ISfinder Mirror Site

ISTesp2

Family ISL3
Group

MGE type ISRelated element(s) :
Isoform Synonym(s) ISMlu4

Accession number	Transposition	Origin	Host
AB084235	ND	Terrabacter sp.	Terrabacter sp. DBF63 Micrococcus luteus NCTC 2665

DNA section

IS Length : 1397 bp

Ends

IR Length : 25/30

IRL : GGCTCTCCTGACGCTTCCGTGGGTGTTTTCGCGCGCCGTTGACGAGGGCA
IRR : GGCTCTCCTGACTGATCCGTGGGTCATTTCCGGCCTGGGAGGACGGGTCG

Insertion site

Left flank	Direct repeat	Right flank	DR Length
ACGGTATTGA		CTATTACAAGCTG	0
AGCTTGACGAC	ACTTTTTT	GTCGTCTGAT	8

DNA sequence

GGCTCTCCTGACGCTTCCGTGGGTGTTTTCGCGCGCCGTTGACGAGGGCACGCCGCTCTCTGCCTAGGTTTCTGGCTGTCGAGGTCAGTTACCTGAAGAG
CGGAGAACGGCGTGCGGAACGCAAGCCTATGGCGGTCCTTGCTGGGCGTCGAGAAGACGGTCGTGGAGGACATCGAGTTCGATGAGGACGACCAGCTGCT
GGTGGCTCATGTCCGTCCCCGGGCTCGGGTGCGTGGCCGGTGCGGACGGTGCGGGCGCAGGTCGCCCGGGTACGACCGTGGCGAGGGTCGGCGGCGGTGG
CGGGCCCTGGATCTGGGCACGGTGCAGGTCTACCTCGAGGCCGACGCCCCGCGGGTGGCCTGCCGCCAGCACGGCCCCACGGTGGCCGCCGTCCCGTGGG
CCAGGCACGGGGCCGGCCACACCCTGGTCTTCGACGAGCAGGTGGCCTGGCTGGCCACGCAGTGCTCCAAGTCCGCGGTCACCGAGCTGATGCGCATCGC
CTGGCGCACCGTCGGGGCGATCATCACCCGGGTCTGGACCGACGTGGAGGCCCTCGGCGACCGGTTCGCCGGCCTGCGACGGATCGGCATCGATGAGATC
TCCTACAAGAAGGGCCACCGCTACCTCACGATCGTGGTCGACCACGACACCGGCCGGCTCGTCTGGGCCGCCCCGGGCCGGGACAAGCCCACCCTGGCGA
GGTTCTTCGAGGCCCTCGGGCCCGACCGGTGCGCCGCGATCACCCACGTCAGCGCCGACGGGGCGGACTGGATCGCCGCCGTGGTCGCCCAGCGGTGCCC
GAACGCGGTGCGCTGCGCCGATCCGTTCCACATCGTCAAGTGGGCCACCGACGCCCTGGACGAGGTCCGTCGCCAGGCGTGGAACGAAGCCCGCGGCGCC
GTCCGGCAGCGCCGGGCCGGGCGGGCCAGCGGGCACGCCAAGGCGCTCAAGCACGCCCGGTACGCGCTGTGGAAGAACCCCGAGAACCTCACCACCCGGC
AGCAGGCCAAGCTCGCCTGGGTCGCCAAGACCGACCCGCGCCTGCACCGGGCCTACCTGCTCAAGGAAGGACTGCGCCTGGTCTTCCAGCTACCCGCCGA
CGAGGCCGAGACGGCCCTGGAACGCTGGATCGGCTGGGCCCGACGCTGCCGCATCCCCGCCTTCGTCGAGCTCCAACGCCGCATCGTCAAGCACAAGGCC
TCGATCCTCGCCGCCATCGAGCACGGCCTGTCCAACGGACGCATCGAATCCGTGAACACCAAGATCCGACTCATCACCCGCGTCGCCTTCGGCTTCAAGT
CCCCCGAAGCCCTCATCGCCCTCGCCATGCTCAACCTCGGCGGCCACCGACCCGTCCTCCCAGGCCGGAAATGACCCACGGATCAGTCAGGAGAGCC

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
1263 bp	420 aa	112	1374	+	No

ORF function : Transposase
Description :

ORF sequence :

MRNASLWRSLLGVEKTVVEDIEFDEDDQLLVAHVRPRARVRGRCGRCGRRSPGYDRGEGRRRWRALDLGTVQVYLEADAPRVACRQHGPTVAAVPWARHG
AGHTLVFDEQVAWLATQCSKSAVTELMRIAWRTVGAIITRVWTDVEALGDRFAGLRRIGIDEISYKKGHRYLTIVVDHDTGRLVWAAPGRDKPTLARFFE
ALGPDRCAAITHVSADGADWIAAVVAQRCPNAVRCADPFHIVKWATDALDEVRRQAWNEARGAVRQRRAGRASGHAKALKHARYALWKNPENLTTRQQAK
LAWVAKTDPRLHRAYLLKEGLRLVFQLPADEAETALERWIGWARRCRIPAFVELQRRIVKHKASILAAIEHGLSNGRIESVNTKIRLITRVAFGFKSPEA
LIALAMLNLGGHRPVLPGRK

Blast result :

Comments

ISTesp2 is 47% aa similar to ISMbu4.

References

1] Habe,H., Miyakoshi,M., Chung,J., Kasuga,K., Yoshida,T., Nojiri,H. and Omori,T. (2003) Appl. Microbiol. Biotechnol. 61 (1), 44-54
2] Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Lowry,S., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Lykidis,A., Young,M. and Greenblatt,C. (2009) Direct GenBank submission.