ISfinder Mirror Site

ISGur8

Family IS21
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_009483	ND	Geobacter uraniumreducens	Geobacter uraniumreducens Rf4

DNA section

IS Length : 2775 bp

Ends

IR Length : 27/48

IRL : TGATCAGCGACAATTAAAACTCCCGCCTGACGACAATTATAATTCCCGGT
IRR : TGATCAACGTCAACTATTTTTCCCGGTCAACGACAATTATAATTCCCGTT

Insertion site

Left flank	Direct repeat	Right flank	DR Length
CGTCTGTGAT	GCTATCA	GGATCGCCGC	7

DNA sequence

TGATCAGCGACAATTAAAACTCCCGCCTGACGACAATTATAATTCCCGGTAATTTGTGGAATTGTAAACTATCCACCATCTTTGGAAAAAAGGATGGCAC
GGATGGTAACTGATCAACAGGTAAGGAGGTTATTCAAGCTGATGCAAACGGAAAAAACACAAGCTGTAGCCGCAGCCAAATCTGGCATGGACGAAAAAAC
TGCTCGAAAGTATCTAAAGACAGGCAGGTTGCCAAGTGAGCTCATAAAAGAGCGAACATGGCGGACCCGGCCAGATCCTTTCGAAGACATTTGGGATGAA
GTCCGCGAAAACCTTGCTTCAAATCCGGGATTTGAAGCAAAAACTCTTTTCGATGACTTGCAGAACCGCTTTCCCGGCAAGTTTTCGGACGGGCAATTAC
GGACTCTCCAGCGACGGATTAAGCGCTGGCGTGCCCTGGAGGGGCCTTCCCAGGAAATCTTTTTCCCCCAACTGCACCGCCCCGGCGAACTGGCGCAGTC
GGATTACACACACATGGAGAAACTGGGAATCACCATCGCCAATCAGCCATTTGACCATCTGCTGTATCATTTTGTGCTGACCTACTCCAACTGGGAAACG
GGCAGCGTCTGCTTCTCAGAAAGCTTCGAAAGCCTCAGCGAAGGGCTACAGGCCGCATTATGGGAACTGGGCGGTGTGCCGCAGACTCACCAGACAGACC
GGCTGACGGCAGCGGTGCAAAAAGCCCTGCACCCGGATGAATTTACCCAACGCTACCAGGCGCTGCTCAAACACTACGGACTCACCGGGCGCAAAACACA
GGCAGCAAGCCCCAATGAAAATGGAGATGTGGAGCAGAGCAACTATCGGCTCAAAAAAGCGGTCCAGCAGGCTCTGTTGCTGCGAGGAGGCGCTGATTTC
GCCACTCTCGACGACTACAAGCAATTTCTGAAAAAACTCTTCGCCAGATTAAACAGCGGCCGCCGTGAACGGTTTCTGGAAGAACAACGGGTCTTGCACA
GCCTCCCGGATCAGCGCCTGGAAAGCTGCAAGCGCCTATCCGTCAGCGTCGGCCCGAGCAGCACCATCCGCGTCAACCACAACGTCTACTCGGTTGAAAG
CAGATTGATCGGTGAAACCATTCAGGTTCACCTTTTTGCCGAACATCTCGATCTTTTCTATGCCCAAAAGTGTGTGGACACGCTTCCCCGCTTGCGCGGG
GAAAATCGGCATCTGATCAATTACCGGCACATGATCGACCAACTGGAGCGTAAGCCGGGGGCGTTCGAGAACTACCGCTACCGGGAAGAGATGTTTCCCG
GCAGCTATTTCCGGCTTGCCTATGATGAACTCAAAGAGCGGCATACGCCGCAGCAGGCCGTCAGGGAATACCTAAAAATATTGCGCATGGCAGCCATGGA
GAGCGAAACAGCCGTAGGAGCAGCCCTTTCTGATCTCTGCGGCCGGCAACCGATCACTGCCTGTGCCGTGGAAGAAATCCTTCACAAACAACAGCCGCCG
TCCCCTGTTGCCGAAGTCAGTATCGCTCAGGTGGACCTGGCCGCTTATGATCGTCTCCTGTTGAATGAGGAGGTGTCATATGCAGTGTGAAGGTGAAAAA
GCCGGCCGCCTGGACCGGCGCCTCAAAGAGTTACACTTGCCCACCGTGCGGCGCTGCTATCAGGACGAGGCCATCAATGCCCGGCGAGACAACTGGGATT
ACGAAACCTATCTTTATGAATTAGCCGAACGCGAATGTGAGGACCGGCAAAACAATCGAATTGCCCGCCTGCTCAGGGAATCAAAACTTCCCTTGGAAAA
AAGTCTGAAAGCCTTTGACCGCAAGCGTCTGCCCCGGAAGGTGGATGCACAGCTCAATCTCCTGTTGAAAGGAGACTTTCTTGAGCATAGAGAGAACGTA
CTGGCATTTGGCAATCCCGGCAGCGGCAAGACTCATCTCGTATGCGCGATCGCTCAGGAGTTGATTTACCAGGGACGGCAGATCCGTTTTATCCCCTGCA
ACCTGCTGGTGCAGGAACTTCTGATCGCGAAACGGGACCTGAAACTGGCGCGTGTTCTCAAGCAGTACGCGAAGTATGAAGCCCTGATCATTGACGACAT
CGGCTACGTGCAGCAAAGCCGGGAAGAGATGGAAGTTCTCTTCACTCTTCTTGCCGACCGCTATGAGCGCAGCAGCATAATGATCACCAGCAATTTGCCG
TTCTCCAAATGGGAGCAGATTTTTAAAGATCCCATGACCACGGCAGCAGCAATTGACCGATTGGTCCATCACAGCGTTATCCTGGAGCTGAACTTATCCA
GTTACAGATTGGAGCAATCCCAAAAATCAAAGGAAACCGTTGCTGTCCAATCGGCTCAAGCAGAGCCGCAGGAACAAAACACGGCGGGTTAAGGAGCTGA
GAACGTATGGAATTCACGGTATATAATCCTCAAAAAGGCCGACTGGAAACGCTCGATGTGGATCTCACGGCAGAGAATACGACCTGGTTCGAAAACAGTG
AGAATACCCGTGTTGTCGCCACTATCACGGATTTCGACGGCGGGCTTCTCATCAAAGCCCTCGATAATACGTATCCAATCTGGCTGTATGACATATCGAG
GGCGGATATTGACTATAGCCAGAAAAAGGCAAAAGAGCTTATGCAGCAGCACGAGTAGCGGTTCGCTTGGTAGCGGAAACGCAGGTCGCCCTACGGGCTC
CCTCCGTTTCTGCTACCGCACTTAAAACGGGAATTATAATTGTCGTTGACCGGGAAAAATAGTTGACGTTGATCA

Protein section

ORF number : 2

ORF 1

Length		Begin	End	Strand	Fusion ORF
1449 bp	482 aa	142	1590	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MQTEKTQAVAAAKSGMDEKTARKYLKTGRLPSELIKERTWRTRPDPFEDIWDEVRENLASNPGFEAKTLFDDLQNRFPGKFSDGQLRTLQRRIKRWRALE
GPSQEIFFPQLHRPGELAQSDYTHMEKLGITIANQPFDHLLYHFVLTYSNWETGSVCFSESFESLSEGLQAALWELGGVPQTHQTDRLTAAVQKALHPDE
FTQRYQALLKHYGLTGRKTQAASPNENGDVEQSNYRLKKAVQQALLLRGGADFATLDDYKQFLKKLFARLNSGRRERFLEEQRVLHSLPDQRLESCKRLS
VSVGPSSTIRVNHNVYSVESRLIGETIQVHLFAEHLDLFYAQKCVDTLPRLRGENRHLINYRHMIDQLERKPGAFENYRYREEMFPGSYFRLAYDELKER
HTPQQAVREYLKILRMAAMESETAVGAALSDLCGRQPITACAVEEILHKQQPPSPVAEVSIAQVDLAAYDRLLLNEEVSYAV

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
813 bp	270 aa	1580	2392	+	No

ORF function : Accessory Gene
AG : IS21 helper

ORF sequence :

MQCEGEKAGRLDRRLKELHLPTVRRCYQDEAINARRDNWDYETYLYELAERECEDRQNNRIARLLRESKLPLEKSLKAFDRKRLPRKVDAQLNLLLKGDF
LEHRENVLAFGNPGSGKTHLVCAIAQELIYQGRQIRFIPCNLLVQELLIAKRDLKLARVLKQYAKYEALIIDDIGYVQQSREEMEVLFTLLADRYERSSI
MITSNLPFSKWEQIFKDPMTTAAAIDRLVHHSVILELNLSSYRLEQSQKSKETVAVQSAQAEPQEQNTAG

Blast result :

Comments

ISGur8 is 55% (ORFA) aa similar to ISEc10 and 55% istB transposition helper aa similar to ISLxx1.

References

1] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavinadel Rio,T., Hammon,N., Israni,S., Dalin,E., Tice,H., Pitluck,S.,Chertkov,O., Brettin,T., Bruce,D., Han,C., Schmutz,J., Larimer,F.,Land,M., Hauser,L., Kyrpides,N., Mikhailova,N., Shelobolina,E.,Aklujkar,M., Lovley,D. and Richardson,P.(2007) Direct submission GenBank.