ISJsp1

  • Family IS3
  • Group IS51
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
AB095952 ND Janthinobacterium sp.
Janthinobacterium sp. J3
DNA section
IS Length : 1312 bp

Ends


IR Length : 23/26

IRL : TGAACCGCCCCGGATTTTGTAGAGGCTCCATTGTTTGAGAGAATGGAGTC
IRR : TGAACCGCCCCGGCTTTCGTGGAGGCCGGTTAGTGTGAGTCAGGCCGGAA

Insertion site


Left flankDirect repeatRight flankDR Length
GAGGTAAATAACTGGCGCTT0

DNA sequence

TGAACCGCCCCGGATTTTGTAGAGGCTCCATTGTTTGAGAGAATGGAGTCATGAAGAAGCAACCCAAGTATTCCCCCGAAGTCATCGAGCGCGCCGTGCG
CTTGGTCAGCGAGGCCGCCAGCGAGTACAGCTCGCAGTGGGCGGCGATCGAATCCATCGCAGCCAAGATCGGCTGCACACCTGAAACGCTGCGTCGCTGG
GTACGCCAGCAAGAGCGTGATACCGGCCAGCGTCCAGGACCGACCACTGCCGAAGAAGAGCGCATCAAGACCCTGGAGCGCGAAGTGCGCGAGCTGCGCA
AAGCAAACGAGATCCTGCGTCTGGCGAGTGCGTTTTTCGCCCAGGCGGAGCTCGGCCGCCACTTCAAGCCGTGAGGGCATTTATCGACCAGTACTGCCAT
GCCTACGGTGTCGAGCCGATCTGCAAGGTGATGCAGGTCGCGCCGTCGGGCTACTGGCGTTATGCAGCGCAGCAGCGCAACCCGGCGTTGCGCTGTGCAC
GAGTCCAGCGTGACGATGTGCTGAGCGTCGACATTGAGCGGGTTTGGCAAGCAAACCTGCAGGTCTACGGCGCCGATAAAGTGTGGCGCCAACTTCGACG
TGAGGGAACTGAGGTGGCCCGCTGCACGGTGGAGCGCTTGATGCGCAAGGCCGGCCTGCGTGGCGTCATGCGTGGCAAGGTCGTACGCACGACGGCTGCC
GATACGAAGGCACCGTGCCCGCTCGACCGAGTCAACCGCCAGTTCAACGCACAGCGACCGAACCAGCTGTGGGTCAGCGATTTCACCTACGTCTCGACCT
GGCAGGGCTTCGTCTACGTGGCCTTCGTCATCGACGTCTTCGCCCGGCGCATCGTCGGCTGGCGCGTCAGCTGCTCGATGCGGACCGACTTCGTGCTCGA
CGCTTTGGAGCAGGCGCTGTACGCCCGTCAGCCCGAACGAAACGAGCTCGTCCATCATAGCGACAGGGGCTCGCAATACGTATCCATAAAGTACAGCGAG
CGTCTGGCAGAAGCCGGCATCGAGCCGTCTGTGGGCAGCAAGGGCGACAGTTACGACAATGCCCTGGCCGAGACGATCAATGGGCTCTACAAGGCTGAAC
TGATCCATCGCCGCGCTCCCTGGAAAACGCGTGAGGCCGTCGAGCTGGCCACGCTGGAATGGGTTTCCTGGTTCAACCACCATCGCCTGCTGGAACCGCT
CGGCTATATACCGCCGGCAGAAGCTGAGGCAAACTATTACAAGCAGTTGAGCAGTCAAGCCATTCCGGCCTGACTCACACTAACCGGCCTCCACGAAAGC
CGGGGCGGTTCA
Recoding section
  • Recoding by frameshift
  • Frame
  • Type
  • Experimentally demonstrated

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
324 bp107 aa51374+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MKKQPKYSPEVIERAVRLVSEAASEYSSQWAAIESIAAKIGCTPETLRRWVRQQERDTGQRPGPTTAEEERIKTLEREVRELRKANEILRLASAFFAQAE
LGRHFKP

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
1053 bp350 aa2211273+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

YRPASRTDHCRRRAHQDPGARSARAAQSKRDPASGECVFRPGGARPPLQAVRAFIDQYCHAYGVEPICKVMQVAPSGYWRYAAQQRNPALRCARVQRDDV
LSVDIERVWQANLQVYGADKVWRQLRREGTEVARCTVERLMRKAGLRGVMRGKVVRTTAADTKAPCPLDRVNRQFNAQRPNQLWVSDFTYVSTWQGFVYV
AFVIDVFARRIVGWRVSCSMRTDFVLDALEQALYARQPERNELVHHSDRGSQYVSIKYSERLAEAGIEPSVGSKGDSYDNALAETINGLYKAELIHRRAP
WKTREAVELATLEWVSWFNHHRLLEPLGYIPPAEAEANYYKQLSSQAIPA

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1223 bp407 aa511273+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MKKQPKYSPEVIERAVRLVSEAASEYSSQWAAIESIAAKIGCTPETLRRWVRQQERDTGQRPGPTTAEEERIKTLEREVRELRKANEILRLASAFFRPGG
ARPPLQAVRAFIDQYCHAYGVEPICKVMQVAPSGYWRYAAQQRNPALRCARVQRDDVLSVDIERVWQANLQVYGADKVWRQLRREGTEVARCTVERLMRK
AGLRGVMRGKVVRTTAADTKAPCPLDRVNRQFNAQRPNQLWVSDFTYVSTWQGFVYVAFVIDVFARRIVGWRVSCSMRTDFVLDALEQALYARQPERNEL
VHHSDRGSQYVSIKYSERLAEAGIEPSVGSKGDSYDNALAETINGLYKAELIHRRAPWKTREAVELATLEWVSWFNHHRLLEPLGYIPPAEAEANYYKQL
SSQAIPA

 

Blast result :
Comments
ISJsp1 is 86% aa similar to IS401. The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Inoue,K., Widada,J., Nakai,S., Endoh,T., Urata,M., Ashikawa,Y., Shintani,M., Saiki,Y., Yoshida,T., Habe,H., Omori,T. and Nojiri,H.(2004) Biosci. Biotechnol. Biochem. 68 (7), 1467-1480