ISfinder Mirror Site

ISSme2

Family IS21
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_009621	ND	Sinorhizobium medicae	Sinorhizobium medicae WSM419 plasmid pSMED02

DNA section

IS Length : 2219 bp

Ends

IR Length : 21/29

IRL : TGTCGGGCGAAATGAAAAACTGACCCCCTAACGAAGCGAAGAACTGACCC
IRR : TGTCAAGCGAATCGAGGAATTGACCCCTTTGGCGAATTGAAGAACTGACC

Insertion site

Left flank	Direct repeat	Right flank	DR Length
AATAGCGGATTC	TCTGC	CCACCCGATGTC	5

DNA sequence

TGTCGGGCGAAATGAAAAACTGACCCCCTAACGAAGCGAAGAACTGACCCCCTCGTGTCAAAACGAGGAGGAATGGATGACGAGCGCGATTTTATCGCAT
CCTGCATTGTCGCTAACGATGCAGGGCGGGGATATGCTTCAGGCTGACGAGGTGGTGGCAATGCTGCGGCTGCACGAGCTTGGTTGGGGTAGCAAACGTC
TATCGAAAGAATTTGGATGCGCGCGGAATACCGTTCGCCGATATCTTCGAGAGGGCGGAGCTGTACCGTTTAAACAGCCTGCCCGGCGCAGTGCGTTCGA
CGGGCTTGATGATTGGCTTCGCGAGCGTTTTTTCCGGCATGACGGTAATGCGGATGTGATCCGCCAAGAGTTGGCGAGCGAGCACGGAATTGTCATCGGT
CTGCGTTCTGTGGAGCTCCGCGTACGGCAGTGGCGGCGAGAGCTAAAGGCACAGAAGCGGGCGACGGTCCGCTTCGAGACGGCGCCGGGCCATCAGATGC
AGATCGACTTCGGTGACACGAAGGTGTGGATCGGCGACGAGCGGGTTCGGATTCACCTGTTCGTGGGGACGCTGGGGTATTCGCGGCGGATGCATGCTCG
CGCGTCACTCAGGGAGCGCCAGGCAGACTGGTTTGAAGGCATGGAAGGCGCTTTCCTGCGGTTCGGCGGGGTTCCGGTGGAAGTGCTGATCGACAATGCG
AAGGCCCTGGTCGAACATCATGATCCGGTGACGCGAGAGGTGAGATTCAACGCGCGACTGCATGCTTTCGCCCGTTATTGGGGCTTCACGCCGCGGGCCT
GCGCACCGTATCGGGCGAGAACGAAAGGCAAAGACGAGCGCGGGGTCGGTTACGTCAAGAAGAACGCGATCGCCGGGCGCCGCTTCGAGAGCTGGGCCGA
GTTTGAAGCGCATCTGGATCGATGGACACGCGAAGTTGCCGACCAGCGTGAACACGGCACCACCGGTGTCAAACCGGCGGAACGCTTTGCCGACGAAGCC
AGGGAGCTGCGCCCGCTGGCCGGACGGGCACCCTTCGGGCAATTGCGGGATCTGGTTCGCAAGGTTCAAGCCGATTGCGCGATCGACCTCGACACCAACA
GCTACTCGGTGCCCTGGCGCCTGATCGGCGAGAGTGTTCAGGTCGTGGTGTTGGCGGGGCGCGTCATCATCCGGCATGCGGGCCAGGTGGTGGCTGATCA
TGCCCTGTGCGATGGACGACGACAACGGATCGTGGACCGGGCGCATTTTGTCGGTGTTGCCGGCGCCGAGGGTCTGGTGCGAGCCGCCGCTCCCATCGAG
CTCCCCCCTCCCACCCTGTTGCGCCCGCTTGCGGAATACGAGGCGGTTGCCGGAGGAGGCTGGTGATGACGAACGTGGATCATGACGTGCTGATCGCAAC
GCTCGATCGGCTGAAGCTGACGGCGATCCGCGACCAGCTCGACACGTTGCTGGACGAGGCGGCTCGTTCGAAGATGACCTTGCGCGAGGCGCTGGCTTTC
CTTGTGTCGCGCGAGATTGCCCGGCGCGACGAGCGGCGGATATCGATGTCGAGCAAGCTGGCGCAGTTCCCGTTCGTGCGCGAATTGGACGGCTTCGACT
TCGAGGCGCAGCCCTCGCTGGATCAGGGGCAGATCAGGGAGCTTGCGACCTGCCGCTGGATCGCTCACGGTGATACCGTACTCTTCCTTGGACCGCCCGG
TACAGGCAAGACGCATCTGGCTGTGGGCCTCGGCCGCGAGGCCATCCGCCAGAATTACAACGTCCAGTTCGTCACGGCGGCCACGCTAGTGGCGATGCTG
GCGAAGGCTCACAACGACGGCTCGCTCGACAAACAGTTGACGATCCTGTCGCGGCCGAAATTGCTGATCATCGACGAGCTGGGTTACTTGCCCTTCGAGG
CCAATGCCGCCCACCTGTTCTTCCAACTGGTGTCTCGGCGCTACGAGAAGGGCTCGATCCTGATCACCTCAAACCGTTCCGTGGGCGAATGGGGAAGTGT
TTTTGGCGACCCTGTCGTCGCCACGGCGATATTGGACCGCCTGCTTCACCACTCGACGGTGATCACCATCCGGGGCGACAGCTATCGGCTTCGCGAAAAG
CGCCGCTCCGGCCTTCTGCAGAAAGCCGGAGCGGCGCTCCAACCCAACGAAACCGCAAACCAATGAGGGGGTCAGTTCTTCAATTCGCCAAAGGGGTCAA
TTCCTCGATTCGCTTGACA

Protein section

ORF number : 2

ORF 1

Length		Begin	End	Strand	Fusion ORF
1290 bp	429 aa	77	1366	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MTSAILSHPALSLTMQGGDMLQADEVVAMLRLHELGWGSKRLSKEFGCARNTVRRYLREGGAVPFKQPARRSAFDGLDDWLRERFFRHDGNADVIRQELA
SEHGIVIGLRSVELRVRQWRRELKAQKRATVRFETAPGHQMQIDFGDTKVWIGDERVRIHLFVGTLGYSRRMHARASLRERQADWFEGMEGAFLRFGGVP
VEVLIDNAKALVEHHDPVTREVRFNARLHAFARYWGFTPRACAPYRARTKGKDERGVGYVKKNAIAGRRFESWAEFEAHLDRWTREVADQREHGTTGVKP
AERFADEARELRPLAGRAPFGQLRDLVRKVQADCAIDLDTNSYSVPWRLIGESVQVVVLAGRVIIRHAGQVVADHALCDGRRQRIVDRAHFVGVAGAEGL
VRAAAPIELPPPTLLRPLAEYEAVAGGGW

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
801 bp	266 aa	1366	2166	+	No

ORF function : Accessory Gene
AG : IS21 helper

ORF sequence :

MTNVDHDVLIATLDRLKLTAIRDQLDTLLDEAARSKMTLREALAFLVSREIARRDERRISMSSKLAQFPFVRELDGFDFEAQPSLDQGQIRELATCRWIA
HGDTVLFLGPPGTGKTHLAVGLGREAIRQNYNVQFVTAATLVAMLAKAHNDGSLDKQLTILSRPKLLIIDELGYLPFEANAAHLFFQLVSRRYEKGSILI
TSNRSVGEWGSVFGDPVVATAILDRLLHHSTVITIRGDSYRLREKRRSGLLQKAGAALQPNETANQ

Blast result :

Comments

ISSme2 is 52%(ORF1) aa similar to IS21 and 64%(ORF2) aa similar to ISMac3.
L1 : AACTGACCCCCT
L2 : CGAAGCGAAGAACTGACCCCCT
R1 : AATTGACCCCTT
R2 : CGAATTGAAGAACTGACCCCC

References

1] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Glavina del Rio,T., Dalin,E., Tice,H., Pitluck,S., Chain,P., Malfatti,S., Shin,M., Vergez,L., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Mikhailova,N., Reeve,W. and Richardson,P. (2007) Direct Submission GenBank.