ISRhosp5
- Family IS3
- Group IS51
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_008268 | ND | Rhodococcus sp. | Rhodococcus jostii RHA1 Rhodococcus jostii RHA1 plasmid pRHL1 Rhodococcus sp. Rhodococcus jostii RHA1 plasmid pRHL3 |
DNA section
IS Length : 1335 bp
Ends
IR Length : 19/25
IRL : GGGGGTTCCCCCCTGAGTGAGGACATCGTGAACACTGGGTCGTGAAGGTC
IRR : GGGCGTTCCCCCCGAGATGTGGACACCAATTCCTATGCAGCGGTGGCCAG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CGACATCGAT | CAA | AGTGGTGATG | 3 |
| TGAGGCTGCA | CCC | TCCCACATCT | 3 |
| TGCGGACGAT | GGG | CAACGCAACC | 3 |
| GAACCACGCT | TCG | CCCGGCTGTT | 3 |
| TGAGTCGGGG | GAC | CTTGTAGTTG | 3 |
DNA sequence
GGGGGTTCCCCCCTGAGTGAGGACATCGTGAACACTGGGTCGTGAAGGTCCCAGAGGAAGAGATGTCACTGATGGCGATGAAGGCGTACTCGGCGGAGTT
CAAGGCCGATGCCGTCGCGCTGTACCTGTCCGACCCGAGCCACACCTTTGAGGGCATCGGCAACGACCTGGGAGTCAGCCGCGAGACCCTGCGCAACTGG
GTGCGGGCCGAACGCAAACGCACCGGTACCTCCACGGCCGAGCTCCGGGCCGACGGGGCTGCGCGGCCGGCATCCCGGGCCGGCGAGGTATCGTCCGAGT
CCGTGTTGGAGGAAGAAAACAAGCAGCTCAAGGCCCAAATCCGGAAGCTCGAAACCGAGCGGGAGATCCTGCGCAAGGCCGCGAAGTATTTCGCGGGCGA
GACGAATTGGTGAGCCGCTTCCAGTTCGTTGACGACCACTGCGACACCGTTCCGGTGAAGTGGCTGTGCCAGATCCTCGAAGTCTCCCGCTCGGGCTTCT
ACCGGTGGCGGACCTCCGGACCGGCCCGGGCCGCCCGTGCCCGCGCCGACGAGGAGCTGGCCGAGCGGATCCGCGCTATCCATGCCGACTTCGATGGCAC
CTACGGTGCCCCGCGCATCACGGCCGAACTGCGCGAGGCCGGGATCGAGGTCAATCACAAGCGGGTCGAGCGGGTGATGCGTGAGCACGGGATCGTCGGG
GTGCACCTGCGCAAACCGGTGCGCACCACCGTCCCCGATCCGGACGCGGCCGCGGTGCCGGACCTGATCCGGCGGGACTTCACCGCGAGCGCACCGAACA
CTCGATACGTCGGCGACATCACCTACCTCCCAGTCGGTGACGGTGAATTTCTGTATCTGGCGACGGTGTTGGACCTGGGCTCGCGACGGTTGGCGGGGTG
GTCGATCGCCGACCACATGCGTACCGAGTTGGTCACCGATGCGATGCGGGCGGCGGCGGCCTGTCGCGGCGCCGCAGGGCTCGAGGGGTCAATTTTCCAC
TCCGACAACGGAGCCCAGTATGCATCGGCCGAGTTCGCCGACCTCTGCCGCGAGCTGGGGGTGACCCGGTCGCGGGGTGCGGTCGGAACGTCGGCGGACA
ACGCGGCCGCCGAGTCGCTGAACGCGACGTTGAAACGGGAGACGCTGCAGGGGCGGAAGCGCTGGAACAGTGCGGGTGAGGCCCGCGCCGCCATCTTCCG
CTGGATCACCCGCTACAACACGCGAAGGAGGCATTCCACCCTCGGTCAGATCTGTCCGATCGAGTTCGAGCAGCGATCGGCTACGCTGGCCACCGCTGCA
TAGGAATTGGTGTCCACATCTCGGGGGGAACGCCC
CAAGGCCGATGCCGTCGCGCTGTACCTGTCCGACCCGAGCCACACCTTTGAGGGCATCGGCAACGACCTGGGAGTCAGCCGCGAGACCCTGCGCAACTGG
GTGCGGGCCGAACGCAAACGCACCGGTACCTCCACGGCCGAGCTCCGGGCCGACGGGGCTGCGCGGCCGGCATCCCGGGCCGGCGAGGTATCGTCCGAGT
CCGTGTTGGAGGAAGAAAACAAGCAGCTCAAGGCCCAAATCCGGAAGCTCGAAACCGAGCGGGAGATCCTGCGCAAGGCCGCGAAGTATTTCGCGGGCGA
GACGAATTGGTGAGCCGCTTCCAGTTCGTTGACGACCACTGCGACACCGTTCCGGTGAAGTGGCTGTGCCAGATCCTCGAAGTCTCCCGCTCGGGCTTCT
ACCGGTGGCGGACCTCCGGACCGGCCCGGGCCGCCCGTGCCCGCGCCGACGAGGAGCTGGCCGAGCGGATCCGCGCTATCCATGCCGACTTCGATGGCAC
CTACGGTGCCCCGCGCATCACGGCCGAACTGCGCGAGGCCGGGATCGAGGTCAATCACAAGCGGGTCGAGCGGGTGATGCGTGAGCACGGGATCGTCGGG
GTGCACCTGCGCAAACCGGTGCGCACCACCGTCCCCGATCCGGACGCGGCCGCGGTGCCGGACCTGATCCGGCGGGACTTCACCGCGAGCGCACCGAACA
CTCGATACGTCGGCGACATCACCTACCTCCCAGTCGGTGACGGTGAATTTCTGTATCTGGCGACGGTGTTGGACCTGGGCTCGCGACGGTTGGCGGGGTG
GTCGATCGCCGACCACATGCGTACCGAGTTGGTCACCGATGCGATGCGGGCGGCGGCGGCCTGTCGCGGCGCCGCAGGGCTCGAGGGGTCAATTTTCCAC
TCCGACAACGGAGCCCAGTATGCATCGGCCGAGTTCGCCGACCTCTGCCGCGAGCTGGGGGTGACCCGGTCGCGGGGTGCGGTCGGAACGTCGGCGGACA
ACGCGGCCGCCGAGTCGCTGAACGCGACGTTGAAACGGGAGACGCTGCAGGGGCGGAAGCGCTGGAACAGTGCGGGTGAGGCCCGCGCCGCCATCTTCCG
CTGGATCACCCGCTACAACACGCGAAGGAGGCATTCCACCCTCGGTCAGATCTGTCCGATCGAGTTCGAGCAGCGATCGGCTACGCTGGCCACCGCTGCA
TAGGAATTGGTGTCCACATCTCGGGGGGAACGCCC
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 342 bp | 113 aa | 72 | 413 | + | No |
Description : First part of the transposase
ORF sequence :
MAMKAYSAEFKADAVALYLSDPSHTFEGIGNDLGVSRETLRNWVRAERKRTGTSTAELRADGAARPASRAGEVSSESVLEEENKQLKAQIRKLETEREIL
RKAAKYFAGETNW
RKAAKYFAGETNW
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1155 bp | 383 aa | 149 | 1303 | + | No |
Description : Second part of the transposase
ORF sequence :
GHRQRPGSQPRDPAQLGAGRTQTHRYLHGRAPGRRGCAAGIPGRRGIVRVRVGGRKQAAQGPNPEARNRAGDPAQGREVFRGRDELVSRFQFVDDHCDTV
PVKWLCQILEVSRSGFYRWRTSGPARAARARADEELAERIRAIHADFDGTYGAPRITAELREAGIEVNHKRVERVMREHGIVGVHLRKPVRTTVPDPDAA
AVPDLIRRDFTASAPNTRYVGDITYLPVGDGEFLYLATVLDLGSRRLAGWSIADHMRTELVTDAMRAAAACRGAAGLEGSIFHSDNGAQYASAEFADLCR
ELGVTRSRGAVGTSADNAAAESLNATLKRETLQGRKRWNSAGEARAAIFRWITRYNTRRRHSTLGQICPIEFEQRSATLATAA
PVKWLCQILEVSRSGFYRWRTSGPARAARARADEELAERIRAIHADFDGTYGAPRITAELREAGIEVNHKRVERVMREHGIVGVHLRKPVRTTVPDPDAA
AVPDLIRRDFTASAPNTRYVGDITYLPVGDGEFLYLATVLDLGSRRLAGWSIADHMRTELVTDAMRAAAACRGAAGLEGSIFHSDNGAQYASAEFADLCR
ELGVTRSRGAVGTSADNAAAESLNATLKRETLQGRKRWNSAGEARAAIFRWITRYNTRRRHSTLGQICPIEFEQRSATLATAA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1232 bp | 410 aa | 72 | 1303 | + | Yes |
Chemistry : DDE
ORF sequence :
MAMKAYSAEFKADAVALYLSDPSHTFEGIGNDLGVSRETLRNWVRAERKRTGTSTAELRADGAARPASRAGEVSSESVLEEENKQLKAQIRKLETEREIL
RKAAKYFRGRDELVSRFQFVDDHCDTVPVKWLCQILEVSRSGFYRWRTSGPARAARARADEELAERIRAIHADFDGTYGAPRITAELREAGIEVNHKRVE
RVMREHGIVGVHLRKPVRTTVPDPDAAAVPDLIRRDFTASAPNTRYVGDITYLPVGDGEFLYLATVLDLGSRRLAGWSIADHMRTELVTDAMRAAAACRG
AAGLEGSIFHSDNGAQYASAEFADLCRELGVTRSRGAVGTSADNAAAESLNATLKRETLQGRKRWNSAGEARAAIFRWITRYNTRRRHSTLGQICPIEFE
QRSATLATAA
RKAAKYFRGRDELVSRFQFVDDHCDTVPVKWLCQILEVSRSGFYRWRTSGPARAARARADEELAERIRAIHADFDGTYGAPRITAELREAGIEVNHKRVE
RVMREHGIVGVHLRKPVRTTVPDPDAAAVPDLIRRDFTASAPNTRYVGDITYLPVGDGEFLYLATVLDLGSRRLAGWSIADHMRTELVTDAMRAAAACRG
AAGLEGSIFHSDNGAQYASAEFADLCRELGVTRSRGAVGTSADNAAAESLNATLKRETLQGRKRWNSAGEARAAIFRWITRYNTRRRHSTLGQICPIEFE
QRSATLATAA
Blast result :
Comments
ISRhosp5 is 60% (ORFA) aa similar to IS3502 and 63% (ORFB) aa similar to ISRhosp1. The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
The ends are not the classic for this family.
File updated: September 9 2013.
The ends are not the classic for this family.
File updated: September 9 2013.
References
1] McLeod,M.P., Warren,R.L., Araki,N., Hsiao,W.W.L., Myhre,M.,Fernandes,C., Miyazawa,D., Wong,W., Lillquist,A.L., Wang,D., Dosanjh,M., Petrescu,A., Morin,R.D., Yang,G., Stott,J.M., Schein,J.E., Shin,H., Smailus,D., Siddiqui,A.S., Marra,M.A., Jones,S.J.M., Holt,R., Brinkman,F.S.L., Miyauchi,K., Fukuda,M.,Davies,J.E., Mohn,W.W. and Eltis,L.D. (2006) Direct Submission GenBank.