ISGur6
- Family IS3
- Group IS3
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_009483 | ND | Geobacter uraniireducens | Geobacter uraniireducens Rf4 |
DNA section
IS Length : 1667 bp
Ends
IR Length : 20/29
IRL : CCCCCTGTGTCAGGATAGTTGTCGCCTCTCTTCCGAACTTTTGTGTTAAC
IRR : CGGTCGTTGTCAAGGAAGTTGTCGCATCTTCTATGCTGCTCTGGCTTGAG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| ATTGGCTTCG | GATG | GCGTAATTTC | 4 |
| CTGATCTTCA | TGAT | GAACACAGAT | 4 |
DNA sequence
CCCCCTGTGTCAGGATAGTTGTCGCCTCTCTTCCGAACTTTTGTGTTAACCTGCGGGGCAACAGAGAGAGGGACCATGGCCACCAAATATTCCGAAGAAT
TCAAGTCGAGCATTATTGCCAGGCTTCTGCCTCCGCATAATGTCAGCGTTCCTGACATGGTAAAAGAGACCGGGGTTCCTAAAGACACCCTGTACACTTG
GAGGAATCAATACCGCAACAGGCAGGTTAATGCCGAGGCGAGCGGAAATCATCCGGCAGCCAGCCTAAACAACGAAGAGAAGTTGGCCATCGTCATCGAA
TCCGCCAGTCTCACTGAAGTTGAACTGGGTGAATATTGCCGACGCAAGGGACTCTACCCTGAGCAGATCGCCGGTTGGAAGAACGGCTTCGTACTCGGAT
CATCTGCCCCCCTCAACAAGGCCGAACGCGAGCAAATGAGGGAACAGACAGCGACCATTAAGCAGCTTGAGAAAGAGCTGAACCGCAAGGATAAAGCATT
GGCCGAGACTGCAGCCCTGCTGATTCTTCAAAAAAAGTTCCAGGCGCTCTGGGAGGAGCCCGAGGCAGAAAAATCGACCTCCCGGCGCGCCAAGAACTGA
TTGCCCTGATAGACGAAGCCTGTGCCGGTGGTGCACGTCTGGGTAGGGCCTGCGAGGCTGTTCTTCTTTCGCCCCGCACCGTGCAACGTTGGCATCTTCC
TGACGAAATACCCTCAGATGGGCGCAAGGCCGCGTCTCGCGATCGGGCACCGGCCAATAAGCTTTCTGAAACGGAGCGGGTCACAATCCTGGCGACTGCC
AACCAGGAGAAATTCGCCAGCCTGCCGCCCAGCCAGATAGTTCCTAAATTGGCCGACGACGGGATCTTCCTCGCTTCTGAGTCAACCTTCTACCGAACCT
TACGGGCAGAGAAGCAGCTGGCACACCGGGGCCGTACCAAGGCTGCAAGACACAAGCGCCCAGATGCCGTGCTGGCCACCGAGCCGAACCAACTCTGGAG
CTGGGATATTACATACTTGAGCACTACCGTTAAGGGGCTGTACTTTTACCTCTACCTGATCATGGATGTCTTTAGCCGCAAGATCGTCGGTTGGGAAGTC
TATGCGGAGGAATCCGCCGATCATGCCGCCATAACGTTGCGCAAGGCCTATCTGCGTGAAGGCGTCGCCGGTCGCACCCTGATACTTCACTCCGACAACG
GCTCGCCCATGAAAGGCGCTACCATGTTGGGAACTATGCAGAAGCTTGGGGTAATGCCGTCCTTCAGTCGGCCCTCTGTCAGCAATGACAACCCATACTC
AGAGTCACTGTTCAAAACCTTGAAGTATCACCCCGGCCTACCGGAGAAACCTTTCGATACGTTGGACGAGGCACGGCACTGGGTTGCCGGTTTCCAGCAT
TGGTACAACGAGGAACATCAGCACAGTGCCATAAAATTCGTAACGCCGGGTCAGCGCCACCGGGGCGATGACAAAGAGATACTCAACAAACGAGCGCTAC
TTTACGCGATGGCGAGGGCCGCAATGCCGGAACGATGGTCGGGTGCTTGCCGGAACTGGACTCGACCAGCAGAAGTTTCCTTGAACCCCCAAAAATCGGC
TCAGAACGGTACGCCGTCTCAAGCCAGAGCAGCATAGAAGATGCGACAACTTCCTTGACAACGACCG
TCAAGTCGAGCATTATTGCCAGGCTTCTGCCTCCGCATAATGTCAGCGTTCCTGACATGGTAAAAGAGACCGGGGTTCCTAAAGACACCCTGTACACTTG
GAGGAATCAATACCGCAACAGGCAGGTTAATGCCGAGGCGAGCGGAAATCATCCGGCAGCCAGCCTAAACAACGAAGAGAAGTTGGCCATCGTCATCGAA
TCCGCCAGTCTCACTGAAGTTGAACTGGGTGAATATTGCCGACGCAAGGGACTCTACCCTGAGCAGATCGCCGGTTGGAAGAACGGCTTCGTACTCGGAT
CATCTGCCCCCCTCAACAAGGCCGAACGCGAGCAAATGAGGGAACAGACAGCGACCATTAAGCAGCTTGAGAAAGAGCTGAACCGCAAGGATAAAGCATT
GGCCGAGACTGCAGCCCTGCTGATTCTTCAAAAAAAGTTCCAGGCGCTCTGGGAGGAGCCCGAGGCAGAAAAATCGACCTCCCGGCGCGCCAAGAACTGA
TTGCCCTGATAGACGAAGCCTGTGCCGGTGGTGCACGTCTGGGTAGGGCCTGCGAGGCTGTTCTTCTTTCGCCCCGCACCGTGCAACGTTGGCATCTTCC
TGACGAAATACCCTCAGATGGGCGCAAGGCCGCGTCTCGCGATCGGGCACCGGCCAATAAGCTTTCTGAAACGGAGCGGGTCACAATCCTGGCGACTGCC
AACCAGGAGAAATTCGCCAGCCTGCCGCCCAGCCAGATAGTTCCTAAATTGGCCGACGACGGGATCTTCCTCGCTTCTGAGTCAACCTTCTACCGAACCT
TACGGGCAGAGAAGCAGCTGGCACACCGGGGCCGTACCAAGGCTGCAAGACACAAGCGCCCAGATGCCGTGCTGGCCACCGAGCCGAACCAACTCTGGAG
CTGGGATATTACATACTTGAGCACTACCGTTAAGGGGCTGTACTTTTACCTCTACCTGATCATGGATGTCTTTAGCCGCAAGATCGTCGGTTGGGAAGTC
TATGCGGAGGAATCCGCCGATCATGCCGCCATAACGTTGCGCAAGGCCTATCTGCGTGAAGGCGTCGCCGGTCGCACCCTGATACTTCACTCCGACAACG
GCTCGCCCATGAAAGGCGCTACCATGTTGGGAACTATGCAGAAGCTTGGGGTAATGCCGTCCTTCAGTCGGCCCTCTGTCAGCAATGACAACCCATACTC
AGAGTCACTGTTCAAAACCTTGAAGTATCACCCCGGCCTACCGGAGAAACCTTTCGATACGTTGGACGAGGCACGGCACTGGGTTGCCGGTTTCCAGCAT
TGGTACAACGAGGAACATCAGCACAGTGCCATAAAATTCGTAACGCCGGGTCAGCGCCACCGGGGCGATGACAAAGAGATACTCAACAAACGAGCGCTAC
TTTACGCGATGGCGAGGGCCGCAATGCCGGAACGATGGTCGGGTGCTTGCCGGAACTGGACTCGACCAGCAGAAGTTTCCTTGAACCCCCAAAAATCGGC
TCAGAACGGTACGCCGTCTCAAGCCAGAGCAGCATAGAAGATGCGACAACTTCCTTGACAACGACCG
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 525 bp | 174 aa | 76 | 600 | + | No |
Description : First part of the transposase
ORF sequence :
MATKYSEEFKSSIIARLLPPHNVSVPDMVKETGVPKDTLYTWRNQYRNRQVNAEASGNHPAASLNNEEKLAIVIESASLTEVELGEYCRRKGLYPEQIAG
WKNGFVLGSSAPLNKAEREQMREQTATIKQLEKELNRKDKALAETAALLILQKKFQALWEEPEAEKSTSRRAKN
WKNGFVLGSSAPLNKAEREQMREQTATIKQLEKELNRKDKALAETAALLILQKKFQALWEEPEAEKSTSRRAKN
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1143 bp | 380 aa | 495 | 1637 | + | No |
Description : Second part of the transposase
ORF sequence :
SIGRDCSPADSSKKVPGALGGARGRKIDLPARQELIALIDEACAGGARLGRACEAVLLSPRTVQRWHLPDEIPSDGRKAASRDRAPANKLSETERVTILA
TANQEKFASLPPSQIVPKLADDGIFLASESTFYRTLRAEKQLAHRGRTKAARHKRPDAVLATEPNQLWSWDITYLSTTVKGLYFYLYLIMDVFSRKIVGW
EVYAEESADHAAITLRKAYLREGVAGRTLILHSDNGSPMKGATMLGTMQKLGVMPSFSRPSVSNDNPYSESLFKTLKYHPGLPEKPFDTLDEARHWVAGF
QHWYNEEHQHSAIKFVTPGQRHRGDDKEILNKRALLYAMARAAMPERWSGACRNWTRPAEVSLNPQKSAQNGTPSQARAA
TANQEKFASLPPSQIVPKLADDGIFLASESTFYRTLRAEKQLAHRGRTKAARHKRPDAVLATEPNQLWSWDITYLSTTVKGLYFYLYLIMDVFSRKIVGW
EVYAEESADHAAITLRKAYLREGVAGRTLILHSDNGSPMKGATMLGTMQKLGVMPSFSRPSVSNDNPYSESLFKTLKYHPGLPEKPFDTLDEARHWVAGF
QHWYNEEHQHSAIKFVTPGQRHRGDDKEILNKRALLYAMARAAMPERWSGACRNWTRPAEVSLNPQKSAQNGTPSQARAA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1562 bp | 520 aa | 76 | 1637 | + | Yes |
Chemistry : DDE
ORF sequence :
MATKYSEEFKSSIIARLLPPHNVSVPDMVKETGVPKDTLYTWRNQYRNRQVNAEASGNHPAASLNNEEKLAIVIESASLTEVELGEYCRRKGLYPEQIAG
WKNGFVLGSSAPLNKAEREQMREQTATIKQLEKELNRKDKALAETAALLILQKKVPGALGGARGRKIDLPARQELIALIDEACAGGARLGRACEAVLLSP
RTVQRWHLPDEIPSDGRKAASRDRAPANKLSETERVTILATANQEKFASLPPSQIVPKLADDGIFLASESTFYRTLRAEKQLAHRGRTKAARHKRPDAVL
ATEPNQLWSWDITYLSTTVKGLYFYLYLIMDVFSRKIVGWEVYAEESADHAAITLRKAYLREGVAGRTLILHSDNGSPMKGATMLGTMQKLGVMPSFSRP
SVSNDNPYSESLFKTLKYHPGLPEKPFDTLDEARHWVAGFQHWYNEEHQHSAIKFVTPGQRHRGDDKEILNKRALLYAMARAAMPERWSGACRNWTRPAE
VSLNPQKSAQNGTPSQARAA
WKNGFVLGSSAPLNKAEREQMREQTATIKQLEKELNRKDKALAETAALLILQKKVPGALGGARGRKIDLPARQELIALIDEACAGGARLGRACEAVLLSP
RTVQRWHLPDEIPSDGRKAASRDRAPANKLSETERVTILATANQEKFASLPPSQIVPKLADDGIFLASESTFYRTLRAEKQLAHRGRTKAARHKRPDAVL
ATEPNQLWSWDITYLSTTVKGLYFYLYLIMDVFSRKIVGWEVYAEESADHAAITLRKAYLREGVAGRTLILHSDNGSPMKGATMLGTMQKLGVMPSFSRP
SVSNDNPYSESLFKTLKYHPGLPEKPFDTLDEARHWVAGFQHWYNEEHQHSAIKFVTPGQRHRGDDKEILNKRALLYAMARAAMPERWSGACRNWTRPAE
VSLNPQKSAQNGTPSQARAA
Blast result :
Comments
ISGur6 is 66% (ORFA) aa similar to IS1240 and 69% (ORFB) aa similar to ISPpr7. The third ORF is a putative ORFAB transposase reconstructed in silico by -1 frameshift.
File updated: September 13 2013. Extremities were modified, the proposed ends for this sequence are based on the similarities with closely relative elements.
File updated: September 13 2013. Extremities were modified, the proposed ends for this sequence are based on the similarities with closely relative elements.
References
1] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavinadel Rio,T., Hammon,N., Israni,S., Dalin,E., Tice,H., Pitluck,S.,Chertkov,O., Brettin,T., Bruce,D., Han,C., Schmutz,J., Larimer,F.,Land,M., Hauser,L., Kyrpides,N., Mikhailova,N., Shelobolina,E.,Aklujkar,M., Lovley,D. and Richardson,P. (2007) Direct submission GenBank.