ISMsm7
- Family IS3
- Group IS3
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_008596 | ND | Mycobacterium smegmatis | Mycobacterium smegmatis MC2 155 |
DNA section
IS Length : 1279 bp
Ends
IR Length : 32/44
IRL : TGAAAGGGGACTGCCCCCTGTTTGGTTGACTCCTGACCTGTGAGGATTCG
IRR : TGAAGTTGGGACTGCCCCGGGTTCAGTTGACTCGCGGGGTGTGAATTTCA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GGCCTTCCTTCTC | GGGCAGACTGGGC | 0 |
DNA sequence
TGAAAGGGGACTGCCCCCTGTTTGGTTGACTCCTGACCTGTGAGGATTCGTCCTTGCTGGAAGGATCAATCTCGTGCCGACACCGTTTCCTGCCGAGTTC
CGTGCCGACGTCATCGCCGTGGCCCGCAAGGGTGAGGCGCCGCTGCGCCAGATCGCGAAGGACTTCGGAATCTCGGAGGCCTGCCTGCATCGCTGGCTCA
AGATCGCTGACCGTGAGGATGGCCGCGGCCAGCCCGCGTCACCGGCCGATGCTGACGACATGGCCGCGCAGCTGCGCGAAGCACACAAACGGATCAAACT
CCTCGAGCAAGAGGCTGAGGTGATGCGACGCGCGGTCGGCTACCTATCCCGGGACGCCAACCCAAAATGATGTACCCGCTGGTCCTCGACCTTGCCGCTG
ACGGCGTGCCCGTCACGGTGACCTGCCGGGTTCTGGGGTTCTCCACCCAGGCGTTCTACAGATGGCGTAAAGCGCCTGTCTCGCAGCGGGATTGGGACGA
TGCGCACCTGATCAATGCCGCGCGCAATATCCACGCCGATGATCCTGCGTTCGGCTATCGGTTCATCGCCGACGAACTTCCCGCGCATGGGATCACCGCC
GGTGAGAACCGGGTGGCACGGCTGTGTTCCCAGGAACGGATCTGGTCGATCTTCGCCAAGAAGCGTGGACTCAACCGCCGATCCGGACCTCCCGTCCATG
ACGACCTGGTGCAACGTCAGTTCAGCACCCACGCTCCCAACCAGGTCTGGCTGGCCGACATCACCGAGCATCGCACCGACGAAGGCAAGCTCTACCTGTG
CGCCCTCAAAGACGTCTACTCCAACCGGATTGTCGGCTACTCGATCGACTCTCGAATGAAGTCTTCGTTGGCCGTAGCAGCACTGGAGCACGCTGTGGCG
CTGCGCTCACCGGTTGCGACGATCGTGCACTCCGACAGGGGCAGTCAGTTCCGGTCGCGAAAGTTCGTTCACGCACTGTCGCACAACGGGTTACACGGAT
CGATGGGCCGCGTCGGCGCCTGCGGAGACAACGCCGCCATGGAGTCGTTCTTCGCCCTGTTGCAACGCAACGTCCTGGATCGGCGACGCTGGACAACCCG
CGCCGAACTCCGCCTGGCAATCGTCACCTGGATCGAGCGGACCTACCATCGCCGCCGGCGCCAACGCGCGCTCGGCAGGCTCACCCCGATAGAGTTCGAA
CTGCTCCACACACCAGTCGCAACCGCGGCCTGAAATTCACACCCCGCGAGTCAACTGAACCCGGGGCAGTCCCAACTTCA
CGTGCCGACGTCATCGCCGTGGCCCGCAAGGGTGAGGCGCCGCTGCGCCAGATCGCGAAGGACTTCGGAATCTCGGAGGCCTGCCTGCATCGCTGGCTCA
AGATCGCTGACCGTGAGGATGGCCGCGGCCAGCCCGCGTCACCGGCCGATGCTGACGACATGGCCGCGCAGCTGCGCGAAGCACACAAACGGATCAAACT
CCTCGAGCAAGAGGCTGAGGTGATGCGACGCGCGGTCGGCTACCTATCCCGGGACGCCAACCCAAAATGATGTACCCGCTGGTCCTCGACCTTGCCGCTG
ACGGCGTGCCCGTCACGGTGACCTGCCGGGTTCTGGGGTTCTCCACCCAGGCGTTCTACAGATGGCGTAAAGCGCCTGTCTCGCAGCGGGATTGGGACGA
TGCGCACCTGATCAATGCCGCGCGCAATATCCACGCCGATGATCCTGCGTTCGGCTATCGGTTCATCGCCGACGAACTTCCCGCGCATGGGATCACCGCC
GGTGAGAACCGGGTGGCACGGCTGTGTTCCCAGGAACGGATCTGGTCGATCTTCGCCAAGAAGCGTGGACTCAACCGCCGATCCGGACCTCCCGTCCATG
ACGACCTGGTGCAACGTCAGTTCAGCACCCACGCTCCCAACCAGGTCTGGCTGGCCGACATCACCGAGCATCGCACCGACGAAGGCAAGCTCTACCTGTG
CGCCCTCAAAGACGTCTACTCCAACCGGATTGTCGGCTACTCGATCGACTCTCGAATGAAGTCTTCGTTGGCCGTAGCAGCACTGGAGCACGCTGTGGCG
CTGCGCTCACCGGTTGCGACGATCGTGCACTCCGACAGGGGCAGTCAGTTCCGGTCGCGAAAGTTCGTTCACGCACTGTCGCACAACGGGTTACACGGAT
CGATGGGCCGCGTCGGCGCCTGCGGAGACAACGCCGCCATGGAGTCGTTCTTCGCCCTGTTGCAACGCAACGTCCTGGATCGGCGACGCTGGACAACCCG
CGCCGAACTCCGCCTGGCAATCGTCACCTGGATCGAGCGGACCTACCATCGCCGCCGGCGCCAACGCGCGCTCGGCAGGCTCACCCCGATAGAGTTCGAA
CTGCTCCACACACCAGTCGCAACCGCGGCCTGAAATTCACACCCCGCGAGTCAACTGAACCCGGGGCAGTCCCAACTTCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 297 bp | 98 aa | 73 | 369 | + | No |
Description : First part of the transposase
ORF sequence :
MPTPFPAEFRADVIAVARKGEAPLRQIAKDFGISEACLHRWLKIADREDGRGQPASPADADDMAAQLREAHKRIKLLEQEAEVMRRAVGYLSRDANPK
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 915 bp | 304 aa | 318 | 1232 | + | No |
Description : Second part of the transposase
ORF sequence :
GDATRGRLPIPGRQPKMMYPLVLDLAADGVPVTVTCRVLGFSTQAFYRWRKAPVSQRDWDDAHLINAARNIHADDPAFGYRFIADELPAHGITAGENRVA
RLCSQERIWSIFAKKRGLNRRSGPPVHDDLVQRQFSTHAPNQVWLADITEHRTDEGKLYLCALKDVYSNRIVGYSIDSRMKSSLAVAALEHAVALRSPVA
TIVHSDRGSQFRSRKFVHALSHNGLHGSMGRVGACGDNAAMESFFALLQRNVLDRRRWTTRAELRLAIVTWIERTYHRRRRQRALGRLTPIEFELLHTPV
ATAA
RLCSQERIWSIFAKKRGLNRRSGPPVHDDLVQRQFSTHAPNQVWLADITEHRTDEGKLYLCALKDVYSNRIVGYSIDSRMKSSLAVAALEHAVALRSPVA
TIVHSDRGSQFRSRKFVHALSHNGLHGSMGRVGACGDNAAMESFFALLQRNVLDRRRWTTRAELRLAIVTWIERTYHRRRRQRALGRLTPIEFELLHTPV
ATAA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1160 bp | 386 aa | 73 | 1232 | + | Yes |
Chemistry : DDE
ORF sequence :
MPTPFPAEFRADVIAVARKGEAPLRQIAKDFGISEACLHRWLKIADREDGRGQPASPADADDMAAQLREAHKRIKLLEQEAEVMRRAVGYLSRDANPKMM
YPLVLDLAADGVPVTVTCRVLGFSTQAFYRWRKAPVSQRDWDDAHLINAARNIHADDPAFGYRFIADELPAHGITAGENRVARLCSQERIWSIFAKKRGL
NRRSGPPVHDDLVQRQFSTHAPNQVWLADITEHRTDEGKLYLCALKDVYSNRIVGYSIDSRMKSSLAVAALEHAVALRSPVATIVHSDRGSQFRSRKFVH
ALSHNGLHGSMGRVGACGDNAAMESFFALLQRNVLDRRRWTTRAELRLAIVTWIERTYHRRRRQRALGRLTPIEFELLHTPVATAA
YPLVLDLAADGVPVTVTCRVLGFSTQAFYRWRKAPVSQRDWDDAHLINAARNIHADDPAFGYRFIADELPAHGITAGENRVARLCSQERIWSIFAKKRGL
NRRSGPPVHDDLVQRQFSTHAPNQVWLADITEHRTDEGKLYLCALKDVYSNRIVGYSIDSRMKSSLAVAALEHAVALRSPVATIVHSDRGSQFRSRKFVH
ALSHNGLHGSMGRVGACGDNAAMESFFALLQRNVLDRRRWTTRAELRLAIVTWIERTYHRRRRQRALGRLTPIEFELLHTPVATAA
Blast result :
Comments
ISMsm7 is 53% aa similar to ISCce4. The third ORF is a putative oRFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Fleischmann,R.D., Dodson,R.J., Haft,D.H., Merkel,J.S., Nelson,W.C. and Fraser,C.M. (2006) Direct Submission GenBank.