ISGur10
- Family IS3
- Group IS3
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_009483 | ND | Geobacter uraniumreducens | Geobacter uraniumreducens Rf4 |
DNA section
IS Length : 2157 bp
Ends
IR Length : 14/14
IRL : TGAGACACTTACCTCCCCTTAAATTACTGTAGAGGGTGGGGAGGAAAGAA
IRR : TGAGACACTTACCTGTCGAATTTAGTGTAGCGAATTTTAGCTAGTGGCCG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GTGCCAATAT | AAG | TCATTGACAG | 3 |
DNA sequence
TGAGACACTTACCTCCCCTTAAATTACTGTAGAGGGTGGGGAGGAAAGAAAGGATATGACCATCAGTAATGTGAAGCGCATCCCGGTAATTGCAGGAGCC
CGTAAGGCGAGTGCAATTACCGGGATGGCCCCCGAAGATTTAACTCGGCAACAGCAGGAATTGACTACCTCACCGCCTGACCCGGAGGTAGCGGAAAAGC
CTGTACGCAGGCGTTTCACGGCTAAGTACAAACTTGACATCCTAAACCAAGTCGACTCTTGCACCAAGCCCGGTAGCCTGGCAGCGCTATTACGTCGAGA
GGGGCTTTATGCTTCCAACCTCAACACCTGGCGCCGCCAGCGGGATGAAGGAAGCCTTGCGGCGCTTACGCCCAAGCAGAGGGGCCGTAAAGCGCTAAAG
CCCGATCCCATGGCCGTCGAGAACGAGCGACTGCGCAAGGAGATTACCCGCCTTGCCAAACGCCTCAAACAGGCCGAGTTGATCATCGATGTCCAAAAAA
AAGTCTCGCAGATCCTGGGGATCACCTTGGATACGCCGCCAGAGAGCGAGAGCGACTGATGGAAGCTGTCGAAATACTTGCCCCTGATGTGGGCACCGAT
CCTGCCTGTAAAGCTCTTGGTGTTTCCCGTGCCGGGCTGTATCGCAGGCGCGCAATCAGCAAGACAGCGCCGCGTGTACCCCAAAAGCGCCCTGTGCCGC
CACGGACCTTGCCATCAGAGGAAAGGCAGGCAGTACTCGATATTCTGCATTCCGAGCGCTTTCAGGACAAGGCACCCCATGAGGTGTATGCAACGCTTTT
GGACGAAGGGGCCTATCACTGCTCGATCCGCACGATGTATCGAATTCTCGAAGAAAACGCCGAAGTCAAAGAGCGGAGAAATCAGTTGCGGCATCCCGAC
TACAAAAAGCCTGAGCTTCTGGCAACGGCCCCAAATCAAGTCTGGTCGTGGGATATTACCAAATTGATGGGGCCTGCTAAATGGTCCTATTTTTACCTCT
ATGTGATTCTCGATATCTTCAGCCGCTATGTGGTCGGCTGGATGGTTGCAACCTGCGAGTCGTCGGAACTGGCCAAACGGCTGTTCAAAGAAACGTGTGA
GAAACAAGTCATCCAGAAAGGGCAGTTAACGATCCACGCCGATCGCGGCTCTTCCATGAAATCCAAGCCGGTAGCTTTTCTGTTTGCTGATCTGGGGATC
ACTAAAACGCACTCAAGGCCCTATACGAGCGACGACAACCCATATTCGGAAGCCCAATTTAAAACGCTGAAATATCAGCCTGATTTCCCGGAAAGATTCG
GCAGTATAGAAGACGCAAGATCGTTCTGCCAAACCTTTTTCCCCTGGTATAACCAGGAACACAAGCATTCCGGCATCAGCCTACTTACACCTGAAGTAGT
ACATTACGGCAGAGCTGAGGAAGTCATCCGATCAAGGCAAACCGTGTTGACTGCTGCTTATGCAGCCCATCCTGAACGCTTCGTCAGGAAAATACCATTA
CATAAACCGTTACCGGAAGCGGCGTGGATCAACCCGCCAAAACCGGCCACTAGCTAAAATTCGCTACACTAAATTCGACAGGTAAGTGTCTCA
CGTAAGGCGAGTGCAATTACCGGGATGGCCCCCGAAGATTTAACTCGGCAACAGCAGGAATTGACTACCTCACCGCCTGACCCGGAGGTAGCGGAAAAGC
CTGTACGCAGGCGTTTCACGGCTAAGTACAAACTTGACATCCTAAACCAAGTCGACTCTTGCACCAAGCCCGGTAGCCTGGCAGCGCTATTACGTCGAGA
GGGGCTTTATGCTTCCAACCTCAACACCTGGCGCCGCCAGCGGGATGAAGGAAGCCTTGCGGCGCTTACGCCCAAGCAGAGGGGCCGTAAAGCGCTAAAG
CCCGATCCCATGGCCGTCGAGAACGAGCGACTGCGCAAGGAGATTACCCGCCTTGCCAAACGCCTCAAACAGGCCGAGTTGATCATCGATGTCCAAAAAA
AAGTCTCGCAGATCCTGGGGATCACCTTGGATACGCCGCCAGAGAGCGAGAGCGACTGATGGAAGCTGTCGAAATACTTGCCCCTGATGTGGGCACCGAT
CCTGCCTGTAAAGCTCTTGGTGTTTCCCGTGCCGGGCTGTATCGCAGGCGCGCAATCAGCAAGACAGCGCCGCGTGTACCCCAAAAGCGCCCTGTGCCGC
CACGGACCTTGCCATCAGAGGAAAGGCAGGCAGTACTCGATATTCTGCATTCCGAGCGCTTTCAGGACAAGGCACCCCATGAGGTGTATGCAACGCTTTT
GGACGAAGGGGCCTATCACTGCTCGATCCGCACGATGTATCGAATTCTCGAAGAAAACGCCGAAGTCAAAGAGCGGAGAAATCAGTTGCGGCATCCCGAC
TACAAAAAGCCTGAGCTTCTGGCAACGGCCCCAAATCAAGTCTGGTCGTGGGATATTACCAAATTGATGGGGCCTGCTAAATGGTCCTATTTTTACCTCT
ATGTGATTCTCGATATCTTCAGCCGCTATGTGGTCGGCTGGATGGTTGCAACCTGCGAGTCGTCGGAACTGGCCAAACGGCTGTTCAAAGAAACGTGTGA
GAAACAAGTCATCCAGAAAGGGCAGTTAACGATCCACGCCGATCGCGGCTCTTCCATGAAATCCAAGCCGGTAGCTTTTCTGTTTGCTGATCTGGGGATC
ACTAAAACGCACTCAAGGCCCTATACGAGCGACGACAACCCATATTCGGAAGCCCAATTTAAAACGCTGAAATATCAGCCTGATTTCCCGGAAAGATTCG
GCAGTATAGAAGACGCAAGATCGTTCTGCCAAACCTTTTTCCCCTGGTATAACCAGGAACACAAGCATTCCGGCATCAGCCTACTTACACCTGAAGTAGT
ACATTACGGCAGAGCTGAGGAAGTCATCCGATCAAGGCAAACCGTGTTGACTGCTGCTTATGCAGCCCATCCTGAACGCTTCGTCAGGAAAATACCATTA
CATAAACCGTTACCGGAAGCGGCGTGGATCAACCCGCCAAAACCGGCCACTAGCTAAAATTCGCTACACTAAATTCGACAGGTAAGTGTCTCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 435 bp | 144 aa | 125 | 559 | + | No |
Description : First part of the transposase
ORF sequence :
MAPEDLTRQQQELTTSPPDPEVAEKPVRRRFTAKYKLDILNQVDSCTKPGSLAALLRREGLYASNLNTWRRQRDEGSLAALTPKQRGRKALKPDPMAVEN
ERLRKEITRLAKRLKQAELIIDVQKKVSQILGITLDTPPESESD
ERLRKEITRLAKRLKQAELIIDVQKKVSQILGITLDTPPESESD
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1167 bp | 388 aa | 391 | 1557 | + | No |
Description : Second part of the transposase
ORF sequence :
SAKARSHGRRERATAQGDYPPCQTPQTGRVDHRCPKKSLADPGDHLGYAARERERLMEAVEILAPDVGTDPACKALGVSRAGLYRRRAISKTAPRVPQKR
PVPPRTLPSEERQAVLDILHSERFQDKAPHEVYATLLDEGAYHCSIRTMYRILEENAEVKERRNQLRHPDYKKPELLATAPNQVWSWDITKLMGPAKWSY
FYLYVILDIFSRYVVGWMVATCESSELAKRLFKETCEKQVIQKGQLTIHADRGSSMKSKPVAFLFADLGITKTHSRPYTSDDNPYSEAQFKTLKYQPDFP
ERFGSIEDARSFCQTFFPWYNQEHKHSGISLLTPEVVHYGRAEEVIRSRQTVLTAAYAAHPERFVRKIPLHKPLPEAAWINPPKPATS
PVPPRTLPSEERQAVLDILHSERFQDKAPHEVYATLLDEGAYHCSIRTMYRILEENAEVKERRNQLRHPDYKKPELLATAPNQVWSWDITKLMGPAKWSY
FYLYVILDIFSRYVVGWMVATCESSELAKRLFKETCEKQVIQKGQLTIHADRGSSMKSKPVAFLFADLGITKTHSRPYTSDDNPYSEAQFKTLKYQPDFP
ERFGSIEDARSFCQTFFPWYNQEHKHSGISLLTPEVVHYGRAEEVIRSRQTVLTAAYAAHPERFVRKIPLHKPLPEAAWINPPKPATS
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1433 bp | 477 aa | 125 | 1557 | + | Yes |
Chemistry : DDE
ORF sequence :
MAPEDLTRQQQELTTSPPDPEVAEKPVRRRFTAKYKLDILNQVDSCTKPGSLAALLRREGLYASNLNTWRRQRDEGSLAALTPKQRGRKALKPDPMAVEN
ERLRKEITRLAKRLKQAELIIDVQKKSLADPGDHLGYAARERERLMEAVEILAPDVGTDPACKALGVSRAGLYRRRAISKTAPRVPQKRPVPPRTLPSEE
RQAVLDILHSERFQDKAPHEVYATLLDEGAYHCSIRTMYRILEENAEVKERRNQLRHPDYKKPELLATAPNQVWSWDITKLMGPAKWSYFYLYVILDIFS
RYVVGWMVATCESSELAKRLFKETCEKQVIQKGQLTIHADRGSSMKSKPVAFLFADLGITKTHSRPYTSDDNPYSEAQFKTLKYQPDFPERFGSIEDARS
FCQTFFPWYNQEHKHSGISLLTPEVVHYGRAEEVIRSRQTVLTAAYAAHPERFVRKIPLHKPLPEAAWINPPKPATS
ERLRKEITRLAKRLKQAELIIDVQKKSLADPGDHLGYAARERERLMEAVEILAPDVGTDPACKALGVSRAGLYRRRAISKTAPRVPQKRPVPPRTLPSEE
RQAVLDILHSERFQDKAPHEVYATLLDEGAYHCSIRTMYRILEENAEVKERRNQLRHPDYKKPELLATAPNQVWSWDITKLMGPAKWSYFYLYVILDIFS
RYVVGWMVATCESSELAKRLFKETCEKQVIQKGQLTIHADRGSSMKSKPVAFLFADLGITKTHSRPYTSDDNPYSEAQFKTLKYQPDFPERFGSIEDARS
FCQTFFPWYNQEHKHSGISLLTPEVVHYGRAEEVIRSRQTVLTAAYAAHPERFVRKIPLHKPLPEAAWINPPKPATS
Blast result :
Comments
ISGur10 is 55 % (ORFA) aa similar to IS1087 and 71% (ORFB) aa similar to IS1141. The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavinadel Rio,T., Hammon,N., Israni,S., Dalin,E., Tice,H., Pitluck,S.,Chertkov,O., Brettin,T., Bruce,D., Han,C., Schmutz,J., Larimer,F.,Land,M., Hauser,L., Kyrpides,N., Mikhailova,N., Shelobolina,E.,Aklujkar,M., Lovley,D. and Richardson,P. (2007) Direct submission GenBank.