ISRme15
- Family IS3
- Group IS51
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP000354 | ND | Ralstonia metallidurans | Ralstonia metallidurans CH34 plasmid pMOL30 |
DNA section
IS Length : 1325 bp
Ends
IR Length : 26/29
IRL : TGAATCGCCCCGGGTTTTGCGGAGGCTCTCACTCTTGAGAGAATGCGAGC
IRR : TGAATCGCCCCGGTTTTCGCGGAGGCTGTTTGGTTAAAGTAAAACGGCCT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CCAGGCCTGGCTGCTGC | GGC | GATCACGCAGG | 3 |
DNA sequence
TGAATCGCCCCGGGTTTTGCGGAGGCTCTCACTCTTGAGAGAATGCGAGCCATGAGCAAGAACAACGCAAACAAGTTTTCCCCGGAAGTGCGCGAGCGCG
CCGTGCGCCTGGTGCAGGAGTCGCGCGGCGAGTACCCGTCGCTGTGGGTGGCGGTCGAGTCCATCGCGCCCAAGATCGGCTGCTCGGCGCAGACGCTGCT
GACCTGGGTCAAGCGCCACGAAGTCGACAGCGGACAGCGCGAAGGCGTCACCACCAGCGAGCGAGAACGCATCAAGGAGTTGGAGCGGGAGAACCGAGAG
CTGCGCCGCGCCAACGACATCCTGCGCACTGCCAGCGCTTTTTTCGCGCAGGCGGAGCTCGACCGCAAGCTGAAGTCGTAAACACCTACATCGACCGGCA
CCGGGAGGTTTACGGGGTCGAGCCGATCTGCAAGGTGTTGCAGGTTGCCTCGTCGGCCTATCGGCGCCATGCCGCACGGCTGCGTGATCCGTCACGGCGC
AGCGACCGTGCGCGTCGTGATGAACGGCTCATGCCGCAAGTCCAGCGAGTGTGGCAGGAGAACCATCGCGTCTACGGCGCCGACAAGGTCTGGCGGCAAC
TGAACCGCGAGGGCGTGACGGTTGCCCGCTGCACGGTCGAGCGATTGATGCGCGCCCAGGGACTGCAAGGTGTGCGGCGCGGCAAGCGGCTGCGAACGAC
CATCGCTGACGACGCCGCCAGTCGCCCGGTGGATCGCGTCAACCGGCAGTTCCGGGCCGACCGTCCCAATCAGCTCTGGGTCTCGGACTTCACCTACGTC
TCGACATGGCAGGGTTGGCTGTACGTGGCCTTCGTCGTCGACGTCTACGCCAGGCGCATCGTCGGCTGGCGCGTGAGCAAATCCATGACGACGGACTTCG
TGCTCGATGCGCTGGAGCAGGCCCTATACGCTCGCCAGCCTGGCAACGACGGTTCTCTGACCCACCACTCCGATAGGGGATCGCAGTACGTCAGCATCCG
CTATAGCGAACGCCTGGCCGAAGCCGGCATCGAGCCGTCGGTCGGCAGCCGTGGCGACAGTTACGACAACGCCTTGGCCGAGACGATCAATGGCTTGTAC
AAGGCCGAGCTGATCCATCGCCGCGCGCCTTGGAAAACCAGGGAATCCGTCGAACTGGCAACACTGGAATGGGTCGCTTGGTTCAACCATAAGCGCCTGC
ACTCATCCATCGGCTATATCCCGCCCGCCGAGGCTGAGGCAAACTACTACAACCAACTCGGCAAAACCGCCGACGAGGCCGTTTTACTTTAACCAAACAG
CCTCCGCGAAAACCGGGGCGATTCA
CCGTGCGCCTGGTGCAGGAGTCGCGCGGCGAGTACCCGTCGCTGTGGGTGGCGGTCGAGTCCATCGCGCCCAAGATCGGCTGCTCGGCGCAGACGCTGCT
GACCTGGGTCAAGCGCCACGAAGTCGACAGCGGACAGCGCGAAGGCGTCACCACCAGCGAGCGAGAACGCATCAAGGAGTTGGAGCGGGAGAACCGAGAG
CTGCGCCGCGCCAACGACATCCTGCGCACTGCCAGCGCTTTTTTCGCGCAGGCGGAGCTCGACCGCAAGCTGAAGTCGTAAACACCTACATCGACCGGCA
CCGGGAGGTTTACGGGGTCGAGCCGATCTGCAAGGTGTTGCAGGTTGCCTCGTCGGCCTATCGGCGCCATGCCGCACGGCTGCGTGATCCGTCACGGCGC
AGCGACCGTGCGCGTCGTGATGAACGGCTCATGCCGCAAGTCCAGCGAGTGTGGCAGGAGAACCATCGCGTCTACGGCGCCGACAAGGTCTGGCGGCAAC
TGAACCGCGAGGGCGTGACGGTTGCCCGCTGCACGGTCGAGCGATTGATGCGCGCCCAGGGACTGCAAGGTGTGCGGCGCGGCAAGCGGCTGCGAACGAC
CATCGCTGACGACGCCGCCAGTCGCCCGGTGGATCGCGTCAACCGGCAGTTCCGGGCCGACCGTCCCAATCAGCTCTGGGTCTCGGACTTCACCTACGTC
TCGACATGGCAGGGTTGGCTGTACGTGGCCTTCGTCGTCGACGTCTACGCCAGGCGCATCGTCGGCTGGCGCGTGAGCAAATCCATGACGACGGACTTCG
TGCTCGATGCGCTGGAGCAGGCCCTATACGCTCGCCAGCCTGGCAACGACGGTTCTCTGACCCACCACTCCGATAGGGGATCGCAGTACGTCAGCATCCG
CTATAGCGAACGCCTGGCCGAAGCCGGCATCGAGCCGTCGGTCGGCAGCCGTGGCGACAGTTACGACAACGCCTTGGCCGAGACGATCAATGGCTTGTAC
AAGGCCGAGCTGATCCATCGCCGCGCGCCTTGGAAAACCAGGGAATCCGTCGAACTGGCAACACTGGAATGGGTCGCTTGGTTCAACCATAAGCGCCTGC
ACTCATCCATCGGCTATATCCCGCCCGCCGAGGCTGAGGCAAACTACTACAACCAACTCGGCAAAACCGCCGACGAGGCCGTTTTACTTTAACCAAACAG
CCTCCGCGAAAACCGGGGCGATTCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 339 bp | 112 aa | 43 | 381 | + | No |
Description : First part of the transposase
ORF sequence :
MRAMSKNNANKFSPEVRERAVRLVQESRGEYPSLWVAVESIAPKIGCSAQTLLTWVKRHEVDSGQREGVTTSERERIKELERENRELRRANDILRTASAF
FAQAELDRKLKS
FAQAELDRKLKS
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 990 bp | 329 aa | 303 | 1292 | + | No |
Description : Second part of the transposase
ORF sequence :
APRQRHPAHCQRFFRAGGARPQAEVVNTYIDRHREVYGVEPICKVLQVASSAYRRHAARLRDPSRRSDRARRDERLMPQVQRVWQENHRVYGADKVWRQL
NREGVTVARCTVERLMRAQGLQGVRRGKRLRTTIADDAASRPVDRVNRQFRADRPNQLWVSDFTYVSTWQGWLYVAFVVDVYARRIVGWRVSKSMTTDFV
LDALEQALYARQPGNDGSLTHHSDRGSQYVSIRYSERLAEAGIEPSVGSRGDSYDNALAETINGLYKAELIHRRAPWKTRESVELATLEWVAWFNHKRLH
SSIGYIPPAEAEANYYNQLGKTADEAVLL
NREGVTVARCTVERLMRAQGLQGVRRGKRLRTTIADDAASRPVDRVNRQFRADRPNQLWVSDFTYVSTWQGWLYVAFVVDVYARRIVGWRVSKSMTTDFV
LDALEQALYARQPGNDGSLTHHSDRGSQYVSIRYSERLAEAGIEPSVGSRGDSYDNALAETINGLYKAELIHRRAPWKTRESVELATLEWVAWFNHKRLH
SSIGYIPPAEAEANYYNQLGKTADEAVLL
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1250 bp | 416 aa | 43 | 1292 | + | Yes |
Chemistry : DDE
ORF sequence :
MRAMSKNNANKFSPEVRERAVRLVQESRGEYPSLWVAVESIAPKIGCSAQTLLTWVKRHEVDSGQREGVTTSERERIKELERENRELRRANDILRTASAF
FRAGGARPQAEVVNTYIDRHREVYGVEPICKVLQVASSAYRRHAARLRDPSRRSDRARRDERLMPQVQRVWQENHRVYGADKVWRQLNREGVTVARCTVE
RLMRAQGLQGVRRGKRLRTTIADDAASRPVDRVNRQFRADRPNQLWVSDFTYVSTWQGWLYVAFVVDVYARRIVGWRVSKSMTTDFVLDALEQALYARQP
GNDGSLTHHSDRGSQYVSIRYSERLAEAGIEPSVGSRGDSYDNALAETINGLYKAELIHRRAPWKTRESVELATLEWVAWFNHKRLHSSIGYIPPAEAEA
NYYNQLGKTADEAVLL
FRAGGARPQAEVVNTYIDRHREVYGVEPICKVLQVASSAYRRHAARLRDPSRRSDRARRDERLMPQVQRVWQENHRVYGADKVWRQLNREGVTVARCTVE
RLMRAQGLQGVRRGKRLRTTIADDAASRPVDRVNRQFRADRPNQLWVSDFTYVSTWQGWLYVAFVVDVYARRIVGWRVSKSMTTDFVLDALEQALYARQP
GNDGSLTHHSDRGSQYVSIRYSERLAEAGIEPSVGSRGDSYDNALAETINGLYKAELIHRRAPWKTRESVELATLEWVAWFNHKRLHSSIGYIPPAEAEA
NYYNQLGKTADEAVLL
Blast result :
Comments
ISRme15 is 90% aa similar to IS401(ORFA) and 88% to IS1416 (ORFB). The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina del Rio,T., Hammon,N., Israni,S., Dalin,E., Tice,H., Martinez,M., Goltsman,E., Pitluck,S., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Kim,E., Mergeay,M., Benotmane,M.A., Vallaeys,T., Michaux,A., Monchy,S., Dunn,J., McCorkle,S., Taghavi,S., van der Lelie,D. and Richardson,P. (2006) Direct submission GenBank.