ISVisp5
- Family IS3
- Group IS3
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Vibrio splendidus | Vibrio splendidus |
DNA section
IS Length : 1243 bp
Ends
IR Length : 26
IRL : TGATCCGCACCAGCAGTTTTGGACACTGAGTTAAGTGAGTACAATCACTA
IRR : TGATCCGGTCCAGCCAGACTGGACACTTCATTAAGCGACATTTTGTTGTT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| NNNNNNNNNN | TA | NNNNNNNNNN | 2 |
| NNNNNNNNNN | TT | NNNNNNNNNN | 2 |
DNA sequence
TGATCCGCACCAGCAGTTTTGGACACTGAGTTAAGTGAGTACAATCACTAATGAGGTGAACCATGACAAGTAAGAAGAAACGTATTATCCATTCTCCTGA
ATTTAAAGCAGAAACCCTGAAGCTGGCGGAGAAAGTGGGAGTCGCTGCAGCCGCAAGACAGCTCTCATTACATGAATCTCAGATCTACGGTTGGCGAAAA
GCCACAAAGAAGAATTCTAATATTAGCCAACGAGAACAAGAATTGGCCGTTGAAGTCGCTAAGCTAAAGAGGCAGTTGGCAGAGCAAGCAGAAGAGCTAG
AAATCGTAAAAAAGGCCGCCACCTACTTCGCGAAAAATCTAAAGTAAATTGCTATGAATTTATGCTCGAACACCTGCTGAGTTTCAGTATTTCCCGTATG
GCTAAGGTGTTCAGAGTTTCTCGAAGTGGGTTTTATTATTGGGTTGAGAATCGCCACAAGGCGATTCAACGTGAGACAGAGCGCCAAAAGCTTGATATCA
AGGTAAAAGAAGCCTTTGACGTTAGCAAAGAACGAGATGGCTCAAGACGTATTCAGAAAGAACTGGCAGAGAGCGGTGATAACCATAATGTTAAGACCAT
TGCCGCCAGTATGAAACGTCAGGATTTAGTAGCGAAAGCAGCACGCAAGTTTAAGTGTACAACGGACAGTAAGCATAAAATGCCCGTTGCTCCGAACTTG
CTCGCTCAAGATTTTAGCGCAACGGCTCCGAATCAAAAATGGGCAGGAGACATCACCTATGTTGCCACAAGCGAAGGCTGGCTGTACTTAGCTGTCATTA
TTGACCTTTATTCAAGGCAAGTTATTGGTTGGTCTATGGATACGAGAATGACGGCCTCACTGGCCTGTGATGCCTTATCGATGGCTTTATTCCGTCGAGG
GTTCCCTGAGCAGGTTATAACTCATAGCGATCGAGGTAGTCAGTACTGTTCGAAAGACTATCGAGACCTCATAAGTAATTATAATCTAAGGCAAAGTATG
AGTAGGAAAGGAAATTGCTGGGATAATGCTTGTGTTGAGAGCTTCTTTCATTCTATGAAAGTAGAAGCAATCCAATATGAGCCGATCATGACGAGAGAGC
AGATGCGTCAAACGATCTTCGAGTACATTGAGGTTGATTATAATCGAACGAGAAGGCACAGTGATCTTGGGTACCTAAGCCCAGTTAATTTTGAACAACA
AAATGTCGCTTAATGAAGTGTCCAGTCTGGCTGGACCGGATCA
ATTTAAAGCAGAAACCCTGAAGCTGGCGGAGAAAGTGGGAGTCGCTGCAGCCGCAAGACAGCTCTCATTACATGAATCTCAGATCTACGGTTGGCGAAAA
GCCACAAAGAAGAATTCTAATATTAGCCAACGAGAACAAGAATTGGCCGTTGAAGTCGCTAAGCTAAAGAGGCAGTTGGCAGAGCAAGCAGAAGAGCTAG
AAATCGTAAAAAAGGCCGCCACCTACTTCGCGAAAAATCTAAAGTAAATTGCTATGAATTTATGCTCGAACACCTGCTGAGTTTCAGTATTTCCCGTATG
GCTAAGGTGTTCAGAGTTTCTCGAAGTGGGTTTTATTATTGGGTTGAGAATCGCCACAAGGCGATTCAACGTGAGACAGAGCGCCAAAAGCTTGATATCA
AGGTAAAAGAAGCCTTTGACGTTAGCAAAGAACGAGATGGCTCAAGACGTATTCAGAAAGAACTGGCAGAGAGCGGTGATAACCATAATGTTAAGACCAT
TGCCGCCAGTATGAAACGTCAGGATTTAGTAGCGAAAGCAGCACGCAAGTTTAAGTGTACAACGGACAGTAAGCATAAAATGCCCGTTGCTCCGAACTTG
CTCGCTCAAGATTTTAGCGCAACGGCTCCGAATCAAAAATGGGCAGGAGACATCACCTATGTTGCCACAAGCGAAGGCTGGCTGTACTTAGCTGTCATTA
TTGACCTTTATTCAAGGCAAGTTATTGGTTGGTCTATGGATACGAGAATGACGGCCTCACTGGCCTGTGATGCCTTATCGATGGCTTTATTCCGTCGAGG
GTTCCCTGAGCAGGTTATAACTCATAGCGATCGAGGTAGTCAGTACTGTTCGAAAGACTATCGAGACCTCATAAGTAATTATAATCTAAGGCAAAGTATG
AGTAGGAAAGGAAATTGCTGGGATAATGCTTGTGTTGAGAGCTTCTTTCATTCTATGAAAGTAGAAGCAATCCAATATGAGCCGATCATGACGAGAGAGC
AGATGCGTCAAACGATCTTCGAGTACATTGAGGTTGATTATAATCGAACGAGAAGGCACAGTGATCTTGGGTACCTAAGCCCAGTTAATTTTGAACAACA
AAATGTCGCTTAATGAAGTGTCCAGTCTGGCTGGACCGGATCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 285 bp | 94 aa | 63 | 347 | + | No |
Description : First part of the transposase
ORF sequence :
MTSKKKRIIHSPEFKAETLKLAEKVGVAAAARQLSLHESQIYGWRKATKKNSNISQREQELAVEVAKLKRQLAEQAEELEIVKKAATYFAKNLK
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 921 bp | 306 aa | 293 | 1213 | + | No |
Description : Second part of the transposase
ORF sequence :
AKEAVGRASRRARNRKKGRHLLREKSKVNCYEFMLEHLLSFSISRMAKVFRVSRSGFYYWVENRHKAIQRETERQKLDIKVKEAFDVSKERDGSRRIQKE
LAESGDNHNVKTIAASMKRQDLVAKAARKFKCTTDSKHKMPVAPNLLAQDFSATAPNQKWAGDITYVATSEGWLYLAVIIDLYSRQVIGWSMDTRMTASL
ACDALSMALFRRGFPEQVITHSDRGSQYCSKDYRDLISNYNLRQSMSRKGNCWDNACVESFFHSMKVEAIQYEPIMTREQMRQTIFEYIEVDYNRTRRHS
DLGYLSPVNFEQQNVA
LAESGDNHNVKTIAASMKRQDLVAKAARKFKCTTDSKHKMPVAPNLLAQDFSATAPNQKWAGDITYVATSEGWLYLAVIIDLYSRQVIGWSMDTRMTASL
ACDALSMALFRRGFPEQVITHSDRGSQYCSKDYRDLISNYNLRQSMSRKGNCWDNACVESFFHSMKVEAIQYEPIMTREQMRQTIFEYIEVDYNRTRRHS
DLGYLSPVNFEQQNVA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1151 bp | 383 aa | 63 | 1213 | + | Yes |
Chemistry : DDE
ORF sequence :
MTSKKKRIIHSPEFKAETLKLAEKVGVAAAARQLSLHESQIYGWRKATKKNSNISQREQELAVEVAKLKRQLAEQAEELEIVKKGRHLLREKSKVNCYEF
MLEHLLSFSISRMAKVFRVSRSGFYYWVENRHKAIQRETERQKLDIKVKEAFDVSKERDGSRRIQKELAESGDNHNVKTIAASMKRQDLVAKAARKFKCT
TDSKHKMPVAPNLLAQDFSATAPNQKWAGDITYVATSEGWLYLAVIIDLYSRQVIGWSMDTRMTASLACDALSMALFRRGFPEQVITHSDRGSQYCSKDY
RDLISNYNLRQSMSRKGNCWDNACVESFFHSMKVEAIQYEPIMTREQMRQTIFEYIEVDYNRTRRHSDLGYLSPVNFEQQNVA
MLEHLLSFSISRMAKVFRVSRSGFYYWVENRHKAIQRETERQKLDIKVKEAFDVSKERDGSRRIQKELAESGDNHNVKTIAASMKRQDLVAKAARKFKCT
TDSKHKMPVAPNLLAQDFSATAPNQKWAGDITYVATSEGWLYLAVIIDLYSRQVIGWSMDTRMTASLACDALSMALFRRGFPEQVITHSDRGSQYCSKDY
RDLISNYNLRQSMSRKGNCWDNACVESFFHSMKVEAIQYEPIMTREQMRQTIFEYIEVDYNRTRRHSDLGYLSPVNFEQQNVA
Blast result :
Comments
ISVisp5 has 85% nucleotide sequence homology with ISVpa2 of Vibrio parahaemolyticus.
ISVisp5 is 94% (ORFA) and 93% (ORFB) aa similar to ISVpa2. The third ORF is a putative ORFAB transposase predicted in silico by possible -1 frameshift (A AAA AAG at position 308 to 314)
ISVisp5 is 94% (ORFA) and 93% (ORFB) aa similar to ISVpa2. The third ORF is a putative ORFAB transposase predicted in silico by possible -1 frameshift (A AAA AAG at position 308 to 314)
References
1] Mitsuaki Nishibuchi (2007) Direct submission.