ISLxc3
- Family IS30
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| EF421582 | ND | Leifsonia xyli | Leifsonia xyli subsp. cynodontis |
DNA section
IS Length : 1511 bp
Ends
IR Length : 21/27
IRL : CTTGGGTTCTAACGGTCGTTGCAACACCTGGACTTTCTGGGAGTTGTAAC
IRR : GGCGGATTCTAGTGGTCGTTGCAACACGACGATTAGCTGGTAGGTAGTTT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GGGCTGATCC | ATCTGGACGA | 0 |
DNA sequence
CTTGGGTTCTAACGGTCGTTGCAACACCTGGACTTTCTGGGAGTTGTAACGACCGTGGCCCGTTTAAGGAGCTATTCGAGGGATCGACGCTGTTTCACGC
AGGCTGATAAGGACGCGTTCTTCGCGTTGTTCGAGGCGTGTGGGAGCATCACGATCGCGGCGCGTGAGTTGGGGTTCAACCCGGCGACCTGCGGGACGTG
GGTATGGAAGGCCGGCCTGCGCAGCCAGGGGAAAACCGGGACTGGTCCGCATCCAGGAAAGGAACGCTACTTCCAGTTGCGACGCGATGGCATCTCGCGT
CGCGAAGCAGCGGCTGCCGTCGGGGTGAACATCCGCACGGCACGGGATTGGGATAACGGTGTCCGGAAGACCGCTCATCGCCGCTATTACCCAGATGGTC
GGGTCGTCGATTACAAAACAGGTATGACGACGTTTCTTGACGGCACCGAGACAATCTCGCCGGCGGCGATCTTTCAGCTGGAGAGGAAGCTCGATCCGCG
GTTCCTTTCGCTGGAGGAACGTGAGCAGATCCGGGATCTCCTCCGCGATGGGTTGTCACTGCGGAGCATCGCCGCCCAGCTGCGGCGGTCGCCATCCACG
ATCAGTCGGGAAATCTCCCGGAACCGGTCCTCGACCGGGATCTATCACCCCTTCGCAGCACATCGCTACTCGGCGAAGCGTCGCCCACGGCCAAAGCCGC
GAAGACTGGTGACAGAGCTGCGGTTGCGTGCTTTCGTTGAGTCGAAGCTGGCGTTGCGGTGGTCTCCCGAGCAGATCACTCGAGCCCTGATCCGTCAGTT
CCCGGACGACGTTGGGATGCGAGTGGCGACCGAGACGATCTACCAGACGCTCTATCTCCAAGGACGCGGCCAGCTGCGCCGTGATCTCGCGACAGCGCTG
CGTACCGGGCGCGCACGTCGCCGACCGAACCGGGGAACCAACGCACGTCGTTCGCGGTTCGTCGACCCGATGCTCATGATCTCCGAGCGCCCCGCTGAGG
TCGCTGATCGTGCTGTTCCAGGGCATTGGGAAGGCGATCTCATCATCGGCGCTGACCACGCCAGCGCGATCGGCACCCTCGTCGAGCGAACCACCAGGTT
CGTGATGCTCGTCCACCTCCCGACCGACCACGCTGCCGAGACCGTCCGCGATGGCCTCATTCGGACGATGAGCGGCCTGCCGGCCGAACTGAAGAAGTCG
CTGACCTGGGACCAGGGTGCGGAGATGGCCGCCCACAAGACGTTCACGATCGCCACCGACATGGACGTCTACTTCTGCGACCCAGCCTCGCCCTGGCAGC
GCGGTTCTAACGAGAACACGAACGGGTTGCTGAGGCAGTACTTCCCGAAGGGCACCGACCTCTCACAGCACTCGCCCGCGGACCTTGCCCGAGTCGCACA
CGAACTCAACACCCGCCCACGCAAAACGCTCGGCTGGGAAACCCCAGCCCAGCGCCTCGCTAAACTACCTACCAGCTAATCGTCGTGTTGCAACGACCAC
TAGAATCCGCC
AGGCTGATAAGGACGCGTTCTTCGCGTTGTTCGAGGCGTGTGGGAGCATCACGATCGCGGCGCGTGAGTTGGGGTTCAACCCGGCGACCTGCGGGACGTG
GGTATGGAAGGCCGGCCTGCGCAGCCAGGGGAAAACCGGGACTGGTCCGCATCCAGGAAAGGAACGCTACTTCCAGTTGCGACGCGATGGCATCTCGCGT
CGCGAAGCAGCGGCTGCCGTCGGGGTGAACATCCGCACGGCACGGGATTGGGATAACGGTGTCCGGAAGACCGCTCATCGCCGCTATTACCCAGATGGTC
GGGTCGTCGATTACAAAACAGGTATGACGACGTTTCTTGACGGCACCGAGACAATCTCGCCGGCGGCGATCTTTCAGCTGGAGAGGAAGCTCGATCCGCG
GTTCCTTTCGCTGGAGGAACGTGAGCAGATCCGGGATCTCCTCCGCGATGGGTTGTCACTGCGGAGCATCGCCGCCCAGCTGCGGCGGTCGCCATCCACG
ATCAGTCGGGAAATCTCCCGGAACCGGTCCTCGACCGGGATCTATCACCCCTTCGCAGCACATCGCTACTCGGCGAAGCGTCGCCCACGGCCAAAGCCGC
GAAGACTGGTGACAGAGCTGCGGTTGCGTGCTTTCGTTGAGTCGAAGCTGGCGTTGCGGTGGTCTCCCGAGCAGATCACTCGAGCCCTGATCCGTCAGTT
CCCGGACGACGTTGGGATGCGAGTGGCGACCGAGACGATCTACCAGACGCTCTATCTCCAAGGACGCGGCCAGCTGCGCCGTGATCTCGCGACAGCGCTG
CGTACCGGGCGCGCACGTCGCCGACCGAACCGGGGAACCAACGCACGTCGTTCGCGGTTCGTCGACCCGATGCTCATGATCTCCGAGCGCCCCGCTGAGG
TCGCTGATCGTGCTGTTCCAGGGCATTGGGAAGGCGATCTCATCATCGGCGCTGACCACGCCAGCGCGATCGGCACCCTCGTCGAGCGAACCACCAGGTT
CGTGATGCTCGTCCACCTCCCGACCGACCACGCTGCCGAGACCGTCCGCGATGGCCTCATTCGGACGATGAGCGGCCTGCCGGCCGAACTGAAGAAGTCG
CTGACCTGGGACCAGGGTGCGGAGATGGCCGCCCACAAGACGTTCACGATCGCCACCGACATGGACGTCTACTTCTGCGACCCAGCCTCGCCCTGGCAGC
GCGGTTCTAACGAGAACACGAACGGGTTGCTGAGGCAGTACTTCCCGAAGGGCACCGACCTCTCACAGCACTCGCCCGCGGACCTTGCCCGAGTCGCACA
CGAACTCAACACCCGCCCACGCAAAACGCTCGGCTGGGAAACCCCAGCCCAGCGCCTCGCTAAACTACCTACCAGCTAATCGTCGTGTTGCAACGACCAC
TAGAATCCGCC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1056 bp | 351 aa | 424 | 1479 | + | No |
Chemistry : DDE
ORF sequence :
MTTFLDGTETISPAAIFQLERKLDPRFLSLEEREQIRDLLRDGLSLRSIAAQLRRSPSTISREISRNRSSTGIYHPFAAHRYSAKRRPRPKPRRLVTELR
LRAFVESKLALRWSPEQITRALIRQFPDDVGMRVATETIYQTLYLQGRGQLRRDLATALRTGRARRRPNRGTNARRSRFVDPMLMISERPAEVADRAVPG
HWEGDLIIGADHASAIGTLVERTTRFVMLVHLPTDHAAETVRDGLIRTMSGLPAELKKSLTWDQGAEMAAHKTFTIATDMDVYFCDPASPWQRGSNENTN
GLLRQYFPKGTDLSQHSPADLARVAHELNTRPRKTLGWETPAQRLAKLPTS
LRAFVESKLALRWSPEQITRALIRQFPDDVGMRVATETIYQTLYLQGRGQLRRDLATALRTGRARRRPNRGTNARRSRFVDPMLMISERPAEVADRAVPG
HWEGDLIIGADHASAIGTLVERTTRFVMLVHLPTDHAAETVRDGLIRTMSGLPAELKKSLTWDQGAEMAAHKTFTIATDMDVYFCDPASPWQRGSNENTN
GLLRQYFPKGTDLSQHSPADLARVAHELNTRPRKTLGWETPAQRLAKLPTS
Blast result :
Comments
No DR was identified in this element. Eight copies of ISLxc3 were sequenced, but no direct repeat was found in any of them. It is possible to speculate that the three first nucleotides of 5' IR (CTT) and the three first nucleotides of 3' IR (GGC (reverse complement of GCC)) are actually not part of IR, since they are not complementary. The sequence CTTGCC might be part of the host genome and be recognized as the target site for IS insertion, but none experimental analysis was carried out.
ISLxc3 is 73% aa similar to ISLxx5.
ISLxc3 is 73% aa similar to ISLxx5.
References
1] Zerillo,M.M. (2008) Direct submission.