ISHar2
- Family IS3
- Group IS3
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_009138 | ND | Herminiimonas arsenicoxydans | Herminiimonas arsenicoxydans |
DNA section
IS Length : 1249 bp
Ends
IR Length : 23/27
IRL : TGGCCTGTCCCTGACTTGAAAGACACCAAGATAAGGTGTTTTAATCAATC
IRR : TGGCCTGTCCCCAAACTGAAAGACACCGCATTAAGCTAACTGAGCTTGCT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GTGATTCGTTGGCGC | GTG | CGAGGTACCCCCATC | 3 |
DNA sequence
TGGCCTGTCCCTGACTTGAAAGACACCAAGATAAGGTGTTTTAATCAATCAGGAGGAAATAAATGGCAAATCGTAGACAGTTCAGCAAGGAGTTCAAGGT
AGAGGCGGTTAGGCTAGTTAAAGAACGAGGTGTTTCAATCGTGCAGGCCGCCAAAGACCTCAATGTGCACGAGAACGTATTGCGCAAATGGGTTCATGCC
GTATCGGAAGATGCATCGCATGCCTTTCCCGGGCATGGTCAACTCAAACCAGAGCAACTTGAGATAGCAAGGTTGCGCAAAGAAGTCGCCAAACTGAAGA
TGGAGCGCGACATTTTAAAAAAAGCCGCCGCCTACTTCGCCAGGGAATCGATGTGAAATTTGGATTCGTGGCGAAGCACCGAGGAATCTGGCCGGTGGCA
CTGACCTGCGACACACTCGGCGTCTCGCGCAGTGGTTTTTATGCATGGCTCACGCGTACGCCGTGCAAACGTCGCACGGAGAACGAGCAACTCGGTCGCG
CCGTTCATCACAGTTTTATTCAAAGTGACCGCACCTATGGAGCACGTCGTGTCTGGCACGACCTGTTGGCAAGTGGCTATCGTTGCGGGCTGCATCGCGT
CGAACGGCTGATGCAGGCTCAAGCGTTACGGGCTCGACCTCGCCGGCGCAGCTTGCCGATTGACCGAGGTGAGCGCCCAGTCATCGGTATTGCAGCCAAC
GTGCTCGACCGGCAATTCGACGCTAGTGCACCGAACCGGAAATGGGTCGCTGACTTCACCTACATCTGGTCAGCCGAAGGATGGCTTTACCTCGCTGTTG
TACTAGACCTCTATTCCAGGCGTGTCATTGGCTGGTCGATGAAGCCTGAGATGAATGCACAACTCGTCGCCGATGCATTAATGATGGCTGTATGGCGACG
TGGTAAGCCGGAATCCGTGATGCATCATTCTGACCGTGGAAGTCAATATACGAGTGAGCAGTTTCAGCGGCTATTGCTTGAATTGGGTGTGACGTGCAGC
ATGAGTCGTGCGGGTAATGTGTGGGACAACTCGGCCATGGAGAGCTTCTTCTCTTCATTGAAGACAGAGCGCTTATCCAGAAAGATGTTCCGCACGCGAG
ACGACATCAGGGCAGAAGTGTTTGACTACATCGAACGTTTCTATAATCCGGTTCGAAGACATTCAACGTTGGGCTATATCAGCCCAATTGACTTTGAACA
GCAAGCTCAGTTAGCTTAATGCGGTGTCTTTCAGTTTGGGGACAGGCCA
AGAGGCGGTTAGGCTAGTTAAAGAACGAGGTGTTTCAATCGTGCAGGCCGCCAAAGACCTCAATGTGCACGAGAACGTATTGCGCAAATGGGTTCATGCC
GTATCGGAAGATGCATCGCATGCCTTTCCCGGGCATGGTCAACTCAAACCAGAGCAACTTGAGATAGCAAGGTTGCGCAAAGAAGTCGCCAAACTGAAGA
TGGAGCGCGACATTTTAAAAAAAGCCGCCGCCTACTTCGCCAGGGAATCGATGTGAAATTTGGATTCGTGGCGAAGCACCGAGGAATCTGGCCGGTGGCA
CTGACCTGCGACACACTCGGCGTCTCGCGCAGTGGTTTTTATGCATGGCTCACGCGTACGCCGTGCAAACGTCGCACGGAGAACGAGCAACTCGGTCGCG
CCGTTCATCACAGTTTTATTCAAAGTGACCGCACCTATGGAGCACGTCGTGTCTGGCACGACCTGTTGGCAAGTGGCTATCGTTGCGGGCTGCATCGCGT
CGAACGGCTGATGCAGGCTCAAGCGTTACGGGCTCGACCTCGCCGGCGCAGCTTGCCGATTGACCGAGGTGAGCGCCCAGTCATCGGTATTGCAGCCAAC
GTGCTCGACCGGCAATTCGACGCTAGTGCACCGAACCGGAAATGGGTCGCTGACTTCACCTACATCTGGTCAGCCGAAGGATGGCTTTACCTCGCTGTTG
TACTAGACCTCTATTCCAGGCGTGTCATTGGCTGGTCGATGAAGCCTGAGATGAATGCACAACTCGTCGCCGATGCATTAATGATGGCTGTATGGCGACG
TGGTAAGCCGGAATCCGTGATGCATCATTCTGACCGTGGAAGTCAATATACGAGTGAGCAGTTTCAGCGGCTATTGCTTGAATTGGGTGTGACGTGCAGC
ATGAGTCGTGCGGGTAATGTGTGGGACAACTCGGCCATGGAGAGCTTCTTCTCTTCATTGAAGACAGAGCGCTTATCCAGAAAGATGTTCCGCACGCGAG
ACGACATCAGGGCAGAAGTGTTTGACTACATCGAACGTTTCTATAATCCGGTTCGAAGACATTCAACGTTGGGCTATATCAGCCCAATTGACTTTGAACA
GCAAGCTCAGTTAGCTTAATGCGGTGTCTTTCAGTTTGGGGACAGGCCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 294 bp | 97 aa | 63 | 356 | + | No |
Description : First part of the transposase
ORF sequence :
MANRRQFSKEFKVEAVRLVKERGVSIVQAAKDLNVHENVLRKWVHAVSEDASHAFPGHGQLKPEQLEIARLRKEVAKLKMERDILKKAAAYFARESM
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 957 bp | 318 aa | 263 | 1219 | + | No |
Description : Second part of the transposase
ORF sequence :
DSKVAQRSRQTEDGARHFKKSRRLLRQGIDVKFGFVAKHRGIWPVALTCDTLGVSRSGFYAWLTRTPCKRRTENEQLGRAVHHSFIQSDRTYGARRVWHD
LLASGYRCGLHRVERLMQAQALRARPRRRSLPIDRGERPVIGIAANVLDRQFDASAPNRKWVADFTYIWSAEGWLYLAVVLDLYSRRVIGWSMKPEMNAQ
LVADALMMAVWRRGKPESVMHHSDRGSQYTSEQFQRLLLELGVTCSMSRAGNVWDNSAMESFFSSLKTERLSRKMFRTRDDIRAEVFDYIERFYNPVRRH
STLGYISPIDFEQQAQLA
LLASGYRCGLHRVERLMQAQALRARPRRRSLPIDRGERPVIGIAANVLDRQFDASAPNRKWVADFTYIWSAEGWLYLAVVLDLYSRRVIGWSMKPEMNAQ
LVADALMMAVWRRGKPESVMHHSDRGSQYTSEQFQRLLLELGVTCSMSRAGNVWDNSAMESFFSSLKTERLSRKMFRTRDDIRAEVFDYIERFYNPVRRH
STLGYISPIDFEQQAQLA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1157 bp | 385 aa | 63 | 1219 | + | Yes |
Chemistry : DDE
ORF sequence :
MANRRQFSKEFKVEAVRLVKERGVSIVQAAKDLNVHENVLRKWVHAVSEDASHAFPGHGQLKPEQLEIARLRKEVAKLKMERDILKKSRRLLRQGIDVKF
GFVAKHRGIWPVALTCDTLGVSRSGFYAWLTRTPCKRRTENEQLGRAVHHSFIQSDRTYGARRVWHDLLASGYRCGLHRVERLMQAQALRARPRRRSLPI
DRGERPVIGIAANVLDRQFDASAPNRKWVADFTYIWSAEGWLYLAVVLDLYSRRVIGWSMKPEMNAQLVADALMMAVWRRGKPESVMHHSDRGSQYTSEQ
FQRLLLELGVTCSMSRAGNVWDNSAMESFFSSLKTERLSRKMFRTRDDIRAEVFDYIERFYNPVRRHSTLGYISPIDFEQQAQLA
GFVAKHRGIWPVALTCDTLGVSRSGFYAWLTRTPCKRRTENEQLGRAVHHSFIQSDRTYGARRVWHDLLASGYRCGLHRVERLMQAQALRARPRRRSLPI
DRGERPVIGIAANVLDRQFDASAPNRKWVADFTYIWSAEGWLYLAVVLDLYSRRVIGWSMKPEMNAQLVADALMMAVWRRGKPESVMHHSDRGSQYTSEQ
FQRLLLELGVTCSMSRAGNVWDNSAMESFFSSLKTERLSRKMFRTRDDIRAEVFDYIERFYNPVRRHSTLGYISPIDFEQQAQLA
Blast result :
Comments
ISHar2 is 80% aa similar to ISMlo4.
The third ORF is putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
The third ORF is putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Muller,D., Medigue,C., Koechler,S., Barbe,V., Barakat,M., Talla,E., Bonnefoy,V., Krin,E., Arsene-Ploetze,F., Carapito,C., Chandler,M., Cournoyer,B., Cruveiller,S., Dossat,C., Duval,S., Heymann,M., Leize,E., Lieutaud,A., Lievremont,D., Makita,Y., Mangenot,S., Nitschke,W., Ortet,P., Perdrial,N., Schoepp,B., Siguier,P., Simeonova,D.D., Rouy,Z., Segurens,B., Turlin,E., Vallenet,D., Van Dorsselaer,A., Weiss,S., Weissenbach,J., Lett,M.C., Danchin,A. and Bertin,P.N. (2007) PLoS Genet. 3 (4), E53