ISBsp2

  • Family IS3
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NZ_ABCF01000006 ND Bacillus sp.
Bacillus sp. SG-1
DNA section
IS Length : 1459 bp

Ends


IR Length : 29/40

IRL : TGTACCCTATAGAGTAGACACTTAAAAAAGTCTACCTATAGGGTATTTTT
IRR : TGTACCCTTTGTAAAGGACATTTTAAAAAAGCCTAAGCTACTTTTAAAAG

Insertion site


Left flankDirect repeatRight flankDR Length
CTGAATAAAACTTTAGAACG0

DNA sequence

TGTACCCTATAGAGTAGACACTTAAAAAAGTCTACCTATAGGGTATTTTTTTGTATAATGACAAATAGACAACTAGTAGAAATCGGGGAAAACACCATGA
GTAAAAAAACATTCACAGAGAAAGAAATCAAACAATTATCCATTAATCCTTTTGTGAAAGCTGTAAGTGCTAAGGGAATTACCTACACAGATGAATTTAA
ACGCCAATTTATCGCTGAGAAAGAGAAAGGCAAATTCTCTAGACAGATCTTTGAAGAGGCTGGATTTGATACAGATATAATTGGTATGGCACGTATTCAT
GCCGCTAGTAAGAGATGGAGTGCTGCTTATAAAAGAAACGGAGTTTTAGGGCTCAATGACACAAGGAAAGGCAATTCCGGGAGACCTAGAGAACGGGAGT
TATCCCTTGAGGATAAGAATGCTCGACTAGAAGCTCAAATTAACCTCCTTAAGGCGGAAAATGAACTTCTAAAAAAGATTCGATTCGCGGAAAGGGGGCT
AAAGAAGTAGTCCTCTCCCCAAGTCAAAAGTTCATTCTCATCCGTTCCGTAATAGAGAAATATAAGCTCAAAAAAATGGTGAGATTCCTTTGTGGGGTCG
CAGGAGTTTCCAGAAGTGCTTATTATAAATACTACTCTCCCGAGTCCCAAGAGCGTAGAAAACAACGGGAAGCAAGGGATGAGGAACTGAAAGAAATCAT
CTTAAAAGCATTCCGTCATAAGAATCGCAAGAAAGGGGCGCGTCAAATCAAAATGACTTTGGCGGGTCAATTTAAGATTGTCTTTAACTTGAAAAGAATT
CGAAGAATCATGAAGAAGTATAACATCGTTTGTCCCTTCCGGAGGGCGAATCCATATAGGCGTCTAATGAAGGCCACCCAGGAACATTCAGTGGTCCCTA
ACCTACTGGAGCGCCAATTCAATCAGGGTAAACCCGGGAAAGTGCTATTAACAGACATCACCTACTTGAGCTATGGAAAAAGTTCAAGAGCTTATTTATC
TATGATATTGGATGGTTCCAGTGGTGAAGCTCTTGCTTACCATGTGTCTGACAGAATGACTATGGAATTAGCCACAGATACCATCAAAAAACTAAAGAAA
AACAAAAGGTTTAAAAAACATAAAGACTCACTCATCCATTCAGATCAAGGGGTTCACTATACCCATCCCCAATTTCAGAAAATGGTTAAAAAACTTGGAA
TCCGCCAATCAATGTCCCGAAGGGGAAACTGTTGGGACAACGCTCCCATTGAATCGTTCTTTGGCCACCTGAAAGATGAAACCTCTATTAAAACCTGTCT
GACATTAGAGGATGTACAAAAGGAAATCAAGCAATACATGAATTACTATAACCACCATAGATACCAATGGAATCGAAAAAAGATGACCCCTGTACAATAC
AGAGATCATCTTTTAAAAGTAGCTTAGGCTTTTTTAAAATGTCCTTTACAAAGGGTACA
Recoding section
  • Recoding by frameshift
  • Frame
  • Type
  • Experimentally demonstrated

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
453 bp150 aa58510+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MTNRQLVEIGENTMSKKTFTEKEIKQLSINPFVKAVSAKGITYTDEFKRQFIAEKEKGKFSRQIFEEAGFDTDIIGMARIHAASKRWSAAYKRNGVLGLN
DTRKGNSGRPRERELSLEDKNARLEAQINLLKAENELLKKIRFAERGLKK

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
963 bp320 aa4651427+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

TSKKDSIRGKGAKEVVLSPSQKFILIRSVIEKYKLKKMVRFLCGVAGVSRSAYYKYYSPESQERRKQREARDEELKEIILKAFRHKNRKKGARQIKMTLA
GQFKIVFNLKRIRRIMKKYNIVCPFRRANPYRRLMKATQEHSVVPNLLERQFNQGKPGKVLLTDITYLSYGKSSRAYLSMILDGSSGEALAYHVSDRMTM
ELATDTIKKLKKNKRFKKHKDSLIHSDQGVHYTHPQFQKMVKKLGIRQSMSRRGNCWDNAPIESFFGHLKDETSIKTCLTLEDVQKEIKQYMNYYNHHRY
QWNRKKMTPVQYRDHLLKVA

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1370 bp456 aa581427+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MTNRQLVEIGENTMSKKTFTEKEIKQLSINPFVKAVSAKGITYTDEFKRQFIAEKEKGKFSRQIFEEAGFDTDIIGMARIHAASKRWSAAYKRNGVLGLN
DTRKGNSGRPRERELSLEDKNARLEAQINLLKAENELLKKDSIRGKGAKEVVLSPSQKFILIRSVIEKYKLKKMVRFLCGVAGVSRSAYYKYYSPESQER
RKQREARDEELKEIILKAFRHKNRKKGARQIKMTLAGQFKIVFNLKRIRRIMKKYNIVCPFRRANPYRRLMKATQEHSVVPNLLERQFNQGKPGKVLLTD
ITYLSYGKSSRAYLSMILDGSSGEALAYHVSDRMTMELATDTIKKLKKNKRFKKHKDSLIHSDQGVHYTHPQFQKMVKKLGIRQSMSRRGNCWDNAPIES
FFGHLKDETSIKTCLTLEDVQKEIKQYMNYYNHHRYQWNRKKMTPVQYRDHLLKVA

 

Blast result :
Comments
Genome in progress.
ISBsp2 is 78 % (ORFA) and 76% (ORFB) aa similar to ISCpe6. The third ORF is a putative ORFAB transposase reconstructed in sillico by -1 frameshift.
References
1] Tebo,B., Ferriera,S., Johnson,J., Kravitz,S., Beeson,K., Sutton,G., Rogers,Y.-H., Friedman,R., Frazier,M. and Venter,J.C.(2007) Direct submission GenBank.