ISCro3
- Family IS4
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Citrobacter rodentium | Citrobacter rodentium ICC168 |
DNA section
IS Length : 1430 bp
Ends
IR Length : 18
IRL : TAATGCCGATCAGTTAAGGATCGGTTGACCGATCCTTAATCTGCGGCACT
IRR : TAATGCCGTTCACTTAAGCGGTGCGGCATTGCGTTTCACCGGATAACGGG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|
DNA sequence
TAATGCCGATCAGTTAAGGATCGGTTGACCGATCCTTAATCTGCGGCACTATAACGGCTTCCACAACAGGGAGCCGTTTTCTTATGCCACTTCTCAATGA
CCTGCTCGATTTCAGCGACCATCCGCTGATGCCGCCCCCTTCTGCACAACTGTTTGCTCAGCATCTTCCCGTCGAATGGATACAACACTGCCTGACTCTT
TCTGCACATGCGACCGTTCGCCGCCGTCGTTTACCTGGCGACATGGTTATCTGGATGGTCGTGGCTATGGCCTTCTTCCGTAATGAGTCGATTACCGATG
TGGTTCGTCGTCTGAACCTGAGCGCGGATGGTGAAGCGGGGATGAACCTGCTGGCCCGCAGCGCTGTCACCCAGGCGCGTCAGCGCGTGGGTGCTGCACC
GGTGGAATGGCTTTTCCGCCAGACCGCGCAGACATGGGGAACTGAGCGTTACCAGAAAGATGACTGGCATGGCCTGCAACTTTTTGCCATCGATGGCGCA
CAGTTCAGGACACCTGATGAACCTGAGCTGCGTGAACATTATGGCTCTGCCAACACATCCACTGAGCGGCAAAGCGCATACCCGGTAATGCGTCTGGTGG
CTTTAATGAACCTGGGTAGTCACATTCTGCTGGATGCCGCGACCGCGCCTTACCGACGGAGCGAAACCCTGCTGGCCCACTCAATGCTCGTCACCATTCC
GGATAACTCCATTACGCTGTTTGACAAGCTGTTCTACAGCGCAGACCTGTTGCTGTCGCTGAACCGGCAGGGTTGTAACCGCCACTGGCTGCTGCCCGCC
TGGAAGAATATCGCAGCAGAAACGGAAGAAAGTTACGGTCCCGGAGACCGGCTTCTGAAACTGAAAGTGTCACCACAGGCAAGGAAAAAGAATCCTTCGC
TGCCGGAATACTGGTATGCGCGGGCGGTAACTTATGAACTGAACGGAGTGGAAAAAACGGTCCTGACGTCACTTCAGGCAGACCGTTATAAGGCCAGAGA
GGTGGCAGAGCTTTATCACTCAAGATGGGAAATCGAAGTCGGGTTCAGAAACCTGAAAAGCAGCCTGCTGAATAACGCTCTGGTACTGAGAAGCCGGAAG
GTTGAGTTGCTGGAACAGGAGGTGTGGGGCATGTTGCTGGCTTATAATCTGATACGGCGTGAGGCAACAAAAGCAACGGAGAAACACAAAAAAGCGGCGT
CGGAAATCAGCTTTAAGTTCGCGTTCCAGTTTATCGCCACAGAAATGATAGTGCTGGGAAATACGGTGTCACCGGGGACCATCCCGAAACGGCTGGAGCA
TCTGCGGGGGGCTCTGGAAGCGGTGTTCATAACAAAACGCCCCCGACCATCAAGGCCGAGGGCGGTTAAGATATCAAAAACCCGTTATCCGGTGAAACGC
AATGCCGCACCGCTTAAGTGAACGGCATTA
CCTGCTCGATTTCAGCGACCATCCGCTGATGCCGCCCCCTTCTGCACAACTGTTTGCTCAGCATCTTCCCGTCGAATGGATACAACACTGCCTGACTCTT
TCTGCACATGCGACCGTTCGCCGCCGTCGTTTACCTGGCGACATGGTTATCTGGATGGTCGTGGCTATGGCCTTCTTCCGTAATGAGTCGATTACCGATG
TGGTTCGTCGTCTGAACCTGAGCGCGGATGGTGAAGCGGGGATGAACCTGCTGGCCCGCAGCGCTGTCACCCAGGCGCGTCAGCGCGTGGGTGCTGCACC
GGTGGAATGGCTTTTCCGCCAGACCGCGCAGACATGGGGAACTGAGCGTTACCAGAAAGATGACTGGCATGGCCTGCAACTTTTTGCCATCGATGGCGCA
CAGTTCAGGACACCTGATGAACCTGAGCTGCGTGAACATTATGGCTCTGCCAACACATCCACTGAGCGGCAAAGCGCATACCCGGTAATGCGTCTGGTGG
CTTTAATGAACCTGGGTAGTCACATTCTGCTGGATGCCGCGACCGCGCCTTACCGACGGAGCGAAACCCTGCTGGCCCACTCAATGCTCGTCACCATTCC
GGATAACTCCATTACGCTGTTTGACAAGCTGTTCTACAGCGCAGACCTGTTGCTGTCGCTGAACCGGCAGGGTTGTAACCGCCACTGGCTGCTGCCCGCC
TGGAAGAATATCGCAGCAGAAACGGAAGAAAGTTACGGTCCCGGAGACCGGCTTCTGAAACTGAAAGTGTCACCACAGGCAAGGAAAAAGAATCCTTCGC
TGCCGGAATACTGGTATGCGCGGGCGGTAACTTATGAACTGAACGGAGTGGAAAAAACGGTCCTGACGTCACTTCAGGCAGACCGTTATAAGGCCAGAGA
GGTGGCAGAGCTTTATCACTCAAGATGGGAAATCGAAGTCGGGTTCAGAAACCTGAAAAGCAGCCTGCTGAATAACGCTCTGGTACTGAGAAGCCGGAAG
GTTGAGTTGCTGGAACAGGAGGTGTGGGGCATGTTGCTGGCTTATAATCTGATACGGCGTGAGGCAACAAAAGCAACGGAGAAACACAAAAAAGCGGCGT
CGGAAATCAGCTTTAAGTTCGCGTTCCAGTTTATCGCCACAGAAATGATAGTGCTGGGAAATACGGTGTCACCGGGGACCATCCCGAAACGGCTGGAGCA
TCTGCGGGGGGCTCTGGAAGCGGTGTTCATAACAAAACGCCCCCGACCATCAAGGCCGAGGGCGGTTAAGATATCAAAAACCCGTTATCCGGTGAAACGC
AATGCCGCACCGCTTAAGTGAACGGCATTA
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1338 bp | 445 aa | 84 | 1421 | + | No |
Chemistry : DDE
ORF sequence :
MPLLNDLLDFSDHPLMPPPSAQLFAQHLPVEWIQHCLTLSAHATVRRRRLPGDMVIWMVVAMAFFRNESITDVVRRLNLSADGEAGMNLLARSAVTQARQ
RVGAAPVEWLFRQTAQTWGTERYQKDDWHGLQLFAIDGAQFRTPDEPELREHYGSANTSTERQSAYPVMRLVALMNLGSHILLDAATAPYRRSETLLAHS
MLVTIPDNSITLFDKLFYSADLLLSLNRQGCNRHWLLPAWKNIAAETEESYGPGDRLLKLKVSPQARKKNPSLPEYWYARAVTYELNGVEKTVLTSLQAD
RYKAREVAELYHSRWEIEVGFRNLKSSLLNNALVLRSRKVELLEQEVWGMLLAYNLIRREATKATEKHKKAASEISFKFAFQFIATEMIVLGNTVSPGTI
PKRLEHLRGALEAVFITKRPRPSRPRAVKISKTRYPVKRNAAPLK
RVGAAPVEWLFRQTAQTWGTERYQKDDWHGLQLFAIDGAQFRTPDEPELREHYGSANTSTERQSAYPVMRLVALMNLGSHILLDAATAPYRRSETLLAHS
MLVTIPDNSITLFDKLFYSADLLLSLNRQGCNRHWLLPAWKNIAAETEESYGPGDRLLKLKVSPQARKKNPSLPEYWYARAVTYELNGVEKTVLTSLQAD
RYKAREVAELYHSRWEIEVGFRNLKSSLLNNALVLRSRKVELLEQEVWGMLLAYNLIRREATKATEKHKKAASEISFKFAFQFIATEMIVLGNTVSPGTI
PKRLEHLRGALEAVFITKRPRPSRPRAVKISKTRYPVKRNAAPLK
Blast result :
Comments
ISCro3 is 74% aa similar to ISVvu3.
11 intact copies and 2 remnants of ISCro3 in the Citrobacter rodentium genome at genome co-ordinates (312071..313500; 895787..897216; 1188783..1189281 and 1189891..1190354 (remnant); 1228460..1229889; 1861572..1863001; 2014596..2016025; 2397591..2399020; 2669098..2670527; 4247281..4248710; 4317177..4317334 (remnant); 4408192..4409621; 4494090..4495519; 4956020..4957449.
Number of base pairs in direct repeat varies, of the 11 intact copies of ISCro3, 2 have no direct repeats (DR); 2 have 10 bp DR; 6 have 11 bp DR, and 1 has 12 bp DR
11 intact copies and 2 remnants of ISCro3 in the Citrobacter rodentium genome at genome co-ordinates (312071..313500; 895787..897216; 1188783..1189281 and 1189891..1190354 (remnant); 1228460..1229889; 1861572..1863001; 2014596..2016025; 2397591..2399020; 2669098..2670527; 4247281..4248710; 4317177..4317334 (remnant); 4408192..4409621; 4494090..4495519; 4956020..4957449.
Number of base pairs in direct repeat varies, of the 11 intact copies of ISCro3, 2 have no direct repeats (DR); 2 have 10 bp DR; 6 have 11 bp DR, and 1 has 12 bp DR
References
1] Nicola Petty (20007) Direct submission .