ISSsp5

  • Family IS21
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
AB270530 ND Sphingomonas sp.
Sphingomonas sp. KA1 plasmid pCAR3
DNA section
IS Length : 2501 bp

Ends


IR Length : 25/30

IRL : TGAGAACGGCGGAGCAATATTCGGCCACGCTAGCGGCGGGATAGTCCTGC
IRR : TGTCAACGGCGGAGCAAAAGTCGGCCATGCGGCGGCGTAAAACCAGGCCA

Insertion site


Left flankDirect repeatRight flankDR Length
ATGAGCTTGTAGAGCAGGCG0

DNA sequence

TGAGAACGGCGGAGCAATATTCGGCCACGCTAGCGGCGGGATAGTCCTGCTGCGGGCGGCGTAAAAGTCGTCCACCTTGAAGCCTTTCTGCCAAGTCAGG
GAGGTGTGGGGATCTACAGCGTGGAACTTTATCTTCAGGTCCGTTTGGCTTGCGCGGATGGCATGAGCCAGCGGGCGGCGGCGAAGCGTTTCAATGTGTC
GCGCGATACGGTGCGCAAGATGCTGTCGTTTTCATCGCCGCCGGGTTACCGGCGTCAGTCTGCACCGCAGCGCCCGAAGCTGGACGGGTTTGTGGGGATC
ATCGATGGATGGCTTGAGGGGGATCGCGGTGTCCCGCGCAAGCAGCGCCATACGGCGAAGCGGGTATTCGACCGTTTGCGCACCGAGCATGGTTTCACCG
GCGGCTATACGATCATCAAGGATTACATCCGGGAGCGCGAGCAGCGCAGCCGGGAGATGTTCGTGCCGCTGGCGCACCCGGCGGGAGATGCACAGGCCGA
TTTCGGGGAAGCTCTGGTGGAGATCGGCGGGGTGGAGCAAAAAGCCTACTTCTTCGCGCTCGATCTGCCGCACAGTGATGCCTGCTATGTGCGAGCCTAT
CCGGCGGCGGTGGCGGAGGCCTGGGTGGACGGACATGTCCATGCCTTCGCGTTCTTCGGCGCGGTGCCGCGCTCGATCGTCTATGACAACGATCGCTGCC
TTGTGGCGAAAATCCTGCCAGACGGCACGCGCAAGCGTGCCACGCTGTTCAGCGCTTTCCTGTCGCATTACGTGATCCGCGACCGCTATGCCCGCCCGGG
CAAGGGGAACGAGAAAGGCAATGTGGAAGGGCTGGTAGGTTACTGCCGCCGCAATTTCATGGTGCCGATCCCGAAGTTCCCGACCTGGGAGGCCTTCAAC
CTGTGGCTGGAGGAGCAATGCCGCAAGCGCCAGCAGGACAAGGTGCGCGGGGAGAGCGAGACGATCGGTGAGCGCTTGCAGCGCGATCTGGCGGCCATGC
AGCCTCTGCCCGCTACACCCTTCGAGGCCTGCGATCAGACGAGCGGGCGGGTCTCCTCGCAATCTCTGGTGCGCTACAAGACCAACGATTATTCGGTTCC
GGTAGTCTGGGGCCATCAGGAGGTCTGGATCAGGGCCTATGTCGATGAAGTGGTGATCGGATGCCGCAGCGAAGTTATCGCCCGTCATCCTCGTTGCTAT
GCCCGCGAGGAGGTTATCTTCGCCCCGCTCCACTATCTCCCGCTGGTCGAGCAGAAGATCAACGCATTCGACCAGGCCGCCCCTTTGCAGGGCTGGGATT
TGCCCGAAGCGTTCGGGACGCTGCAGCGGTTGATGGAAGGGCGCATGCACAAACATGGCAGGCGCGAATATGTGCAGGTGCTGCGCCTGCTGGAAACGTT
CACCCTCGCCGATCTCCAGGCGGCGGTGGAACAGGCCATCGCCCTTGGCGCCATCGGCTTCGATGCCGTCAAGCACCTCGTCCTGTGCCGGGTCGAACGC
GTACCGCCCAGACTGGACCTGGACATCTATCCCTTCCTGCCTCGCACCACGGTCGAGAAGACCGTTGCCAGAGCCTATCTGAGCCTGCTCTGCGACAGGC
AGGAGGCCGCATGAGCGATCAGGCACCGGAGATTCTTCTCGCTCACCATCTCAAGGCCCTCAAGCTGCCTACGTGCCTGCGAGAGCATCACAAGCTCGCC
CGGCAATGTGCCGCTGAAGGCGTCGATCATATCCGCTTCCTCGCCCGTCTCGTCGAGATGGAGATGATCGACAGGGAGCGTCGTATGGTCGAGCGGCGCA
TCAAGGCCGCGCGCTTCCCCGCCGTCAAAAGCCTCGATAGCTTCGACTTCGCCGTCATCCCCAGGCTCAACAAGATGCAGGTGCTCGAGATGGCGCGCTG
CGAGTGGATCGAGCGGCGTGAGAACGCCATCGCTCTGGGGCCATCGGGCACCGGGAAGACGCACGTAGCTTTGGGGCTCGGACTGGCAGCATGCCAGAAA
GGACTGTCGGTGGGCTTCACCACTGCGGCGGCGCTGGTCAGCGAGATGATGGAGGCGCGCGACGAGCGGCGTCTCCTGCGCTTCCAGAAGCAGATGGCCG
GATACAAGCTGCTCATCATTGACGAACTGGGCTTCGTACCGCTCTCCAAGACCGGTGCCGAACTGTTGTTCGAGCTGATCTCCCAGCGCTATGAGCGCGG
CTCCACCTTGATCACCAGCAACCTGCCCTTCGACGAATGGACCGAAACCTTCGGATCCGAGCGCCTCACAGGCGCGCTCCTCGATCGTCTGACCCATCAC
GTCAGCATCCTCGAGATGAACGGCGAAAGCTATCGCCTCGCTCACAGCCGGGCCCGAAAGGCCAAAACCAGACCCTGAAAATCAAGCCAATGCCGGGGGG
AGTGGCCTTCGGGCTACGCCCTCACGCCACTCCCCCCGGCATGTAACACCGTGGCCTGGTTTTACGCCGCCGCATGGCCGACTTTTGCTCCGCCGTTGAC
A
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
1494 bp497 aa1211614+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MELYLQVRLACADGMSQRAAAKRFNVSRDTVRKMLSFSSPPGYRRQSAPQRPKLDGFVGIIDGWLEGDRGVPRKQRHTAKRVFDRLRTEHGFTGGYTIIK
DYIREREQRSREMFVPLAHPAGDAQADFGEALVEIGGVEQKAYFFALDLPHSDACYVRAYPAAVAEAWVDGHVHAFAFFGAVPRSIVYDNDRCLVAKILP
DGTRKRATLFSAFLSHYVIRDRYARPGKGNEKGNVEGLVGYCRRNFMVPIPKFPTWEAFNLWLEEQCRKRQQDKVRGESETIGERLQRDLAAMQPLPATP
FEACDQTSGRVSSQSLVRYKTNDYSVPVVWGHQEVWIRAYVDEVVIGCRSEVIARHPRCYAREEVIFAPLHYLPLVEQKINAFDQAAPLQGWDLPEAFGT
LQRLMEGRMHKHGRREYVQVLRLLETFTLADLQAAVEQAIALGAIGFDAVKHLVLCRVERVPPRLDLDIYPFLPRTTVEKTVARAYLSLLCDRQEAA

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
768 bp255 aa16112378+No
ORF function : Accessory Gene
AG : IS21 helper

ORF sequence :

MSDQAPEILLAHHLKALKLPTCLREHHKLARQCAAEGVDHIRFLARLVEMEMIDRERRMVERRIKAARFPAVKSLDSFDFAVIPRLNKMQVLEMARCEWI
ERRENAIALGPSGTGKTHVALGLGLAACQKGLSVGFTTAAALVSEMMEARDERRLLRFQKQMAGYKLLIIDELGFVPLSKTGAELLFELISQRYERGSTL
ITSNLPFDEWTETFGSERLTGALLDRLTHHVSILEMNGESYRLAHSRARKAKTRP

 

Blast result :
Comments
ISSsp5 is 67%(istA) and 80%(istB) aa similar to ISFlsp1.
L1 : GGCGGCGTAAAAGTCG
R1 : GGCGGAGCAAAAGTCG
R2 : GGCGGCGTAAAA
References
1] Habe,H., Ashikawa,Y., Saiki,Y., Yoshida,T., Nojiri,H. and Omori,T.(2002) FEMS Microbiol. Lett. 211 (1), 43-49.

2] Inoue,K., Widada,J., Nakai,S., Endoh,T., Urata,M., Ashikawa,Y., Shintani,M., Saiki,Y., Yoshida,T., Habe,H., Omori,T. and Nojiri,H.(2004)Biosci. Biotechnol. Biochem. 68 (7), 1467-1480.

3] Inoue,K., Habe,H., Yamane,H., Omori,T. and Nojiri,H.(2005)FEMS Microbiol. Lett. 245 (1), 145-153.

4] Urata,M., Uchimura,H., Noguchi,H., Sakaguchi,T., Takemura,T., Eto,K., Habe,H., Omori,T., Yamane,H. and Nojiri,H.(2006)Appl. Environ. Microbiol. 72 (5), 3198-3205.

5] Shintani,M., Urata,M., Inoue,K., Eto,K., Habe,H., Omori,T., Yamane,H. and Nojiri,H.(2007)J. Bacteriol. 189 (5), 2007-2020.