ISMrsp1
- Family IS1595
- Group ISPna2
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NZ_AAXY01000003.1 | ND | Marinobacter sp. | Marinobacter sp. ELB17 |
DNA section
IS Length : 1035 bp
Ends
IR Length : 28/31
IRL : GGCGCTGTTGTGCTTACCTGTTGGTGGTCTACACTTACTCCATACCTGAA
IRR : GGCGCTGTTGTGCTTACATGTTGGTAGGCTATTCCAAACTGGGAATCGCC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GCATATACTGA | TGTTATAT | ACAGTACAGGAGGC | 8 |
DNA sequence
GGCGCTGTTGTGCTTACCTGTTGGTGGTCTACACTTACTCCATACCTGAATGTATTTGGAGGCTACCGTGCAGCACGATGATTTCAACGACCTTCTACGC
CGCGTTCCTGACTTCACCACCCCACAGCTGAAAAGGCTACGACATCATATTGAAAAACGCGTCAAACTGGAGGCTGTTTACGAGACGCTGACGGCTAGTG
AAGAGGCGGTTACCCAGTGCCCTTATTGCCAGTCCAAGACGTTTATTCGCTGGGGATCATCCGAGAATGAGCGCCAGCGCTACCGTTGTAAACGTTGCGC
AAAGACCTTCAACGCGCTGGTGGGTAGCCCGCTGTACCGCATGCGTAAAGAAGAGCTCTGGCTAGAGTATGTGGAAACGATGCGCTATGGCCTATCCCTA
CGCAAAGCGGCCAAGGTGACCGGTGTCAGCCTACGCACGGCCTTTCGTTGGCGTCATGCCTTTCTAAGCAGTCCCAGAGATCATAAGGCTACGTCGTTGT
CCGGCATCATCGAGGCCGACGAGACATTCCTGCCGGAGTCCTTCAAGGGAAAAAAGACGATGCCTCGGCCAGCCCGTAAACGTGGGGGGCGGTAAGGTGA
CTCTGGTGCCCATCGCATTGGCGCTGGATCGCTCCGGACACATTACCCACCATGTTCTAGAGCACGACACTAAGGAGGAAATTATCGCAGCCTTGGCCTC
AGTGATCCGCGAGAACTCCGTCTTGTGCACAGATGGCAACCTTTCCTATGTAGAAATGGTGAAGACGTTTGACCATCTTGAGCATAAGCGATTGATTGCG
AAGGATAACAGCCGGGTTGTTGAGGGTGTTTACCACATCCAGACCCTGAACAATTTTACCCAACGCTGGAAGGGTTGGCTTAAGCGCTTCCACGGTGTCG
GAACGGCCCACGTGGACAATTACCTGGCGTGGTTCCGCTTTATGGATGAAAGGGAGGAGTTTCAAGATGTTGCTTGGGTTCGGGGGGCGATTCCCAGTTT
GGAATAGCCTACCAACATGTAAGCACAACAGCGCC
CGCGTTCCTGACTTCACCACCCCACAGCTGAAAAGGCTACGACATCATATTGAAAAACGCGTCAAACTGGAGGCTGTTTACGAGACGCTGACGGCTAGTG
AAGAGGCGGTTACCCAGTGCCCTTATTGCCAGTCCAAGACGTTTATTCGCTGGGGATCATCCGAGAATGAGCGCCAGCGCTACCGTTGTAAACGTTGCGC
AAAGACCTTCAACGCGCTGGTGGGTAGCCCGCTGTACCGCATGCGTAAAGAAGAGCTCTGGCTAGAGTATGTGGAAACGATGCGCTATGGCCTATCCCTA
CGCAAAGCGGCCAAGGTGACCGGTGTCAGCCTACGCACGGCCTTTCGTTGGCGTCATGCCTTTCTAAGCAGTCCCAGAGATCATAAGGCTACGTCGTTGT
CCGGCATCATCGAGGCCGACGAGACATTCCTGCCGGAGTCCTTCAAGGGAAAAAAGACGATGCCTCGGCCAGCCCGTAAACGTGGGGGGCGGTAAGGTGA
CTCTGGTGCCCATCGCATTGGCGCTGGATCGCTCCGGACACATTACCCACCATGTTCTAGAGCACGACACTAAGGAGGAAATTATCGCAGCCTTGGCCTC
AGTGATCCGCGAGAACTCCGTCTTGTGCACAGATGGCAACCTTTCCTATGTAGAAATGGTGAAGACGTTTGACCATCTTGAGCATAAGCGATTGATTGCG
AAGGATAACAGCCGGGTTGTTGAGGGTGTTTACCACATCCAGACCCTGAACAATTTTACCCAACGCTGGAAGGGTTGGCTTAAGCGCTTCCACGGTGTCG
GAACGGCCCACGTGGACAATTACCTGGCGTGGTTCCGCTTTATGGATGAAAGGGAGGAGTTTCAAGATGTTGCTTGGGTTCGGGGGGCGATTCCCAGTTT
GGAATAGCCTACCAACATGTAAGCACAACAGCGCC
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 546 bp | 181 aa | 50 | 595 | + | No |
Description : First part of the transposase
ORF sequence :
MYLEATVQHDDFNDLLRRVPDFTTPQLKRLRHHIEKRVKLEAVYETLTASEEAVTQCPYCQSKTFIRWGSSENERQRYRCKRCAKTFNALVGSPLYRMRK
EELWLEYVETMRYGLSLRKAAKVTGVSLRTAFRWRHAFLSSPRDHKATSLSGIIEADETFLPESFKGKKTMPRPARKRGGR
EELWLEYVETMRYGLSLRKAAKVTGVSLRTAFRWRHAFLSSPRDHKATSLSGIIEADETFLPESFKGKKTMPRPARKRGGR
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 426 bp | 141 aa | 582 | 1007 | + | No |
Description : Second part of the transposase
ORF sequence :
MGGGKVTLVPIALALDRSGHITHHVLEHDTKEEIIAALASVIRENSVLCTDGNLSYVEMVKTFDHLEHKRLIAKDNSRVVEGVYHIQTLNNFTQRWKGWL
KRFHGVGTAHVDNYLAWFRFMDEREEFQDVAWVRGAIPSLE
KRFHGVGTAHVDNYLAWFRFMDEREEFQDVAWVRGAIPSLE
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 958 bp | 331 aa | 50 | 1007 | + | Yes |
Chemistry : DDE
ORF sequence :
MYLEATVQHDDFNDLLRRVPDFTTPQLKRLRHHIEKRVKLEAVYETLTASEEAVTQCPYCQSKTFIRWGSSENERQRYRCKRCAKTFNALVGSPLYRMRK
EELWLEYVETMRYGLSLRKAAKVTGVSLRTAFRWRHAFLSSPRDHKATSLSGIIEADETFLPESFKGKKTMPRPARKRGGRRRCLGQPVNVGGGKVTLVP
IALALDRSGHITHHVLEHDTKEEIIAALASVIRENSVLCTDGNLSYVEMVKTFDHLEHKRLIAKDNSRVVEGVYHIQTLNNFTQRWKGWLKRFHGVGTAH
VDNYLAWFRFMDEREEFQDVAWVRGAIPSLE
EELWLEYVETMRYGLSLRKAAKVTGVSLRTAFRWRHAFLSSPRDHKATSLSGIIEADETFLPESFKGKKTMPRPARKRGGRRRCLGQPVNVGGGKVTLVP
IALALDRSGHITHHVLEHDTKEEIIAALASVIRENSVLCTDGNLSYVEMVKTFDHLEHKRLIAKDNSRVVEGVYHIQTLNNFTQRWKGWLKRFHGVGTAH
VDNYLAWFRFMDEREEFQDVAWVRGAIPSLE
Blast result :
Comments
ISMrsp1 is 57% aa similar to ISNe7. The third ORF is a putative ORFAB reconstructed in silico by possible frameshift.
References
1] Ward,B., Ferriera,S., Johnson,J., Kravitz,S., Beeson,K., Sutton,G., Rogers,Y.-H., Friedman,R., Frazier,M. and Venter,J.C. (20006) Direct submission GenBank.