ISCpe2

  • Family IS200/IS605
  • Group IS605
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND Clostridium perfringens
Clostridium perfringens SM101
DNA section
IS Length : 1832 bp

Ends


Left end : GCCTTTGGCAAGAACACCCGTGATTTCAATCATGGGTGTTCTTGCCGCGACATATGCTAAAAGTATAAACCGTCTAACACTTGCAAATAAGTTATATTTT II struct. : Yes
Right end : GGTATCCAACGAGGTCGGAACGCCCTTGTTAGCTTGGTTAATCTTACACCGTTAGGTGTGTTGCCCAAGAATCTTGGCATTTCAATGCCAAGTAGTTCAA II struct. : Yes

Insertion site


Left flankLE cleavage siteRight flankRE cleavage site
TAAAAAGTAATTTTTTTTATTGAGGCTATAAGTGGTTCAA

DNA sequence

GCCTTTGGCAAGAACACCCGTGATTTCAATCATGGGTGTTCTTGCCGCGACATATGCTAAAAGTATAAACCGTCTAACACTTGCAAATAAGTTATATTTT
AGTTTTAAATATTTTACATATGTTAAATTAAATAATAATATAATTAAAGAGGTGGTAATAATGGCTCAAGTCAAGCAAGGCAGAGGTTATGTGTATTGTA
TTCAATATCATATAGTATGGTGTGTTAAATACAGAAGAAAAGTATTGTTTGGTGATGTGGATAAATCTCTTAAAGAAATAATATTAAAAATAGCAAATGA
TAATAATTTTGAAATTTCTGAAATGGAAACTGATAAAGACCATATACACCTGTTAATAGAATGTTCTCCACAACATCAAATTCCAAATATAATTAAAGCG
TTAAAGGGAGTATCTGCAAGGTTACTGTTCTTGAAACATCCTGAAATTAAAAGATATCTATGGGAAGGAAACCTATGGAATCCAAGCTATTTTGTAGCTA
CTGTATCAGAGAATACAGAAGAACAAATTAGAAACTATATTCAAAATCAACAAGTAAAGTGAGGTGAGATAAGTGATAAGAAAGAAAGCATATAAATTTA
GAATATACCCAACTAAGAAACAAGCCGAGCTAATTAACAAGACTATTGGATGTTGTAGGTTTGTATTCAACTATTCTTTAGGTGTTCAAAAAACTAAAGA
TAATTATTGGAATATAGTTGAGGAGATGGTTCAACAAGGCTACTTCCAAGAGAATAATTGGAAAGGCGAGTTCTTTAATAAAGCCAATTCAATTAAAGAT
ATAGCTAAATTAAAGAAAAGCTATGATTGGCTTAAAGAAGTAGATAGCATAGCACTACAGGCTTCTGTTGAAAATCTTGATGCTGGATATGATAAGTATT
ACAAAAAAACAGGTAGCAGACCTAAATTTAAGTCTAAGAAAAATGAAATACAATCTTATACTACTAAATTAGTAAAAGCTAAGGGTAATGTAAATATTGA
GATAGTAGGTAAAGGAATTAAACTACCTAAACTTGGATTAGTTAAGATAGAAAATTCAAGAAATGTAGATGGAAATATTAAAAGAGTTACTGTGAGCAGA
ACACAATCTGGTAAATATTTTGCATCTATTCTTTGTGATGTAAATATTCAAGAATTACCTAAAATAGATAAAAAAGTTGGTGTAGATGTTGGATTAAAAA
CTTTTGCTGTTTGTTCTGATGGATATGAAGAAGCTAATCCTAAACACTTTAGAAAGGCAGAAAAAAGACTTATTAAACTTCAAAGAGATTTAGCTCGTAA
AGAATATAACTCTAAAAACTATCACAAAAATAGAATTATGATTGCTAAATTACATGAAAGGATAGCAAATCAAAGAATGGACTTCTTACAAAAATTTTCA
ACTAAGCTAATTAGAGAAAACCAATCAATAGCCATTGAAGATTTAAGAGTTTCAAATATGCTTAAAAACCACAAACTTGCTAAAGTAATTTCAGAAGCAA
GTTGGTCAGAATTTAGAAGAATGCTAGAATATAAGGCTGAATGGTATGGTAGAGAAATAGTAATTGCTCCACCAGACTATGCAAGCTCTCAACTATGTTC
TGAATGTGGCTATAAGAATATAGAAGTTAAAAATCTAGGTCTAAGAGAATGGGTATGTCCCGAATGTGGAACACACCATCAAAGAGACCTTAATGCTTCA
ATAAACTTGGAAAAGCTGATAGCTTAAACTATTGGTATCCAACGAGGTCGGAACGCCCTTGTTAGCTTGGTTAATCTTACACCGTTAGGTGTGTTGCCCA
AGAATCTTGGCATTTCAATGCCAAGTAGTTCAA
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
402 bp132 aa160561+No
ORF function : Transposase
Chemistry : Y1

ORF sequence :

MAQVKQGRGYVYCIQYHIVWCVKYRRKVLFGDVDKSLKEIILKIANDNNFEISEMETDKDHIHLLIECSPQHQIPNIIKALKGVSARLLFLKHPEIKRYL
WEGNLWNPSYFVATVSENTEEQIRNYIQNQQVK

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
1155 bp384 aa5721726+No
ORF function : Accessory Gene
AG : TnpB

ORF sequence :

MIRKKAYKFRIYPTKKQAELINKTIGCCRFVFNYSLGVQKTKDNYWNIVEEMVQQGYFQENNWKGEFFNKANSIKDIAKLKKSYDWLKEVDSIALQASVE
NLDAGYDKYYKKTGSRPKFKSKKNEIQSYTTKLVKAKGNVNIEIVGKGIKLPKLGLVKIENSRNVDGNIKRVTVSRTQSGKYFASILCDVNIQELPKIDK
KVGVDVGLKTFAVCSDGYEEANPKHFRKAEKRLIKLQRDLARKEYNSKNYHKNRIMIAKLHERIANQRMDFLQKFSTKLIRENQSIAIEDLRVSNMLKNH
KLAKVISEASWSEFRRMLEYKAEWYGREIVIAPPDYASSQLCSECGYKNIEVKNLGLREWVCPECGTHHQRDLNASINLEKLIA

 

Blast result :
Comments
ISCpe2 is 72%(ORFA) and 57%(ORFB) aa similar to ISDra2.
References
1] Myers,G.S., Rasko,D.A., Cheung,J.K., Ravel,J., Seshadri,R., DeBoy,R.T., Ren,Q., Varga,J., Awad,M.M., Brinkac,L.M., Daugherty,S.C., Haft,D.H., Dodson,R.J., Madupu,R., Nelson,W.C., Rosovitz,M.J., Sullivan,S.A., Khouri,H., Dimitrov,G.I., Watkins,K.L., Mulligan,S., Benton,J., Radune,D., Fisher,D.J., Atkins,H.S., Hiscox,T., Jost,B.H., Billington,S.J., Songer,J.G., McClane,B.A., Titball,R.W., Rood,J.I., Melville,S.B. and Paulsen,I.T.
(2006) Genome Res. 16 (8), 1031-1040