ISDet2
- Family IS3
- Group IS407
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_002936 | ND | Dehalococcoides ethenogenes | Dehalococcoides ethenogenes 195 |
DNA section
IS Length : 1417 bp
Ends
IR Length : 12/14
IRL : TGAAGTTGACCCTCCCCCCGTAATTAGTATCGCCGAGATGTAGAGTCCTC
IRR : TGAATGTGACCCTCTTCATTGCAAACCAAATGAGCTCGCCGAAGAACTTA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| ACAACGCTGC | GGTACACTGT | 0 |
DNA sequence
TGAAGTTGACCCTCCCCCCGTAATTAGTATCGCCGAGATGTAGAGTCCTCCGCTAAAATAAAACGTTCAAGGAGGGCTCAATTGATCAGGAAAAAGTTCA
CCGAAGAGCAGATCATCACCGTGCTAAAGGAAGCCGAAGCCGGGGCAAAGATCAGCGAGCTGTGCCGCAAGCACGGAGTCAGCGACGCCACTTACTACAA
GTGGAAAGCCAAATATGCGGGTCTTTCCATTAGCGAGCTTAAACGGCTCAAAACTCTGGAAGATGAAAACCGCCGTCTGAAGCAGATTGTGGCCGACCAG
GCGCTTGATAATTGGGCGCTTAAAGAGCTTCTCGCAAAAAACTTCTAGAGCCCAAGGTCAGGCGGGAGGCGGTGGAGTTTGCCAGGGGAAATCTTGGGCT
CAGTGAAAGAAGAGCTTGTCTGCTTGTCGGTATTTCGCCATCCGCCTACCGCTACAAGCCGAAACTAGACGATGATCTGGCCTTGCGCCAGCGGTTACGG
GATCTGGCCGGGGAGCGGAAGCGCTTTGGCAGCCCGCGGCTCCACATTATGCTTAAACGGGAAGGACTGGTGATCAATCACAAGCGGACCGAGCGAATCT
ACAAAGAAGAGGGATTGGCGTTAAGAAGAAAACGCAAACGCAAAGGAACTGCCGTTAATCGAATCATATTGCCGGTGCCCGATAGGCCTAACCAAAGATG
GAGCATGGACTTCGTTTCTGATTCCACCGTCACCGGGAGACGCTTCAGGACACTGACCATCGTCGATGACTTCACCCGGGAATGCCCAGCGATCGAAGTG
GATACATCTCTCGGTGGCGCCAGGGTGGTGAGTGTCCTGGAAAGGTTGGCTGAGACTCGAGGCTTGCCCGAAGTCATCACTATCGACAATGGACCTGAGT
TCGCCGGCCGGGCGCTTGATGAATGGGCTTATCGGCGGGGTGTAAAACTCAATTTCATACGGCCAGGCAAACCTATAGAAAATGCCTTCGCCGAGAGCTT
TAACGGTAGATTCAGGGACGAATGCCTGAATGAAAACTGGTTCATGAGCTTGAAACAAGCCCGGGAAATCATCGAGGATTGGAGGATCGACTATAACGAA
GTCAGGCCACACACTTCATTGAAAGGTCAAACACCTCAGGAGTATGCTGAGGCAAATTCTGGACTCTACTCCGGAATGCTACTAAAGGTGGGGTAAGGTC
AGCTTGACTAACGTTTAGTGGAAAACGCTCACTTCCATACGGTGCAACTCCAAGAATTCTTTTATTCAGCGATTGAGCAAAATTGATTTCAAATTGGATC
CAATCACTATGAGTAGCGTACATTCCACCCAAGATTATCGCCACTTCAACAGGCCTGATTTGTTGCCTAAGTTCTTCGGCGAGCTCATTTGGTTTGCAAT
GAAGAGGGTCACATTCA
CCGAAGAGCAGATCATCACCGTGCTAAAGGAAGCCGAAGCCGGGGCAAAGATCAGCGAGCTGTGCCGCAAGCACGGAGTCAGCGACGCCACTTACTACAA
GTGGAAAGCCAAATATGCGGGTCTTTCCATTAGCGAGCTTAAACGGCTCAAAACTCTGGAAGATGAAAACCGCCGTCTGAAGCAGATTGTGGCCGACCAG
GCGCTTGATAATTGGGCGCTTAAAGAGCTTCTCGCAAAAAACTTCTAGAGCCCAAGGTCAGGCGGGAGGCGGTGGAGTTTGCCAGGGGAAATCTTGGGCT
CAGTGAAAGAAGAGCTTGTCTGCTTGTCGGTATTTCGCCATCCGCCTACCGCTACAAGCCGAAACTAGACGATGATCTGGCCTTGCGCCAGCGGTTACGG
GATCTGGCCGGGGAGCGGAAGCGCTTTGGCAGCCCGCGGCTCCACATTATGCTTAAACGGGAAGGACTGGTGATCAATCACAAGCGGACCGAGCGAATCT
ACAAAGAAGAGGGATTGGCGTTAAGAAGAAAACGCAAACGCAAAGGAACTGCCGTTAATCGAATCATATTGCCGGTGCCCGATAGGCCTAACCAAAGATG
GAGCATGGACTTCGTTTCTGATTCCACCGTCACCGGGAGACGCTTCAGGACACTGACCATCGTCGATGACTTCACCCGGGAATGCCCAGCGATCGAAGTG
GATACATCTCTCGGTGGCGCCAGGGTGGTGAGTGTCCTGGAAAGGTTGGCTGAGACTCGAGGCTTGCCCGAAGTCATCACTATCGACAATGGACCTGAGT
TCGCCGGCCGGGCGCTTGATGAATGGGCTTATCGGCGGGGTGTAAAACTCAATTTCATACGGCCAGGCAAACCTATAGAAAATGCCTTCGCCGAGAGCTT
TAACGGTAGATTCAGGGACGAATGCCTGAATGAAAACTGGTTCATGAGCTTGAAACAAGCCCGGGAAATCATCGAGGATTGGAGGATCGACTATAACGAA
GTCAGGCCACACACTTCATTGAAAGGTCAAACACCTCAGGAGTATGCTGAGGCAAATTCTGGACTCTACTCCGGAATGCTACTAAAGGTGGGGTAAGGTC
AGCTTGACTAACGTTTAGTGGAAAACGCTCACTTCCATACGGTGCAACTCCAAGAATTCTTTTATTCAGCGATTGAGCAAAATTGATTTCAAATTGGATC
CAATCACTATGAGTAGCGTACATTCCACCCAAGATTATCGCCACTTCAACAGGCCTGATTTGTTGCCTAAGTTCTTCGGCGAGCTCATTTGGTTTGCAAT
GAAGAGGGTCACATTCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 267 bp | 88 aa | 82 | 348 | + | No |
Description : First part of the transposase
ORF sequence :
LIRKKFTEEQIITVLKEAEAGAKISELCRKHGVSDATYYKWKAKYAGLSISELKRLKTLEDENRRLKQIVADQALDNWALKELLAKNF
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 873 bp | 290 aa | 324 | 1196 | + | No |
Description : Second part of the transposase
ORF sequence :
RASRKKLLEPKVRREAVEFARGNLGLSERRACLLVGISPSAYRYKPKLDDDLALRQRLRDLAGERKRFGSPRLHIMLKREGLVINHKRTERIYKEEGLAL
RRKRKRKGTAVNRIILPVPDRPNQRWSMDFVSDSTVTGRRFRTLTIVDDFTRECPAIEVDTSLGGARVVSVLERLAETRGLPEVITIDNGPEFAGRALDE
WAYRRGVKLNFIRPGKPIENAFAESFNGRFRDECLNENWFMSLKQAREIIEDWRIDYNEVRPHTSLKGQTPQEYAEANSGLYSGMLLKVG
RRKRKRKGTAVNRIILPVPDRPNQRWSMDFVSDSTVTGRRFRTLTIVDDFTRECPAIEVDTSLGGARVVSVLERLAETRGLPEVITIDNGPEFAGRALDE
WAYRRGVKLNFIRPGKPIENAFAESFNGRFRDECLNENWFMSLKQAREIIEDWRIDYNEVRPHTSLKGQTPQEYAEANSGLYSGMLLKVG
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1109 bp | 371 aa | 88 | 1196 | + | Yes |
Chemistry : DDE
ORF sequence :
LIRKKFTEEQIITVLKEAEAGAKISELCRKHGVSDATYYKWKAKYAGLSISELKRLKTLEDENRRLKQIVADQALDNWALKELLAKKLLEPKVRREAVEF
ARGNLGLSERRACLLVGISPSAYRYKPKLDDDLALRQRLRDLAGERKRFGSPRLHIMLKREGLVINHKRTERIYKEEGLALRRKRKRKGTAVNRIILPVP
DRPNQRWSMDFVSDSTVTGRRFRTLTIVDDFTRECPAIEVDTSLGGARVVSVLERLAETRGLPEVITIDNGPEFAGRALDEWAYRRGVKLNFIRPGKPIE
NAFAESFNGRFRDECLNENWFMSLKQAREIIEDWRIDYNEVRPHTSLKGQTPQEYAEANSGLYSGMLLKVG
ARGNLGLSERRACLLVGISPSAYRYKPKLDDDLALRQRLRDLAGERKRFGSPRLHIMLKREGLVINHKRTERIYKEEGLALRRKRKRKGTAVNRIILPVP
DRPNQRWSMDFVSDSTVTGRRFRTLTIVDDFTRECPAIEVDTSLGGARVVSVLERLAETRGLPEVITIDNGPEFAGRALDEWAYRRGVKLNFIRPGKPIE
NAFAESFNGRFRDECLNENWFMSLKQAREIIEDWRIDYNEVRPHTSLKGQTPQEYAEANSGLYSGMLLKVG
Blast result :
Comments
ISDet2 (orfAB) is 76% aa similar to ISPae1.
IRR and IRL are not sure.
IRR and IRL are not sure.
References
1] Seshadri,R., Adrian,L., Fouts,D.E., Eisen,J.A., Phillippy,A.M., Methe,B.A., Ward,N.L., Nelson,W.C., Deboy,R.T., Khouri,H.M., Kolonay,J.F., Dodson,R.J., Daugherty,S.C., Brinkac,L.M., Sullivan,S.A., Madupu,R., Nelson,K.E., Kang,K.H., Impraim,M., Tran,K., Robinson,J.M., Forberger,H.A., Fraser,C.M., Zinder,S.H. and Heidelberg,J.F. (2005) Science 307 (5706), 105-108.