ISfinder Mirror Site

ISTesp3

Family IS3
Group IS51

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
AP008980	ND	Terrabacter sp.	Terrabacter sp. DBF63

DNA section

IS Length : 1373 bp

Ends

IR Length : 25/30

IRL : TCGGGGCCTGACCCCGGTTCTTGGACACGCTGAATCCAGCACGTGGTGCT
IRR : CGGGCCTTACCCCCGGATCTTGGACACGGGGTGTGATTACGCGGCGGTTG

Insertion site

Left flank	Direct repeat	Right flank	DR Length
GGCCGTGAGATAGCGG	CTG	GTTCCAAGTAACCGA	3

DNA sequence

TCGGGGCCTGACCCCGGTTCTTGGACACGCTGAATCCAGCACGTGGTGCTGGGGAAGCGAGATGATCCGAGAATCATGGCTAGGAAGAACTATTCCGAGG
AGTTCCGTCGTCAGGCCGTCGACTTGTACGAGTCCACCCCGGGTGCCACGGTCCGCGGCATCGCCGAGGACCTCGGCATCGTGCGTGGCACGCTGCGGGG
TTGGCTCGAGGTCTACGGGACCGGCAAGAAGACCGCTGCTGACGGGACGTTGACCTCCAGCCCACTGCAGTCCAGGCCGTCGAGGAAGAACTCCTCGGCG
CCCGAAGGTGAGACGCCGGAGCAGAAGATCGACCGACTGGAGGCCGAGAACGCGGCGCTGCGGGCCGAGACCACCAAGCTCACCACCGAGCGGGAGATCC
TGCAGCGGGCGGCCAAGTATTTCGCCGGGGAGACGCGCTGGTGAGTCGCTTCCAGTTCGTCGCCGACAACTCCGCCACCTTCGAGGTGAAGCGACTGTGC
GAGCTCGTCGAGATCGAGCGCTCCTCCTACTACGCCTGGCTCAAGGCAGCCCCGGCACGCGAAGAGCGAGCTCGGGCCGATGCCGAGCTCGCGGAGCGGA
TCAGGGCCGTCCACGCCGAGGACAACACTTGCGGTGCGCCGCGGATCACCGCCGAGCTCAACGACAACACCCCTGCCGGTGAGCGGGTCAACCACAAGCG
CGTCGCGCGGGTGATGCGGGCCGAGGGCATCCGTGGCTATGTGAAGAGGCGGCGGGTGCGGACCACGATCCCCGAGCCGTCGGGGCAGAAGTACCCCGAC
CTGCTCCAGCGCGACTTCACCGCCGAGCGCCCCAACCGGCGCTACGTCGGCGACATCACCTACCTCCCGCTCGCGGACGGGACGAACCTGTACCTGGCGA
CCGTGATCGACTGCTACTCCCGACGGCTCGCCGGCTGGGCGATCGCCGACCACATGCGCACCGAACTCGTCGAGGAAGCGTTGAACGTCGCGGCCGCGAC
CCGAGACAGCCTCAAGGGCGCGATCTTCCACAGCGACCACGGGTCGGTCTACTGCTCCAAGGCCTACGCCCAGCTCTGCAAGAAGTTCGGCGTCACCCAG
TCGATGGGCGCCGTCGGCTCGTCGGCCGACAACGCCCTCGCGGAGTCGTTCAACGCCACGATGAAGCGCGAGGTCCTCCAAGACACCGCCTGCTGGCCCG
ACGAGCTGACCTGCCGCCGGCAGGTCTTCAGATGGCTCGTCCGCTACAACACCCGCCGCCGTCACAGCTGGTGCGGCTACCTCTCCCCGTCCACCTACGA
GGCCCGCCGGGCCGCTACGCTGCCAACCGCCGCGTAATCACACCCCGTGTCCAAGATCCGGGGGTAAGGCCCG

Recoding section

Recoding by frameshift
Frame
Type
Experimentally demonstrated

Stimulators :

Shine-Dalgarno sequence :
Secondary structure :

Recoding motif :

Protein section

ORF number : 3

ORF 1

Length		Begin	End	Strand	Fusion ORF
369 bp	122 aa	76	444	+	No

ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MARKNYSEEFRRQAVDLYESTPGATVRGIAEDLGIVRGTLRGWLEVYGTGKKTAADGTLTSSPLQSRPSRKNSSAPEGETPEQKIDRLEAENAALRAETT
KLTTEREILQRAAKYFAGETRW

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
1026 bp	341 aa	312	1337	+	No

ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

DAGAEDRPTGGRERGAAGRDHQAHHRAGDPAAGGQVFRRGDALVSRFQFVADNSATFEVKRLCELVEIERSSYYAWLKAAPAREERARADAELAERIRAV
HAEDNTCGAPRITAELNDNTPAGERVNHKRVARVMRAEGIRGYVKRRRVRTTIPEPSGQKYPDLLQRDFTAERPNRRYVGDITYLPLADGTNLYLATVID
CYSRRLAGWAIADHMRTELVEEALNVAAATRDSLKGAIFHSDHGSVYCSKAYAQLCKKFGVTQSMGAVGSSADNALAESFNATMKREVLQDTACWPDELT
CRRQVFRWLVRYNTRRRHSWCGYLSPSTYEARRAATLPTAA

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
1262 bp	420 aa	76	1337	+	Yes

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MARKNYSEEFRRQAVDLYESTPGATVRGIAEDLGIVRGTLRGWLEVYGTGKKTAADGTLTSSPLQSRPSRKNSSAPEGETPEQKIDRLEAENAALRAETT
KLTTEREILQRAAKYFRRGDALVSRFQFVADNSATFEVKRLCELVEIERSSYYAWLKAAPAREERARADAELAERIRAVHAEDNTCGAPRITAELNDNTP
AGERVNHKRVARVMRAEGIRGYVKRRRVRTTIPEPSGQKYPDLLQRDFTAERPNRRYVGDITYLPLADGTNLYLATVIDCYSRRLAGWAIADHMRTELVE
EALNVAAATRDSLKGAIFHSDHGSVYCSKAYAQLCKKFGVTQSMGAVGSSADNALAESFNATMKREVLQDTACWPDELTCRRQVFRWLVRYNTRRRHSWC
GYLSPSTYEARRAATLPTAA

Blast result :

Comments

ISTesp3 is 68% aa similar to IS1206. The third ORF is a putative tranposase reconstructed in silico by possible -1 frameshift.

References

1] Kasuga,K., Nojiri,H., Yamane,H., Kodama,T. and Omori,T. (1997) J. Ferment. Bioeng. 84, 387-399
2] Kasuga,K., Habe,H., Chung,J.S., Yoshida,T., Nojiri,H., Yamane,H. and Omori,T. (2001) Biochem. Biophys. Res. Commun. 283 (1), 195-204
3] Habe,H., Miyakoshi,M., Chung,J., Kasuga,K., Yoshida,T., Nojiri,H. and Omori,T. (2003) Appl. Microbiol. Biotechnol. 61 (1), 44-54
4] Habe,H., Chung,J.S., Kato,H., Ayabe,Y., Kasuga,K., Yoshida,T., Nojiri,H., Yamane,H. and Omori,T. (2004) J. Bacteriol. 186 (17), 5938-5944
5] Noumura,T., Habe,H., Widada,J., Chung,J.S., Yoshida,T., Nojiri,H. and Omori,T. (2004) FEMS Microbiol. Lett. 239 (1), 147-155
6] Takagi,T., Habe,H., Yoshida,T., Yamane,H., Omori,T. and Nojiri,H. (2005) Appl. Microbiol. Biotechnol. 68 (3), 336-345
7] Habe,H., Chung,J.S., Ishida,A., Kasuga,K., Ide,K., Takemura,T., Nojiri,H., Yamane,H. and Omori,T. (2005) Microbiology (Reading, Engl.) 151 (PT 11), 3713-3722