ISGur15
- Family IS21
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_009483 | ND | Geobacter uraniumreducens | Geobacter uraniumreducens Rf4 |
DNA section
IS Length : 2390 bp
Ends
IR Length : 12/15
IRL : TGACCCACTTTCGAGCATAATCGGCGTCCAAAATTGACCCACCCCAGCAC
IRR : TGACCCACCATCGGCGTCCAATTTTGACCCACCCCAGGTAGTAGTTTTCA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TTCGAGCATA | GGTGGGTCAA | 0 |
DNA sequence
TGACCCACTTTCGAGCATAATCGGCGTCCAAAATTGACCCACCCCAGCACTCTGCTCCATACTCTCTGATTTCATCAGAGAGAGGAGAAACAAAGGAGAT
GCTGAACGTGGAAACCATCCGTAAAGTCCGTCAGGCTCATTTTAGAGACGGCAAGGGAATTCGTGAGATAACCCGCGAGTTCAACCTGTCCCGCAACACC
GTCCGATCCATTATCCGTAGCGGCATAACCGACCAAAAGTATGAGCGGAGTGAACAGCCCCGCCCCAAGCTCGGCTCTTTTGTTGAACGGCTGTCAGAAT
GGTTGAAGGAAGATCGTGATAAACCCGTTAAACATCGTCGTCGCGCCCAGATTCTTTTTGAGCAGTTGCAGCGGGAAGGGTATACCGGAGGCTATGATGC
TGTACGTCGTTATGTGGGCACTTGGAAGGAAGACGATGGCACGGCTTTAGTCAAAGCCTTCATCCCTCTCGAATGGGAACCCGGTGACGCCTTCCAGTTC
GACTGGAGTTATGAGAAGGTGGAGCTGGGTGGTATGCCGGTAGAAGTGAAGGTATCCCATTTCCGTCTGTGCCACAGCCGCAAGCCGTTCTGTGTCGCGT
ATACCCGTGAGAGTTTGGAGATGGTCCTTGATGCCCATTCCCGCGCTTTCGAATTCTTTGGCGGTATCTGCAAACGCGGCATCTACGACAACCTTAAAAC
GGTTGTCACCAAGGTACTGATGGGCAAGGACCGGGTCTTCAACCGGCGCTTCCAGAACCTGGCCTCCCACTACCTGTTCGAACCGGTGGCCTGTACCCCT
GCCGCCGGCTGGGAGAAGGGACAGGTGGAGAATCAGGTTGGCGTGGTCCGGCACCGCTTCTTCGTTCAGCGCCGACGCTTTGCCTCCCTTGAGGAGCTGA
ACGAATGGCTGGAAGGTGAATGCCGCAATCACGCCGCCACAGCAAAGCATCCTGAGTTCAAGGAGAGAACCATTGACCAGGTCTTTGCCGAAGAGAAGAC
CAAACTCTTGGCACTTCCGGTATCTCCTTTCGACGGCTACCAGGAAAGCTTTGTCCGTGTCTCATCGCAACTGCTGATCAGCTTTGATCGCAACCGTTAC
AGCGTCAATGCTATGGCCGTAGGCAAATCGGTCTCGGTGCGGGCATACGCCAACCATGTCAGCATGATTCTGAATGGCACAGTTGTCGGCGTTCACCAGC
GCAATCTTGGCCGTGACAAAGTCATCTATGATCCCTGGCACTATCTGGCTGTTCTGGAGCAGAAGCCGGGAGCATTACGAAACGGAGCGCCCTTCAAACG
ATGGGATCTGCCGGAAGCTATGAGCGAAGTCAGGACAATATTGGAAGGACGTTCTGGTGGTGACCGCCAGTTCGTCGGCATCCTCTCTGTCGTCAGCCGC
TACGGACTGGAAGCAGTAGCCACTGCCTGTACGCAGGCTCTCTCCGATAAAACGGTCAGCAGTGATGTCATCCTCAGCATCCTGTCCCGCACACACGACG
AGCCGCAACCGGAACCAGTGAAACTGTCAGCACAACTGCCGCTTCTCAAGCTGGTCCCGGTGGTTGATTGTTACCGCTATGACCGCCTCCTCTCGGGAGG
TGCGCATGGTACTGCGTGACCGCCTGATGGAGGCGATGGAAACACTTGGGCTGTACGGCATGAAGGCCACCTTCGACGAGCTACTGGCGGCAGGCATAAA
AAGCCGGGCCACTCCGGAGAAGATCCTCTGTGATCTTCTGGAAACGGAGATGGCAGAGCGCAAGGCTAGAAGCATCCGCTATCGCATGGGCTTGGCGAAG
TTCCCCGTGGATAAGGATCTGGACCGGTTTGAATTTACGGCCTCACCGGTCAATGAGATGCAGGTGAGGCACCTTTACAGTGGTAACTTTCTCGCTGATC
ACACCAACATCATCATGGTCGGTGGCACCGGTACCGGCAAAACACACCTGGCCATAGCGATTGCCAAGCAGAGCATCAGAAACGGAAGGAAAGCCAGGTT
CTTCAACGTACTGGATCTGGTAAACCAGCTTGAGCAGGAGAAACTGGACAACAGAGGTGGTAAACTGGCGGAACACCTGGTCAGGCATGACCTGGTCATA
CTTGATGAACTGGGATACCTGCCGTTCTCCAAGAATGGCGGCCAGCTTCTGTTCCACCTGATCTCGAAGCTCTACGAGAGGACGTCGCTCATCATCACCA
CAAACCTGACCTTCGGTGAATGGCCGCAGGTGTTCAGCGATGGCAAGATGACGACAGCTCTCCTGGACAGGGTCACGCATCACTGCGAGATAATCGAAAC
CGGAAACGAAAGCTGGAGAATCAGAACGAGGATCAATAACTGAAAACTACTACCTGGGGTGGGTCAAAATTGGACGCCGATGGTGGGTCA
GCTGAACGTGGAAACCATCCGTAAAGTCCGTCAGGCTCATTTTAGAGACGGCAAGGGAATTCGTGAGATAACCCGCGAGTTCAACCTGTCCCGCAACACC
GTCCGATCCATTATCCGTAGCGGCATAACCGACCAAAAGTATGAGCGGAGTGAACAGCCCCGCCCCAAGCTCGGCTCTTTTGTTGAACGGCTGTCAGAAT
GGTTGAAGGAAGATCGTGATAAACCCGTTAAACATCGTCGTCGCGCCCAGATTCTTTTTGAGCAGTTGCAGCGGGAAGGGTATACCGGAGGCTATGATGC
TGTACGTCGTTATGTGGGCACTTGGAAGGAAGACGATGGCACGGCTTTAGTCAAAGCCTTCATCCCTCTCGAATGGGAACCCGGTGACGCCTTCCAGTTC
GACTGGAGTTATGAGAAGGTGGAGCTGGGTGGTATGCCGGTAGAAGTGAAGGTATCCCATTTCCGTCTGTGCCACAGCCGCAAGCCGTTCTGTGTCGCGT
ATACCCGTGAGAGTTTGGAGATGGTCCTTGATGCCCATTCCCGCGCTTTCGAATTCTTTGGCGGTATCTGCAAACGCGGCATCTACGACAACCTTAAAAC
GGTTGTCACCAAGGTACTGATGGGCAAGGACCGGGTCTTCAACCGGCGCTTCCAGAACCTGGCCTCCCACTACCTGTTCGAACCGGTGGCCTGTACCCCT
GCCGCCGGCTGGGAGAAGGGACAGGTGGAGAATCAGGTTGGCGTGGTCCGGCACCGCTTCTTCGTTCAGCGCCGACGCTTTGCCTCCCTTGAGGAGCTGA
ACGAATGGCTGGAAGGTGAATGCCGCAATCACGCCGCCACAGCAAAGCATCCTGAGTTCAAGGAGAGAACCATTGACCAGGTCTTTGCCGAAGAGAAGAC
CAAACTCTTGGCACTTCCGGTATCTCCTTTCGACGGCTACCAGGAAAGCTTTGTCCGTGTCTCATCGCAACTGCTGATCAGCTTTGATCGCAACCGTTAC
AGCGTCAATGCTATGGCCGTAGGCAAATCGGTCTCGGTGCGGGCATACGCCAACCATGTCAGCATGATTCTGAATGGCACAGTTGTCGGCGTTCACCAGC
GCAATCTTGGCCGTGACAAAGTCATCTATGATCCCTGGCACTATCTGGCTGTTCTGGAGCAGAAGCCGGGAGCATTACGAAACGGAGCGCCCTTCAAACG
ATGGGATCTGCCGGAAGCTATGAGCGAAGTCAGGACAATATTGGAAGGACGTTCTGGTGGTGACCGCCAGTTCGTCGGCATCCTCTCTGTCGTCAGCCGC
TACGGACTGGAAGCAGTAGCCACTGCCTGTACGCAGGCTCTCTCCGATAAAACGGTCAGCAGTGATGTCATCCTCAGCATCCTGTCCCGCACACACGACG
AGCCGCAACCGGAACCAGTGAAACTGTCAGCACAACTGCCGCTTCTCAAGCTGGTCCCGGTGGTTGATTGTTACCGCTATGACCGCCTCCTCTCGGGAGG
TGCGCATGGTACTGCGTGACCGCCTGATGGAGGCGATGGAAACACTTGGGCTGTACGGCATGAAGGCCACCTTCGACGAGCTACTGGCGGCAGGCATAAA
AAGCCGGGCCACTCCGGAGAAGATCCTCTGTGATCTTCTGGAAACGGAGATGGCAGAGCGCAAGGCTAGAAGCATCCGCTATCGCATGGGCTTGGCGAAG
TTCCCCGTGGATAAGGATCTGGACCGGTTTGAATTTACGGCCTCACCGGTCAATGAGATGCAGGTGAGGCACCTTTACAGTGGTAACTTTCTCGCTGATC
ACACCAACATCATCATGGTCGGTGGCACCGGTACCGGCAAAACACACCTGGCCATAGCGATTGCCAAGCAGAGCATCAGAAACGGAAGGAAAGCCAGGTT
CTTCAACGTACTGGATCTGGTAAACCAGCTTGAGCAGGAGAAACTGGACAACAGAGGTGGTAAACTGGCGGAACACCTGGTCAGGCATGACCTGGTCATA
CTTGATGAACTGGGATACCTGCCGTTCTCCAAGAATGGCGGCCAGCTTCTGTTCCACCTGATCTCGAAGCTCTACGAGAGGACGTCGCTCATCATCACCA
CAAACCTGACCTTCGGTGAATGGCCGCAGGTGTTCAGCGATGGCAAGATGACGACAGCTCTCCTGGACAGGGTCACGCATCACTGCGAGATAATCGAAAC
CGGAAACGAAAGCTGGAGAATCAGAACGAGGATCAATAACTGAAAACTACTACCTGGGGTGGGTCAAAATTGGACGCCGATGGTGGGTCA
Protein section
ORF number : 2
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1521 bp | 506 aa | 99 | 1619 | + | No |
Chemistry : DDE
ORF sequence :
MLNVETIRKVRQAHFRDGKGIREITREFNLSRNTVRSIIRSGITDQKYERSEQPRPKLGSFVERLSEWLKEDRDKPVKHRRRAQILFEQLQREGYTGGYD
AVRRYVGTWKEDDGTALVKAFIPLEWEPGDAFQFDWSYEKVELGGMPVEVKVSHFRLCHSRKPFCVAYTRESLEMVLDAHSRAFEFFGGICKRGIYDNLK
TVVTKVLMGKDRVFNRRFQNLASHYLFEPVACTPAAGWEKGQVENQVGVVRHRFFVQRRRFASLEELNEWLEGECRNHAATAKHPEFKERTIDQVFAEEK
TKLLALPVSPFDGYQESFVRVSSQLLISFDRNRYSVNAMAVGKSVSVRAYANHVSMILNGTVVGVHQRNLGRDKVIYDPWHYLAVLEQKPGALRNGAPFK
RWDLPEAMSEVRTILEGRSGGDRQFVGILSVVSRYGLEAVATACTQALSDKTVSSDVILSILSRTHDEPQPEPVKLSAQLPLLKLVPVVDCYRYDRLLSG
GAHGTA
AVRRYVGTWKEDDGTALVKAFIPLEWEPGDAFQFDWSYEKVELGGMPVEVKVSHFRLCHSRKPFCVAYTRESLEMVLDAHSRAFEFFGGICKRGIYDNLK
TVVTKVLMGKDRVFNRRFQNLASHYLFEPVACTPAAGWEKGQVENQVGVVRHRFFVQRRRFASLEELNEWLEGECRNHAATAKHPEFKERTIDQVFAEEK
TKLLALPVSPFDGYQESFVRVSSQLLISFDRNRYSVNAMAVGKSVSVRAYANHVSMILNGTVVGVHQRNLGRDKVIYDPWHYLAVLEQKPGALRNGAPFK
RWDLPEAMSEVRTILEGRSGGDRQFVGILSVVSRYGLEAVATACTQALSDKTVSSDVILSILSRTHDEPQPEPVKLSAQLPLLKLVPVVDCYRYDRLLSG
GAHGTA
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 738 bp | 245 aa | 1606 | 2343 | + | No |
AG : IS21 helper
ORF sequence :
MVLRDRLMEAMETLGLYGMKATFDELLAAGIKSRATPEKILCDLLETEMAERKARSIRYRMGLAKFPVDKDLDRFEFTASPVNEMQVRHLYSGNFLADHT
NIIMVGGTGTGKTHLAIAIAKQSIRNGRKARFFNVLDLVNQLEQEKLDNRGGKLAEHLVRHDLVILDELGYLPFSKNGGQLLFHLISKLYERTSLIITTN
LTFGEWPQVFSDGKMTTALLDRVTHHCEIIETGNESWRIRTRINN
NIIMVGGTGTGKTHLAIAIAKQSIRNGRKARFFNVLDLVNQLEQEKLDNRGGKLAEHLVRHDLVILDELGYLPFSKNGGQLLFHLISKLYERTSLIITTN
LTFGEWPQVFSDGKMTTALLDRVTHHCEIIETGNESWRIRTRINN
Blast result :
Comments
ISGur15 is 55% ORFA and 70% ORFB aa similar to IS1600.
References
1] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavinadel Rio,T., Hammon,N., Israni,S., Dalin,E., Tice,H., Pitluck,S.,Chertkov,O., Brettin,T., Bruce,D., Han,C., Schmutz,J., Larimer,F.,Land,M., Hauser,L., Kyrpides,N., Mikhailova,N., Shelobolina,E.,Aklujkar,M., Lovley,D. and Richardson,P.(2007) Direct submission GenBank.