAGCGGCGAGCTGGGTGCCATGCTGCTGCAACGCAGCGTGCTCAGCGAGCAGTGCCTGCTCAAGCCGCCGGTACTGCACTCGGACAA
CGGTGCACCGATGAAATCGGCGACCATGCTGCAAAAGATGTACGACCTGGGCGTCACGCCGTCGCACAACCGCCCGCGGGTGAGCGACGACAACCCCTAC
TCGGAATCGCTGTTCCGCACGCTGAAATACTGCCCGCAGTGGCCGAAACAAGGCTTCGCCGACCTGGAAGCGGCACGTTGCTGGGTGAGAGATTTCATCC
GCTGGTACAACCACGAGCACCGGCACAGCCGCATCGGTTTCGTGACACCGGCAGAGCGCCATCGGGGTGAAGATCACCGCGCGCTGGCCCATCGCCATGC
GCTGTACCAAGAAGCCCGCCAGCGCATGCCGGAGCGCTGGTCAGGCACCACACGCAACTGGACGCCGGTCGGAGCGGTGACACTCAACCCCGAGCGGGAG
CAGGAACAACAGCCGCAGAAAAAGGCGGCCTGAAAACACGGTCGACGCGACAACTATCTTGAAAAACGCCG
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 501 bp | 166 aa | 75 | 575 | + | No |
Description : First part of the transposase
ORF sequence :
MPRYSAERKAALLKKLLPPINLSVAEVARQEGVSEMTLYAWRKQAKAEGAPVPGDKKLPDDWSAEAKFAVVLETAALSEIELSEYCRRKGLYPEQVQTWR
QACISGQQSAQAQRQAEREQAKADKKRIRELERELRRKDKALAEAAAILVLRKKLNAYWGNDSEDN
QACISGQQSAQAQRQAEREQAKADKKRIRELERELRRKDKALAEAAAILVLRKKLNAYWGNDSEDN
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1170 bp | 389 aa | 464 | 1633 | + | No |
Description : Second part of the transposase
ORF sequence :
AGARAAPQGQGVGRGGSDSGAAKKAERLLGQRQRGQLIPLPERQKLVEWLNEAVAAGARKTLACKELSVSLRTLQRWTEENVVRADARPTAKRPVPRNAL
SPAEREAILAVCNSPEYAHLPPSQIVPRLADAGRYLASESTFYRVLHAADQQHRRGRSQPPRTVKAPTSHVATAANQVWSWDITYLPAAVRGKYFYLYLI
EDIYSRKAVGWEVHEQESGELGAMLLQRSVLSEQCLLKPPVLHSDNGAPMKSATMLQKMYDLGVTPSHNRPRVSDDNPYSESLFRTLKYCPQWPKQGFAD
LEAARCWVRDFIRWYNHEHRHSRIGFVTPAERHRGEDHRALAHRHALYQEARQRMPERWSGTTRNWTPVGAVTLNPEREQEQQPQKKAA
SPAEREAILAVCNSPEYAHLPPSQIVPRLADAGRYLASESTFYRVLHAADQQHRRGRSQPPRTVKAPTSHVATAANQVWSWDITYLPAAVRGKYFYLYLI
EDIYSRKAVGWEVHEQESGELGAMLLQRSVLSEQCLLKPPVLHSDNGAPMKSATMLQKMYDLGVTPSHNRPRVSDDNPYSESLFRTLKYCPQWPKQGFAD
LEAARCWVRDFIRWYNHEHRHSRIGFVTPAERHRGEDHRALAHRHALYQEARQRMPERWSGTTRNWTPVGAVTLNPEREQEQQPQKKAA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1559 bp | 519 aa | 75 | 1633 | + | Yes |
Chemistry : DDE
ORF sequence :
MPRYSAERKAALLKKLLPPINLSVAEVARQEGVSEMTLYAWRKQAKAEGAPVPGDKKLPDDWSAEAKFAVVLETAALSEIELSEYCRRKGLYPEQVQTWR
QACISGQQSAQAQRQAEREQAKADKKRIRELERELRRKDKALAEAAAILVLRKKAERLLGQRQRGQLIPLPERQKLVEWLNEAVAAGARKTLACKELSVS
LRTLQRWTEENVVRADARPTAKRPVPRNALSPAEREAILAVCNSPEYAHLPPSQIVPRLADAGRYLASESTFYRVLHAADQQHRRGRSQPPRTVKAPTSH
VATAANQVWSWDITYLPAAVRGKYFYLYLIEDIYSRKAVGWEVHEQESGELGAMLLQRSVLSEQCLLKPPVLHSDNGAPMKSATMLQKMYDLGVTPSHNR
PRVSDDNPYSESLFRTLKYCPQWPKQGFADLEAARCWVRDFIRWYNHEHRHSRIGFVTPAERHRGEDHRALAHRHALYQEARQRMPERWSGTTRNWTPVG
AVTLNPEREQEQQPQKKAA
QACISGQQSAQAQRQAEREQAKADKKRIRELERELRRKDKALAEAAAILVLRKKAERLLGQRQRGQLIPLPERQKLVEWLNEAVAAGARKTLACKELSVS
LRTLQRWTEENVVRADARPTAKRPVPRNALSPAEREAILAVCNSPEYAHLPPSQIVPRLADAGRYLASESTFYRVLHAADQQHRRGRSQPPRTVKAPTSH
VATAANQVWSWDITYLPAAVRGKYFYLYLIEDIYSRKAVGWEVHEQESGELGAMLLQRSVLSEQCLLKPPVLHSDNGAPMKSATMLQKMYDLGVTPSHNR
PRVSDDNPYSESLFRTLKYCPQWPKQGFADLEAARCWVRDFIRWYNHEHRHSRIGFVTPAERHRGEDHRALAHRHALYQEARQRMPERWSGTTRNWTPVG
AVTLNPEREQEQQPQKKAA
Blast result :
Comments
ISPa31 is 83% aa similar to IS1240.
File updated: May 03 2013. Extremities were modified, the proposed ends for this sequence are based on the similarities with closely relative elements.
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible -1 frameshift.
File updated: May 03 2013. Extremities were modified, the proposed ends for this sequence are based on the similarities with closely relative elements.
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible -1 frameshift.
References
1] Naas,T., Poirel,L. and Nordmann,P. (1999) Biochim. Biophys. Acta 1489 (2-3), 445-451.
2] Naas,T., Aubert,D., Lambert,T. and Nordmann,P. (2006) Antimicrob. Agents Chemother. 50 (5), 1745-1752.
2] Naas,T., Aubert,D., Lambert,T. and Nordmann,P. (2006) Antimicrob. Agents Chemother. 50 (5), 1745-1752.