ISPre2
- Family IS5
- Group IS5
Isoform Synonym(s) ISPst11
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_004444 | ND | Pseudomonas resinovorans | Pseudomonas sp. LM8 Pseudomonas resinovorans plasmid pCAR1 Pseudomonas stutzeri 36N1 |
DNA section
IS Length : 1190 bp
Ends
IR Length : 12/18
IRL : GGAAACTCTGAAAAAGACTTTCTGATTTGGCAAAATACCGCGACCCCACC
IRR : GGATGGTCTGAAGTAGCCATGTATTTCCCCGGCATACCACCTAGCGCAAC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GCGGGCTTCA | CTAA | AGGCGGCACT | 4 |
| CACGGCTGAG | TTAG | CAAACGGCGC | 4 |
DNA sequence
GGAAACTCTGAAAAAGACTTTCTGATTTGGCAAAATACCGCGACCCCACCCACCGAGTTTCCCGATGAAGCAGATGACCTTCGCCGACGCCGAGTACGCT
GGCAAGCGCAAGCAAACCCGCAAGGAGTTGTTCCTGATCGAGATGGATCGGGTGGTGCCGTGGAAGGGCTTGATTGCTTTGATCGAGCCACATTATCCGA
AAGGTGAAGGTGGCCGTCCGGCCTACCCGTTGATGGCGATGCTGCGTGTGCATCTGCTGCAGAACTGGTTCGGCTACAGCGATCCAGCGATGGAGGAAGC
GCTGTACGAAACCACGATCCTGCGCCAGTTTGCCGGGCTGAACCTGGAGCGCATCCCCGACGAAACCACCATTCTCAACTTCCGCCGCTTGCTGGAGAAA
CACGAGCTGGCGGCCGGCATCCTCGCTGTCATCAATGGCTATCTGGGCGACCGCGGCCTGTCGCTGCGCCAGGGCACCATCGTCGATGCAACGCTGATCA
ATGCGCCCAGTTCGACCAAGAACAAGGACGGCAAGCGCGACCCGGAAATGCACCAGACCAAGAAGGGAAACCAGTATTATTTTGGCATGAAGGCCCACAT
CGGCGCCGATGACGAATCGGGTCTGGTGCACAGCGTAGTGGGCACGGCGGCCAATGTGGCGGATGTCACCCAGGTGGACAAATTGCTGCATGGCGACGAA
AACGTGGTCTGCGCCGATGCAGGCTACACCGGTGTCGAAAAGCGGCCCGAGCATGAAGGACGTGAAGTTATCTGGCAGGTGGCGGCACGCCGCAGCACCT
ACAAAAAACTCGATAAGCGCAGCGTGCTGTACAAAGCCAAGCGCAAGATTGAAAAGGCCAAGGCTCAGGTGCGCGCCAAGGTCGAGCATCCGTTCCGGGT
AATCAAGCGCCAGTTCGGTTACACCAAGGTGCGCTTTCGCGGCTTGGCCAAAAACACGGCGCAACTGGTGACACTGTTCGCTCTGTCGAACCTGTGGATG
GCGCGCCGACATTTACTGACGAATGCAGGAGAGGTGCGCCTGTAATGCGGGAAATGGCCACCGGAGTGGGGTCCGGGTGGCGAAAACGTAAGAAATACTC
GTCGAATTGATTGTTTTTTGATCATTTGGCGAGTTTAAAAGTTGCGCTAGGTGGTATGCCGGGGAAATACATGGCTACTTCAGACCATCC
GGCAAGCGCAAGCAAACCCGCAAGGAGTTGTTCCTGATCGAGATGGATCGGGTGGTGCCGTGGAAGGGCTTGATTGCTTTGATCGAGCCACATTATCCGA
AAGGTGAAGGTGGCCGTCCGGCCTACCCGTTGATGGCGATGCTGCGTGTGCATCTGCTGCAGAACTGGTTCGGCTACAGCGATCCAGCGATGGAGGAAGC
GCTGTACGAAACCACGATCCTGCGCCAGTTTGCCGGGCTGAACCTGGAGCGCATCCCCGACGAAACCACCATTCTCAACTTCCGCCGCTTGCTGGAGAAA
CACGAGCTGGCGGCCGGCATCCTCGCTGTCATCAATGGCTATCTGGGCGACCGCGGCCTGTCGCTGCGCCAGGGCACCATCGTCGATGCAACGCTGATCA
ATGCGCCCAGTTCGACCAAGAACAAGGACGGCAAGCGCGACCCGGAAATGCACCAGACCAAGAAGGGAAACCAGTATTATTTTGGCATGAAGGCCCACAT
CGGCGCCGATGACGAATCGGGTCTGGTGCACAGCGTAGTGGGCACGGCGGCCAATGTGGCGGATGTCACCCAGGTGGACAAATTGCTGCATGGCGACGAA
AACGTGGTCTGCGCCGATGCAGGCTACACCGGTGTCGAAAAGCGGCCCGAGCATGAAGGACGTGAAGTTATCTGGCAGGTGGCGGCACGCCGCAGCACCT
ACAAAAAACTCGATAAGCGCAGCGTGCTGTACAAAGCCAAGCGCAAGATTGAAAAGGCCAAGGCTCAGGTGCGCGCCAAGGTCGAGCATCCGTTCCGGGT
AATCAAGCGCCAGTTCGGTTACACCAAGGTGCGCTTTCGCGGCTTGGCCAAAAACACGGCGCAACTGGTGACACTGTTCGCTCTGTCGAACCTGTGGATG
GCGCGCCGACATTTACTGACGAATGCAGGAGAGGTGCGCCTGTAATGCGGGAAATGGCCACCGGAGTGGGGTCCGGGTGGCGAAAACGTAAGAAATACTC
GTCGAATTGATTGTTTTTTGATCATTTGGCGAGTTTAAAAGTTGCGCTAGGTGGTATGCCGGGGAAATACATGGCTACTTCAGACCATCC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 981 bp | 326 aa | 65 | 1045 | + | No |
Chemistry : DDE
ORF sequence :
MKQMTFADAEYAGKRKQTRKELFLIEMDRVVPWKGLIALIEPHYPKGEGGRPAYPLMAMLRVHLLQNWFGYSDPAMEEALYETTILRQFAGLNLERIPDE
TTILNFRRLLEKHELAAGILAVINGYLGDRGLSLRQGTIVDATLINAPSSTKNKDGKRDPEMHQTKKGNQYYFGMKAHIGADDESGLVHSVVGTAANVAD
VTQVDKLLHGDENVVCADAGYTGVEKRPEHEGREVIWQVAARRSTYKKLDKRSVLYKAKRKIEKAKAQVRAKVEHPFRVIKRQFGYTKVRFRGLAKNTAQ
LVTLFALSNLWMARRHLLTNAGEVRL
TTILNFRRLLEKHELAAGILAVINGYLGDRGLSLRQGTIVDATLINAPSSTKNKDGKRDPEMHQTKKGNQYYFGMKAHIGADDESGLVHSVVGTAANVAD
VTQVDKLLHGDENVVCADAGYTGVEKRPEHEGREVIWQVAARRSTYKKLDKRSVLYKAKRKIEKAKAQVRAKVEHPFRVIKRQFGYTKVRFRGLAKNTAQ
LVTLFALSNLWMARRHLLTNAGEVRL
Blast result :
Comments
ISPre2 is 99% aa similar to IS1384.
An isoform of ISPre2 has been identified in Pseudomonas sp. LM8 (KJ920391) by its transposition to entrapment vector pMAT1 (sacB cassette). As judged from DNA hybridization analysis, this isoform is present in 10 copies in the genome of Pseudomonas sp. LM8. Homologous sequences were also detected by DNA hybridization in Pseudomonas sp. LM5, Pseudomonas sp. LM6, Pseudomonas mendocina strain LM7, Pseudomonas aeruginosa strain LM10, Pseudomonas sp. LM11, Pseudomonas sp. LM12, Pseudomonas sp. LM13, Pseudomonas sp. LM14, Pseudomonas sp. ZM1, Pseudomonas sp. ZM2.
File updated the January 22 2015 with the information provided by Szuplewska M.
An isoform of ISPre2 has been identified in Pseudomonas sp. LM8 (KJ920391) by its transposition to entrapment vector pMAT1 (sacB cassette). As judged from DNA hybridization analysis, this isoform is present in 10 copies in the genome of Pseudomonas sp. LM8. Homologous sequences were also detected by DNA hybridization in Pseudomonas sp. LM5, Pseudomonas sp. LM6, Pseudomonas mendocina strain LM7, Pseudomonas aeruginosa strain LM10, Pseudomonas sp. LM11, Pseudomonas sp. LM12, Pseudomonas sp. LM13, Pseudomonas sp. LM14, Pseudomonas sp. ZM1, Pseudomonas sp. ZM2.
File updated the January 22 2015 with the information provided by Szuplewska M.
References
1] Urata,M., Miyakoshi,M., Kai,S., Maeda,K., Habe,H., Omori,T., Yamane,H. and Nojiri,H. (2004) J. Bacteriol. 186 (20), 6815-6823
2] Maeda,K., Nojiri,H., Shintani,M., Yoshida,T., Habe,H. and Omori,T.
(2003) J. Mol. Biol. 326 (1), 21-33
3] Szuplewska, M., and Bartosik, D. (2014) Direct submission.
2] Maeda,K., Nojiri,H., Shintani,M., Yoshida,T., Habe,H. and Omori,T.
(2003) J. Mol. Biol. 326 (1), 21-33
3] Szuplewska, M., and Bartosik, D. (2014) Direct submission.