ISBmu6

  • Family IS3
  • Group IS150
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_010087 ND Burkholderia multivorans
Burkholderia multivorans ATCC 17616 chromosome 3
DNA section
IS Length : 1145 bp

Ends


IR Length : 19/23

IRL : TGAACTGCACCCCAAAAGTTGGATACCGATCCAACCTTTGGGGTGTTTTT
IRR : TGAACTGACCCCGCAAAGTTGGACGGTTAAACACTAGGCGCCCAAGGGCT

Insertion site


Left flankDirect repeatRight flankDR Length
AAAAGCGCGGTATTTAAAGGGCGCTTTTTTCGTTC0

DNA sequence

TGAACTGCACCCCAAAAGTTGGATACCGATCCAACCTTTGGGGTGTTTTTCATGACGAAGTACGACGAGCAATTCAAGCAACGGGTGGTCAGCGCATATT
TGCGTGGCGATAGTGGTTTTGACGCACTGGCAGCCCGTCACGGCATCCATTCCGGAATGTTGCAGCGTTGGGTTGCCGCGTATCGCGCGCATGGCCGTGC
GGGACTGCAGAGCAAATACAGTAGCTACGATGCACGGTTCAAGCTGCGGGTGTTGCAGCACATGCAGCGCGATGAGCTGTCCCTGCGTGAAGTCTCGGCG
CTGTATGACATTCGTGATGCGGGAAGTGTGGCCCGCTGGGCGCGTCTGTATGATGAAGGCGGTATAAACGCTTTGACGCCTCGGCCACGAGGGCGCCCCC
GAAAGATGGTCACATCACTTCCACCCAAGCCCACCGAAGCCGGCACACCGGATGAACGCTCGCGCGAGGAATTGCTCAAGGAAAACGAGTATCTGCGTGC
GGAGGTGGCATACCTAAAAAAGCTCGATGCGCTGCTGGCCAAGAAGCAGGCAGCGCAAAAGAAAAAACGCAAATAGTGCGCGAGCTCAGACAGCATCATC
CGGTGGCAGCGCTTCTGAAGGTGGCCGGTTTGGCGCGCAGCACGTTCTATTACCAGTTGGGGACGCTGAATGCCGACGATCGCCACGCTGATCTGAAGGC
AAAGATCCGGACGGTGTTCGAACATCACAAGGGACGCTACGGCTATCGACGCGTGACGGCTGCCATCCGGCAAGCCGGGTATCTCGTCAACCACAAGGCC
GTGCAGCGGCTGATGCAGCAATTGCAACTGAAGTCCCTGGTGCGCCCGAAGAAATATCGCTCTTGGCGAGGTGAGATCGGGCAGGCCGCACCCAATCTGC
TGCAACGTCAGTTCAGTGCCACGCAGGCCAACCAGAAGTGGGTAACGGACGTGACGGAGTTCAACGTCGGCGGGCAGAAGTTGTATCTATCGCCGGTGAT
GGATCTATACAACGGCGAGATCGTGGCTTACCAGATGGATCAACGTCCAAGCTTCGAACTGGTCAGCGGCATGCTGAAGAAGGCGCTGGCCAAGCTGCGG
CGCAAGGACAAACCGTTATTGCATTCGGATCAAGGCTGGCAATACCGGATGCCGGCGTATCGCCGGGAACTGAAGAAGCGCAAGCTGACCCAGAGCATGT
CGCGCAAGGGCAATTGCCTGGACAACGCCGCGATGGAAAGCTTCTTCGGCACGCTCAAGTCTGAGTGCTACAACGGGCATTGTTTCACTTGTGTAGAGCA
ATTGCGCGACACGCTGGACCACTACATCCACTACTACAACCACGAACGCATCAAGCTCAAATTGAAAGGGCTGAGTCCCGTGCAATACAGAACTCAGCCC
TTGGGCGCCTAGTGTTTAACCGTCCAACTTTGCGGGGTCAGTTCA
Recoding section
  • Recoding by frameshift
  • Frame
  • Type
  • Experimentally demonstrated

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
525 bp174 aa52576+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MTKYDEQFKQRVVSAYLRGDSGFDALAARHGIHSGMLQRWVAAYRAHGRAGLQSKYSSYDARFKLRVLQHMQRDELSLREVSALYDIRDAGSVARWARLY
DEGGINALTPRPRGRPRKMVTSLPPKPTEAGTPDERSREELLKENEYLRAEVAYLKKLDALLAKKQAAQKKKRK

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
957 bp318 aa4561412+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

TLARGIAQGKRVSACGGGIPKKARCAAGQEAGSAKEKTQIVRELRQHHPVAALLKVAGLARSTFYYQLGTLNADDRHADLKAKIRTVFEHHKGRYGYRRV
TAAIRQAGYLVNHKAVQRLMQQLQLKSLVRPKKYRSWRGEIGQAAPNLLQRQFSATQANQKWVTDVTEFNVGGQKLYLSPVMDLYNGEIVAYQMDQRPSF
ELVSGMLKKALAKLRRKDKPLLHSDQGWQYRMPAYRRELKKRKLTQSMSRKGNCLDNAAMESFFGTLKSECYNGHCFTCVEQLRDTLDHYIHYYNHERIK
LKLKGLSPVQYRTQPLGA

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1361 bp453 aa521412+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MTKYDEQFKQRVVSAYLRGDSGFDALAARHGIHSGMLQRWVAAYRAHGRAGLQSKYSSYDARFKLRVLQHMQRDELSLREVSALYDIRDAGSVARWARLY
DEGGINALTPRPRGRPRKMVTSLPPKPTEAGTPDERSREELLKENEYLRAEVAYLKKARCAAGQEAGSAKEKTQIVRELRQHHPVAALLKVAGLARSTFY
YQLGTLNADDRHADLKAKIRTVFEHHKGRYGYRRVTAAIRQAGYLVNHKAVQRLMQQLQLKSLVRPKKYRSWRGEIGQAAPNLLQRQFSATQANQKWVTD
VTEFNVGGQKLYLSPVMDLYNGEIVAYQMDQRPSFELVSGMLKKALAKLRRKDKPLLHSDQGWQYRMPAYRRELKKRKLTQSMSRKGNCLDNAAMESFFG
TLKSECYNGHCFTCVEQLRDTLDHYIHYYNHERIKLKLKGLSPVQYRTQPLGA

 

Blast result :
Comments
ISBmu6 is 97% aa similar to ISBcen16.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] NCBI Genome Project (2007) Direct Submission GenBank.
2] ISfinder annotation (2008).