ISGme7

  • Family IS3
  • Group IS407
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_007517 ND Geobacter metallireducens
Geobacter metallireducens GS-15
DNA section
IS Length : 1305 bp

Ends


IR Length : 34/45

IRL : TGACCTTCCCCCCATCATCCAGCCATTTGGAAGTTAGTGATTTGGCCTTG
IRR : TGACCTTCCCCCCAGAAACTGAACCATTGCAAACTTAGTGATTTCTGTCT

Insertion site


Left flankDirect repeatRight flankDR Length
CCTGGGACAGGGTAACTAGGATTGA0

DNA sequence

TGACCTTCCCCCCATCATCCAGCCATTTGGAAGTTAGTGATTTGGCCTTGTGACCTTCCCCCTGTAAACTGATCCAAAAAATTGTGTTAGTCTTTCGGAA
TCCGAAAGGGGGCATAATGAAAAAATCTAGGTTTACAGAGGAGCAGGTAGCTGCGGCAATAAGACAGGCAGAATCAGGAGTTCCAGTTGCTGAGATCTGC
CGTCGTCTTGGAGTCACTGAAGTCACCTTTTACCGCTGGAAGAAGCAATTTGCCGGCATGGGTGTGACCGAACTGAGAAAGCTACGTCAACTCGAAGACG
AGAATCGCAAGCTCAAGTCATTAGTTGCCGATCTGACCCTGGACAAGCACATGCTTCAGGAGGTCATTGCATAAAAGCTGTGAAGCCGGCGCGCAAGCGG
CAGCTGGTTGAGTTCCTCCGAGTGGCTTTCAAGGTGAGTGAGCGCCATGCCTGCCGGCTGATACGATTGCAACGCTCAAGTCAGCGCTACCGGCATCGCC
GTAATGAACAGATTGCCTTGCGCATTCGGCTCAAAGAGCATGCTGCCGCTCGTCCTCGCTTTGGGTACTTGCGGTTACACATTCTGCTGCTTCGGGAAGG
CTGGCAGATCAACCGTAAACGCGTTTATCGATTGTACCGGTTGGAATGCCTTAACTGCGCCACAAGAAGCCTAAAAAGCGAATTTCGGCCTTGAGGGTTG
CTCATCCTGCACCGCAGAGACCTAATGAGTGTTGGAGCATGGACTTTGTTTCTGATAGCCTCCATGATGGTAGGCGATTTCGGGCCTTAACGATAGTCGA
TAACGTAAGCCGTGTTAGTCCGGCAATTGAGGTCGGAAACTCGCTCTCGGGGCGCCGGGTAGTCGATGTGCTTGAAGGTCTGGCCTATACATATGGCCTA
CCGAAGGTAATCCAAGTCGACAATGGACCGGAGTTCACCTCGAAGGCGCTGGATGAATGGGCTCACCGGAGAAAAATCAAGCTTCAGGTCAGTCGGCCAG
GCAAACCAACCGACAATCCGTTCATCGAGAGCTTCAACGGTCGGCTCCGGCAGGAATGTCTTGATCAAAGCTGGTTTATTTCAATAGAAGACGCCCAGAT
GAAAACCGAAGAATGGCGTGTAGATTACAACGAAAACCGGCCGCACAGTTCACTTGAAAACAGGGCTCCTAGCGACTTCCTGGATGCTTGGAACCAAAAT
CAACAAACCGAAAAACTAACACATCTTTTGGACCAAAAAACGGGGTAAGGTCACTAGACAGAAATCACTAAGTTTGCAATGGTTCAGTTTCTGGGGGGAA
GGTCA
Recoding section
  • Recoding by frameshift
  • Frame
  • Type
  • Experimentally demonstrated

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
578 bp192 aa117694+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MKKSRFTEEQVAAAIRQAESGVPVAEICRRLGVTEVTFYRWKKQFAGMGVTELRKLRQLEDENRKLKSLVADLTLDKHMLQEVIAIKAVKPARKRQLVEF
LRVAFKVSERHACRLIRLQRSSQRYRHRRNEQIALRIRLKEHAAARPRFGYLRLHILLLREGWQINRKRVYRLYRLECLNCATRSLKSEFRP

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
594 bp197 aa6551248+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

LRHKKPKKRISALRVAHPAPQRPNECWSMDFVSDSLHDGRRFRALTIVDNVSRVSPAIEVGNSLSGRRVVDVLEGLAYTYGLPKVIQVDNGPEFTSKALD
EWAHRRKIKLQVSRPGKPTDNPFIESFNGRLRQECLDQSWFISIEDAQMKTEEWRVDYNENRPHSSLENRAPSDFLDAWNQNQQTEKLTHLLDQKTG

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1132 bp377 aa1171248+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MKKSRFTEEQVAAAIRQAESGVPVAEICRRLGVTEVTFYRWKKQFAGMGVTELRKLRQLEDENRKLKSLVADLTLDKHMLQEVIAIKAVKPARKRQLVEF
LRVAFKVSERHACRLIRLQRSSQRYRHRRNEQIALRIRLKEHAAARPRFGYLRLHILLLREGWQINRKRVYRLYRLECLNCATRSLKKRISALRVAHPAP
QRPNECWSMDFVSDSLHDGRRFRALTIVDNVSRVSPAIEVGNSLSGRRVVDVLEGLAYTYGLPKVIQVDNGPEFTSKALDEWAHRRKIKLQVSRPGKPTD
NPFIESFNGRLRQECLDQSWFISIEDAQMKTEEWRVDYNENRPHSSLENRAPSDFLDAWNQNQQTEKLTHLLDQKTG

 

Blast result :
Comments
ISGme7 is 71% aa similar to ISYpe1. There is a stop codon in ORFA, it is reconstructed by joigning 117-371; 372-694.
The third ORF is a putative ORFAB transposase reconstructed by possible -1 frameshift.
There is only one copy on the chromosome.
References
1] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina,T., Hammon,N., Israni,S., Pitluck,S., Di Bartolo,G., Chain,P., Schmutz,J., Larimer,F., Land,M., Kyrpides,N., Ivanova,N. and Richardson,P. (2005) Direct submission GenBank
2] Muktak Aklujkar (2007) Direct submission.