ISfinder Mirror Site

ISPin5

Family IS200/IS605
Group IS200

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_008709	ND	Psychromonas ingrahamii	Psychromonas ingrahamii

DNA section

IS Length : 593 bp

Ends

Left end : TAGTCTCCTGAAAAGGTTAAAACCCTCGGCTTTAGCCGGTCAGCTTTAGCTATAAAATCTTAATAGGTATTTGGTTAGGAGATGGGAATGGATTATCGAT II struct. : Yes
Right end : ATAATTTTAGAACTGAGCACTAAAACAACGGGTCTTTGACCCGTATCCGGACTTTAGTCCATAGATCCTCACCCACCTACTTTAGTAGCTGGTTGTTGAG II struct. : Yes

Insertion site

Left flank	LE cleavage site	Right flank	RE cleavage site
GTTTGGTTTAACT	TTAT	TTTTTGGAGTTTCA	tgag
AATCAGCTTCTTT	TTAT	TTTTAACTAATTCC	tgag

DNA sequence

TAGTCTCCTGAAAAGGTTAAAACCCTCGGCTTTAGCCGGTCAGCTTTAGCTATAAAATCTTAATAGGTATTTGGTTAGGAGATGGGAATGGATTATCGAT
ATGGTAGCCATACGGTTTTTAAGATTCAGTATCATTTTGTTTTTGTTACTAAATATAGGTATCAAGTTTTAAAAAGTGATGTTGGGTTGAAGGTCAGAGA
ATTAATCAGGCAAACATGTAATTCTTTTGAAATTGAGATTTTAAAAGAAGTTGTTAGCAAAGATCATGTTCATCTTTTAGTTTCAGCTCCCCCTAATATG
GCACCAAGTAAAATAATGAGAAGGGTGAAGGGGAGATCCTCATCTAAACTATTTAAATGTTTCCCCAACTTAAAAAAGCGATACTGGGGGCGTCATTTTT
GGTCTAGAGGATATTTTTATGTCACATCTGGGGGTGTTACTGAAGAAATGATTAAAGAGTATTTAGCCCATCATTTTGAGCCAAAAGTCGATGATAATTT
TAGAACTGAGCACTAAAACAACGGGTCTTTGACCCGTATCCGGACTTTAGTCCATAGATCCTCACCCACCTACTTTAGTAGCTGGTTGTTGAG

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
429 bp	142 aa	88	516	+	No

ORF function : Transposase
Chemistry : Y1

ORF sequence :

MDYRYGSHTVFKIQYHFVFVTKYRYQVLKSDVGLKVRELIRQTCNSFEIEILKEVVSKDHVHLLVSAPPNMAPSKIMRRVKGRSSSKLFKCFPNLKKRYW
GRHFWSRGYFYVTSGGVTEEMIKEYLAHHFEPKVDDNFRTEH

Blast result :

Comments

ISPin5 is 85% aa similar to ISPlu2.

References

1] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina del Rio,T., Hammon,N., Israni,S., Dalin,E., Tice,H., Pitluck,S., Thompson,L.S., Brettin,T., Bruce,D., Han,C., Tapia,R., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Ivanova,N., Staley,J. and Richardson,P. (2007) Direct submission GenBank.