ISBcen28

  • Family IS21
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
CH482377 ND Burkholderia cenocepacia
Burkholderia cenocepacia PC184
DNA section
IS Length : 2311 bp

Ends


IR Length : 35/50

IRL : TGTCAGCATCGGATAGATACTCCCCACTTTCGTCGAAGTAATTTTCCCCA
IRR : TGTCAACGTCGGACGTAATTTCCCCAGAAGTGTCGAAGTAAAATTCCCCA

Insertion site


Left flankDirect repeatRight flankDR Length
CGATGGTAGAAATAGGAGCTGCGCGCCAGGTCC5

DNA sequence

TGTCAGCATCGGATAGATACTCCCCACTTTCGTCGAAGTAATTTTCCCCAGTTTTTTGTCTGACGGCGCCGCGGTAGCGGCGCCGTCCTGTCGATCGCCG
ACCATATCGCCCTGGAGATCCGTCGTGGGGGTATCGGTGGTCGAGCTGGAGGAAGTAATGACGATTCTGGAATTGCATCGGCAAGGCCTGAGTATTTCGG
CCATCGCTGCTCGACTGGGTATGGACCGCAAGACCGTTCGTAAATACATCAGGAATGGCGTTCAGGCGCCACGTTACGGTCCGCGCGCACCGCGACCGTG
CGTGGTCGATCCGTTCGTCCACTACGTCACCGAACGTGTGCGCGAGTTTCCGGACCTGAGCGTCGAGCGTCTGCTGCGCGAGATTCGGGCCATGGGGTAT
ACCGGTGGCCGCACGGCGCTGGGCGATCTGGTTCGAGAGGTCCGGCCACCCCGCCAACGCGGTTTCGAGGTGCGCTTCGAGACGCCTGCCGGGCATCAAG
CGCAGGTGGATTTCGCCCACTTCAATGTCGAGTTCGGCGACGCGCCCGGCCTGCGACGTTCGATCTGGCTGTTCTCGATCGTGCTTGGACACAGCCGGTA
TCTCTGGGGACGTTTCGTCGAGCATCAGGACTTGCAGACGGTCCTGCGCTGCCACATGGAAGCGTTCGAGCATCTCGGCGGCGCTCCGCGCGAGATTCTG
TATGACCGGATGAAGGCGGCCGTGCTGGGCGAAGTCGAGAAGCACATCGTCTACAACGCGAAGCTGGTCGCGTTTGCGCAGCACTACGGCTTCGCGCCGC
GTGCCTGCAAAGCGTATCGAGCCAAGACCAAGGGCAAGGTCGAACGGCCGTATCGCTATATCCGGCAGGACTTCTTCCTGGCGCGCCGTTTCCAGAATCT
CGCCGACCTGAACCGACAGTTGCGCGAGTGGCTCGACACGGTGGCCAATGTGCGCATGCACGGCACGACGCACCGCGTCGTCGCCCAGCACTTCGGTGAG
GAGCGCCCCGCACTGCAGGCGTTGCCGGCCAGCACCTTCAACGGCGTGATCCGGCTCGAGCGTCGCGTGAGTCACGAAGGCCTCGTCTCGGTCGGCGGCA
ACTACTACAGCGTGCCCGATCGAACACGCAAGCGCACGCTCGACGTCCACAGTCTGGCCCACGAGATCCGCATCTACGAAGACGGTGAACTGCTTGCGGT
TCATCCCGTGCTCGAAGGCCGGCGACGAACCTCGCTGTTGCCCGGCCATCGGCGCGCAAATCAGCGCAAGCAGCCAGCACGACACCCATCACCATCGGTA
ACGGTAGCCGTAGCTCGCCGGCCTCTGTCGTTCTATGACCAGGTCGCAAGACGCCTCGCAGATGCCGGGAGGACCGCATGAGCAGCATGCCCGCCTCGAC
ACTGGAACGCATTCGACGCTATCTCGTCGGCTTGCGCATGCCGCGCGCGCTCGAAACGCTGGACGCCACCCTGAATCGTTTCGAGCAGGGCGACAGCTCA
ATGCTGGAAGTGTTGGAAACGCTGCTGGGCGAGGAGTTCACGACGCGCGAGACCCGCCGCATCCGGATGGCGCTGCAGACCGCGCGACTCGGCACGATCA
AGACGCTGGCTGGCTACGACTTCAGCTTCCAGCCGAGTCTCGACCGAGATCGCATCATGACGCTTGCGCAACTCGAGTTCATCGAACGACGACAGACCGT
ACACTTCCTGGGGCCGCCCGGCACGGGCAAATCGCACCTGTCCATTGCGCTGGGCGTCGAAGCCGTGCGCGCCGGCAAGAGCGTCTACTTCGGCTCGCTG
GCCGAGATCGTGAACTCGATGGCCAAGGCCGAGCGGGAAGGCAACCTGGCGCAGCGCGTGCGCTTCCTTGCCCGCAACAGCCTGCTCATCGTCGACGAGA
TTGGTTACCTGCCCATCGGCTCCAACGGCGGCAATCTGTTCTTCCAACTCGTCAACGCCTGCTACGAGCGCTGCGCGATCATCCTGACGTCCAATCGCAG
CTTCGGCGAATGGGGTGAAGTGTTCGGCGACAGCGTGGTCGCCGCGGCGCTGCTCGACCGGCTGCTGCACCACGCGATCGTCGTGCAGATCGAGGGCACG
TCGTACCGTCTGCGAGAACACGCCGATCTGCTGCCCGACCATCTGCGCAACCGACCGTCTTCCCTGAATCACACGCCCGCTGAACCGGTTCGTCGGCGCC
CGGGCCGTCCGCGAAGGAGCTCATCCGATCACCTCGCCGGCTGATCACCGTCCGAACAGGGTGGGGAATTTTACTTCGACACTTCTGGGGAAATTACGTC
CGACGTTGACA
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
1257 bp418 aa1251381+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MGVSVVELEEVMTILELHRQGLSISAIAARLGMDRKTVRKYIRNGVQAPRYGPRAPRPCVVDPFVHYVTERVREFPDLSVERLLREIRAMGYTGGRTALG
DLVREVRPPRQRGFEVRFETPAGHQAQVDFAHFNVEFGDAPGLRRSIWLFSIVLGHSRYLWGRFVEHQDLQTVLRCHMEAFEHLGGAPREILYDRMKAAV
LGEVEKHIVYNAKLVAFAQHYGFAPRACKAYRAKTKGKVERPYRYIRQDFFLARRFQNLADLNRQLREWLDTVANVRMHGTTHRVVAQHFGEERPALQAL
PASTFNGVIRLERRVSHEGLVSVGGNYYSVPDRTRKRTLDVHSLAHEIRIYEDGELLAVHPVLEGRRRTSLLPGHRRANQRKQPARHPSPSVTVAVARRP
LSFYDQVARRLADAGRTA

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
867 bp288 aa13782244+No
ORF function : Accessory Gene
AG : IS21 helper

ORF sequence :

MSSMPASTLERIRRYLVGLRMPRALETLDATLNRFEQGDSSMLEVLETLLGEEFTTRETRRIRMALQTARLGTIKTLAGYDFSFQPSLDRDRIMTLAQLE
FIERRQTVHFLGPPGTGKSHLSIALGVEAVRAGKSVYFGSLAEIVNSMAKAEREGNLAQRVRFLARNSLLIVDEIGYLPIGSNGGNLFFQLVNACYERCA
IILTSNRSFGEWGEVFGDSVVAAALLDRLLHHAIVVQIEGTSYRLREHADLLPDHLRNRPSSLNHTPAEPVRRRPGRPRRSSSDHLAG

 

Blast result :
Comments
ISBcen28 is 58%(ORF1) and 63%(ORFB) aa similar to ISMac3.
L1: TTTCCCCAG
R1: TTTCCCCAG
R2: TTCCCCA
References
1] Birren,B., Lander,E., Galagan,J., Devon,K., Nusbaum,C., Borowsky,M.L., Jaffe,D., Butler,J., Alvarez,P., Gnerre,S., Grabherr,M., Kleber,M., Mauceli,E., Brockman,W., Rounsley,S., Young,S., LaButti,K., Pushparaj,V., DeCaprio,D., Crawford,M., Koehrsen,M., Engels,R., Montgomery,P., Pearson,M., Howarth,C., Kodira,C., Zeng,Q., Yandava,C., Oleary,S., Alvarado,L., Lory,S. and LiPuma,J. (2005) Direct Submission GenBank.

2] ISfinder annotation (2008).