ISNieu4
- Family IS5
- Group IS1031
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_008344 | ND | Nitrosomonas eutropha | Nitrosomonas eutropha C91 |
DNA section
IS Length : 842 bp
Ends
IR Length : 22/26
IRL : GAACCTGTTCAAAGTCTAGGGGAGTATGAGAAACTCGGAAGATGAGAAGA
IRR : GAACCTGTTCAAAGTCTTTTGAGTAGAAGGGCCAGGAAGGCCAGGTGAAT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| ACCAGCCTTA | TCAGAAAATA | 0 | |
| ACCTATCATC | TCA | TGACTTTCAG | 3 |
| AATTTCCTTC | TAA | CAAGATTATT | 3 |
| ATAATAAATC | TGA | GGCAGCCAAT | 3 |
| GACTAAACAC | TTA | CACCCAAAGA | 3 |
| GGAATACCTC | TCA | GAACTGAGTG | 3 |
| TGAATCAGCG | TAA | GTGTTTAGTC | 3 |
| ATGAGTTGTC | TAA | GTAATTGTGC | 3 |
| GCAGGTTCTC | TAA | GTGTTTAGTC | 3 |
| GACTAAACAC | TTA | CATTTTCTAT | 3 |
| CTCCATCTTC | TCA | GATAGTGGAG | 3 |
DNA sequence
GAACCTGTTCAAAGTCTAGGGGAGTATGAGAAACTCGGAAGATGAGAAGAAGTTACCCGAGCGACATCAGTCGTGAACAATTCGAGCTTATCAAGCCTTT
GCTGGAGAGCGCGCGCAGGAAGACCAGCCCTCGACGAATAGATCTGTACGATGTTTTTTGTGCGGTGCTGTATTTGCTCAAGAGCGGCTGCCAGTGGCGA
GCCCTGCCTAATGACTTTCCCAAGTGGCGCACTGTCCACTCATACTGGGCCATCTGGAGTGAGCCGCGCGAAGGAGGCAGCCTGCTGGAGCAGGCATTAA
AAAAATCAGGTTGGCGAGGTTCGTACCAGACAGGGGCGCAACGCCTGCACGTCATTCTTGATCGTGGACGCGCAGAGCGTGAAGAACACGGACACGGCGA
GCCAGAAGGGCTATGACGCAGGCAAGAAGGTATCGGGCATCAAGCGTCATATCGCGGTCGATACCCAGGGTTTGCCGCATGCCATTGCGGTGACGACAGC
GGAAGTGACCGATCGCAAAGGTGCGTTGCAGGCTTTCCGGCGTTGTCGTTCAGGCCTGGGCCGGGTGGTGGCGGTGTTGGCGGACAGCGGTTATGTGGGA
GAGCCGTTTGCTCAAGGTGTACGCGATGTGCTGAGCGAGACTGTCGCGGTTCAGATCGCCAGGCGCAGTGAGTTGCATACCTTCGCCGTTATCCCCAAGC
GTTGGGTAGTGGAACGCAGCTTTGCCTGGTTGGAGAAGAATCGACGGCTGTGGAAGAACTGTGAGCGCTTACTCAACACCAGCTTGCAGTTTATTCACCT
GGCCTTCCTGGCCCTTCTACTCAAAAGACTTTGAACAGGTTC
GCTGGAGAGCGCGCGCAGGAAGACCAGCCCTCGACGAATAGATCTGTACGATGTTTTTTGTGCGGTGCTGTATTTGCTCAAGAGCGGCTGCCAGTGGCGA
GCCCTGCCTAATGACTTTCCCAAGTGGCGCACTGTCCACTCATACTGGGCCATCTGGAGTGAGCCGCGCGAAGGAGGCAGCCTGCTGGAGCAGGCATTAA
AAAAATCAGGTTGGCGAGGTTCGTACCAGACAGGGGCGCAACGCCTGCACGTCATTCTTGATCGTGGACGCGCAGAGCGTGAAGAACACGGACACGGCGA
GCCAGAAGGGCTATGACGCAGGCAAGAAGGTATCGGGCATCAAGCGTCATATCGCGGTCGATACCCAGGGTTTGCCGCATGCCATTGCGGTGACGACAGC
GGAAGTGACCGATCGCAAAGGTGCGTTGCAGGCTTTCCGGCGTTGTCGTTCAGGCCTGGGCCGGGTGGTGGCGGTGTTGGCGGACAGCGGTTATGTGGGA
GAGCCGTTTGCTCAAGGTGTACGCGATGTGCTGAGCGAGACTGTCGCGGTTCAGATCGCCAGGCGCAGTGAGTTGCATACCTTCGCCGTTATCCCCAAGC
GTTGGGTAGTGGAACGCAGCTTTGCCTGGTTGGAGAAGAATCGACGGCTGTGGAAGAACTGTGAGCGCTTACTCAACACCAGCTTGCAGTTTATTCACCT
GGCCTTCCTGGCCCTTCTACTCAAAAGACTTTGAACAGGTTC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 793 bp | 264 aa | 42 | 834 | + | No |
Chemistry : DDE
ORF sequence :
MRRSYPSDISREQFELIKPLLESARRKTSPRRIDLYDVFCAVLYLLKSGCQWRALPNDFPKWRTVHSYWAIWSEPREGGSLLEQALKKSGWRGSYQTGAQ
RACTSFLIVDAQSVKNTDTASQKGYDAGKKVSGIKRHIAVDTQGLPHAIAVTTAEVTDRKGALQAFRRCRSGLGRVVAVLADSGYVGEPFAQGVRDVLSE
TVAVQIARRSELHTFAVIPKRWVVERSFAWLEKNRRLWKNCERLLNTSLQFIHLAFLALLLKRL
RACTSFLIVDAQSVKNTDTASQKGYDAGKKVSGIKRHIAVDTQGLPHAIAVTTAEVTDRKGALQAFRRCRSGLGRVVAVLADSGYVGEPFAQGVRDVLSE
TVAVQIARRSELHTFAVIPKRWVVERSFAWLEKNRRLWKNCERLLNTSLQFIHLAFLALLLKRL
Blast result :
Comments
The ORF has been reconstructed in silico because there is a mutation generating a frameshift at position 343 in the DNA .
The protein seems to be active (11 copies of ISNieu4 on this genome), suggesting a possible sequencing error.
ISNieu4 is 86% aa similar to IS1480a, and 87% to ISXo3.
The protein seems to be active (11 copies of ISNieu4 on this genome), suggesting a possible sequencing error.
ISNieu4 is 86% aa similar to IS1480a, and 87% to ISXo3.
References
1] Stein,L.Y., Arp,D.J., Berube,P.M., Chain,P.S., Hauser,L., Jetten,M.S., Klotz,M.G., Larimer,F.W., Norton,J.M., Op den Camp,H.J., Shin,M. and Wei,X. (2007) Environ. Microbiol. 9 (12), 2993-3007.
2] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina del Rio,T., Hammon,N., Israni,S., Pitluck,S., Di Bartolo,G., Chain,P., Malfatti,S., Shin,M., Vergez,L., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Kim,E., Arp,D., Klotz,M., Norton,J., Stein,L., Sayavedra-Soto,L., Hommes,N. and Richardson,P. (2006) Direct submission GenBank.
2] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina del Rio,T., Hammon,N., Israni,S., Pitluck,S., Di Bartolo,G., Chain,P., Malfatti,S., Shin,M., Vergez,L., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Kim,E., Arp,D., Klotz,M., Norton,J., Stein,L., Sayavedra-Soto,L., Hommes,N. and Richardson,P. (2006) Direct submission GenBank.