ISSpn4
- Family IS3
- Group IS150
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NZ_ABAC01000016 | ND | Streptococcus pneumoniae | Streptococcus pneumoniae SP11-BS70 |
DNA section
IS Length : 1438 bp
Ends
IR Length : 22/25
IRL : TGAACTGCACCCCAAAAGTTAGACAGAAAAAATCTACTTTTTGGGGTGTT
IRR : TGTACTGACCCCCAAAAGTTAGACAATTAATTTATCCGAAGGATTTAGTT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| AAGAAAAAAGCAGTACA | AGA | TTGAACTGCTTTTACGGAT | 3 |
DNA sequence
TGAACTGCACCCCAAAAGTTAGACAGAAAAAATCTACTTTTTGGGGTGTTTTATTATGAAATTGAGTTATGAGGATAAAGTTCAGATCTATGAACTAAGA
AAGCAAGGATATAGCTTAGAGAAGATTTCAAATAAATTTGAGATAAACAATTCTAATCTTAGGTACATGATTAAATTGATTGATCGTTACGGAATAGAGT
TCATCAAAAAAGGGAAAAATCGATACTATTCTCCAGAACTAAAACAAGAAATGATTGATAAAGTTTTACATGAAAACTGGTCTCAAGATAGAGTTTCTCT
CAAATATGCTCTCCCAAATCGTGGTATGCTTCCAAATTGGCTGGCGCAATACAAGAAAAACGGGTATACTATTGTTGAGAAAACAAGAGAGAGAGTACCT
AAAATGGGACGTAAACGTAAGAAAACTTGGGAAGAAATGACAGAACTAGAGCGACTTCAAGAGGAGAATGAACGCTTACGTACCGAGGTGGCTTACTTAA
AAAAGTTAAAAGAGCTGGAAGACAGGGACGAAGCCCTAGAGCGAGAAAGGCAGAGATAGTTAGAGAAATGGCTTCAGGAGGATTTCAATTAGATTTACTT
CTTGAAACAGCACGTTTAGCTCGCTCGACTTACTACTATCAGTTGAAGGGACTAGGTGGGCTTGACAAAGATAAAGATCTTAAAGCTGAAATTCAAGCTA
TTTTTACTGAGCACAAAGGAAATTATGGCTATCGTAGAGTTACTCTTGAACTAAGAAATCGTGGTTTTGTGGTGAATCATAAGAAAGTTCAACGTCTGAT
GAAAGTTCTTGGTTTAACAGCTCGAATTCGTCGGAAACGAAAGTATTCCTCCTACCAAGGAGAGATTGGCAAGAAAGCAGAGAATCTCATTCAAGGCCAG
TTCGAAGCATCGAGACCAATGGAAAAGTGCTATACGGATGTGACAGAGTTTGCCATTCCAGCAAGCAGTCAAAAGCTCTATTTATCGCCAGTTTTAGATG
GCTTTAACAGCGAAATTATCGCCTATAATCTTTCAACTTCACCAAACTTAGAACAAGGGAAAGCTATGTTAAACCAGGCCTTTACAGAGAAACATTATGA
GAATACCATTCTCCATAGCGATCAAGGCTGGCAATACCAACACGATTCTTATCATCGGTTCCTAGAGAGTAAGGGAATTCAAGCATCCATGTCACGCAAG
GGAAACAGCCCAGACAATGGCATGATGGAGTCCTTCTTTGGCATTTTGAAATCCGAAATGTTTTATGGTTATGAGAAGACATTTCAGTCGCTTAATCAAT
TGGAACAAGCTATTGTAGACTATATTGATTATTACAACAACAAACGAATTAAGATAAAACTAAAAGGACTTAGTCCTGTGCAATACAGAACTAAATCCTT
CGGATAAATTAATTGTCTAACTTTTGGGGGTCAGTACA
AAGCAAGGATATAGCTTAGAGAAGATTTCAAATAAATTTGAGATAAACAATTCTAATCTTAGGTACATGATTAAATTGATTGATCGTTACGGAATAGAGT
TCATCAAAAAAGGGAAAAATCGATACTATTCTCCAGAACTAAAACAAGAAATGATTGATAAAGTTTTACATGAAAACTGGTCTCAAGATAGAGTTTCTCT
CAAATATGCTCTCCCAAATCGTGGTATGCTTCCAAATTGGCTGGCGCAATACAAGAAAAACGGGTATACTATTGTTGAGAAAACAAGAGAGAGAGTACCT
AAAATGGGACGTAAACGTAAGAAAACTTGGGAAGAAATGACAGAACTAGAGCGACTTCAAGAGGAGAATGAACGCTTACGTACCGAGGTGGCTTACTTAA
AAAAGTTAAAAGAGCTGGAAGACAGGGACGAAGCCCTAGAGCGAGAAAGGCAGAGATAGTTAGAGAAATGGCTTCAGGAGGATTTCAATTAGATTTACTT
CTTGAAACAGCACGTTTAGCTCGCTCGACTTACTACTATCAGTTGAAGGGACTAGGTGGGCTTGACAAAGATAAAGATCTTAAAGCTGAAATTCAAGCTA
TTTTTACTGAGCACAAAGGAAATTATGGCTATCGTAGAGTTACTCTTGAACTAAGAAATCGTGGTTTTGTGGTGAATCATAAGAAAGTTCAACGTCTGAT
GAAAGTTCTTGGTTTAACAGCTCGAATTCGTCGGAAACGAAAGTATTCCTCCTACCAAGGAGAGATTGGCAAGAAAGCAGAGAATCTCATTCAAGGCCAG
TTCGAAGCATCGAGACCAATGGAAAAGTGCTATACGGATGTGACAGAGTTTGCCATTCCAGCAAGCAGTCAAAAGCTCTATTTATCGCCAGTTTTAGATG
GCTTTAACAGCGAAATTATCGCCTATAATCTTTCAACTTCACCAAACTTAGAACAAGGGAAAGCTATGTTAAACCAGGCCTTTACAGAGAAACATTATGA
GAATACCATTCTCCATAGCGATCAAGGCTGGCAATACCAACACGATTCTTATCATCGGTTCCTAGAGAGTAAGGGAATTCAAGCATCCATGTCACGCAAG
GGAAACAGCCCAGACAATGGCATGATGGAGTCCTTCTTTGGCATTTTGAAATCCGAAATGTTTTATGGTTATGAGAAGACATTTCAGTCGCTTAATCAAT
TGGAACAAGCTATTGTAGACTATATTGATTATTACAACAACAAACGAATTAAGATAAAACTAAAAGGACTTAGTCCTGTGCAATACAGAACTAAATCCTT
CGGATAAATTAATTGTCTAACTTTTGGGGGTCAGTACA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 504 bp | 167 aa | 56 | 559 | + | No |
Description : First part of the transposase
ORF sequence :
MKLSYEDKVQIYELRKQGYSLEKISNKFEINNSNLRYMIKLIDRYGIEFIKKGKNRYYSPELKQEMIDKVLHENWSQDRVSLKYALPNRGMLPNWLAQYK
KNGYTIVEKTRERVPKMGRKRKKTWEEMTELERLQEENERLRTEVAYLKKLKELEDRDEALERERQR
KNGYTIVEKTRERVPKMGRKRKKTWEEMTELERLQEENERLRTEVAYLKKLKELEDRDEALERERQR
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 936 bp | 311 aa | 472 | 1407 | + | No |
Description : Second part of the transposase
ORF sequence :
TLTYRGGLLKKVKRAGRQGRSPRARKAEIVREMASGGFQLDLLLETARLARSTYYYQLKGLGGLDKDKDLKAEIQAIFTEHKGNYGYRRVTLELRNRGFV
VNHKKVQRLMKVLGLTARIRRKRKYSSYQGEIGKKAENLIQGQFEASRPMEKCYTDVTEFAIPASSQKLYLSPVLDGFNSEIIAYNLSTSPNLEQGKAML
NQAFTEKHYENTILHSDQGWQYQHDSYHRFLESKGIQASMSRKGNSPDNGMMESFFGILKSEMFYGYEKTFQSLNQLEQAIVDYIDYYNNKRIKIKLKGL
SPVQYRTKSFG
VNHKKVQRLMKVLGLTARIRRKRKYSSYQGEIGKKAENLIQGQFEASRPMEKCYTDVTEFAIPASSQKLYLSPVLDGFNSEIIAYNLSTSPNLEQGKAML
NQAFTEKHYENTILHSDQGWQYQHDSYHRFLESKGIQASMSRKGNSPDNGMMESFFGILKSEMFYGYEKTFQSLNQLEQAIVDYIDYYNNKRIKIKLKGL
SPVQYRTKSFG
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1352 bp | 450 aa | 56 | 1407 | + | Yes |
Chemistry : DDE
ORF sequence :
MKLSYEDKVQIYELRKQGYSLEKISNKFEINNSNLRYMIKLIDRYGIEFIKKGKNRYYSPELKQEMIDKVLHENWSQDRVSLKYALPNRGMLPNWLAQYK
KNGYTIVEKTRERVPKMGRKRKKTWEEMTELERLQEENERLRTEVAYLKKVKRAGRQGRSPRARKAEIVREMASGGFQLDLLLETARLARSTYYYQLKGL
GGLDKDKDLKAEIQAIFTEHKGNYGYRRVTLELRNRGFVVNHKKVQRLMKVLGLTARIRRKRKYSSYQGEIGKKAENLIQGQFEASRPMEKCYTDVTEFA
IPASSQKLYLSPVLDGFNSEIIAYNLSTSPNLEQGKAMLNQAFTEKHYENTILHSDQGWQYQHDSYHRFLESKGIQASMSRKGNSPDNGMMESFFGILKS
EMFYGYEKTFQSLNQLEQAIVDYIDYYNNKRIKIKLKGLSPVQYRTKSFG
KNGYTIVEKTRERVPKMGRKRKKTWEEMTELERLQEENERLRTEVAYLKKVKRAGRQGRSPRARKAEIVREMASGGFQLDLLLETARLARSTYYYQLKGL
GGLDKDKDLKAEIQAIFTEHKGNYGYRRVTLELRNRGFVVNHKKVQRLMKVLGLTARIRRKRKYSSYQGEIGKKAENLIQGQFEASRPMEKCYTDVTEFA
IPASSQKLYLSPVLDGFNSEIIAYNLSTSPNLEQGKAMLNQAFTEKHYENTILHSDQGWQYQHDSYHRFLESKGIQASMSRKGNSPDNGMMESFFGILKS
EMFYGYEKTFQSLNQLEQAIVDYIDYYNNKRIKIKLKGLSPVQYRTKSFG
Blast result :
Comments
Genome in progress.
ISSpn4 is 87% aa similar to ISSmu1.
The third ORF is putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
ISSpn4 is 87% aa similar to ISSmu1.
The third ORF is putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Ehrlich,G.D. (2007) Direct submission GenBank.