ISfinder Mirror Site

ISChy6

Family IS481
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_007503	ND	Carboxydothermus hydrogenoformans	Carboxydothermus hydrogenoformans Z-2901

DNA section

IS Length : 3020 bp

Ends

IR Length : 20/30

IRL : TGTTATTTAGCAAATTATCTTCTCTCAAATCCCCAGAAATTTTTCAAAAT
IRR : TGTGAACTTGCAAATTATTTGGGCACCAATATTTCAAAATAAAATAGCAT

Insertion site

Left flank	Direct repeat	Right flank	DR Length
CATATAATAT		GAATCCCTTA	0

DNA sequence

TGTTATTTAGCAAATTATCTTCTCTCAAATCCCCAGAAATTTTTCAAAATAAAACAGCGGCTTAACCTTTTTAGACCACAAATATTTTGCTATAATTTTT
GCAAAATATTTGGGGTGATTTATTTTGATGATTTTTTATGATTTTGGCTTATCTGTCCGGGAATATGCCCGGCGGGGCAAAAAGAATGATTTTCCCGATG
TTGACATTTGCCCACATTGTAAAGGGCATATTAGACTGTTACGGCATGGGTTTTATTTTAGATACGCCGTCACTAATAATAACGTTTTTTCCATCCCTAT
TTGCCGTTTAAGATGTCCTTCCTGTGGTAAAACCGTCTCAATTATCCCTTCTTTCTTACTTCCGTACTTTCAGCATACTGTGGCTACTATTTTGATAAAA
CTTAAACATTACTTCCTTTATCGAAAAGTCATAGGTTATCGTCAGTTATTGGAGTTTTTTAATCAAAGGTTTAGGAAAAATCTAACCCGTATCGAGATGT
TTTTCCGAGATGTCGGCTTTAGGGGCGTTTTTCCTTTTAACCCAAAAGAAAAAGCCATAAAGTTGGTGGAAATGCTCCTGGCTTTGGGCAAAACTCCCCT
CCTGAAAAGGGGCATCGGACATTTCCCACTTAACTTTATGGCAAGTTAATTCTATCATTTTTTCTCCCTCCTGAAAACTATTTTTTGCTTCCACAAACCT
TTTGCGTGGTATTCTTTTTCCTCCCAGGTTAAAATGGTCTCATGGATGGTTAACCGGTTAGCCTCCTTCTAAAAGGAGGGTTTTTATGGATGAAAAAGTG
CGTGAAAAGGTGGCTCTTTTCCGTTATGGCCTTCTGGCTCCTGTTCTAAATGGCCAGGTGTCTAATCAGCAGGAGTATTTTGCCCAAATTTGCTCTAAGG
TTCATGAGGTGCCTTTTTATGGCCCTAAAGAATTTACTCCTAAAACTCTGGCCGGCTGGCTTCTTTCTTACCGCCGGTTTGGTTTTGATGGCCTTAAGCC
TAAAATTCGTTCGGATCGGGGAAAACATCGAGTGCTTTCCCGGGAGCAGGAAGAGTATTTAATAGCTTTACGAAAAGAGCTCAGGGATTTGCCTGCTTCC
GTTTTTTATGAACGCTTAGTGGATAGGGGTGAGATTTTACCTAAGGATGTTTCCTATGCTACGGTTTATAGGCTACTGAAAAAGTATGGTTTGGTCGGTA
ATGAGTCCCCCAAATCCCCTGAACGCAAAAGGTTTTCTTATGATACGGTTAATGTTCTCTGGCAGGGAGATATGTCTGTTGGTCCGTATATTGTGCAAAA
TGGCAAAAAGCTAAAGACTTTTCTTTTTGCTTTTATTGATGATTGCTCAAGATTGGTTCCTTTTGCCCAGTTTTTCTTTTCAGAAAAGTTTGAGTCTCTA
CGAACTGTGTTCAAGGAAGCGTTAATTCGCCGGGGTATCCCTAAGATGGTTTATGTAGATAACGGCAAAATTTATCGCTCCGATACTTTCCAAATTGCTT
GTGCCAGTCTTGGTATTATCCTGACCCATACCCAGCCTTATGATGCTCCCAGTAAGGGAAAAATAGAACGGTTCTTTGGTACGGTTAAGACCCGCTTTTT
CCCTTTGCTGAAAGAAAAACCGGCATCTTCTTTAGAGGAGCTAAACCGCCGCTTCTGGCAGTGGTTAGAGGAAGATTACCACCGAAAAGTCCATGCCTCT
CTGGGTATGACTCCTTTAGATGTTTATTTATCTCAGGTAAGTCAGGTGCGGATGATTGATGACCCCGCTTCCCTTGACCCGCTGTTTTTGAAAAGGGAAA
TGCGTAAGGTGAAACATGATGGCACTATTTCCGTTAATAATAAGCTGTATGAAGTACCACCCCGGTTTATTGGCCAAAAAATTGAAGTCCGTTTTGATGA
AGATGTGGTTTATGTTTATGAAAATGGCATAGCTGTAGATAAAGCTATCCCGGTTAATTTCGCTGATAATGCCAAGGTAAAACGCCATCGCTCGCTTTCT
TTTCAGGCTATGCTGGCGGGAAAGGAGGAGTAATCGGTGTATCGAGCTTTTTATTCTTTGACCAAAGCCCCTTTTACTAAAGAAATCAAAACCGCTGATT
CTTTCCTGTCAACTTCTTTTTCCGAAACGATGGCCCGACTTGAGTATCTAAAAAAGACCCGTGGCATTGGCCTGATTGCTGGAGAACCAGGAGCCGGTAA
AACTTTTGCTCTTCGAGCTTTTGCTGAAAGTCTCAATCCTTCTCTGTATAAGGTGCTTTATTTCCCCCTATCCACCGGTACAGTTATGGATTTCTACCGG
GGGTTGGCTATAGCTCTGGGGGAAGAACCTAAGTTTCGCAAGGTGGATCTTTTTCACCAGATTCAAACCGCTGTCCTCAGGTTTTTTCGGGAAAGAAAGA
TTACTCCGGTTTTTATCTTAGATGAGATGCAAATGGCTAAAGACCTTTTCCTTTGCGATATAAATATCCTCTTTAACTTTAGCATGGATTCGGAAAATCC
GTTTATCTTAATTCTCTCTGGCCTTCCGCATCTCTTAGATAAGCTTTCCTTAAACCAGAATAGGCCTCTGGCCCAAAGGATTGCCATGAGCTATAAAGTT
GAGCCTTTGACCCGGGAAGAGGTAGCAGGCTACATCCAGCATCAAATGGAGCTTGCCGGAGCCAAACACAGTATATTTTCCGAACAAGCTATCGAAGCTA
TTGCTTCTTTATCCCGGGGGTGGCCCCGGTTAATCAATAAGCTTGCTACCCATTGCTTGCTTTATGGTTTCCAGGCTAAAAAGGAAATCATTGATGCTGA
GGTGGTTAGGTTAGCGGCTTTTTGGATGCTGGTTTCTAATCTTGCCCGGTCGCACTTTTGCGGCCGGTTTTCTTTTTATTTTTCTTTGCTAAAATTCTGT
GATATTTTGCAAAAGGTTTTACTGGCCTACCAAATTAATTTGCAATTTTTGCAGATTACAGCTACCTCCTATGCTATTTTATTTTGAAATATTGGTGCCC
AAATAATTTGCAAGTTCACA

Protein section

ORF number : 3

ORF 1

Length		Begin	End	Strand	Fusion ORF
525 bp	174 aa	125	649	+	No

ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MMIFYDFGLSVREYARRGKKNDFPDVDICPHCKGHIRLLRHGFYFRYAVTNNNVFSIPICRLRCPSCGKTVSIIPSFLLPYFQHTVATILIKLKHYFLYR
KVIGYRQLLEFFNQRFRKNLTRIEMFFRDVGFRGVFPFNPKEKAIKLVEMLLALGKTPLLKRGIGHFPLNFMAS

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
1248 bp	415 aa	786	2033	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MDEKVREKVALFRYGLLAPVLNGQVSNQQEYFAQICSKVHEVPFYGPKEFTPKTLAGWLLSYRRFGFDGLKPKIRSDRGKHRVLSREQEEYLIALRKELR
DLPASVFYERLVDRGEILPKDVSYATVYRLLKKYGLVGNESPKSPERKRFSYDTVNVLWQGDMSVGPYIVQNGKKLKTFLFAFIDDCSRLVPFAQFFFSE
KFESLRTVFKEALIRRGIPKMVYVDNGKIYRSDTFQIACASLGIILTHTQPYDAPSKGKIERFFGTVKTRFFPLLKEKPASSLEELNRRFWQWLEEDYHR
KVHASLGMTPLDVYLSQVSQVRMIDDPASLDPLFLKREMRKVKHDGTISVNNKLYEVPPRFIGQKIEVRFDEDVVYVYENGIAVDKAIPVNFADNAKVKR
HRSLSFQAMLAGKEE

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
951 bp	316 aa	2037	2987	+	No

ORF function : Passenger Gene
Annotation : General secretion pathway domain proteinDescription :

ORF sequence :

MYRAFYSLTKAPFTKEIKTADSFLSTSFSETMARLEYLKKTRGIGLIAGEPGAGKTFALRAFAESLNPSLYKVLYFPLSTGTVMDFYRGLAIALGEEPKF
RKVDLFHQIQTAVLRFFRERKITPVFILDEMQMAKDLFLCDINILFNFSMDSENPFILILSGLPHLLDKLSLNQNRPLAQRIAMSYKVEPLTREEVAGYI
QHQMELAGAKHSIFSEQAIEAIASLSRGWPRLINKLATHCLLYGFQAKKEIIDAEVVRLAAFWMLVSNLARSHFCGRFSFYFSLLKFCDILQKVLLAYQI
NLQFLQITATSYAILF

Blast result :

Comments

ISChy6 is 72% aa similar to ISBst1. The second ORF is the transposase, ORF1 and ORF3 are passenger genes.

References

1] Wu,M., Ren,Q., Durkin,A.S., Daugherty,S.C., Brinkac,L.M., Dodson,R.J., Madupu,R., Sullivan,S.A., Kolonay,J.F., Nelson,W.C., Tallon,L.J., Jones,K.M., Ulrich,L.E., Gonzalez,J.M., Zhulin,I.B., Robb,F.T. and Eisen,J.A. (2005) (er) PLoS Genet. 1 (5), E65.