ISHar1
- Family IS3
- Group IS150
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_009138 | ND | Herminiimonas arsenicoxydans | Herminiimonas arsenicoxydans |
DNA section
IS Length : 1450 bp
Ends
IR Length : 20/24
IRL : TGAACTGAACCCCAAAAATTGGACACCAACTTTTGGGGTTTTTTAATGAC
IRR : TGAACTGACCCCAAATAGTTGGACGGTTTAGTTTGCTAAGCGGCCAAGGG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| AAAAGGGACGCAG | ATT | GCGTCCCTTTTTT | 3 |
DNA sequence
TGAACTGAACCCCAAAAATTGGACACCAACTTTTGGGGTTTTTTAATGACGAAATTCGACGAGAAATTCAAGATTTCGGTAGTAGAGCAGTATTTATCCG
GCACAGATGGATGTGCCATTGTGGGTCGCCGCAACGGCGTTTCGACAACGATGGTGACGCGGTGGGTGGCGTCCTATCAGAAGCACGGCATTGCTGGTTT
GAGGAAGAAATTCCAGCATTACGATGCGCAGTTCCGGTTGCGTGTGCTGCGGCAGATGTGGAAGAAGCAATGGTCGTATCAAGAGACGGGGCTCGCCTTT
GGGATTCGTAGCCAAGGATGCGTGGCCGTATGGGAGCGCTGCTATCATAACGGCGGTATAGACGCCCTCCAACCACGCAAACGTGGAAGACCACCAAGAA
TGCCTCATTCCCCCGTTACACCACTCCCGAGCTCCGTCAATGATGAAACCCGCAGCCGCGAGGATCTGTTGGCCGAGATCAATCAGCTGCGCATGGAGAA
TGCCTACTTAAAAAAGTTGGAAGCCTTAGTTCAAGCGCAGAAGCAACAGCGGGCAACAACGCGCGGAAATCGCAAGTAGTCCATGAACTAAGGCAACACT
TCCCGTTGGCCGATCTGCTCACGTTTGCTGAGCTACCGCGCAGCACTTTCTACTATCAGCAGAAGGTTGCGCAGGCGCCGGATAAGTATCAGCCGTTGAA
ACAACAAATCCAGTCCATCTTCGCCCGTCACCATGGGCGCTATGGCTATCGTCGCGTCACGGCTGCACTGGGCCGGCTGGGCCAGGGTGTCAATCACAAG
AAGGTGCAGCGCCTCATGCGGCAGTTGGGGCTCAAATCGTTGGTGCGTGCGAAGAAGTATCGTTCCTACAAAGGCGACGTCGGAAAAAGCGCGCCCAATG
CTTTGCAGCGTCAGTTCACAGCAGAGAAAGCCAATGAAAAATGGGTAACCGACGTCACGGAGTTCAATGTGGCGGGAGAAAAGCTGTATCTCTCGCCGGT
GATGGATTTATACAACGGCGAAATCATCGCCTTTGAAACGGCGAGGCGTCCGGTCTATGAACTGGTGGGAGTCATGCTGAAAAAAGCGCTCTCCAAGCTC
AAACCGGATGAGAGGCCGATGCTGCATTCAGACCAAGGCTGGCAATACAGGATGCCCGCTTACGCCAGGGTGCTACAGCAAAAAGGGATGGTGCAAAGTA
TGTCCCGTAAAGGGAACTGCCTCGATAACGCCGCCATGGAGAGCTTCTTTGCTGTGCTCAAATCAGAATTTTTTTACCTCAACAAGTTTCGCAGTATTGA
CGAGCTAACAGCGGGGCTCAAGGAGTACATTCGCTATTACAATCACGATCGCATCAAGATGAAACTAAAAGGACTGAGTCCTGTGCAATACAGAACCCAG
CCCTTGGCCGCTTAGCAAACTAAACCGTCCAACTATTTGGGGTCAGTTCA
GCACAGATGGATGTGCCATTGTGGGTCGCCGCAACGGCGTTTCGACAACGATGGTGACGCGGTGGGTGGCGTCCTATCAGAAGCACGGCATTGCTGGTTT
GAGGAAGAAATTCCAGCATTACGATGCGCAGTTCCGGTTGCGTGTGCTGCGGCAGATGTGGAAGAAGCAATGGTCGTATCAAGAGACGGGGCTCGCCTTT
GGGATTCGTAGCCAAGGATGCGTGGCCGTATGGGAGCGCTGCTATCATAACGGCGGTATAGACGCCCTCCAACCACGCAAACGTGGAAGACCACCAAGAA
TGCCTCATTCCCCCGTTACACCACTCCCGAGCTCCGTCAATGATGAAACCCGCAGCCGCGAGGATCTGTTGGCCGAGATCAATCAGCTGCGCATGGAGAA
TGCCTACTTAAAAAAGTTGGAAGCCTTAGTTCAAGCGCAGAAGCAACAGCGGGCAACAACGCGCGGAAATCGCAAGTAGTCCATGAACTAAGGCAACACT
TCCCGTTGGCCGATCTGCTCACGTTTGCTGAGCTACCGCGCAGCACTTTCTACTATCAGCAGAAGGTTGCGCAGGCGCCGGATAAGTATCAGCCGTTGAA
ACAACAAATCCAGTCCATCTTCGCCCGTCACCATGGGCGCTATGGCTATCGTCGCGTCACGGCTGCACTGGGCCGGCTGGGCCAGGGTGTCAATCACAAG
AAGGTGCAGCGCCTCATGCGGCAGTTGGGGCTCAAATCGTTGGTGCGTGCGAAGAAGTATCGTTCCTACAAAGGCGACGTCGGAAAAAGCGCGCCCAATG
CTTTGCAGCGTCAGTTCACAGCAGAGAAAGCCAATGAAAAATGGGTAACCGACGTCACGGAGTTCAATGTGGCGGGAGAAAAGCTGTATCTCTCGCCGGT
GATGGATTTATACAACGGCGAAATCATCGCCTTTGAAACGGCGAGGCGTCCGGTCTATGAACTGGTGGGAGTCATGCTGAAAAAAGCGCTCTCCAAGCTC
AAACCGGATGAGAGGCCGATGCTGCATTCAGACCAAGGCTGGCAATACAGGATGCCCGCTTACGCCAGGGTGCTACAGCAAAAAGGGATGGTGCAAAGTA
TGTCCCGTAAAGGGAACTGCCTCGATAACGCCGCCATGGAGAGCTTCTTTGCTGTGCTCAAATCAGAATTTTTTTACCTCAACAAGTTTCGCAGTATTGA
CGAGCTAACAGCGGGGCTCAAGGAGTACATTCGCTATTACAATCACGATCGCATCAAGATGAAACTAAAAGGACTGAGTCCTGTGCAATACAGAACCCAG
CCCTTGGCCGCTTAGCAAACTAAACCGTCCAACTATTTGGGGTCAGTTCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 534 bp | 177 aa | 46 | 579 | + | No |
Description : First part of the transposase
ORF sequence :
MTKFDEKFKISVVEQYLSGTDGCAIVGRRNGVSTTMVTRWVASYQKHGIAGLRKKFQHYDAQFRLRVLRQMWKKQWSYQETGLAFGIRSQGCVAVWERCY
HNGGIDALQPRKRGRPPRMPHSPVTPLPSSVNDETRSREDLLAEINQLRMENAYLKKLEALVQAQKQQRATTRGNRK
HNGGIDALQPRKRGRPPRMPHSPVTPLPSSVNDETRSREDLLAEINQLRMENAYLKKLEALVQAQKQQRATTRGNRK
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 969 bp | 322 aa | 447 | 1415 | + | No |
Description : Second part of the transposase
ORF sequence :
NPQPRGSVGRDQSAAHGECLLKKVGSLSSSAEATAGNNARKSQVVHELRQHFPLADLLTFAELPRSTFYYQQKVAQAPDKYQPLKQQIQSIFARHHGRYG
YRRVTAALGRLGQGVNHKKVQRLMRQLGLKSLVRAKKYRSYKGDVGKSAPNALQRQFTAEKANEKWVTDVTEFNVAGEKLYLSPVMDLYNGEIIAFETAR
RPVYELVGVMLKKALSKLKPDERPMLHSDQGWQYRMPAYARVLQQKGMVQSMSRKGNCLDNAAMESFFAVLKSEFFYLNKFRSIDELTAGLKEYIRYYNH
DRIKMKLKGLSPVQYRTQPLAA
YRRVTAALGRLGQGVNHKKVQRLMRQLGLKSLVRAKKYRSYKGDVGKSAPNALQRQFTAEKANEKWVTDVTEFNVAGEKLYLSPVMDLYNGEIIAFETAR
RPVYELVGVMLKKALSKLKPDERPMLHSDQGWQYRMPAYARVLQQKGMVQSMSRKGNCLDNAAMESFFAVLKSEFFYLNKFRSIDELTAGLKEYIRYYNH
DRIKMKLKGLSPVQYRTQPLAA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1370 bp | 456 aa | 46 | 1415 | + | Yes |
Chemistry : DDE
ORF sequence :
MTKFDEKFKISVVEQYLSGTDGCAIVGRRNGVSTTMVTRWVASYQKHGIAGLRKKFQHYDAQFRLRVLRQMWKKQWSYQETGLAFGIRSQGCVAVWERCY
HNGGIDALQPRKRGRPPRMPHSPVTPLPSSVNDETRSREDLLAEINQLRMENAYLKKVGSLSSSAEATAGNNARKSQVVHELRQHFPLADLLTFAELPRS
TFYYQQKVAQAPDKYQPLKQQIQSIFARHHGRYGYRRVTAALGRLGQGVNHKKVQRLMRQLGLKSLVRAKKYRSYKGDVGKSAPNALQRQFTAEKANEKW
VTDVTEFNVAGEKLYLSPVMDLYNGEIIAFETARRPVYELVGVMLKKALSKLKPDERPMLHSDQGWQYRMPAYARVLQQKGMVQSMSRKGNCLDNAAMES
FFAVLKSEFFYLNKFRSIDELTAGLKEYIRYYNHDRIKMKLKGLSPVQYRTQPLAA
HNGGIDALQPRKRGRPPRMPHSPVTPLPSSVNDETRSREDLLAEINQLRMENAYLKKVGSLSSSAEATAGNNARKSQVVHELRQHFPLADLLTFAELPRS
TFYYQQKVAQAPDKYQPLKQQIQSIFARHHGRYGYRRVTAALGRLGQGVNHKKVQRLMRQLGLKSLVRAKKYRSYKGDVGKSAPNALQRQFTAEKANEKW
VTDVTEFNVAGEKLYLSPVMDLYNGEIIAFETARRPVYELVGVMLKKALSKLKPDERPMLHSDQGWQYRMPAYARVLQQKGMVQSMSRKGNCLDNAAMES
FFAVLKSEFFYLNKFRSIDELTAGLKEYIRYYNHDRIKMKLKGLSPVQYRTQPLAA
Blast result :
Comments
ISHar1 is 76% aa similar to ISBam1.
The third ORF is putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
The third ORF is putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Muller,D., Medigue,C., Koechler,S., Barbe,V., Barakat,M., Talla,E., Bonnefoy,V., Krin,E., Arsene-Ploetze,F., Carapito,C., Chandler,M., Cournoyer,B., Cruveiller,S., Dossat,C., Duval,S., Heymann,M., Leize,E., Lieutaud,A., Lievremont,D., Makita,Y., Mangenot,S., Nitschke,W., Ortet,P., Perdrial,N., Schoepp,B., Siguier,P., Simeonova,D.D., Rouy,Z., Segurens,B., Turlin,E., Vallenet,D., Van Dorsselaer,A., Weiss,S., Weissenbach,J., Lett,M.C., Danchin,A. and Bertin,P.N. (2007) PLoS Genet. 3 (4), E53.