ISShes2
- Family IS3
- Group IS3
Isoform Synonym(s)
Accession number | Transposition | Origin | Host |
---|---|---|---|
NC_008321 | ND | Shewanella sp. | Shewanella sp. MR-4 |
DNA section
IS Length : 1299 bp
Ends
IR Length : 20/25
IRL : TGTCTAGTCCTGAAATACGTTGACATAAAGAGGACTTCTTTATGACAACA
IRR : TGTCCCGTCCTAAGATAGGTTGACAGTTTTTAGGCCAGCTCCAGTAGCTG
Insertion site
Left flank | Direct repeat | Right flank | DR Length |
---|---|---|---|
TTTTATTGCT | CTC | ATTTCATTTC | 3 |
TTTCGTTTTC | AAC | CTATGGGTTA | 3 |
TTTTTAGTTG | TGC | GATTTAAGAC | 3 |
TGTCGTTACT | CTC | GAAGCCAAAA | 3 |
TTTGTGTTAG | TAA | ACCTCATAAA | 3 |
TTTCTAAAAC | GTT | TAATGTCGGA | 3 |
TTGCATTGAT | GGG | GGATAAAGAC | 3 |
DNA sequence
TGTCTAGTCCTGAAATACGTTGACATAAAGAGGACTTCTTTATGACAACATCAATTAACTCAAGCCGTAAGCGCACACAACGAGATTACACCTTAGCCTT
TAAATTAGGCGTTGTAGAGCGTGTTGAAAAAGGCGAAATGACGTACAAACAAGCCCAAGCCCATTTTGGTATTCAAGGCCGTTCAACGGTACTCGTTTGG
CTTAGAAAACATGGTAGACTCGATTGGTCGAAACCTTTTCAGCATCCCCTTATGCCTAAGTCAAAAGAAACCCCTGCCGAAACTATCAAGCGCCTTGAAC
GTGAGTTAGCAGAAGAGAAACTGCGTAACAAAATCCTCAATGGTATGGTTGATATCATGGACAATGAATATGGAGCAGGCCTCAGAAAAAAGTACTTATC
CGGTACGTCTGGCAAGCAAAAAGCAACGGCGAAATAAACCTCGCTGCCGCATGTCGTGCTGCGGGTATGTCGAGGCAAGGTATTTATCAGGCAGTTGCTC
GAATGGAAAGTCGTAGAGCTGAGCTATCGGTCATCAAAGACTGGGTCCAGTACTGGCGTAAATATATGCCAAGGTTAGGGGCCCGTAAGCTCTACACGTT
GATAAAATCCAAGCTGGTTGAGCACGATATTAAACTCGGGCGAGATGGGTTCTTTACCTATTTGAGAAGTGAAGGTTTACTGGTTAAACCGAAGAAAAGC
TACACAAAAACCACGTTCAGCAAACACTGGATGAAGAAGCATCCTAATTTGCTGAAAGAGGAAGGACTGCATGATGCAGGGCATGTACTGGTCAGTGATA
TCACCTATCTTGAGTCAGACCAAGGTGTGCACTACCTGTCACTGGTCACCGATGCCAGTTCTCGTAAGATAGTCGGTCATCACGTGAGTAAAGACATGAA
AGCCGAGAGTGTGGTGAAAGCGTTAAAAATGGCCATAAAAGATAAACGCTATATCGGCAACGCAGTGCATCACTCAGACAGAGGTTTACAATACTGCTCA
GCAGTTTATCAGAATGCGCTTCAGGCGAGCCAAATCCAGCCGTCAATGACGGATGGTTATGATTGCTACCAAAATGCATTGGCAGAAAGAGTGAATGGCA
TACTGAAGCAGGAGTTTTTACTGCACCGATGTAGGACATTTGCGGAGCTGAAGATACTTGTTAGAGAATCGATAGCAATATACAACGATATGAGACCGCA
TCTGAGTTTGAATATGGCAACCCCTAATCAGGTGCACAATAGAAAAGGCCAGCTACTGGAGCTGGCCTAAAAACTGTCAACCTATCTTAGGACGGGACA
TAAATTAGGCGTTGTAGAGCGTGTTGAAAAAGGCGAAATGACGTACAAACAAGCCCAAGCCCATTTTGGTATTCAAGGCCGTTCAACGGTACTCGTTTGG
CTTAGAAAACATGGTAGACTCGATTGGTCGAAACCTTTTCAGCATCCCCTTATGCCTAAGTCAAAAGAAACCCCTGCCGAAACTATCAAGCGCCTTGAAC
GTGAGTTAGCAGAAGAGAAACTGCGTAACAAAATCCTCAATGGTATGGTTGATATCATGGACAATGAATATGGAGCAGGCCTCAGAAAAAAGTACTTATC
CGGTACGTCTGGCAAGCAAAAAGCAACGGCGAAATAAACCTCGCTGCCGCATGTCGTGCTGCGGGTATGTCGAGGCAAGGTATTTATCAGGCAGTTGCTC
GAATGGAAAGTCGTAGAGCTGAGCTATCGGTCATCAAAGACTGGGTCCAGTACTGGCGTAAATATATGCCAAGGTTAGGGGCCCGTAAGCTCTACACGTT
GATAAAATCCAAGCTGGTTGAGCACGATATTAAACTCGGGCGAGATGGGTTCTTTACCTATTTGAGAAGTGAAGGTTTACTGGTTAAACCGAAGAAAAGC
TACACAAAAACCACGTTCAGCAAACACTGGATGAAGAAGCATCCTAATTTGCTGAAAGAGGAAGGACTGCATGATGCAGGGCATGTACTGGTCAGTGATA
TCACCTATCTTGAGTCAGACCAAGGTGTGCACTACCTGTCACTGGTCACCGATGCCAGTTCTCGTAAGATAGTCGGTCATCACGTGAGTAAAGACATGAA
AGCCGAGAGTGTGGTGAAAGCGTTAAAAATGGCCATAAAAGATAAACGCTATATCGGCAACGCAGTGCATCACTCAGACAGAGGTTTACAATACTGCTCA
GCAGTTTATCAGAATGCGCTTCAGGCGAGCCAAATCCAGCCGTCAATGACGGATGGTTATGATTGCTACCAAAATGCATTGGCAGAAAGAGTGAATGGCA
TACTGAAGCAGGAGTTTTTACTGCACCGATGTAGGACATTTGCGGAGCTGAAGATACTTGTTAGAGAATCGATAGCAATATACAACGATATGAGACCGCA
TCTGAGTTTGAATATGGCAACCCCTAATCAGGTGCACAATAGAAAAGGCCAGCTACTGGAGCTGGCCTAAAAACTGTCAACCTATCTTAGGACGGGACA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
396 bp | 131 aa | 42 | 437 | + | No |
Description : First part of the transposase
ORF sequence :
MTTSINSSRKRTQRDYTLAFKLGVVERVEKGEMTYKQAQAHFGIQGRSTVLVWLRKHGRLDWSKPFQHPLMPKSKETPAETIKRLERELAEEKLRNKILN
GMVDIMDNEYGAGLRKKYLSGTSGKQKTTAK
GMVDIMDNEYGAGLRKKYLSGTSGKQKTTAK
Blast result :ORF 2
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
903 bp | 300 aa | 368 | 1270 | + | No |
Description : Second part of the transposase
ORF sequence :
IWSRPQKKVLIRYVWQAKSNGEINLAAACRAAGMSRQGIYQAVARMESRRAELSVIKDWVQYWRKYMPRLGARKLYTLIKSKLVEHDIKLGRDGFFTYLR
SEGLLVKPKKSYTKTTFSKHWMKKHPNLLKEEGLHDAGHVLVSDITYLESDQGVHYLSLVTDASSRKIVGHHVSKDMKAESVVKALKMAIKDKRYIGNAV
HHSDRGLQYCSAVYQNALQASQIQPSMTDGYDCYQNALAERVNGILKQEFLLHRCRTFAELKILVRESIAIYNDMRPHLSLNMATPNQVHNRKGQLLELA
SEGLLVKPKKSYTKTTFSKHWMKKHPNLLKEEGLHDAGHVLVSDITYLESDQGVHYLSLVTDASSRKIVGHHVSKDMKAESVVKALKMAIKDKRYIGNAV
HHSDRGLQYCSAVYQNALQASQIQPSMTDGYDCYQNALAERVNGILKQEFLLHRCRTFAELKILVRESIAIYNDMRPHLSLNMATPNQVHNRKGQLLELA
Blast result :ORF 3
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
1229 bp | 409 aa | 42 | 1270 | + | Yes |
Chemistry : DDE
ORF sequence :
MTTSINSSRKRTQRDYTLAFKLGVVERVEKGEMTYKQAQAHFGIQGRSTVLVWLRKHGRLDWSKPFQHPLMPKSKETPAETIKRLERELAEEKLRNKILN
GMVDIMDNEYGAGLRKKVLIRYVWQAKNNGEINLAAACRAAGMSRQGIYQAVARMESRRAELSVIKDWVQYWRKYMPRLGARKLYTLIKSKLVEHDIKLG
RDGFFTYLRSEGLLVKPKKSYTKTTFSKHWMKKHPNLLKEEGLHDAGHVLVSDITYLESDQGVHYLSLITDASSRKIVGHHVSKDMKAESVVKALKMAIK
DKRYIGNAVHHSDRGLQYCSAVYQNALQASQIQPSMTDGYDCYQNALAERVNGILKQEFLLHRCRTFAELKILVRESIAIYNDMRPHLSLNMATPNQVHN
RKGQLLELA
GMVDIMDNEYGAGLRKKVLIRYVWQAKNNGEINLAAACRAAGMSRQGIYQAVARMESRRAELSVIKDWVQYWRKYMPRLGARKLYTLIKSKLVEHDIKLG
RDGFFTYLRSEGLLVKPKKSYTKTTFSKHWMKKHPNLLKEEGLHDAGHVLVSDITYLESDQGVHYLSLITDASSRKIVGHHVSKDMKAESVVKALKMAIK
DKRYIGNAVHHSDRGLQYCSAVYQNALQASQIQPSMTDGYDCYQNALAERVNGILKQEFLLHRCRTFAELKILVRESIAIYNDMRPHLSLNMATPNQVHN
RKGQLLELA
Blast result :
Comments
There are 7 full length copies of ISShes2 in the Shewanela MR-4 chromosome. The third ORF is deduced from the predicted positions of programmed frameshifting and read through of in frame stop codons. The ISShes2 transposase has 57% identity to ISDde2.
References
1] Romine, M. (2008) Direct submission.