ISBce16

  • Family IS21
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
DQ889676 ND Bacillus cereus
Bacillus cereus AH187 plasmid pCER270
DNA section
IS Length : 2160 bp

Ends


IR Length : 35/50

IRL : TGAAAACAACGGTGGTAAAATCCCTAATAACAACGGTGTGAAATTCCCCA
IRR : TGTCAACAGCGGTTCTATTTTCCCTAATTTCAGCGGTCCAAAATTCCCTA

Insertion site


Left flankDirect repeatRight flankDR Length
CAATAATGATTTACTTCTCAATACTAATTTATCTAT4

DNA sequence

TGAAAACAACGGTGGTAAAATCCCTAATAACAACGGTGTGAAATTCCCCAATAATAACGGATAATTATCTCAAAAACAACTTGGAGTGTAGAGAGATAAT
GATCAAGGATGGGGAATTTTTTATGATTAAAGAGTATCACTCAAAAGGTTGGACTCAGATTGCTATAGCAGAATTAACAGGCTTTGATCCAAAAACAATA
CGAAAATACTTAAATCAAAATGAGTTACCAAAGAAAAAAAAACAACCTCACGGTAAATTGAAAACAAGCCTTTTAGATCCATATAAAGACTATTTATTAA
GACGGTTAAAGGAAGGTACCACTAACTGCGAAGTGCTTCTTGAGGAAATTCAATCTATGGGTTATCCAGGAAAGATGACTATTTTAAGAGATTTCATCAG
ACCTTATCGCCAGAAACCAAAGAAACAATCGAGTATCCGTTTTGAAACACCTCCTGGAAAGCAAGCACAGATGGACTGGGCAGAGATTGGAGAACATGAG
GTTGATGGGAAGAAACAAAAGCTTTATGTATTTATCATCGTTTTAGGTTACTCCCGTATGAAATACATGGAAATTACAAATACGATGAATATGGAGCAGC
TAATGAAATGCCACATGAATGCATTTGCCTATTTTAACGGCGTTCCTGAACACATACTCTATGACAATATGAAAACGGTAGTCACCTTACATAGTCCAAC
ACAAATACGTTTTAATCGAAAGTTCGAAGAATTCCTAGCTTACTATGGAGTTGTTCCGAAAGCGTGTAAACCAGCAAGACCACAAACCAAAGGGAAAGTA
GAAAGTGCGGTTGGTTACCTTAAGAAGAATTTTCTGCAAAGAAAACAAAAGAATACACTTCATGAGATGAATGAGATGTTAAGTGGATGGCTGGACAAAG
TGGCCAATAAAAAACCGAATCAAACGACGCTTGAGCCACCTCTACAGCGTTTTGAAGAGGAACAAAAACACTTATTACATTGGAATACGAGACCCTTATT
CCCAATAAGTAAATGGAAGGTTACTTACGTAACGGAAAGCAGCATGATTTCTTATAATGATCGAAACTATTCGGTTCCTTATCGTTATAAAGGGATGGAA
GTAAAGGTGAAGGAAACTCTTGATCATCATTTAGAGATCTATTTTGATCTAGAGTGCATCGCTACTCATCCGATTATTTCTGGGATCAATAAATCATTAA
CGGATATCAACCACATAAAGAACATAAAAAAAGAGCCAGATGTGACACAAAACGGTTTGGCGACCCGTCACATTCAGCCCTCTAATCATGAAGTGGAACA
ACGTTCACTTCACGTGTATGAAGAACAATTAAAAGCTGGTGATCACAAATGAACGCACAACTTATAACTGAACGTCTTAAGAAAGTTGGCTTGGTTTACA
TAGCCAATCAATTAGATGGCATCCTAGAAGATGCCTCTAAACATAATGTAACATATTCTGAATTTCTAAACACCCTTCTTCAACATGAAATTGAACACAA
AGAAGGTGCAGCACTAGAAACTAGAATAAAGAAATCAAAGCTACCGTTTCACAAGACTATCGAAGACTTTGACTTCTCATTTCAACCAAGTGTTAGCGAA
CGGCGTGTAAAAGAAGTAATAACTGGTCGTTATATTGAGAATGGAGAAAATATCTTATTATTAGGACCGCCAGGTGTTGGTAAAACCCACTTGGCGATAG
GTTTCGCTATCGAAGCACTTCGTCAAGGATACACAGCTTACTACACCCGGGCCGATGATTTCATTACTTCCTGCCGCAAGGCAGAACAAAGGGGAACCAC
TTCTCGCCTGGTCCGATCGTTAAGTAGGTGCGATGTACTCATAATTGATGAAATGGGATACTTTCCGTTTGATGAGTTAAGTGCAGATTTATTCTTTCGT
ATCATTTCAAAACGTTATGAAAAAGGGTCAATTATTTTAACCTCTAACAAATCGTATATTGAGTGGGGGAAAATATTTGGCGATGACGTCCTAGCAACAG
CGGTTTTGGATAGATTGTTGCATTACTCCACAACTTTCAATATCAAAGGACAATCATTTAGACTTCGTGAAAAGCAAAAAGCCGGCATACAACCGGCCGT
TAATTAGTAATAGGGAATTTTGGACCGCTGAAATTAGGGAAAATAGAACCGCTGTTGACA
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
1254 bp417 aa991352+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MIKDGEFFMIKEYHSKGWTQIAIAELTGFDPKTIRKYLNQNELPKKKKQPHGKLKTSLLDPYKDYLLRRLKEGTTNCEVLLEEIQSMGYPGKMTILRDFI
RPYRQKPKKQSSIRFETPPGKQAQMDWAEIGEHEVDGKKQKLYVFIIVLGYSRMKYMEITNTMNMEQLMKCHMNAFAYFNGVPEHILYDNMKTVVTLHSP
TQIRFNRKFEEFLAYYGVVPKACKPARPQTKGKVESAVGYLKKNFLQRKQKNTLHEMNEMLSGWLDKVANKKPNQTTLEPPLQRFEEEQKHLLHWNTRPL
FPISKWKVTYVTESSMISYNDRNYSVPYRYKGMEVKVKETLDHHLEIYFDLECIATHPIISGINKSLTDINHIKNIKKEPDVTQNGLATRHIQPSNHEVE
QRSLHVYEEQLKAGDHK

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
759 bp252 aa13492107+No
ORF function : Accessory Gene
AG : IS21 helper

ORF sequence :

MNAQLITERLKKVGLVYIANQLDGILEDASKHNVTYSEFLNTLLQHEIEHKEGAALETRIKKSKLPFHKTIEDFDFSFQPSVSERRVKEVITGRYIENGE
NILLLGPPGVGKTHLAIGFAIEALRQGYTAYYTRADDFITSCRKAEQRGTTSRLVRSLSRCDVLIIDEMGYFPFDELSADLFFRIISKRYEKGSIILTSN
KSYIEWGKIFGDDVLATAVLDRLLHYSTTFNIKGQSFRLREKQKAGIQPAVN

 

Blast result :
Comments
ISBce16 is 60% (ORF1) aa similar to ISMac9 and 70% (ORF2) aa similar to IS5376.

L1 : TCCCTAAT
L2 : TCCCCAA
R1 : TCCCTAAT
R2 : TCCCTA
References
1] Rasko,D.A., Rosovitz,M.J., Okstad,O.A., Fouts,D.E., Jiang,L., Cer,R.Z., Kolsto,A.B., Gill,S.R. and Ravel,J. (2007) J. Bacteriol. 189 (1), 52-64.

2] ISfinder annotation (2008).