ISPst8
- Family IS5
- Group IS427
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| EF648210 | ND | Pseudomonas stutzeri | Pseudomonas stutzeri ATCC 17595 |
DNA section
IS Length : 911 bp
Ends
IR Length : 21/22
IRL : GGTTCTGTACGAAAAGTCTGAGGAGATAGAATCGGTGCCATGACAGGCTG
IRR : GGTTCTGTACGAAAAGTACTGACGCAAACACCGCAGCGTGCAAGCCAGCG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CTGCGCTCGC | TA | GGACGGGCTAAA | 2 |
DNA sequence
GGTTCTGTACGAAAAGTCTGAGGAGATAGAATCGGTGCCATGACAGGCTGGAGATCCGCATGGCAAAGCGTTACGAGCTCCCTGATGCGGCTTGGGAGCT
GATCAAGGATCTGGTTTCCCCCGAACAGAAGATGGGACGACCTCGCAGTGATGACCGATTGATGCTCAATGGCATCCTCTGGGTCTTGTGCTCAGGTGCA
GCCTGGCGTGACATGCCGGAGCGCTTCGGCCCGTGGTCGACGGTCTACCAGCGTTTTCGCGACTGGCGCGACGATGGCACATTCGATCAAGTGCTTGAGC
GTTTGCACATCCGGCTGAACCAGGAAGGCCTGATTGATCTGGATACCTGGATGATCGACTCAACAGCGGTGCGAGCAACCCGAGCTTCTTCTGGAGCCGG
GAAAAAGGGGGGCCAGAAGAGCCGGTAGACCATGCGTTGGGACGTAGCCGAGGCGGCCTGACCACCAAGATCCACATGGTCTGCGACGCCAACGGCATAC
CTCTACGCTTTTTGCTGACACCGGGTCAGGCCAGCGACATATCGAATGCCCAGGTACTTCTCGATCAGGTACGCATCCCCGGCAAGCAAGGTCGGCCCCG
CAAGCGCTGCCGCTGGTTGCTGGCAGGCAAGGGTTACGATGCCGAACAGCTGCGTCAGTACTGCGACCGTTACCGAATGCAGCCTGTGATTCCACTGCGA
ACCATGAAACGTAAGCCCAAGCCAGGATTACCAAGGCTGTTCGATCGCCCGAAATACCGGCAGCGCAACATCATCGAGCGCATGTTCGGCTGGCTAAAGG
AGAGCCGCAGGATCGGCACTCGTTACGACAAGCTGGCAAGAAGTTTTGCCGCGATGGTCACGCTGGCTTGCACGCTGCGGTGTTTGCGTCAGTACTTTTC
GTACAGAACC
GATCAAGGATCTGGTTTCCCCCGAACAGAAGATGGGACGACCTCGCAGTGATGACCGATTGATGCTCAATGGCATCCTCTGGGTCTTGTGCTCAGGTGCA
GCCTGGCGTGACATGCCGGAGCGCTTCGGCCCGTGGTCGACGGTCTACCAGCGTTTTCGCGACTGGCGCGACGATGGCACATTCGATCAAGTGCTTGAGC
GTTTGCACATCCGGCTGAACCAGGAAGGCCTGATTGATCTGGATACCTGGATGATCGACTCAACAGCGGTGCGAGCAACCCGAGCTTCTTCTGGAGCCGG
GAAAAAGGGGGGCCAGAAGAGCCGGTAGACCATGCGTTGGGACGTAGCCGAGGCGGCCTGACCACCAAGATCCACATGGTCTGCGACGCCAACGGCATAC
CTCTACGCTTTTTGCTGACACCGGGTCAGGCCAGCGACATATCGAATGCCCAGGTACTTCTCGATCAGGTACGCATCCCCGGCAAGCAAGGTCGGCCCCG
CAAGCGCTGCCGCTGGTTGCTGGCAGGCAAGGGTTACGATGCCGAACAGCTGCGTCAGTACTGCGACCGTTACCGAATGCAGCCTGTGATTCCACTGCGA
ACCATGAAACGTAAGCCCAAGCCAGGATTACCAAGGCTGTTCGATCGCCCGAAATACCGGCAGCGCAACATCATCGAGCGCATGTTCGGCTGGCTAAAGG
AGAGCCGCAGGATCGGCACTCGTTACGACAAGCTGGCAAGAAGTTTTGCCGCGATGGTCACGCTGGCTTGCACGCTGCGGTGTTTGCGTCAGTACTTTTC
GTACAGAACC
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 369 bp | 122 aa | 60 | 428 | + | No |
Description : First part of the transposase
ORF sequence :
MAKRYELPDAAWELIKDLVSPEQKMGRPRSDDRLMLNGILWVLCSGAAWRDMPERFGPWSTVYQRFRDWRDDGTFDQVLERLHIRLNQEGLIDLDTWMID
STAVRATRASSGAGKKGGQKSR
STAVRATRASSGAGKKGGQKSR
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 574 bp | 191 aa | 338 | 911 | + | No |
Description : Second part of the transposase
ORF sequence :
SGYLDDRLNSGASNPSFFWSREKGGPEEPVDHALGRSRGGLTTKIHMVCDANGIPLRFLLTPGQASDISNAQVLLDQVRIPGKQGRPRKRCRWLLAGKGY
DAEQLRQYCDRYRMQPVIPLRTMKRKPKPGLPRLFDRPKYRQRNIIERMFGWLKESRRIGTRYDKLARSFAAMVTLACTLRCLRQYFSYRT
DAEQLRQYCDRYRMQPVIPLRTMKRKPKPGLPRLFDRPKYRQRNIIERMFGWLKESRRIGTRYDKLARSFAAMVTLACTLRCLRQYFSYRT
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 852 bp | 284 aa | 60 | 911 | + | Yes |
Chemistry : DDE
ORF sequence :
MAKRYELPDAAWELIKDLVSPEQKMGRPRSDDRLMLNGILWVLCSGAAWRDMPERFGPWSTVYQRFRDWRDDGTFDQVLERLHIRLNQEGLIDLDTWMID
STAVRATRASSGAGKKGGPEEPVDHALGRSRGGLTTKIHMVCDANGIPLRFLLTPGQASDISNAQVLLDQVRIPGKQGRPRKRCRWLLAGKGYDAEQLRQ
YCDRYRMQPVIPLRTMKRKPKPGLPRLFDRPKYRQRNIIERMFGWLKESRRIGTRYDKLARSFAAMVTLACTLRCLRQYFSYRT
STAVRATRASSGAGKKGGPEEPVDHALGRSRGGLTTKIHMVCDANGIPLRFLLTPGQASDISNAQVLLDQVRIPGKQGRPRKRCRWLLAGKGYDAEQLRQ
YCDRYRMQPVIPLRTMKRKPKPGLPRLFDRPKYRQRNIIERMFGWLKESRRIGTRYDKLARSFAAMVTLACTLRCLRQYFSYRT
Blast result :
Comments
ISPst8 was observed within an integron gene cassette in the chromosome of Pseudomonas stutzeri ATCC 17595. Th e stop codon is in the DR.
ISPst8 is 92%(ORFA) and 89% (ORFB) aa similar to ISPsy15.
The third ORF is a putative transposase reconstructed in silico by possible -1 frameshift.
ISPst8 is 92%(ORFA) and 89% (ORFB) aa similar to ISPsy15.
The third ORF is a putative transposase reconstructed in silico by possible -1 frameshift.
References
1] Sasha Tetu (2007) Direct submission .