ISChy4

  • Family IS21
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_007503 ND Carboxydothermus hydrogenoformans
Carboxydothermus hydrogenoformans Z-2901
DNA section
IS Length : 2172 bp

Ends


IR Length : 19/27

IRL : TGTCACCGCCGGTATATAATTGACCCAGGAACAACGAAAAGGAAATGACC
IRR : TGTCAATAGCGGATTTAAATTGACCCATTTTCAGCGGTTTTAAATTGACC

Insertion site


Left flankDirect repeatRight flankDR Length
TGCAAAAGTTAAGTTGATATTTAATCCGATATGTTTTATTA7

DNA sequence

TGTCACCGCCGGTATATAATTGACCCAGGAACAACGAAAAGGAAATGACCCACCCCCTGGCGAAATATCCCTCTGATACCACTGCAAAGGATCGGAGGGA
AATAAATGCTAGGGAGTGGATCTATTATCATGTTACATGAATTACAAGCAAGAGGCAAGAGTATTCGTGCAATCGCACGAGAAACAGGGCATTCCAGAAA
TACGGTAAGAAAATACCTCAGAGCAGAGGGCATTCCTGAAAGGAAGCCCCGTCCCAAAAGAGGTTCAAAGCTTGACCCCTACAAAGATACTATTCAAGAG
CTCATGAATCTGGGGATATTCAATTGCGAAGTCATTTATGAAAGAATCAAGGAAGAAGGCTACACCGGAGGTCGCACTATTTTAAGGGACTATGTAAGAC
AATTTAGACCTCCAAAACAGGTCCCTGCCGTATGCCGCTATGAAACCAAGCCTGGCCAGCAAGCCCAGGTCGACTGGGGCGAATACACCTACATTGACGA
GGAAACCGGTGAAATACGTAAGCTTTACGTCTTCGTCATGGTGCTGGGCTACTCCAGAGCCATATACGTGGAATTCACAAACCGCTGCGACGTCCGTACC
TTCATCCGCTGCTTGATCCACGGGTTTGAATACTTCGGCGGAGTAACCGATATAGTTCTTACCGATAGAATGAAAACCGTAATACTGGGCACCGGTGAAA
ACAAAAAGCCCATATGGAACCCTACCTTTGAAGACCTTGCGGCGACCCTTGGATTTATTTCTAAAGTGTGCAGGGCACGGCGCCCCCAAACAAAAGGCAA
GGTAGAAAGCGGCATAGATTTTGTCAAAAACAACTTCCTGCCGGGTAGGAAGTTCGTAGACTACGGTGATTTAAACCGCCAGGCAATAGTGTGGTGCGAA
AAGAAAAACAGGAGAATACACGGTACTACCGGGGAAAGGCCTATTGACCGTCTGAAGAAGGAAAACCTCAAACCCCTGCCTGCCCTTGATAAATACCAAA
AATTCCTGGAAGAAGCAAGGAAAGTTCACAAAGACGGCTTTTTGAGTTTCGACGGCGTAAGATATGGCGTTCCCTGGCAGTATAGCGGAAAAGAGGTGGT
TGTAAGGGACAAAAACGGTAAAATCGAAATCCTCTATGATGGGAAGGTGATAGCTGTCCACGAAAAGCATTACCGTTCAAGGAGCACCGTTTTTCTCAAA
GACCAGTATAAGGGTCTAAAAGAAGCGGAGGGCATGTTTTATCCCAGGCCGAAGGCGATCAAGTTATCTTCCCTGGAAGTTGAGAAGCGTCCTCTGGGGG
TTTACGAAAGCCTCCTGGAGGTAGGCACAGTATGATAGACCTTGAAAAAGCTCGGTCCCACCTTGAAGAACTTGGGCTTTTAAGCGCAGCAGCTTTTCTT
GACGCCCTCCTGGAAAGGGCCCAGCGGGAAAACCGCACATATCTTGATTTCTTAAATGATCTGCTTGAAACTGAACTTGCCGAAAGGCAAAGGCGAAATG
TCGAGGTAAGGTCAAAACTTGCCCGGCTTCCGTATAAAAAGACCCTGAAGGAATTTGACTTTACCTTCCAGCCCAGTATCGATGAAAAACTGATAAGAGA
GCTTGCCACAATGGCCTTTGTCCACCGGGCAGAAAACGTAATATTCCTTGGGCCGCCTGGAGTAGGGAAGACGCACCTTGCCGTAGCCCTTGCTATAGAA
GCCCTATCCCAGGGCATATCAGTTTACTTTACGAGCCTTTCCAGGCTCATTGAAGACCTAAAAACAGCCCATAAAGAAAGCCGGTTGGAAAGGCGAATGA
GGATCTACCTTAGGCCCAAGCTCCTTATTATCGACGAAGTGGGCTATCTCCCTTTAGATGGCCTTGGCTCAAACCTCTTTTTCCAGCTAATTAGTGCCCG
GTATGAAAAGGGGAGCATCATCCTCACCAGCAACAAAAGCTTTGGGGAATGGGGGGAGCTCATGGGAGACCCGGTGCTTGCCACTGCAGTGTTGGATAGG
CTATTACACCATGCCCATATAATCAACATAAGGGGCAACAGCTACCGCCTAAAAGACAGGTTAAAAACCGGTCTCTACGGTAATCCACATGTCAAAGCTT
AATTTTAAAAAAAGCCAGGGTGGGTCAATTTAAAACCGCTGAAAATGGGTCAATTTAAATCCGCTATTGACA
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
1230 bp409 aa1061335+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MLGSGSIIMLHELQARGKSIRAIARETGHSRNTVRKYLRAEGIPERKPRPKRGSKLDPYKDTIQELMNLGIFNCEVIYERIKEEGYTGGRTILRDYVRQF
RPPKQVPAVCRYETKPGQQAQVDWGEYTYIDEETGEIRKLYVFVMVLGYSRAIYVEFTNRCDVRTFIRCLIHGFEYFGGVTDIVLTDRMKTVILGTGENK
KPIWNPTFEDLAATLGFISKVCRARRPQTKGKVESGIDFVKNNFLPGRKFVDYGDLNRQAIVWCEKKNRRIHGTTGERPIDRLKKENLKPLPALDKYQKF
LEEARKVHKDGFLSFDGVRYGVPWQYSGKEVVVRDKNGKIEILYDGKVIAVHEKHYRSRSTVFLKDQYKGLKEAEGMFYPRPKAIKLSSLEVEKRPLGVY
ESLLEVGTV

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
771 bp256 aa13322102+No
ORF function : Accessory Gene
AG : IS21 helper

ORF sequence :

MIDLEKARSHLEELGLLSAAAFLDALLERAQRENRTYLDFLNDLLETELAERQRRNVEVRSKLARLPYKKTLKEFDFTFQPSIDEKLIRELATMAFVHRA
ENVIFLGPPGVGKTHLAVALAIEALSQGISVYFTSLSRLIEDLKTAHKESRLERRMRIYLRPKLLIIDEVGYLPLDGLGSNLFFQLISARYEKGSIILTS
NKSFGEWGELMGDPVLATAVLDRLLHHAHIINIRGNSYRLKDRLKTGLYGNPHVKA

 

Blast result :
Comments
ISChy4 is 60% (ORF1) and 72% (ORF2) aa similar to ISMac3.
L1: TGACCCACCC
R1: TGACCCA
R2: TGACCCACCC
References
1] Wu,M., Ren,Q., Durkin,A.S., Daugherty,S.C., Brinkac,L.M., Dodson,R.J., Madupu,R., Sullivan,S.A., Kolonay,J.F., Nelson,W.C., Tallon,L.J., Jones,K.M., Ulrich,L.E., Gonzalez,J.M., Zhulin,I.B., Robb,F.T. and Eisen,J.A. (2005) PLoS Genet. 1 (5), E65.