ISfinder Mirror Site

IS1421

Family IS5
Group IS427

MGE type ISRelated element(s) :
Isoform Synonym(s) ISRso2

Accession number	Transposition	Origin	Host
NC_003295	ND	Ralstonia solanacearum	Ralstonia solanacearum

DNA section

IS Length : 864 bp

Ends

IR Length : 15/17

IRL : GTGCCGCTAACAAAACCAAGTCATCGAACGCAGGTGGTTGAGCGTTGTTG
IRR : GAGTCGCTAACAAAACCGATCAACAAATCGTCCGCAGATGACGGCGCAAG

Insertion site

Left flank	Direct repeat	Right flank	DR Length
CGACTCGCAT	TTA	GTCAGCAATT	3

DNA sequence

GTGCCGCTAACAAAACCAAGTCATCGAACGCAGGTGGTTGAGCGTTGTTGTTGGCATGGCACGAAAGAAGATCAGCAATGAACTGTGGAAGGCATTGCAA
CCGCTGCTGCCGGTTGTGGAGCCTTCGACCAAAGGCGGTCGTCCGCGCGTGGATGATCGGGCGGCGCTCAACGGCATCCTGTTTGTTCTGCATACCGGTA
TCCCGTGGGAAGACCTGCCTAAAGAACTGGGCTTTGGCAGCGGCATGACGTGCTGGCGTCGCCTGCGGGAGTGGCAGGCCAACGGCGTTTGGGAGCGGCT
GCATTTGGCTCTGCTCAAGCGCCTACGCGAACACGACCAGATCGACTGGAGCCGAGCCAGTGTCGACGGTGCAACGGTGGCCAGCCCCCGGGGGGCGAGC
AGACGGGGCCGAATCCAACGGATCGCGGCAAGCTCGGTAGCAAGCGCCATCTCGTCGTAGATCGGCGCGGCGTGCCGTTGGCGCTGATGGTCACCGGTGC
CAATCGTCACGACTCGGTGGTGTTCGAGGTGCTCGTTGACGCCATCCCGAGCGTGCCCGGACTCGATGGCCGCCCGCGATGCCGCCCCGACAAGCTTCAC
GCGGATAAGGGATACGACTTCGCGCGATGCCGCCGGCATCTGCGCAAGCGGGGCATGACTCCCCGGATCGCTCGCCGTGGCATCGAGAAGAACGACCGGC
TCGGCAAGCATCGCTGGGTGGTCGAACGCACCCATGCCTGGCTTGCTGGATTCGGCAAGTTGCGCATTCGTTTCGAGCGTTCTCTTCAGACTCACCTCGC
TTTGCTCACCCTGGCTTGCGCCGTCATCTGCGGACGATTTGTTGATCGGTTTTGTTAGCGACTC

Recoding section

Recoding by frameshift
Frame
Type
Experimentally demonstrated

Stimulators :

Shine-Dalgarno sequence :
Secondary structure :

Recoding motif :

Protein section

ORF number : 3

ORF 1

Length		Begin	End	Strand	Fusion ORF
405 bp	134 aa	56	460	+	No

ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MARKKISNELWKALQPLLPVVEPSTKGGRPRVDDRAALNGILFVLHTGIPWEDLPKELGFGSGMTCWRRLREWQANGVWERLHLALLKRLREHDQIDWSR
ASVDGATVASPRGASRRGRIQRIAASSVASAISS

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
636 bp	211 aa	223	858	+	No

ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

RTGLWQRHDVLASPAGVAGQRRLGAAAFGSAQAPTRTRPDRLEPSQCRRCNGGQPPGGEQTGPNPTDRGKLGSKRHLVVDRRGVPLALMVTGANRHDSVV
FEVLVDAIPSVPGLDGRPRCRPDKLHADKGYDFARCRRHLRKRGMTPRIARRGIEKNDRLGKHRWVVERTHAWLAGFGKLRIRFERSLQTHLALLTLACA
VICGRFVDRFC

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
803 bp	267 aa	56	858	+	Yes

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MARKKISNELWKALQPLLPVVEPSTKGGRPRVDDRAALNGILFVLHTGIPWEDLPKELGFGSGMTCWRRLREWQANGVWERLHLALLKRLREHDQIDWSR
ASVDGATVASPPGGEQTGPNPTDRGKLGSKRHLVVDRRGVPLALMVTGANRHDSVVFEVLVDAIPSVPGLDGRPRCRPDKLHADKGYDFARCRRHLRKRG
MTPRIARRGIEKNDRLGKHRWVVERTHAWLAGFGKLRIRFERSLQTHLALLTLACAVICGRFVDRFC

Blast result :

Comments

There are 6 copies of IS1421 on Ralstonia solanacearum genome (1 on chromosome and 5 on megaplasmid).The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.

References

1] Salanoubat,M., Genin,S., Artiguenave,F., Gouzy,J., Mangenot,S.,Arlat,M., Billault,A., Brottier,P., Camus,J.C., Cattolico,L., Chandler,M., Choisne,N., Claudel-Renard,C., Cunnac,S., Demange,N., Gaspin,C., Lavie,M., Moisan,A., Robert,C., Saurin,W., Schiex,T., Siguier,P., Thebault,P., Whalen,M., Wincker,P., Levy,M., Weissenbach,J. and Boucher,C.A. (2002) Nature 415 (6871), 497-502