ISSod21
- Family IS110
- Group IS1111
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| AE014300 | ND | Shewanella oneidensis | Shewanella oneidensis MR-1 |
DNA section
IS Length : 1345 bp
Ends
IR Length : 13
IRL : gatgtgATGGACGCACCTCCTTTCTAGCGTCGCTGTACCAAACTTTAGGT
IRR : --atatATGGACGCACCTCATGATGCTATAGCCAATTAAAGGGGACAGTC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CCGAGGCTTT | GTTTAAACTG | 0 |
DNA sequence
GATGTGATGGACGCACCTCCTTTCTAGCGTCGCTGTACCAAACTTTAGGTACAAGTCAGAGCAGGAGTTAGTAACATATCTATCAAGGTTGTTGGTATTG
ATTTAGCCAAAAATTCTTTTCAAGTATGCGTATTGAGTATCTCTGGGTCGATTTTACCCAATCGGAAAGTAAATCGAGGTTCTCTACTCGATACTATCCG
TCAATTGCCTGAAAACACGACTATAGCCATGGAGTTGTGCTACATCGCATCACAGGGGAAGAATCTTCCAATAACAGGGGTTTAAGGTTGCCCTCATCCC
CGCACAACATGTTAAACCATTCGTAGGAAGGTAAAAGAATGATGCCAACGATGCGAGGGCAATATGTGAGGCATTTTCTCGCCCAGACATTCACTTTGTA
CCCGTTAAATCGGTAGAGCAGCAAGATCTCAAATCTCTCCGAGGTGTTCGAGCACGTTTAGTTGAACAACGAACTGCACTTGCTAATCAAATTAGAGGCT
TATCTGCCGAATATGGCATTATATTTCCGTTATCAATGAAGGCTCTGAACAGTTATTTACCTCTGATAATCGAAGACGCAGAGAATGAACTATCTCATGT
AATGAGGGGGGACTGCACACGTTGTATAGCGACCTTATAGGTATCGGTGAAAGGATTGATGAGCTCACCTGTAAAATCACGATGCTTGGACAACAGCACC
CTCGATATGATGCCATCAAGAGTATTCCTAGTTTTGCTCCTATTTTAACAGCCGCACTGCTCAGTGAGGTTGGTACCGGGAACCAATTTCAAAATGGGCG
GCAGTTTTCAGCTTGGTGTGGATTTAGTCCCTAAACAACATAGCTCTGCCGGGAAAAGTTCTCTCGGTTCTTTATCAAAGAGCGGTAATCGTGATTTAAG
AACCTTACTAATCCATGGTGCACGGGCGGTTATGCGGTACGGGATAAACAGAACCGATGCCATGGGCGATTGGCTAAGAAGTATCATTGGAAGACGTGGT
AGAGCAAAAGCCATAGTCGCAATGGCAAACAAGCTTGGTAGGATAGCTTGGCGGGTTTTAGCGACAGATAGCAGTTGCGATATCCATAAGGCTTTTAAGG
CTGTATAACTGGGGAATTATGATTTTGAATGGCAACTGAGTTTGCAATTACTGATGAAGAAACGGTGAGCCATCCTTACTGCACTCTGATTAGGAAACAG
GTCATACAGACCGGGGCTTTCAATAGACAGTAAGGTGCGTATTACATCATGGCGCAGGCCATTTAGTGGCCAGAGATGACGTCGGATATATGTTAGCGAC
TGTCCCCTTTAATTGGCTATAGCATCATGAGGTGCGTCCATATAT
ATTTAGCCAAAAATTCTTTTCAAGTATGCGTATTGAGTATCTCTGGGTCGATTTTACCCAATCGGAAAGTAAATCGAGGTTCTCTACTCGATACTATCCG
TCAATTGCCTGAAAACACGACTATAGCCATGGAGTTGTGCTACATCGCATCACAGGGGAAGAATCTTCCAATAACAGGGGTTTAAGGTTGCCCTCATCCC
CGCACAACATGTTAAACCATTCGTAGGAAGGTAAAAGAATGATGCCAACGATGCGAGGGCAATATGTGAGGCATTTTCTCGCCCAGACATTCACTTTGTA
CCCGTTAAATCGGTAGAGCAGCAAGATCTCAAATCTCTCCGAGGTGTTCGAGCACGTTTAGTTGAACAACGAACTGCACTTGCTAATCAAATTAGAGGCT
TATCTGCCGAATATGGCATTATATTTCCGTTATCAATGAAGGCTCTGAACAGTTATTTACCTCTGATAATCGAAGACGCAGAGAATGAACTATCTCATGT
AATGAGGGGGGACTGCACACGTTGTATAGCGACCTTATAGGTATCGGTGAAAGGATTGATGAGCTCACCTGTAAAATCACGATGCTTGGACAACAGCACC
CTCGATATGATGCCATCAAGAGTATTCCTAGTTTTGCTCCTATTTTAACAGCCGCACTGCTCAGTGAGGTTGGTACCGGGAACCAATTTCAAAATGGGCG
GCAGTTTTCAGCTTGGTGTGGATTTAGTCCCTAAACAACATAGCTCTGCCGGGAAAAGTTCTCTCGGTTCTTTATCAAAGAGCGGTAATCGTGATTTAAG
AACCTTACTAATCCATGGTGCACGGGCGGTTATGCGGTACGGGATAAACAGAACCGATGCCATGGGCGATTGGCTAAGAAGTATCATTGGAAGACGTGGT
AGAGCAAAAGCCATAGTCGCAATGGCAAACAAGCTTGGTAGGATAGCTTGGCGGGTTTTAGCGACAGATAGCAGTTGCGATATCCATAAGGCTTTTAAGG
CTGTATAACTGGGGAATTATGATTTTGAATGGCAACTGAGTTTGCAATTACTGATGAAGAAACGGTGAGCCATCCTTACTGCACTCTGATTAGGAAACAG
GTCATACAGACCGGGGCTTTCAATAGACAGTAAGGTGCGTATTACATCATGGCGCAGGCCATTTAGTGGCCAGAGATGACGTCGGATATATGTTAGCGAC
TGTCCCCTTTAATTGGCTATAGCATCATGAGGTGCGTCCATATAT
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1030 bp | 343 aa | 79 | 1108 | + | No |
Chemistry : DEDD
ORF sequence :
SIKVVGIDLAKNSFQVCVLSISGSILPNRKVNRGSLLDTIRQLPENTTIAMELCATSHHRGRIFQXQGFKVALIPAQHVKPFVGRXKNDANDARAICEAF
SRPDIHFVPVKSVEQQDLKSLRGVRARLVEQRTALANQIRGLSAEYGIIFPLSMKALNSYLPLIIEDAENELSHVMRGGLHTLYSDLIGIGERIDELTCK
ITMLGQQHPRYDAIKSIPSFAPILTAALLSEVGTGNQFQNGRQFSAWCGLVPKQHSSAGKSSLGSLSKSGNRDLRTLLIHGARAVMRYGINRTDAMGDWL
RSIIGRRGRAKAIVAMANKLGRIAWRVLATDSSCDIHKAFKAV
SRPDIHFVPVKSVEQQDLKSLRGVRARLVEQRTALANQIRGLSAEYGIIFPLSMKALNSYLPLIIEDAENELSHVMRGGLHTLYSDLIGIGERIDELTCK
ITMLGQQHPRYDAIKSIPSFAPILTAALLSEVGTGNQFQNGRQFSAWCGLVPKQHSSAGKSSLGSLSKSGNRDLRTLLIHGARAVMRYGINRTDAMGDWL
RSIIGRRGRAKAIVAMANKLGRIAWRVLATDSSCDIHKAFKAV
Blast result :
Comments
There is one copy of this IS element on the plasmid. The transposase encoded within it is degenerate. The inverted repeats are not at the termini of the IS but at 6 nt on the left end and at 4 nt on the right end. ISSod21 has 41% aa similarity to ISSod14.
References
1] Margie Romine (2007) Direct submission.