ISRsp3

  • Family IS21
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
AB069723 ND Rhizobium sp.
Rhizobium sp. AC100 plasmid pAC200
DNA section
IS Length : 2489 bp

Ends


IR Length : 30

IRL : TGTTGAGTTGCACTGAGAAGTGACCCGGGAGGGTTATAAGTTTTCACTGA
IRR : TGTTGAGTTGCACTGAGAAGTGACCCGGGATTTTCATTGAGAAGTGACCC

Insertion site


Left flankDirect repeatRight flankDR Length
GAGGGCTCGGGGAAGGCGAAACG2

DNA sequence

TGTTGAGTTGCACTGAGAAGTGACCCGGGAGGGTTATAAGTTTTCACTGAGAATTGACCCATGTTTTCCTCGCCCCTGGCTGACAGTCAGGAGGCATCGG
AGTGATCGACATGGACCTACTCAGTGTGATCCGCCGCTGGCATTTTCGGCAAGGCATCCCCATTCGCGAGATCCGGCGGCGGACTGGATTATCGCGCAAC
ACGATCCGCAAATATCTGCGCGATGACGCGGTGGAGCCGACGTTCAAGGTTCCCAAACGGCCGAGCAAGCTTGATCAGTTTGCAGAAAAACTGACGACGT
GGCTGCGGGTGGAATCTGGCAAGTCGCGCAAACAGAAGCGAACAGCCAAGCAGCTACATGCAGATCTGGTTAAGCTGGGCTACGAGGGATCATACAGCCG
TGTTGCCGCCTTCGCCCGCTCGTGGAAGACCGAACGCCAATATGAAAAGCAAACCAGTGGGCGCGGCACATTCGTACCGCTGATCTTCCAGCCCGGTGAG
GCCTTTCAGTTCGATTGGAGTGAAGATTACGCCCTTCTGAATGGCCGGCCTACCAAGCTGCAGGTGGCCCATACCAAGCTCTCCCACAGTCGGGCGTTCA
TCACGCGAGCCTATCTGCTGCAAACGCACGAGATGTTGTTCGACGCACTGACGCAGGCCTTCCGGGTGCTGGGCGGCGTGCCTCAGCGTGGAATATTCGA
CAATATGAAGACGGCTGTAGACCGGATCGGCCAGGGCAAAGTCCGACAGGTCAATGCGCGGTTCGCTGCCATGGCCAGCCACTACCTGTTCGAAACGGAT
TTTTGCAATCCGGCCTCAGGCTGGGAGAAAGGTCAGGTCGAGAAAAACGTTCAGGATGCGCGTCGACGGTTATGGCAGCCAATGCCCAACTTCCCAGATC
TTGATGCCCTCAATGCCTGGTTGGAAGAGCGGTGCATCGCACAATGGGGAGAAATCCAGCATGGCGTCCTCCCCGGCACAGTTGCCGATGTGCACGCCGT
TGAGATTATCAGCCTGATGCCGATGGGGCGGGCCTTCGATGGCTTTGTCGAACATACCAAGCGCGTGTCGCCGACCTGCCTGATCGCGTTCGAGCGCAAT
CGCTATAGCGTTCCTGCATCCTTTGCGAACCGGCCAGTGAGCCTGCGGGTCTATCCTGATCGGTTGGTCATCGCGGCCGAGGGGCAAATCTTGTGCGAGC
ACAACCGGATTATCGACCGTTCTCACCAGAAGCCGGGGCAGACGATCTATGACTGGCGGCACTATCTGGCAGTTGTTCAGCGCAAGCCTGGCGCTTTGCG
CAATGGGGCGCCATTCCTCGAAATGCCCGAAGCCTTCCGGCAACTGCAGGGGCATCTGCTCAAGCGGCTCGGTGGTGACAGGGAGATGGTCGAAATCCTC
GCCCTTGTTCTACAGCATAACGAACAGGCTGTGCTCAGCGCCGTCGAACTGGCCCTCGAAGCTGGGGTGCCGACCAAAACCCACATCCTCAATGTTCTGC
ACCGCCTGATCGATGGCAAGGCTCCACCGGCATCCCCAATCGATGCCCCGCAGGCCCTGCGCCTCGCCCAGGAGCCTGTTGCCGATGTCGAACGCTACGA
CACGCTGAGGGCGTTACGCCATGCGTCATGATCCGGCCAGCGCCGCTGTGGTTGTCATGCTACGTGGCCTCAAAATGTATGGCATGGCGCAAGCCGCTGG
TGATCTGATCGAGCAGGGCGCGCCAGCCTTTGATGCTGCCGTTCCGATCCTCTCACAGCTTCTCAAGGCGGAGATGTCCGAGCGGGAGGTCAGGTCCATT
GCTTACCAGATCAAGGCAGCCCGGTTCCCGGCCTACAAGGATCTGGCAGGCTTCGACTTTGCCGTCAGTGAGGTCAACGAGGCGCTCGTGCGTCAACTTC
ACCGTGGTGACTTTATGGATAGCGCCGACAACGTCGTACTGATCGGCGGACCAGGCACCGGCAAAAGCCACATCGCGACGGCTTTGGGCGTCCAGGCGGT
TGAGCATCACCGCAAAAAGATCCGCTTCTTCTCCACCGTCGATCTGGTCAATGCGTTGGAGCAGGAAAAGGCCGCAAACAGAGCGGGCCAGTTGGCCGAG
CGTCTGTTGCGCCTCGACCTCGTCATCCTTGATGAGCTGGGATATCTACCCTTCAGCCCTTCAGGCGGGGCCATGCTGTTCCATCTGCTCAGCAAACTCT
ATGAGCGGACCAGCGTTGTTATCACCACCAACCTGAGCTTCAGCGAGTGGTCCAGTATCTTTGGCGATGCCAAAATGACCACCGCCCTGCTGGATCGCCT
CACGCATCATTGCCATATCCTTGAAACCGGCAATGACAGCTTCCGCTTCAGAGCCAGTTCCGCCGCCCCAAAATCCAGAAAGGACAAATCACCCGCTTGA
CCCTGCTCGTCGCAGGCAGTACATTATCCACGCCCACCCGGGTCACTTCTCAATGAAAATCCCGGGTCACTTCTCAGTGCAACTCAACA
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
1521 bp506 aa1111631+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MDLLSVIRRWHFRQGIPIREIRRRTGLSRNTIRKYLRDDAVEPTFKVPKRPSKLDQFAEKLTTWLRVESGKSRKQKRTAKQLHADLVKLGYEGSYSRVAA
FARSWKTERQYEKQTSGRGTFVPLIFQPGEAFQFDWSEDYALLNGRPTKLQVAHTKLSHSRAFITRAYLLQTHEMLFDALTQAFRVLGGVPQRGIFDNMK
TAVDRIGQGKVRQVNARFAAMASHYLFETDFCNPASGWEKGQVEKNVQDARRRLWQPMPNFPDLDALNAWLEERCIAQWGEIQHGVLPGTVADVHAVEII
SLMPMGRAFDGFVEHTKRVSPTCLIAFERNRYSVPASFANRPVSLRVYPDRLVIAAEGQILCEHNRIIDRSHQKPGQTIYDWRHYLAVVQRKPGALRNGA
PFLEMPEAFRQLQGHLLKRLGGDREMVEILALVLQHNEQAVLSAVELALEAGVPTKTHILNVLHRLIDGKAPPASPIDAPQALRLAQEPVADVERYDTLR
ALRHAS

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
780 bp259 aa16212400+No
ORF function : Accessory Gene
AG : IS21 helper

ORF sequence :

MRHDPASAAVVVMLRGLKMYGMAQAAGDLIEQGAPAFDAAVPILSQLLKAEMSEREVRSIAYQIKAARFPAYKDLAGFDFAVSEVNEALVRQLHRGDFMD
SADNVVLIGGPGTGKSHIATALGVQAVEHHRKKIRFFSTVDLVNALEQEKAANRAGQLAERLLRLDLVILDELGYLPFSPSGGAMLFHLLSKLYERTSVV
ITTNLSFSEWSSIFGDAKMTTALLDRLTHHCHILETGNDSFRFRASSAAPKSRKDKSPA

 

Blast result :
Comments
ISRsp3 is 81% (ORFA) and 90% (ORFB) aa similar to IS1600.
References
1] Hashimoto,M., Fukui,M., Hayano,K. and Hayatsu,M. (2002) Appl. Environ. Microbiol. 68 (3), 1220-1227