ISSsp4

  • Family IS21
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
AB270530 ND Sphingomonas sp.
Sphingomonas sp. KA1 plasmid pCAR3
DNA section
IS Length : 2558 bp

Ends


IR Length : 24/29

IRL : TGTGGAGCGGCGTGCAAAATTGACCCCCTTAGCGGGGTGATCGGCGTCTA
IRR : TGTCAACCGGCGCGCAAAACTGACCCCCTATCGGCGCCCAATATTGACCC

Insertion site


Left flankDirect repeatRight flankDR Length
GCCACTACAACCTACAACAC0

DNA sequence

TGTGGAGCGGCGTGCAAAATTGACCCCCTTAGCGGGGTGATCGGCGTCTAAAATTGACCCCCTTCATCTCATCATGAGAAGCGCCGCCGTTTGGGTTCCG
GACGGCGGGTACAGGGATGTTGGTTGTGGAGACGATTGCGAGGATCCGTCGGGAGCACCGGGACGGGAAGCCTATCAAGGCGATAGCGCGGGATTTGCGG
CTATCGCGCAACACGGTGCGCAAGGCGGTCCGGGCACCGGACGCGGACGCCAGTTACGAGCGTAAGGAACAGCACCGGCCGCGGACCGGACCTTTCAGTG
CTCGTCTTGAAGAATTGCTCGAGCAGAATGAAGGGCTGCCCCGGCGTGACCGGCTTCGTATGACGAGGATCCATGATCTGCTTCAGCGCGAAGGGTTCGG
CGGCTCCTATGACGCTGTGCGGCGCTATGCCGCGCGCTGGAAGCGTGAGCGTCGCGCCGACGCCGGCGATATGGGGAAGGTGTTCATCCCATTGATGTAC
AGGCCAGGCGAGGCTTACCAGTTCGACTGGAGCCACGAGGATGTCGAGATCGCGGGCAAGCCGATGCGGGTGAAGGTCGCTCATATGCGATTATGCTGGT
CACGGGCACCATTCGTCCGTGCATATCCTCGCGAGACGCAGGAGATGGTGTTCGACGCACATTCCCGCGCCTTTGCGTTCTTCGGCGGGGTTCCGACGCG
CGGCATCTACGACAATATGAAGACGGCTGTGACGACGGTGTTCACCGGCAAGGAACGCCTGTTCAACCGGCGGTTCCTGATCATGACGGACCACTATCGC
GTCGAGCCGGTGGCCTGCAGTCCTGCCGCAGGGTGGGAGAAGGGGCAGGTCGAGAACCAGGTTCAGACTAGCAGGGAGCGCCTGTTCAAGCCGCGCCTGC
GCTTTGCCAGCCTCGAGGAGCTGAACATCTGGCTGGAGGCCGAGTGCCGTCGCTGGGCGGAACGCAACCAGCATCCTGACCAGGCAGATATGACGATCGC
GCAGGCGCTGGAGGCGGAGCTTCCCATGCTGCAACCGCTACCGGTCCCCTTCGACGGGTTCTTCGAGAGCGAACACGTAGCCAGTTCGACGTGCCTCGTG
AGCTTCGATCGCAATCGGTATTCGGTGATGGCAAAGGCGGCCCATCAAGCTGTGCAGGTGCGCGCCTACGCAACGCGCATCGTGATCCGCTGCGACGGCG
CTGTCGTTGCCGAACACGAACGGGTCTTCGGTCGGAACCAGACGATCTTCGATCCGTGGCATTACCTGCCCATCCTCGCCCGCAAGCCCGGTGCGTTGCG
CAATGGCGCCCCCTTCCAGGACTGGGCTCTCCCACCTGCACTGGCCCAACTGCGGCGCAAGCTGGGCAAGGGCGATGATGCGGATCGCCGCTTCGTCCGC
GTCCTGGCCGCCATTCCCGACGACGGCCTCGAGGTTGTTGAAGCCGCAGTCGCCGAGGCCCTCAGCGCAGGAACGGTCAGCGACGAGGTCATCCTCAACA
TCCTGTCCCGCCGGCGTGAGCCTCGCGATGCCCAGACCATGGACGTCGTCGTCAACCTGAAGCTCAAGCATCCGCCGATTGCAGACTGCGCCCGCTACGA
CACGGTGCGAGGCCTCAATGCAGCGGCATGAGATTGTGGCAGCCCTCAAGGGGCTGGGGCTCAAGGGCATGGTCACTGCCTTCGATGATGCCGTCACCAA
TGGCATCCGCCGCGACAGAACGATCATGGAGATGCTGGCCGACCTGCTGCGTGCCGAGACTGCACATCGGGAGGCAGCGTCGATCCGCTACCGGATGACC
GCGGCGAAGCTTCCGGCAATCAAGGACCTGGATGGGTTCATGTTCGACGGAACGCCGATCAACGAAGGGCTGATCCGCTCCCTGCACGCCGGCTCCTTCC
TGCCGGAACGGCGCAATATCGTGCTGGTAGGTGGAACGGGCACGGGCAAGACCCACCTGGCGCTCGCCATCACTGCCGCAGTGGTACGCGCCGGCACCAG
AGGCCGATACTTCAATACGGTTGATCTGGTGAACCGGCTTGAGGAAGAAGCACGACTGGGCAAAGCCGGAAGCCTTGCCGCTCAACTCTCACGGCTTGAT
CTCGTTGTGCTCGATGAACTGGGCTATCTCCCGTTCGCCCGTTCCGGCGGCCAGATGCTCTTCCACCTGATCAGCAAGCTCTACGAAAAGACCTCGGTGA
TCATCACGACCAATCTCGCCTTTGGCGAATGGCCGAGCGTCTTCCACGACGCCAAGATGACCACCGCGCTCCTTGATCGCATCACCCACCATTGCGACAT
CATCGAGACAGGCAACGACAGCTGGCGGTTCAAAAACCGAAACTGAGCCCAAAACCCCTTCCCCCGGACCCCCATCCCCGGCGGTTAACCCGGTGGGTCA
ACAGGGACCTCCCACGGACGCCGCCGCCAGCACACCTGACGGTGCGCGTCGTCGTCCGTGGGCCCCTCCTATCGACTACCGGGATAACCGCCGCGCTGCC
GAAAGGTGGGGTCAATATTGGGCGCCGATAGGGGGTCAGTTTTGCGCGCCGGTTGACA
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
1515 bp504 aa1171631+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MLVVETIARIRREHRDGKPIKAIARDLRLSRNTVRKAVRAPDADASYERKEQHRPRTGPFSARLEELLEQNEGLPRRDRLRMTRIHDLLQREGFGGSYDA
VRRYAARWKRERRADAGDMGKVFIPLMYRPGEAYQFDWSHEDVEIAGKPMRVKVAHMRLCWSRAPFVRAYPRETQEMVFDAHSRAFAFFGGVPTRGIYDN
MKTAVTTVFTGKERLFNRRFLIMTDHYRVEPVACSPAAGWEKGQVENQVQTSRERLFKPRLRFASLEELNIWLEAECRRWAERNQHPDQADMTIAQALEA
ELPMLQPLPVPFDGFFESEHVASSTCLVSFDRNRYSVMAKAAHQAVQVRAYATRIVIRCDGAVVAEHERVFGRNQTIFDPWHYLPILARKPGALRNGAPF
QDWALPPALAQLRRKLGKGDDADRRFVRVLAAIPDDGLEVVEAAVAEALSAGTVSDEVILNILSRRREPRDAQTMDVVVNLKLKHPPIADCARYDTVRGL
NAAA

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
711 bp236 aa16362346+No
ORF function : Accessory Gene
AG : IS21 helper

ORF sequence :

MAALKGLGLKGMVTAFDDAVTNGIRRDRTIMEMLADLLRAETAHREAASIRYRMTAAKLPAIKDLDGFMFDGTPINEGLIRSLHAGSFLPERRNIVLVGG
TGTGKTHLALAITAAVVRAGTRGRYFNTVDLVNRLEEEARLGKAGSLAAQLSRLDLVVLDELGYLPFARSGGQMLFHLISKLYEKTSVIITTNLAFGEWP
SVFHDAKMTTALLDRITHHCDIIETGNDSWRFKNRN

 

Blast result :
Comments
ISSsp4 is 65%(istA) and 74%(istB) aa similar to ISGur15.
L1 : AAAATTGACCCCCTT
L2 : AAAATTGACCCCCTT
R1 : AAAACTGACCCCCTA
References
1] Habe,H., Ashikawa,Y., Saiki,Y., Yoshida,T., Nojiri,H. and Omori,T.(2002) FEMS Microbiol. Lett. 211 (1), 43-49.

2] Inoue,K., Widada,J., Nakai,S., Endoh,T., Urata,M., Ashikawa,Y., Shintani,M., Saiki,Y., Yoshida,T., Habe,H., Omori,T. and Nojiri,H.(2004)Biosci. Biotechnol. Biochem. 68 (7), 1467-1480.

3] Inoue,K., Habe,H., Yamane,H., Omori,T. and Nojiri,H.(2005)FEMS Microbiol. Lett. 245 (1), 145-153.

4] Urata,M., Uchimura,H., Noguchi,H., Sakaguchi,T., Takemura,T., Eto,K., Habe,H., Omori,T., Yamane,H. and Nojiri,H.(2006)Appl. Environ. Microbiol. 72 (5), 3198-3205.

5] Shintani,M., Urata,M., Inoue,K., Eto,K., Habe,H., Omori,T., Yamane,H. and Nojiri,H.(2007)J. Bacteriol. 189 (5), 2007-2020.