ISfinder Mirror Site

ISRm21

Family ISAs1
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_003047	ND	Sinorhizobium meliloti	Sinorhizobium meliloti 1021

DNA section

IS Length : 1167 bp

Ends

IR Length : 13/16

IRL : CAGGGGAATCGCATTTAGCAAAATAAGCGGTTGTTTTGTTGCGGCAAATC
IRR : CAGGGCTATCGCATATGAGCGATAAGAGAAGTGAGGAATTGGTCATCCCA

Insertion site

Left flank	Direct repeat	Right flank	DR Length
TGACCGCCCC	CCCCACCACC	GGGGCGGCATCGT	10

DNA sequence

CAGGGGAATCGCATTTAGCAAAATAAGCGGTTGTTTTGTTGCGGCAAATCGATTCTTGGAGTTCTCCGCTGACTTGGAGGTCTCGATGAGTAGATTTGCC
GCTTGCTTTGAAGATTTGCCCGATCCGCGCGGTCGCAATGCGCGCCATCCGTTGACGTCGATCCTGTTCATTGCCGTTGCGGCGATTGTCTGCGGTGCGG
AAAGCTGTACCGATATGGCCGATTTCGGCGTTGCCAAGAAGAAATGGCTGAAGACAATCGTGCCGCTGCCCTATGGCATTCCCAGCCATGACACCTTCTC
CACCGTCTTCCGCCATCTCGATCCGGATGCCTTTGACGCGGCCTTTCGCCGTCTCACGGCGAGCTTTGCGCAGGGTCTCGAAGGGGTGGTAGCGATCGAT
GGCAAGGCGGTGCGCGGTGCCTACCGGCGCGCCGCCAAGGCCACGCCACTCCACTTCGTCAATGTCTGGGCTGCCGGTCCCGGTCTGGTCATCGGCCAAA
AGCTCGCGCCGGGCCGCAATGAGGTGCAAGGGGCTCTCGATGCGCTCGCGCTGCTGGCACTGGAGGGCTCTATCGTCACCGCCGATGCCCTGCATTGCCG
GCCCGACACCGCCCGCGCCATCCTCGCTGCCGGCGGCGATTATGCTCTGGCACTGAAGGCCAACCAGCCCGGCCTCTTGGCCCAGGCGCTCGCCCGCATC
GAGGACGCCGACCACGTCGAGAGCATCCAGATTGCAGCCGAGACTGCCCATGACCGCACCGAGACACGCCGCGCCAGCGTTGTGGCTGTCGACGATATCA
ACTTTCCGGGCCTGCAGGCCATCGGCTGCGTCGAGACCACAAGCCGTCATACCAACGGCCATTTGACCAGCCATGTCCGCTACTTCCTGCTCTCCACCAC
CATGTCGCCGAGCGCGCTGATCGAGGTTGTAAGAACCCATTGGCAAATCGAAAACAAACTGCATTGGGTTCTGGATGTCCACTTCCGCGAGGACGCCGCC
AGAAACCGCAAGGACAACGGCCCTCAGAACATTGCCTTCTTGCGCAAGATCGCTCTCAACCTCTTGCGATCTCACCCCGACAAGGCCTCCATCCGCCGGA
AAATCAAAAAGGCGGGCTGGGATGACCAATTCCTCACTTCTCTTATCGCTCATATGCGATAGCCCTG

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
1077 bp	358 aa	86	1162	+	No

ORF function : Transposase
Chemistry : Unknow

ORF sequence :

MSRFAACFEDLPDPRGRNARHPLTSILFIAVAAIVCGAESCTDMADFGVAKKKWLKTIVPLPYGIPSHDTFSTVFRHLDPDAFDAAFRRLTASFAQGLEG
VVAIDGKAVRGAYRRAAKATPLHFVNVWAAGPGLVIGQKLAPGRNEVQGALDALALLALEGSIVTADALHCRPDTARAILAAGGDYALALKANQPGLLAQ
ALARIEDADHVESIQIAAETAHDRTETRRASVVAVDDINFPGLQAIGCVETTSRHTNGHLTSHVRYFLLSTTMSPSALIEVVRTHWQIENKLHWVLDVHF
REDAARNRKDNGPQNIAFLRKIALNLLRSHPDKASIRRKIKKAGWDDQFLTSLIAHMR

Blast result :

Comments

ISRm21 is 57% aa similar to ISPto5.

References

1] Capela,D., Barloy-Hubler,F., Gouzy,J., Bothe,G., Ampe,F., Batut,J., Boistard,P., Becker,A., Boutry,M., Cadieu,E., Dreano,S., Gloux,S., Godrie,T., Goffeau,A., Kahn,D., Kiss,E., Lelaure,V., Masuy,D., Pohl,T., Portetelle,D., Puehler,A., Purnelle,B., Ramsperger,U., Renard,C., Thebault,P., Vandenbol,M., Weidner,S. and Galibert,F. (2001) Proc. Natl. Acad. Sci. U.S.A. 98 (17), 9877-9882