ISSsp4
- Family IS21
- Group
Isoform Synonym(s)
Accession number | Transposition | Origin | Host |
---|---|---|---|
AB270530 | ND | Sphingomonas sp. | Sphingomonas sp. KA1 plasmid pCAR3 |
DNA section
IS Length : 2558 bp
Ends
IR Length : 24/29
IRL : TGTGGAGCGGCGTGCAAAATTGACCCCCTTAGCGGGGTGATCGGCGTCTA
IRR : TGTCAACCGGCGCGCAAAACTGACCCCCTATCGGCGCCCAATATTGACCC
Insertion site
Left flank | Direct repeat | Right flank | DR Length |
---|---|---|---|
GCCACTACAA | CCTACAACAC | 0 |
DNA sequence
TGTGGAGCGGCGTGCAAAATTGACCCCCTTAGCGGGGTGATCGGCGTCTAAAATTGACCCCCTTCATCTCATCATGAGAAGCGCCGCCGTTTGGGTTCCG
GACGGCGGGTACAGGGATGTTGGTTGTGGAGACGATTGCGAGGATCCGTCGGGAGCACCGGGACGGGAAGCCTATCAAGGCGATAGCGCGGGATTTGCGG
CTATCGCGCAACACGGTGCGCAAGGCGGTCCGGGCACCGGACGCGGACGCCAGTTACGAGCGTAAGGAACAGCACCGGCCGCGGACCGGACCTTTCAGTG
CTCGTCTTGAAGAATTGCTCGAGCAGAATGAAGGGCTGCCCCGGCGTGACCGGCTTCGTATGACGAGGATCCATGATCTGCTTCAGCGCGAAGGGTTCGG
CGGCTCCTATGACGCTGTGCGGCGCTATGCCGCGCGCTGGAAGCGTGAGCGTCGCGCCGACGCCGGCGATATGGGGAAGGTGTTCATCCCATTGATGTAC
AGGCCAGGCGAGGCTTACCAGTTCGACTGGAGCCACGAGGATGTCGAGATCGCGGGCAAGCCGATGCGGGTGAAGGTCGCTCATATGCGATTATGCTGGT
CACGGGCACCATTCGTCCGTGCATATCCTCGCGAGACGCAGGAGATGGTGTTCGACGCACATTCCCGCGCCTTTGCGTTCTTCGGCGGGGTTCCGACGCG
CGGCATCTACGACAATATGAAGACGGCTGTGACGACGGTGTTCACCGGCAAGGAACGCCTGTTCAACCGGCGGTTCCTGATCATGACGGACCACTATCGC
GTCGAGCCGGTGGCCTGCAGTCCTGCCGCAGGGTGGGAGAAGGGGCAGGTCGAGAACCAGGTTCAGACTAGCAGGGAGCGCCTGTTCAAGCCGCGCCTGC
GCTTTGCCAGCCTCGAGGAGCTGAACATCTGGCTGGAGGCCGAGTGCCGTCGCTGGGCGGAACGCAACCAGCATCCTGACCAGGCAGATATGACGATCGC
GCAGGCGCTGGAGGCGGAGCTTCCCATGCTGCAACCGCTACCGGTCCCCTTCGACGGGTTCTTCGAGAGCGAACACGTAGCCAGTTCGACGTGCCTCGTG
AGCTTCGATCGCAATCGGTATTCGGTGATGGCAAAGGCGGCCCATCAAGCTGTGCAGGTGCGCGCCTACGCAACGCGCATCGTGATCCGCTGCGACGGCG
CTGTCGTTGCCGAACACGAACGGGTCTTCGGTCGGAACCAGACGATCTTCGATCCGTGGCATTACCTGCCCATCCTCGCCCGCAAGCCCGGTGCGTTGCG
CAATGGCGCCCCCTTCCAGGACTGGGCTCTCCCACCTGCACTGGCCCAACTGCGGCGCAAGCTGGGCAAGGGCGATGATGCGGATCGCCGCTTCGTCCGC
GTCCTGGCCGCCATTCCCGACGACGGCCTCGAGGTTGTTGAAGCCGCAGTCGCCGAGGCCCTCAGCGCAGGAACGGTCAGCGACGAGGTCATCCTCAACA
TCCTGTCCCGCCGGCGTGAGCCTCGCGATGCCCAGACCATGGACGTCGTCGTCAACCTGAAGCTCAAGCATCCGCCGATTGCAGACTGCGCCCGCTACGA
CACGGTGCGAGGCCTCAATGCAGCGGCATGAGATTGTGGCAGCCCTCAAGGGGCTGGGGCTCAAGGGCATGGTCACTGCCTTCGATGATGCCGTCACCAA
TGGCATCCGCCGCGACAGAACGATCATGGAGATGCTGGCCGACCTGCTGCGTGCCGAGACTGCACATCGGGAGGCAGCGTCGATCCGCTACCGGATGACC
GCGGCGAAGCTTCCGGCAATCAAGGACCTGGATGGGTTCATGTTCGACGGAACGCCGATCAACGAAGGGCTGATCCGCTCCCTGCACGCCGGCTCCTTCC
TGCCGGAACGGCGCAATATCGTGCTGGTAGGTGGAACGGGCACGGGCAAGACCCACCTGGCGCTCGCCATCACTGCCGCAGTGGTACGCGCCGGCACCAG
AGGCCGATACTTCAATACGGTTGATCTGGTGAACCGGCTTGAGGAAGAAGCACGACTGGGCAAAGCCGGAAGCCTTGCCGCTCAACTCTCACGGCTTGAT
CTCGTTGTGCTCGATGAACTGGGCTATCTCCCGTTCGCCCGTTCCGGCGGCCAGATGCTCTTCCACCTGATCAGCAAGCTCTACGAAAAGACCTCGGTGA
TCATCACGACCAATCTCGCCTTTGGCGAATGGCCGAGCGTCTTCCACGACGCCAAGATGACCACCGCGCTCCTTGATCGCATCACCCACCATTGCGACAT
CATCGAGACAGGCAACGACAGCTGGCGGTTCAAAAACCGAAACTGAGCCCAAAACCCCTTCCCCCGGACCCCCATCCCCGGCGGTTAACCCGGTGGGTCA
ACAGGGACCTCCCACGGACGCCGCCGCCAGCACACCTGACGGTGCGCGTCGTCGTCCGTGGGCCCCTCCTATCGACTACCGGGATAACCGCCGCGCTGCC
GAAAGGTGGGGTCAATATTGGGCGCCGATAGGGGGTCAGTTTTGCGCGCCGGTTGACA
GACGGCGGGTACAGGGATGTTGGTTGTGGAGACGATTGCGAGGATCCGTCGGGAGCACCGGGACGGGAAGCCTATCAAGGCGATAGCGCGGGATTTGCGG
CTATCGCGCAACACGGTGCGCAAGGCGGTCCGGGCACCGGACGCGGACGCCAGTTACGAGCGTAAGGAACAGCACCGGCCGCGGACCGGACCTTTCAGTG
CTCGTCTTGAAGAATTGCTCGAGCAGAATGAAGGGCTGCCCCGGCGTGACCGGCTTCGTATGACGAGGATCCATGATCTGCTTCAGCGCGAAGGGTTCGG
CGGCTCCTATGACGCTGTGCGGCGCTATGCCGCGCGCTGGAAGCGTGAGCGTCGCGCCGACGCCGGCGATATGGGGAAGGTGTTCATCCCATTGATGTAC
AGGCCAGGCGAGGCTTACCAGTTCGACTGGAGCCACGAGGATGTCGAGATCGCGGGCAAGCCGATGCGGGTGAAGGTCGCTCATATGCGATTATGCTGGT
CACGGGCACCATTCGTCCGTGCATATCCTCGCGAGACGCAGGAGATGGTGTTCGACGCACATTCCCGCGCCTTTGCGTTCTTCGGCGGGGTTCCGACGCG
CGGCATCTACGACAATATGAAGACGGCTGTGACGACGGTGTTCACCGGCAAGGAACGCCTGTTCAACCGGCGGTTCCTGATCATGACGGACCACTATCGC
GTCGAGCCGGTGGCCTGCAGTCCTGCCGCAGGGTGGGAGAAGGGGCAGGTCGAGAACCAGGTTCAGACTAGCAGGGAGCGCCTGTTCAAGCCGCGCCTGC
GCTTTGCCAGCCTCGAGGAGCTGAACATCTGGCTGGAGGCCGAGTGCCGTCGCTGGGCGGAACGCAACCAGCATCCTGACCAGGCAGATATGACGATCGC
GCAGGCGCTGGAGGCGGAGCTTCCCATGCTGCAACCGCTACCGGTCCCCTTCGACGGGTTCTTCGAGAGCGAACACGTAGCCAGTTCGACGTGCCTCGTG
AGCTTCGATCGCAATCGGTATTCGGTGATGGCAAAGGCGGCCCATCAAGCTGTGCAGGTGCGCGCCTACGCAACGCGCATCGTGATCCGCTGCGACGGCG
CTGTCGTTGCCGAACACGAACGGGTCTTCGGTCGGAACCAGACGATCTTCGATCCGTGGCATTACCTGCCCATCCTCGCCCGCAAGCCCGGTGCGTTGCG
CAATGGCGCCCCCTTCCAGGACTGGGCTCTCCCACCTGCACTGGCCCAACTGCGGCGCAAGCTGGGCAAGGGCGATGATGCGGATCGCCGCTTCGTCCGC
GTCCTGGCCGCCATTCCCGACGACGGCCTCGAGGTTGTTGAAGCCGCAGTCGCCGAGGCCCTCAGCGCAGGAACGGTCAGCGACGAGGTCATCCTCAACA
TCCTGTCCCGCCGGCGTGAGCCTCGCGATGCCCAGACCATGGACGTCGTCGTCAACCTGAAGCTCAAGCATCCGCCGATTGCAGACTGCGCCCGCTACGA
CACGGTGCGAGGCCTCAATGCAGCGGCATGAGATTGTGGCAGCCCTCAAGGGGCTGGGGCTCAAGGGCATGGTCACTGCCTTCGATGATGCCGTCACCAA
TGGCATCCGCCGCGACAGAACGATCATGGAGATGCTGGCCGACCTGCTGCGTGCCGAGACTGCACATCGGGAGGCAGCGTCGATCCGCTACCGGATGACC
GCGGCGAAGCTTCCGGCAATCAAGGACCTGGATGGGTTCATGTTCGACGGAACGCCGATCAACGAAGGGCTGATCCGCTCCCTGCACGCCGGCTCCTTCC
TGCCGGAACGGCGCAATATCGTGCTGGTAGGTGGAACGGGCACGGGCAAGACCCACCTGGCGCTCGCCATCACTGCCGCAGTGGTACGCGCCGGCACCAG
AGGCCGATACTTCAATACGGTTGATCTGGTGAACCGGCTTGAGGAAGAAGCACGACTGGGCAAAGCCGGAAGCCTTGCCGCTCAACTCTCACGGCTTGAT
CTCGTTGTGCTCGATGAACTGGGCTATCTCCCGTTCGCCCGTTCCGGCGGCCAGATGCTCTTCCACCTGATCAGCAAGCTCTACGAAAAGACCTCGGTGA
TCATCACGACCAATCTCGCCTTTGGCGAATGGCCGAGCGTCTTCCACGACGCCAAGATGACCACCGCGCTCCTTGATCGCATCACCCACCATTGCGACAT
CATCGAGACAGGCAACGACAGCTGGCGGTTCAAAAACCGAAACTGAGCCCAAAACCCCTTCCCCCGGACCCCCATCCCCGGCGGTTAACCCGGTGGGTCA
ACAGGGACCTCCCACGGACGCCGCCGCCAGCACACCTGACGGTGCGCGTCGTCGTCCGTGGGCCCCTCCTATCGACTACCGGGATAACCGCCGCGCTGCC
GAAAGGTGGGGTCAATATTGGGCGCCGATAGGGGGTCAGTTTTGCGCGCCGGTTGACA
Protein section
ORF number : 2
ORF 1
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
1515 bp | 504 aa | 117 | 1631 | + | No |
Chemistry : DDE
ORF sequence :
MLVVETIARIRREHRDGKPIKAIARDLRLSRNTVRKAVRAPDADASYERKEQHRPRTGPFSARLEELLEQNEGLPRRDRLRMTRIHDLLQREGFGGSYDA
VRRYAARWKRERRADAGDMGKVFIPLMYRPGEAYQFDWSHEDVEIAGKPMRVKVAHMRLCWSRAPFVRAYPRETQEMVFDAHSRAFAFFGGVPTRGIYDN
MKTAVTTVFTGKERLFNRRFLIMTDHYRVEPVACSPAAGWEKGQVENQVQTSRERLFKPRLRFASLEELNIWLEAECRRWAERNQHPDQADMTIAQALEA
ELPMLQPLPVPFDGFFESEHVASSTCLVSFDRNRYSVMAKAAHQAVQVRAYATRIVIRCDGAVVAEHERVFGRNQTIFDPWHYLPILARKPGALRNGAPF
QDWALPPALAQLRRKLGKGDDADRRFVRVLAAIPDDGLEVVEAAVAEALSAGTVSDEVILNILSRRREPRDAQTMDVVVNLKLKHPPIADCARYDTVRGL
NAAA
VRRYAARWKRERRADAGDMGKVFIPLMYRPGEAYQFDWSHEDVEIAGKPMRVKVAHMRLCWSRAPFVRAYPRETQEMVFDAHSRAFAFFGGVPTRGIYDN
MKTAVTTVFTGKERLFNRRFLIMTDHYRVEPVACSPAAGWEKGQVENQVQTSRERLFKPRLRFASLEELNIWLEAECRRWAERNQHPDQADMTIAQALEA
ELPMLQPLPVPFDGFFESEHVASSTCLVSFDRNRYSVMAKAAHQAVQVRAYATRIVIRCDGAVVAEHERVFGRNQTIFDPWHYLPILARKPGALRNGAPF
QDWALPPALAQLRRKLGKGDDADRRFVRVLAAIPDDGLEVVEAAVAEALSAGTVSDEVILNILSRRREPRDAQTMDVVVNLKLKHPPIADCARYDTVRGL
NAAA
Blast result :ORF 2
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
711 bp | 236 aa | 1636 | 2346 | + | No |
AG : IS21 helper
ORF sequence :
MAALKGLGLKGMVTAFDDAVTNGIRRDRTIMEMLADLLRAETAHREAASIRYRMTAAKLPAIKDLDGFMFDGTPINEGLIRSLHAGSFLPERRNIVLVGG
TGTGKTHLALAITAAVVRAGTRGRYFNTVDLVNRLEEEARLGKAGSLAAQLSRLDLVVLDELGYLPFARSGGQMLFHLISKLYEKTSVIITTNLAFGEWP
SVFHDAKMTTALLDRITHHCDIIETGNDSWRFKNRN
TGTGKTHLALAITAAVVRAGTRGRYFNTVDLVNRLEEEARLGKAGSLAAQLSRLDLVVLDELGYLPFARSGGQMLFHLISKLYEKTSVIITTNLAFGEWP
SVFHDAKMTTALLDRITHHCDIIETGNDSWRFKNRN
Blast result :
Comments
ISSsp4 is 65%(istA) and 74%(istB) aa similar to ISGur15.
L1 : AAAATTGACCCCCTT
L2 : AAAATTGACCCCCTT
R1 : AAAACTGACCCCCTA
L1 : AAAATTGACCCCCTT
L2 : AAAATTGACCCCCTT
R1 : AAAACTGACCCCCTA
References
1] Habe,H., Ashikawa,Y., Saiki,Y., Yoshida,T., Nojiri,H. and Omori,T.(2002) FEMS Microbiol. Lett. 211 (1), 43-49.
2] Inoue,K., Widada,J., Nakai,S., Endoh,T., Urata,M., Ashikawa,Y., Shintani,M., Saiki,Y., Yoshida,T., Habe,H., Omori,T. and Nojiri,H.(2004)Biosci. Biotechnol. Biochem. 68 (7), 1467-1480.
3] Inoue,K., Habe,H., Yamane,H., Omori,T. and Nojiri,H.(2005)FEMS Microbiol. Lett. 245 (1), 145-153.
4] Urata,M., Uchimura,H., Noguchi,H., Sakaguchi,T., Takemura,T., Eto,K., Habe,H., Omori,T., Yamane,H. and Nojiri,H.(2006)Appl. Environ. Microbiol. 72 (5), 3198-3205.
5] Shintani,M., Urata,M., Inoue,K., Eto,K., Habe,H., Omori,T., Yamane,H. and Nojiri,H.(2007)J. Bacteriol. 189 (5), 2007-2020.
2] Inoue,K., Widada,J., Nakai,S., Endoh,T., Urata,M., Ashikawa,Y., Shintani,M., Saiki,Y., Yoshida,T., Habe,H., Omori,T. and Nojiri,H.(2004)Biosci. Biotechnol. Biochem. 68 (7), 1467-1480.
3] Inoue,K., Habe,H., Yamane,H., Omori,T. and Nojiri,H.(2005)FEMS Microbiol. Lett. 245 (1), 145-153.
4] Urata,M., Uchimura,H., Noguchi,H., Sakaguchi,T., Takemura,T., Eto,K., Habe,H., Omori,T., Yamane,H. and Nojiri,H.(2006)Appl. Environ. Microbiol. 72 (5), 3198-3205.
5] Shintani,M., Urata,M., Inoue,K., Eto,K., Habe,H., Omori,T., Yamane,H. and Nojiri,H.(2007)J. Bacteriol. 189 (5), 2007-2020.