ISSde2
- Family IS481
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_007954 | ND | Shewanella denitrificans | Shewanella denitrificans OS217 |
DNA section
IS Length : 1200 bp
Ends
IR Length : 23/29
IRL : TGTATTAGTCTCGACTTGATCTGACATTCAACTTGAAAAAGTGAGAGTTA
IRR : TGTACTAGCTCAGACTTGATCTGACAGTTACCCATTTTAAATGGTGCCTG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| ATCTAACAAT | CATCAGC | ACTACAATAG | 7 |
DNA sequence
TGTATTAGTCTCGACTTGATCTGACATTCAACTTGAAAAAGTGAGAGTTACCCGTTTTGATAAAGGTGTCGAATCTTTTAATCAAAACCAAAAAGGTAAC
TCTCATGTTTCATACTAACAATCCAATCATTAAACACAAAGCAGGTTTACTCAATTTAGCTGAAGAGCTAGGTAATGTATCTCGAGCATGTAAAGTTATG
GGGGTCTCTAGAGACACATTTTATCGCTATCAAGAGCTTGTTGAAGATGGTGGTATTGACGCTTTAATCGAAAGAACACGCAGAACACCCAATCTAAAAA
ACCGAGTTGATGAAGCAACCGAACAGTCAGTAATTAAATATGCTATCGACTTCCCTGCTCATGGCCAGCATAGAACCAGTAATGAACTACGCAAGCTTGG
TGTTTTTGTATCGGGCAGTGGTGTTCGCTCTATTTGGCTTCGTCATAGCCTAGAGAATTTTAAGAAACGGCTCAAAGCTTTAGAAGATAAGGTCGCTAAT
GACGGGATTATACTCACTGATGCGCAAGTTGCAGCTCTTGAAAAGAAAAAGCATGATGATGAAGCGTGTGGTGAGATAGAAACCCATCATCCTGGGTATC
TTGGTTCTCAAGACACCTTCTATGTCGGCAATTTAAAAGGTGTCGGTAGGATATACCAACAAACCTTTGTTGATACTTACAGCAAGGTAGCATTTGCCAA
GCTTTACACGACTAAAACACCAATCACAGCGGCCGATGTACTTAACGATAAAGTCCTGCCTTATTTCGAGCACCACGAGCTTCCAATGCTTCGAATATTA
ACGGATAGAGGAACAGAATACTGTGGTCGAGTGGAGCATCATGATTACCAGTTATATCTGGCAATTAATGATATCGATCATACAAAAACAAAGGCAATGT
CACCTCAGACCAATGGCATTTGTGAGCGATTTCATAAGACAATTTTAAATGAGTTTTATCAAGTCACTTTCCGCAAAAAGTTATATGACAATTTAGAAGT
GCTGCAAAAAGATCTAGACGAATGGATGAATTACTACAACAATGATCGAACTCATCAAGGGAAAGTGTGTTGCGGAAGAACTCCGCTTGAAACATTGCTT
GATGGGAAACGAATTTGGGCTGAGAAAAATTTAGCTCAAATCTAAACTGACAGGCACCATTTAAAATGGGTAACTGTCAGATCAAGTCTGAGCTAGTACA
TCTCATGTTTCATACTAACAATCCAATCATTAAACACAAAGCAGGTTTACTCAATTTAGCTGAAGAGCTAGGTAATGTATCTCGAGCATGTAAAGTTATG
GGGGTCTCTAGAGACACATTTTATCGCTATCAAGAGCTTGTTGAAGATGGTGGTATTGACGCTTTAATCGAAAGAACACGCAGAACACCCAATCTAAAAA
ACCGAGTTGATGAAGCAACCGAACAGTCAGTAATTAAATATGCTATCGACTTCCCTGCTCATGGCCAGCATAGAACCAGTAATGAACTACGCAAGCTTGG
TGTTTTTGTATCGGGCAGTGGTGTTCGCTCTATTTGGCTTCGTCATAGCCTAGAGAATTTTAAGAAACGGCTCAAAGCTTTAGAAGATAAGGTCGCTAAT
GACGGGATTATACTCACTGATGCGCAAGTTGCAGCTCTTGAAAAGAAAAAGCATGATGATGAAGCGTGTGGTGAGATAGAAACCCATCATCCTGGGTATC
TTGGTTCTCAAGACACCTTCTATGTCGGCAATTTAAAAGGTGTCGGTAGGATATACCAACAAACCTTTGTTGATACTTACAGCAAGGTAGCATTTGCCAA
GCTTTACACGACTAAAACACCAATCACAGCGGCCGATGTACTTAACGATAAAGTCCTGCCTTATTTCGAGCACCACGAGCTTCCAATGCTTCGAATATTA
ACGGATAGAGGAACAGAATACTGTGGTCGAGTGGAGCATCATGATTACCAGTTATATCTGGCAATTAATGATATCGATCATACAAAAACAAAGGCAATGT
CACCTCAGACCAATGGCATTTGTGAGCGATTTCATAAGACAATTTTAAATGAGTTTTATCAAGTCACTTTCCGCAAAAAGTTATATGACAATTTAGAAGT
GCTGCAAAAAGATCTAGACGAATGGATGAATTACTACAACAATGATCGAACTCATCAAGGGAAAGTGTGTTGCGGAAGAACTCCGCTTGAAACATTGCTT
GATGGGAAACGAATTTGGGCTGAGAAAAATTTAGCTCAAATCTAAACTGACAGGCACCATTTAAAATGGGTAACTGTCAGATCAAGTCTGAGCTAGTACA
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1041 bp | 346 aa | 105 | 1145 | + | No |
Chemistry : DDE
ORF sequence :
MFHTNNPIIKHKAGLLNLAEELGNVSRACKVMGVSRDTFYRYQELVEDGGIDALIERTRRTPNLKNRVDEATEQSVIKYAIDFPAHGQHRTSNELRKLGV
FVSGSGVRSIWLRHSLENFKKRLKALEDKVANDGIILTDAQVAALEKKKHDDEACGEIETHHPGYLGSQDTFYVGNLKGVGRIYQQTFVDTYSKVAFAKL
YTTKTPITAADVLNDKVLPYFEHHELPMLRILTDRGTEYCGRVEHHDYQLYLAINDIDHTKTKAMSPQTNGICERFHKTILNEFYQVTFRKKLYDNLEVL
QKDLDEWMNYYNNDRTHQGKVCCGRTPLETLLDGKRIWAEKNLAQI
FVSGSGVRSIWLRHSLENFKKRLKALEDKVANDGIILTDAQVAALEKKKHDDEACGEIETHHPGYLGSQDTFYVGNLKGVGRIYQQTFVDTYSKVAFAKL
YTTKTPITAADVLNDKVLPYFEHHELPMLRILTDRGTEYCGRVEHHDYQLYLAINDIDHTKTKAMSPQTNGICERFHKTILNEFYQVTFRKKLYDNLEVL
QKDLDEWMNYYNNDRTHQGKVCCGRTPLETLLDGKRIWAEKNLAQI
Blast result :
Comments
The ISSde2 transposase has 89% identity with ISVvu4 and the ends of these elements are encoded in are very similar. A single copy of ISSde2 is found in the genome of Shewanella denetrificans.
References
1]Romine, M. F. (2008) Direct submission