ISCpe3
- Family IS256
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_008262 | ND | Clostridium perfringens | Clostridium perfringens SM101 |
DNA section
IS Length : 1503 bp
Ends
IR Length : 25/33
IRL : GAGAATGTAGGGAAATTTGTGTAAAAACAAATTTCCCTACAGTTTTTTTG
IRR : GAGAATGTAGAATAATTTGTGTAATTTAATATTCTATATCGGTTATATAA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TTTAATATTA | GGATCTAC | TGAACATACT | 8 |
| TGTTACTTCA | AGGACAGA | AAATTAATTC | 8 |
| CAAATAAAAG | GCAAAGTACT | 0 |
DNA sequence
GAGAATGTAGGGAAATTTGTGTAAAAACAAATTTCCCTACAGTTTTTTTGAATACTTAGCTGGAAAATTTGGAATAATATTCTTAATATTTTAAGGAGGA
TTTCAAATGAGTAAAGAAATTAATTATCAAGAAGAAATTAAAAATTGTAAATCAGTTGAAGATTTAGTTGGTAAGAATGGATTGATGAAAAGACTATTTA
AAGATGTTATGCAACAATTGCTAGATGCCGAGATGGAAGAACACCTAGGTAGAACTAAATATGAAAGATGTTCTGAAGAAAATTCTAAAAATTATAGAAA
TGGCACTTCAAACAAAACTGTTAGAAGTAGTTACGGAGACGTGGAATTAGATATTCCACGTGATAGAAACGGTGACTTTAACCCTGTTTCAGTAAAAAAA
TATAAAAAAGATTGCAATGAATTTGATCAGAAAATTATTGGATTATATGCCAGAGGAATGTCTACTAGAGATATTCAATCAGAAATGGAAGAATTATATG
GAATTGAACTATCTCCATCGGCTATTTCTCATATAACAGATAAAGTTCTCGTAGCTGCTGCCGAATGGCAAAACCGAATGCTTGATAAGGTATATCCAAT
TGTATATATGGATGCAATTCATTTTAAAGTTAGAGAAGATAATAAAATTGTATCTAAGGCTGCATATATATGTTTAGGAATAGACATGAATGGATATAAA
GATATTCTTGGAATATGGATTGGTGAAGCAGAGGGAGCTAAATTTTGGTTAAGTGTCTGTAATGATTTGAATAATCGTGGTGTTAAAGATATATTAATAG
CTTGCATGGATGGATTGAGAGGACTTCCAGAAGCCATAAAAGCTGTATTTCCTAATGTTTGTATACAAAATTGTATAGTTCATCAAATAAGAAACTCATT
AAAATACATATCTTACAAGGATAAAAAAGACTTTATGAAGGATTTAAAATTAGTATATACGGCTGAAACCGTGGAACTAGCGTTATCCCAGTTAGACATG
TTAAAAGAAAAATGGGATTATAAATATGCTAATGTCATAGATTCATGGTACTCTAATTGGAATAATTTAAGTACATTCTTTGATTTTTCAAGTGGTATAA
GAAAAATGATTTATACTACAAATTTGCTTGAAGGATTCAATAGACAGTTAAGAAAATTTACTAAGATAAGAACAGTATTTCCAACAGATGAATCACTAAG
GAAATCTTTATATTTAGCTACAGATCAAGTGATGAAAAAATGGACTTCACCAATAGCAAATTGGGGTCCTATTCTACTACAATTTGAAATAATCTTTGCG
GAGAGAATCGAAACTGCACTAGCAATTTAGCTTGTACATATTTGGATTTAAAGTATATATTTCAAAAATTTGCACATACTAAAATCAACATACTAACTCA
TTATTAGTATTTGAAGAAAAATATAAAAATATAACTGGAAATAAAATTTCCAGTTATATAACCGATATAGAATATTAAATTACACAAATTATTCTACATT
CTC
TTTCAAATGAGTAAAGAAATTAATTATCAAGAAGAAATTAAAAATTGTAAATCAGTTGAAGATTTAGTTGGTAAGAATGGATTGATGAAAAGACTATTTA
AAGATGTTATGCAACAATTGCTAGATGCCGAGATGGAAGAACACCTAGGTAGAACTAAATATGAAAGATGTTCTGAAGAAAATTCTAAAAATTATAGAAA
TGGCACTTCAAACAAAACTGTTAGAAGTAGTTACGGAGACGTGGAATTAGATATTCCACGTGATAGAAACGGTGACTTTAACCCTGTTTCAGTAAAAAAA
TATAAAAAAGATTGCAATGAATTTGATCAGAAAATTATTGGATTATATGCCAGAGGAATGTCTACTAGAGATATTCAATCAGAAATGGAAGAATTATATG
GAATTGAACTATCTCCATCGGCTATTTCTCATATAACAGATAAAGTTCTCGTAGCTGCTGCCGAATGGCAAAACCGAATGCTTGATAAGGTATATCCAAT
TGTATATATGGATGCAATTCATTTTAAAGTTAGAGAAGATAATAAAATTGTATCTAAGGCTGCATATATATGTTTAGGAATAGACATGAATGGATATAAA
GATATTCTTGGAATATGGATTGGTGAAGCAGAGGGAGCTAAATTTTGGTTAAGTGTCTGTAATGATTTGAATAATCGTGGTGTTAAAGATATATTAATAG
CTTGCATGGATGGATTGAGAGGACTTCCAGAAGCCATAAAAGCTGTATTTCCTAATGTTTGTATACAAAATTGTATAGTTCATCAAATAAGAAACTCATT
AAAATACATATCTTACAAGGATAAAAAAGACTTTATGAAGGATTTAAAATTAGTATATACGGCTGAAACCGTGGAACTAGCGTTATCCCAGTTAGACATG
TTAAAAGAAAAATGGGATTATAAATATGCTAATGTCATAGATTCATGGTACTCTAATTGGAATAATTTAAGTACATTCTTTGATTTTTCAAGTGGTATAA
GAAAAATGATTTATACTACAAATTTGCTTGAAGGATTCAATAGACAGTTAAGAAAATTTACTAAGATAAGAACAGTATTTCCAACAGATGAATCACTAAG
GAAATCTTTATATTTAGCTACAGATCAAGTGATGAAAAAATGGACTTCACCAATAGCAAATTGGGGTCCTATTCTACTACAATTTGAAATAATCTTTGCG
GAGAGAATCGAAACTGCACTAGCAATTTAGCTTGTACATATTTGGATTTAAAGTATATATTTCAAAAATTTGCACATACTAAAATCAACATACTAACTCA
TTATTAGTATTTGAAGAAAAATATAAAAATATAACTGGAAATAAAATTTCCAGTTATATAACCGATATAGAATATTAAATTACACAAATTATTCTACATT
CTC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1224 bp | 407 aa | 107 | 1330 | + | No |
Chemistry : DDE
ORF sequence :
MSKEINYQEEIKNCKSVEDLVGKNGLMKRLFKDVMQQLLDAEMEEHLGRTKYERCSEENSKNYRNGTSNKTVRSSYGDVELDIPRDRNGDFNPVSVKKYK
KDCNEFDQKIIGLYARGMSTRDIQSEMEELYGIELSPSAISHITDKVLVAAAEWQNRMLDKVYPIVYMDAIHFKVREDNKIVSKAAYICLGIDMNGYKDI
LGIWIGEAEGAKFWLSVCNDLNNRGVKDILIACMDGLRGLPEAIKAVFPNVCIQNCIVHQIRNSLKYISYKDKKDFMKDLKLVYTAETVELALSQLDMLK
EKWDYKYANVIDSWYSNWNNLSTFFDFSSGIRKMIYTTNLLEGFNRQLRKFTKIRTVFPTDESLRKSLYLATDQVMKKWTSPIANWGPILLQFEIIFAER
IETALAI
KDCNEFDQKIIGLYARGMSTRDIQSEMEELYGIELSPSAISHITDKVLVAAAEWQNRMLDKVYPIVYMDAIHFKVREDNKIVSKAAYICLGIDMNGYKDI
LGIWIGEAEGAKFWLSVCNDLNNRGVKDILIACMDGLRGLPEAIKAVFPNVCIQNCIVHQIRNSLKYISYKDKKDFMKDLKLVYTAETVELALSQLDMLK
EKWDYKYANVIDSWYSNWNNLSTFFDFSSGIRKMIYTTNLLEGFNRQLRKFTKIRTVFPTDESLRKSLYLATDQVMKKWTSPIANWGPILLQFEIIFAER
IETALAI
Blast result :
Comments
ISCpe3 is 70% aa similar to ISSod18.There are 3 copies of ISCpe3 in this strain
References
1] Myers,G.S., Rasko,D.A., Cheung,J.K., Ravel,J., Seshadri,R., DeBoy,R.T., Ren,Q., Varga,J., Awad,M.M., Brinkac,L.M., Daugherty,S.C., Haft,D.H., Dodson,R.J., Madupu,R., Nelson,W.C., Rosovitz,M.J., Sullivan,S.A., Khouri,H., Dimitrov,G.I., Watkins,K.L., Mulligan,S., Benton,J., Radune,D., Fisher,D.J., Atkins,H.S., Hiscox,T., Jost,B.H., Billington,S.J., Songer,J.G., McClane,B.A., Titball,R.W., Rood,J.I., Melville,S.B. and Paulsen,I.T. (2006) Genome Res. 16 (8), 1031-1040.