ISPepr5
- Family IS21
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_008607 | ND | Pelobacter propionicus | Pelobacter propionicus DSM 2379 plasmid pPRO1 |
DNA section
IS Length : 2193 bp
Ends
IR Length : 24/37
IRL : TGTCAATGGTAGTGCAATAATGTCCCAAAAGCGGTAACGAAAAGTGTACC
IRR : TGTCAATGGGGACGTAAAAGTGTACCAGTTTCGGGAATTGAAAAGTGTAC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CATATTTAGGGTTGACTTG | GCCGG | TGCGTTCAGCATGCCCAC | 5 |
DNA sequence
TGTCAATGGTAGTGCAATAATGTCCCAAAAGCGGTAACGAAAAGTGTACCACCCTGGAAGCAGTGGTATCCCTTCTTATGAAAGGAGGGGTGCCCATGAT
TTCCACGGAGGTAGTTATGGACATAGCCGCACTCAAACGCTCCGGCCACAGTATCCGATGGATCGCACGCAAGCTGGGCATTCATCGCAAGACTGTGAAG
AAGTATCTCGAAAGCAACTCTTTCCCCGCATATCACCGGAAAGACACCAAACCATCCATCCTTGAACCGTATCACCAGATCATCCGGGATTTTCTCGATG
AGGATGAATATCAGGCCACCTGGATACTCACCCGCATCAAAAGGATGGGGTATTCCGGCAGTTACGATACCCTCAAGGTATTCGTCCGCTCCATCAAGGA
ACAGAAGCGTCGCATTGCTTATCAGCGCTTCGAAACGGAGCCCGGTCTCCAGGCCCAGGTTGACTGGGGAGATTTCAAGGTGGTCGATTCCACGGGAAAG
ACCACACCCCTTTACGCCTTCGTAATGGTCCTGGGATTTTCCCGGGCCATGTACGTCGAATTCGTTGAACGATGCACCCTGGAGGCGTTCATGGACTGTC
ACCTCCGGGCATTTCGCCACCTCAAGGGCGTTCCCGCCGAAATCCTCTACGACAACATGAAGCAGGTCGTCGTGGGCCGGGAGAATGGCCGGCCGGTGTT
CAACACCGAATTCCTCCATTTTGCCAGGCACTACGGTTTTACGCCCCGCCTCTGCCCTCCCTACAGCCCCTGGGTAAAGGGAAAGGTCGAACGCCCCATG
CACTATGTGCGGGAAAGCTTCTGGCGGGGGTATGGATTCCATTCTCTGGAAGAGACCAATCAGGATGTTGCTGCCTGGATCGCGGAGACCGCCCACCAGC
GTATCCACGGTACCCACCGTCAGCAGGTTCAGGCTCGCTGGGAGCAGGAACAGCCCTGCCTGGGAGCGCTACCGCCCTCCGAGTACGACACATCCCTCAA
GTATTTTCGCAAGGTCTATAAGGACTGCCTGCTCTCCTTTGGCGGCAACCGCTACTACGTTCCCTACCAGGCCGCGGGGAGAAAGGTCCTTTTGAAGGTC
AAGGCCAGCGTCATTCGGATCTATCACGATGACGAACTGCTGGCCCTGTACCAGATTCCGGAGATCAACGGGCAGACCATCGGCATTCCTGACAAACCGC
CACCCCGGACTCCCCGGCAGACCCCGCGCTATGGTCAGGACCGGGGGATGGCCACCCGTGGTCTCACGACCGGAACGCTCTACCCCGAAGTCTACCGTCG
CCCTCTGGAAGAGTATGACCGCTACGCCGCCGGAGGTGCCCCATGGAACAGCTGACCTATGAGCGCCTCCAGGACAACCTGAAACGGCTGAAGCTCTTCA
AGGCGGTGGAAATCCTTGACGATGTCGCCACCATCACCCAGAGCGATGGGAGCTCTCACCTTGCCTTTCTTGACCGACTCCTGGAGGAAGAAGTGGCGGC
AAAGGACAAGCGCCGCGTGGATACCGCCATGAAGATAGCCGGTCTTCCCATGGCCAAGACCATCGAGGAGTACGACTTCACCTTCCACCCCCATCTGGAC
AAGAAAGCGGTGATGGAACTCTTCGATCTGACCTTTCTGGCCAAACACGAAAATGTCATCTTCCTGGGACCGCCGGGAGTGGGTAAGACCCACCTCGCCA
TCGCCCTGGCAATCAAGGCCTGCTATCACGGCTTCAAGGTTTACTTCACCACCATGCACACCCTGATCGCCAAGCTGAAGGAGAGTCAGGCCAAGGGAAA
GGCCTATCTCAACTCAAGCCTGGTGATCGTCGATGAAGTTGGCTACCTGCCGGTCAACAATCAGGAAGCCTACCTCTTCTTCCAGTTCATCTCCTACCGC
TACGAGAAAAGCTCGACCATCATCACCTCCAACAAGAGCTTCTCCGATTGGCAGGAACTCTTCGGAGACCAGGTCATTGCTTCGGCCATCCTGGACCGAC
TGCTTCACCACAGCAAGGTGGTCAACATCAAAGGGCACAGCTACCGGTTACAGGGACATGCCTTCGCCAAGCAACTCTCTCAGAAAGGAGGTGAATCCAT
TTCAACAACACCTGATTGAGGCGCTTCTCCAACCAGCAGGGTGGTACACTTTTCAATTCCCGAAACTGGTACACTTTTACGTCCCCATTGACA
TTCCACGGAGGTAGTTATGGACATAGCCGCACTCAAACGCTCCGGCCACAGTATCCGATGGATCGCACGCAAGCTGGGCATTCATCGCAAGACTGTGAAG
AAGTATCTCGAAAGCAACTCTTTCCCCGCATATCACCGGAAAGACACCAAACCATCCATCCTTGAACCGTATCACCAGATCATCCGGGATTTTCTCGATG
AGGATGAATATCAGGCCACCTGGATACTCACCCGCATCAAAAGGATGGGGTATTCCGGCAGTTACGATACCCTCAAGGTATTCGTCCGCTCCATCAAGGA
ACAGAAGCGTCGCATTGCTTATCAGCGCTTCGAAACGGAGCCCGGTCTCCAGGCCCAGGTTGACTGGGGAGATTTCAAGGTGGTCGATTCCACGGGAAAG
ACCACACCCCTTTACGCCTTCGTAATGGTCCTGGGATTTTCCCGGGCCATGTACGTCGAATTCGTTGAACGATGCACCCTGGAGGCGTTCATGGACTGTC
ACCTCCGGGCATTTCGCCACCTCAAGGGCGTTCCCGCCGAAATCCTCTACGACAACATGAAGCAGGTCGTCGTGGGCCGGGAGAATGGCCGGCCGGTGTT
CAACACCGAATTCCTCCATTTTGCCAGGCACTACGGTTTTACGCCCCGCCTCTGCCCTCCCTACAGCCCCTGGGTAAAGGGAAAGGTCGAACGCCCCATG
CACTATGTGCGGGAAAGCTTCTGGCGGGGGTATGGATTCCATTCTCTGGAAGAGACCAATCAGGATGTTGCTGCCTGGATCGCGGAGACCGCCCACCAGC
GTATCCACGGTACCCACCGTCAGCAGGTTCAGGCTCGCTGGGAGCAGGAACAGCCCTGCCTGGGAGCGCTACCGCCCTCCGAGTACGACACATCCCTCAA
GTATTTTCGCAAGGTCTATAAGGACTGCCTGCTCTCCTTTGGCGGCAACCGCTACTACGTTCCCTACCAGGCCGCGGGGAGAAAGGTCCTTTTGAAGGTC
AAGGCCAGCGTCATTCGGATCTATCACGATGACGAACTGCTGGCCCTGTACCAGATTCCGGAGATCAACGGGCAGACCATCGGCATTCCTGACAAACCGC
CACCCCGGACTCCCCGGCAGACCCCGCGCTATGGTCAGGACCGGGGGATGGCCACCCGTGGTCTCACGACCGGAACGCTCTACCCCGAAGTCTACCGTCG
CCCTCTGGAAGAGTATGACCGCTACGCCGCCGGAGGTGCCCCATGGAACAGCTGACCTATGAGCGCCTCCAGGACAACCTGAAACGGCTGAAGCTCTTCA
AGGCGGTGGAAATCCTTGACGATGTCGCCACCATCACCCAGAGCGATGGGAGCTCTCACCTTGCCTTTCTTGACCGACTCCTGGAGGAAGAAGTGGCGGC
AAAGGACAAGCGCCGCGTGGATACCGCCATGAAGATAGCCGGTCTTCCCATGGCCAAGACCATCGAGGAGTACGACTTCACCTTCCACCCCCATCTGGAC
AAGAAAGCGGTGATGGAACTCTTCGATCTGACCTTTCTGGCCAAACACGAAAATGTCATCTTCCTGGGACCGCCGGGAGTGGGTAAGACCCACCTCGCCA
TCGCCCTGGCAATCAAGGCCTGCTATCACGGCTTCAAGGTTTACTTCACCACCATGCACACCCTGATCGCCAAGCTGAAGGAGAGTCAGGCCAAGGGAAA
GGCCTATCTCAACTCAAGCCTGGTGATCGTCGATGAAGTTGGCTACCTGCCGGTCAACAATCAGGAAGCCTACCTCTTCTTCCAGTTCATCTCCTACCGC
TACGAGAAAAGCTCGACCATCATCACCTCCAACAAGAGCTTCTCCGATTGGCAGGAACTCTTCGGAGACCAGGTCATTGCTTCGGCCATCCTGGACCGAC
TGCTTCACCACAGCAAGGTGGTCAACATCAAAGGGCACAGCTACCGGTTACAGGGACATGCCTTCGCCAAGCAACTCTCTCAGAAAGGAGGTGAATCCAT
TTCAACAACACCTGATTGAGGCGCTTCTCCAACCAGCAGGGTGGTACACTTTTCAATTCCCGAAACTGGTACACTTTTACGTCCCCATTGACA
Protein section
ORF number : 2
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1239 bp | 412 aa | 117 | 1355 | + | No |
Chemistry : DDE
ORF sequence :
MDIAALKRSGHSIRWIARKLGIHRKTVKKYLESNSFPAYHRKDTKPSILEPYHQIIRDFLDEDEYQATWILTRIKRMGYSGSYDTLKVFVRSIKEQKRRI
AYQRFETEPGLQAQVDWGDFKVVDSTGKTTPLYAFVMVLGFSRAMYVEFVERCTLEAFMDCHLRAFRHLKGVPAEILYDNMKQVVVGRENGRPVFNTEFL
HFARHYGFTPRLCPPYSPWVKGKVERPMHYVRESFWRGYGFHSLEETNQDVAAWIAETAHQRIHGTHRQQVQARWEQEQPCLGALPPSEYDTSLKYFRKV
YKDCLLSFGGNRYYVPYQAAGRKVLLKVKASVIRIYHDDELLALYQIPEINGQTIGIPDKPPPRTPRQTPRYGQDRGMATRGLTTGTLYPEVYRRPLEEY
DRYAAGGAPWNS
AYQRFETEPGLQAQVDWGDFKVVDSTGKTTPLYAFVMVLGFSRAMYVEFVERCTLEAFMDCHLRAFRHLKGVPAEILYDNMKQVVVGRENGRPVFNTEFL
HFARHYGFTPRLCPPYSPWVKGKVERPMHYVRESFWRGYGFHSLEETNQDVAAWIAETAHQRIHGTHRQQVQARWEQEQPCLGALPPSEYDTSLKYFRKV
YKDCLLSFGGNRYYVPYQAAGRKVLLKVKASVIRIYHDDELLALYQIPEINGQTIGIPDKPPPRTPRQTPRYGQDRGMATRGLTTGTLYPEVYRRPLEEY
DRYAAGGAPWNS
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 777 bp | 258 aa | 1343 | 2119 | + | No |
AG : IS21 helper
ORF sequence :
MEQLTYERLQDNLKRLKLFKAVEILDDVATITQSDGSSHLAFLDRLLEEEVAAKDKRRVDTAMKIAGLPMAKTIEEYDFTFHPHLDKKAVMELFDLTFLA
KHENVIFLGPPGVGKTHLAIALAIKACYHGFKVYFTTMHTLIAKLKESQAKGKAYLNSSLVIVDEVGYLPVNNQEAYLFFQFISYRYEKSSTIITSNKSF
SDWQELFGDQVIASAILDRLLHHSKVVNIKGHSYRLQGHAFAKQLSQKGGESISTTPD
KHENVIFLGPPGVGKTHLAIALAIKACYHGFKVYFTTMHTLIAKLKESQAKGKAYLNSSLVIVDEVGYLPVNNQEAYLFFQFISYRYEKSSTIITSNKSF
SDWQELFGDQVIASAILDRLLHHSKVVNIKGHSYRLQGHAFAKQLSQKGGESISTTPD
Blast result :
Comments
ISPepr5 is 56%(ORF1) and 65%(ORF2) aa similar to ISMac3.
L1: GAAAAGTGTACCACCCTG
R1: AAAAGTGTACCA
R2: GAAAAGTGTACCACCCTG
L1: GAAAAGTGTACCACCCTG
R1: AAAAGTGTACCA
R2: GAAAAGTGTACCACCCTG
References
1] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina del Rio,T., Hammon,N., Israni,S., Dalin,E., Tice,H., Pitluck,S., Saunders,E., Brettin,T., Bruce,D., Han,C., Tapia,R., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Kim,E., Lovley,D. and Richardson,P. (2006) Direct Submission GenBank.