ISfinder Mirror Site

ISGme4

Family IS21
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_007517	ND	Geobacter metallireducens	Geobacter metallireducens GS-15

DNA section

IS Length : 1976 bp

Ends

IR Length : 23/29

IRL : TGTTAGCGGCAATGTAAAAATGACCCACCTCGGAAATTGAAAATTGACCC
IRR : TGTCAACGGCAATCGAAAATTGACCCACTTTACCGGTTGATCGGCAATTG

Insertion site

Left flank	Direct repeat	Right flank	DR Length
TTGCGTTGGATGTCACGA	ATCACC	CGGCCAAGGTACAG	6

DNA sequence

TGTTAGCGGCAATGTAAAAATGACCCACCTCGGAAATTGAAAATTGACCCACTCTTCCTGATAACCTAGCCGACCTGGAGGGTCGGCATGATCGGAAAGG
AAGAGTGTATGGAAGTCAGAATTCTCAAGAAACAGGGCAAAAGCATCCGGGAGATCTCGCGGATCACCGGGCTATGCCGAAACACGGTCAGGAAGTTCCT
CAGATCAACGGCCGATCCCAGATACAAAGAACGGGCGAAGCGGCCGGGGAAGCTAGATCCATTTAAAGCCTTTCTCGAAGAGCGGGTGAAGGCGGCGCTG
CCACACCGGATTCCTGCTCCGGTGCTTGCTCGGGAAATTGCCAGCTTTGGCTACGATGGCTGCGAGCGCACCGTAAGAACGTTTTTGGCGACACTTTACT
CCCGTGTCACTCCGGAGCCGGTTGTCCGGTTTGAAACGGAGCCCGGCAAGCAGATGCAAGCCGACTGGTGCGAATTGCGCAGGGGTAAACACCCATTGTA
CGCGTTCGTTGGAACCCTTGGTTTCAGCCGCGATTCGTTCGTCGTCTTCACCACCAGCCAGGCCTTTGATGTCCTTCGTGAGTGCCATGAGATGGCATTT
TCCTTCTTCGGCGGCGTCCCGCGTGAAGTGCTCTATGACAACATGAAGACTGTGGTTCTGGAGCGAAACGCCTTCGGCGAGGGCAAGCACCGCTTCCACT
CCGGCCTGTGGGACACAGCCAAGCATTTCGGGTTTATCCCCCGGCTGTGCAAGCCGTACCGCGCCAAGACCAAGGGAAAGGTCGAGCGGTTCAACCGCTA
CCTGCGGTACAGCTTCTACTATCCGCTGGTCTCGCGCCTTAAGCAGGCAGGATTAACTCTCGATGTCGCGACCGCCAACATCGAGGTCCGTAACTGGCTT
AATGACGTAGCCAACTGCCGAATCCATGGCGAGACCAGCGAGCGCCCCTGCGATCGTCTCAAGATCGAGCGCGCGGCAATGTCGCCGCTGCCGCCAAGGG
TTAAGGTTGAGCAGGGCAAGCCGGAAGTCATCACTCCCATGCCGTGGCCGGTGATCCCACTTCAGCGCCCCGCCTCCTTCTATGATCAGATCCTTCAGGA
GGGGCGGCTATGAGCGACATCCAGCACCAGCGTATGGTCACCCTCTGCGACGACTTGAAGTTTCTGGCGGTGACCGATGTGTACGCCGACCTTGCCGATG
CTGCGGCAAAGCAGGAGTCGTCATACATCGACTATCTCGAGCAGGTACTCAAGGCCGAGAACGACGTCCGGCAGGGGCGCTCGCGCCACACCATGGCAAA
GCTCGCGGGCTTTCCCGCGATCAAGACCCTTGAGGATTATGACTTCGATTTTGCCACCGGCGCCCCGAAGCAGCGAATCCTGGACCTATCGGCAATGGCG
TTTCTGGAGCGTCGGGAGAATGTAATCCTGCTCGGTCCCAGCGGTACCGGCAAGACTCACCTCGCCATTGCCCTCGGCTACCGGGCAACCCAGTGCGGCG
TGAAGGTGCGCTTCATTTCCGCTGCGGACCTGATGCTGCAACTCGAAAGCGCCCAGCGCCAGGGGCGGTACAAGGAAGTAATGCGGCGCAGTGTCCTGGG
GCCGAGACTACTCATCATCGACGAGATTGGATACCTTCCGTTCAGCGAAACACAGGCAAACCTGTTCTTTCAGGTGATCGCCAAAAGGTACGAAACAGGT
TCCGTCATCCTCACCTCGAACCTGAGCTTTGGGGAATGGGAACAGGCTTTCGGCGGCAACACGGCACTCACATCAGCAATGCTCGACCGGTTGCTGCACC
ACGCCCATGTCATTCAGATCAGGGGTGACAGCTACCGATTGAAAGAGAAGCGCCGAGCTGGCATTCTCGGGCAGCAACTGCCGACACCCCAGATGGATGA
TTAACCCAGGAAGGTGGGTCATTTTTCAATTGCCGATCAACCGGTAAAGTGGGTCAATTTTCGATTGCCGTTGACA

Protein section

ORF number : 2

ORF 1

Length		Begin	End	Strand	Fusion ORF
1026 bp	341 aa	88	1113	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MIGKEECMEVRILKKQGKSIREISRITGLCRNTVRKFLRSTADPRYKERAKRPGKLDPFKAFLEERVKAALPHRIPAPVLAREIASFGYDGCERTVRTFL
ATLYSRVTPEPVVRFETEPGKQMQADWCELRRGKHPLYAFVGTLGFSRDSFVVFTTSQAFDVLRECHEMAFSFFGGVPREVLYDNMKTVVLERNAFGEGK
HRFHSGLWDTAKHFGFIPRLCKPYRAKTKGKVERFNRYLRYSFYYPLVSRLKQAGLTLDVATANIEVRNWLNDVANCRIHGETSERPCDRLKIERAAMSP
LPPRVKVEQGKPEVITPMPWPVIPLQRPASFYDQILQEGRL

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
795 bp	264 aa	1110	1904	+	No

ORF function : Accessory Gene
AG : IS21 helper

ORF sequence :

MSDIQHQRMVTLCDDLKFLAVTDVYADLADAAAKQESSYIDYLEQVLKAENDVRQGRSRHTMAKLAGFPAIKTLEDYDFDFATGAPKQRILDLSAMAFLE
RRENVILLGPSGTGKTHLAIALGYRATQCGVKVRFISAADLMLQLESAQRQGRYKEVMRRSVLGPRLLIIDEIGYLPFSETQANLFFQVIAKRYETGSVI
LTSNLSFGEWEQAFGGNTALTSAMLDRLLHHAHVIQIRGDSYRLKEKRRAGILGQQLPTPQMDD

Blast result :

Comments

ISGme4 is 96% (ORFA) and 99% (ORFB) aa similar to ISGsu6. There are 8 copies on chromosome.

References

1] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina,T., Hammon,N., Israni,S., Pitluck,S., Di Bartolo,G., Chain,P., Schmutz,J., Larimer,F., Land,M., Kyrpides,N., Ivanova,N. and Richardson,P. (2005) Direct submission GenBank
2] Muktak Aklujkar (2007) Direct submission.