ISPst3

  • Family IS21
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
AB088753 ND Pseudomonas stutzeri
Pseudomonas stutzeri OM1
Pseudomonas aeruginosa ST233
DNA section
IS Length : 2606 bp

Ends


IR Length : 23/27

IRL : TGTTGCCGCTGACCGAAAACTGACCCAGTAGAGGCTGTTCTGCCGACTGA
IRR : TGTTGGCGCTGATGGAAAATTGACCCACCCTGCCGATTGAAATTTGACCC

Insertion site


Left flankDirect repeatRight flankDR Length
AGACGTCACTGTGCTGCTGCATGG0
GACCCTGGCGAGAATCCAGTCATGGATCTCGCTTTCAAGC9

DNA sequence

TGTTGCCGCTGACCGAAAACTGACCCAGTAGAGGCTGTTCTGCCGACTGAAAACTGACCCAGGTGTTCAACTGCTTCTGCTCAATTTTTGAGCAGGAGAA
CACAGGGTGATCAGTATGGAAATGATGGGCAAAATTCGGCGGATGTATTTCCGCGACAAGCTGTCGCTGCATGAGATAGCCAAGCGCACCGGGCTGGCGC
GCAACACGATTCGCAAGTGGGTCAGAGCACCTGAGGCCAAGCAGCCGGTGTACCAACGCCGCGCGATCTTTAACAAACTCAGCCCTTTTCACGTCACGCC
AGAGCAGGCGCTAAAAGCCGATTCGCTGCGCCCCAAGCAACAGCGGCGCAGTGCCAAGGCGCTGTTGGCGCAAATCAAAGCCGATGGTTATGACGGTGGC
TACAGCCAGCTCACCGCGTTTATCCGTGCCTGGCGAGGGGGACAGGGCAAGGCGTCGCAGGCCTTTGTGCCGCTGACCTTTGCTCTTGGCGAGGCGTTTC
AGTTTGACTGGAGCGAGGAAGGCTTGCTGGTCGGCGGCATTTACCGGCGTATGCAGGTGGCACATCTGAAGCTGTGTGCCAGCCGTGCGTTCTGGCTTGT
GGCGTATCCGAGCCAGGGCCATGAGATGTTATTTGACGCCCATACACGCTCGTTCGGCGCCTTGGGCGGCGTGCCGCGCCGGGGCATCTACGACAACATG
AAGACCGCCGTCGACAAGGTCAATAAGGGCAAAGGCCGGGCGGTGAATGCACGCTTTGCGGTGATGTGCGCGCATTACCTGTTCGATCCGGACTTCTGCA
ACGTCGCCGCTGGTTGGGAAAAGGGCATCGTTGAAAAGAACGTGCAAGACAGCCGCAGGCGTATCTGGCTAGACGCCCAGGACTGTCAGTTTCACTCCTT
CGAGGAACTCAATGCCTGGCTGGGCCAGCGCTGCCGCGCGCTTTGGAACGAGCTGACGCACCCTCAATACAGCGGGCTGAGTGTGGCCGAAGTGCTTGAG
TTGGAGCGCGCTGAACTGATGCCTGTGCCAGCGCCATTCGACGGTTACGTCGAGCGGCCTGCGCGGGTCTCCAGCACCTGCTTGGTCAGCGTCGGGCGCA
ACCGCTACTCGGTGCCGTGTGAGTACGCGGGTAAGTGGGTCAGCAGCCGTTTGTATCCGACGCGAATCGAGGTGGTGGCTGACGACGCGCTGATCGCCAG
CCATGTCCGCTTGCTGGATCGCGACCAGGTCAGCTATGACTGGCAGCACTACCTCCCGCTGATCGAACGCAAGCCCGGTGCGCTGCGCAACGGCGCGCCC
TTTGCTGATCTGCCGGCGCCGCTGCGTCAGCTTAAGCACGGTCTGGGGCGTCATGCCGGCGGTGACCGGATCATGGCGCAAGTTCTGGCTGCCGTACCGG
TCGCCGGGCTCGATGCCGTGCTGGTAGCCGTTGAGCTGGTACTGGAGAGCGGCAGCCTGAGCGCCGAACACATCCTCAATGTCGTGGCGCGCCTGGCCGC
GACCGAACCACCACCCAGCGTCGAAACCCACTTGTCGCTCAAGGAAGCCCCCGTCGCTAACACGGCGCGCTACGACCGCCTGCGTGGCCAGGCCGAGGAG
GTCGGTCATGCGTGATTTGATGGCGGAACTCAAGGAGCTGCGCCTGCACGGCATGGCCACGGCCTGGGCGGAGTTAACTGCACAGGGTGAGTCGAACACA
GCCTCGTCCAAGTGGCTGCTCGAACACCTGCTGGAACAAGAGCACACAGATCGCGCCATGCGCTCGGTGAGCCACCAGATGAACATGGCCAAGCTGCCGA
TGCACCGCGATCTGGCCAGCTTCGATTTCAACGCCTCTAGCGCCGACGCACGCCTGATCAGCGAGCTGGCCAGCCTGGCCTTTACCGACACCGCGCAGAA
CGTGGTGCTGATCGGTGGGCCAGGCACCGGTAAAACCCACCTAGCCACCGCGCTGGCCGTGTCCGGCATCACCCGGCATGGCAAGCGCGTGCGCTTCTAC
TCCACGGTCGATCTGGTCAATCTGCTGGAGCGCGAAAAACACGACGGCAAAGCCGGGCGGATCGCCCAGGCACTGCTGCGCATGGATCTGGTCATCCTCG
ACGAACTGGGTTATCTGCCGTTCAGCCAGGCTGGCGGTGCGTTGCTGTTTCACCTGCTGTCCAAGCTGTACGAACACACCAGCGTGGTGATCACCACCAA
CCTGAGCTTCGCCGAATGGTCGAGCGTGTTCGGCGACGCCAAGATGACCACCGCCCTGCTGGATCGGCTGACCCACCACTGCCACATCGTCGAAACCGGT
AACGAGTCCTATCGCCTGCAACACAGTAGCTTGGCGGCCCAGGCCAAGATCAAATCACGGGAGCGAAAGCGTAAGGGCGGCCAGGAACCGGAGGACGATG
AGTCGTTCTGATTTCATGGTGACGACGGCCTGCTGCATGGCTGCATGTGGCAGGTCTTCAACAGCTGAACTGGGACAGCGAGGGAGTGGGTTCAAAAAGC
AGCTGCGCTGGTAGACTTATCCACAGTTGCTGGTAACAAAATCAGCAATCCGCCCTGGGTCAAATTTCAATCGGCAGGGTGGGTCAATTTTCCATCAGCG
CCAACA
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
1509 bp502 aa1071615+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MISMEMMGKIRRMYFRDKLSLHEIAKRTGLARNTIRKWVRAPEAKQPVYQRRAIFNKLSPFHVTPEQALKADSLRPKQQRRSAKALLAQIKADGYDGGYS
QLTAFIRAWRGGQGKASQAFVPLTFALGEAFQFDWSEEGLLVGGIYRRMQVAHLKLCASRAFWLVAYPSQGHEMLFDAHTRSFGALGGVPRRGIYDNMKT
AVDKVNKGKGRAVNARFAVMCAHYLFDPDFCNVAAGWEKGIVEKNVQDSRRRIWLDAQDCQFHSFEELNAWLGQRCRALWNELTHPQYSGLSVAEVLELE
RAELMPVPAPFDGYVERPARVSSTCLVSVGRNRYSVPCEYAGKWVSSRLYPTRIEVVADDALIASHVRLLDRDQVSYDWQHYLPLIERKPGALRNGAPFA
DLPAPLRQLKHGLGRHAGGDRIMAQVLAAVPVAGLDAVLVAVELVLESGSLSAEHILNVVARLAATEPPPSVETHLSLKEAPVANTARYDRLRGQAEEVG
HA

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
792 bp263 aa16202411+No
ORF function : Accessory Gene
AG : IS21 helper

ORF sequence :

MAELKELRLHGMATAWAELTAQGESNTASSKWLLEHLLEQEHTDRAMRSVSHQMNMAKLPMHRDLASFDFNASSADARLISELASLAFTDTAQNVVLIGG
PGTGKTHLATALAVSGITRHGKRVRFYSTVDLVNLLEREKHDGKAGRIAQALLRMDLVILDELGYLPFSQAGGALLFHLLSKLYEHTSVVITTNLSFAEW
SSVFGDAKMTTALLDRLTHHCHIVETGNESYRLQHSSLAAQAKIKSRERKRKGGQEPEDDESF

 

Blast result :
Comments
ISPst3 is 90% (ORF1) and 94% (ORF2) aa similar to ISPsy20.
References
1] Shintani,M., Nojiri,H., Yoshida,T., Habe,H. and Omori,T.(2002) Direct submission GenBank.