ISRhsp4
- Family IS5
- Group IS427
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_009430 | ND | Rhodobacter sphaeroides | Rhodobacter sphaeroides ATCC 17025 plasmid pRSPA02 |
DNA section
IS Length : 840 bp
Ends
IR Length : 13/15
IRL : GGGTCAGGACCCATTGATCTTGCTTCTGCGGCGTGATTCAGCCCCCGCAA
IRR : GGGTCAGGACTCGTTCTGGATCACAGCCAGAACATGACGGTCGCGGCGAG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CTCAGGGCAGGAGCC | TA | CGCACTTCTCC | 2 |
DNA sequence
GGGTCAGGACCCATTGATCTTGCTTCTGCGGCGTGATTCAGCCCCCGCAAGGAGACTGACCGATGAGCAACCTCTACTGGCTGTCCGAGGCGCAGATGGA
GCGCCTGAAGCCGTTCCTGCCCAAGAGCCACGGCAAGCCCCGCGTCGACGACCGGCGTGTGCTAAGCGGCATAATCTTCATTAACCGCAATGGTTTGCGG
TGGTGCGACGCGCCGAAGGAGTATGGGCCCGCGAAGACCCTGTACAACCGCTGGAAGCGCTGGAGCGACAACGGTGTCTTCGCCCGGATCATGATGGGCC
TGGCCGCCGAGAGGGCCGAGCACAAGACGATCATGATCGACGCGACCTACCTGAAAGCGCATCGCACGGCCTCCAGCCTGCGTGTGAAAAAGGGGGGCGC
GGACGCCAGATCGGGCGGACCAAGGGCGGCATGAACACCAAGCTGCACGCCGTCGCCGATGCGAACGGCCGGCCGATCGGGTTCTTCCTGTCGGCCGGTC
CAGTCAGCGACTACACTGGTGCGGCGGCGCTGCTGGGCAGCCTCCCGAAAGCGGACTGGCTGCTGGCGGACAGGGGTTACGATGCCGATTGGCTCAGAGA
AGCGTTGAAACACAAGGGGATGAAGGTCTGCATCCCAGGCCGGAAGTCCCGCAAGAAGGCCGTGAGATACGACAAGCGACGCTACAAGCGCCGCAACCGC
ATCGAGATCATGTTTGGCCGCCTCAAGGATTGGCGGCGGGTGGCCACCCGGTACGACCGTTGCCCGCAAACCTTCTTCTCAGCCATCGCTCTCGCCGCGA
CCGTCATGTTCTGGCTGTGATCCAGAACGAGTCCTGACCC
GCGCCTGAAGCCGTTCCTGCCCAAGAGCCACGGCAAGCCCCGCGTCGACGACCGGCGTGTGCTAAGCGGCATAATCTTCATTAACCGCAATGGTTTGCGG
TGGTGCGACGCGCCGAAGGAGTATGGGCCCGCGAAGACCCTGTACAACCGCTGGAAGCGCTGGAGCGACAACGGTGTCTTCGCCCGGATCATGATGGGCC
TGGCCGCCGAGAGGGCCGAGCACAAGACGATCATGATCGACGCGACCTACCTGAAAGCGCATCGCACGGCCTCCAGCCTGCGTGTGAAAAAGGGGGGCGC
GGACGCCAGATCGGGCGGACCAAGGGCGGCATGAACACCAAGCTGCACGCCGTCGCCGATGCGAACGGCCGGCCGATCGGGTTCTTCCTGTCGGCCGGTC
CAGTCAGCGACTACACTGGTGCGGCGGCGCTGCTGGGCAGCCTCCCGAAAGCGGACTGGCTGCTGGCGGACAGGGGTTACGATGCCGATTGGCTCAGAGA
AGCGTTGAAACACAAGGGGATGAAGGTCTGCATCCCAGGCCGGAAGTCCCGCAAGAAGGCCGTGAGATACGACAAGCGACGCTACAAGCGCCGCAACCGC
ATCGAGATCATGTTTGGCCGCCTCAAGGATTGGCGGCGGGTGGCCACCCGGTACGACCGTTGCCCGCAAACCTTCTTCTCAGCCATCGCTCTCGCCGCGA
CCGTCATGTTCTGGCTGTGATCCAGAACGAGTCCTGACCC
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 372 bp | 123 aa | 63 | 434 | + | No |
Description : First part of the transposase
ORF sequence :
MSNLYWLSEAQMERLKPFLPKSHGKPRVDDRRVLSGIIFINRNGLRWCDAPKEYGPAKTLYNRWKRWSDNGVFARIMMGLAAERAEHKTIMIDATYLKAH
RTASSLRVKKGGADARSGGPRAA
RTASSLRVKKGGADARSGGPRAA
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 636 bp | 211 aa | 185 | 820 | + | No |
Description : Second part of the transposase
ORF sequence :
PQWFAVVRRAEGVWAREDPVQPLEALERQRCLRPDHDGPGRREGRAQDDHDRRDLPESASHGLQPACEKGGRGRQIGRTKGGMNTKLHAVADANGRPIGF
FLSAGPVSDYTGAAALLGSLPKADWLLADRGYDADWLREALKHKGMKVCIPGRKSRKKAVRYDKRRYKRRNRIEIMFGRLKDWRRVATRYDRCPQTFFSA
IALAATVMFWL
FLSAGPVSDYTGAAALLGSLPKADWLLADRGYDADWLREALKHKGMKVCIPGRKSRKKAVRYDKRRYKRRNRIEIMFGRLKDWRRVATRYDRCPQTFFSA
IALAATVMFWL
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 758 bp | 252 aa | 63 | 820 | + | Yes |
Chemistry : DDE
ORF sequence :
MSNLYWLSEAQMERLKPFLPKSHGKPRVDDRRVLSGIIFINRNGLRWCDAPKEYGPAKTLYNRWKRWSDNGVFARIMMGLAAERAEHKTIMIDATYLKAH
RTASSLRVKKGGRGRQIGRTKGGMNTKLHAVADANGRPIGFFLSAGPVSDYTGAAALLGSLPKADWLLADRGYDADWLREALKHKGMKVCIPGRKSRKKA
VRYDKRRYKRRNRIEIMFGRLKDWRRVATRYDRCPQTFFSAIALAATVMFWL
RTASSLRVKKGGRGRQIGRTKGGMNTKLHAVADANGRPIGFFLSAGPVSDYTGAAALLGSLPKADWLLADRGYDADWLREALKHKGMKVCIPGRKSRKKA
VRYDKRRYKRRNRIEIMFGRLKDWRRVATRYDRCPQTFFSAIALAATVMFWL
Blast result :
Comments
ISRhsp4 is 87% aa similar to IS1248A.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina del Rio,T., Hammon,N., Israni,S., Dalin,E., Tice,H., Pitluck,S., Chertkov,O., Brettin,T., Bruce,D., Han,C., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Kim,E. and Richardson,P. (2007) Direct submission GenBank.