ISfinder Mirror Site

ISBxe4

Family IS481
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_007953	ND	Burkholderia xenovorans	Burkholderia xenovorans LB400

DNA section

IS Length : 1128 bp

Ends

IR Length : 19/24

IRL : TGTCTGGTTGCGGAACGTCATTGGGATCAGGTAGCATGGGCTGTAGCCGA
IRR : TGTCTGTCTGGTTGCGAGACCTCATTGGCGTAAACCAAGATTCGGTCTAC

Insertion site

Left flank	Direct repeat	Right flank	DR Length
ACACAGTAGT	ACTACT	GCCTTGCTGT	6

DNA sequence

TGTCTGGTTGCGGAACGTCATTGGGATCAGGTAGCATGGGCTGTAGCCGAGTACGCGTGGTGCTTTCAACCTGTGATTGATGAGGTGCGGGCATGATGCA
AATCCATCCCCAGGCTCGAACGACCCCTGTGGTACGAGCAGAAATCGCACGATCCACGGAACCAACGAGCGTCGTCGCCAGGCGCTACGGCATCAGCGCT
GAGACAGTGCGCAAATGGCGTAAGCGCGGTGAGCAGGCATGCCAGGACCGATCGAGCAGACCCAAACGCCTGCGATGGCGTGCTAGTGAGGAGGAACGCG
CCATCATCTGTGCGGTGCGGCGTGCTACGGGCTTCCCCCTTGACGACCTGGGCTTCGTGCTGGGCCATTTCCTGCCGCATCTCAATCGCGACAGCATCTA
CCGCGTGCTGAAATCTGAAGGCCTCAACCGGCGCCCTCCAAAGCCGACGTCACTTCCTGAGAAAGGTCAGGGGCGCTTCAAGGAATACGATCTCGGCTAC
GTCCATATCGACGTCAAACACTTGCCAAAGCTGCGCACCGCTGACGGCGAGGTGCGCAAGCGCTACCTCTATGTCGCCATCGATCGCTGCTCTCGCTTTG
TGCACCTCGCCGTTTACGACGCCGAAAATACAACCAATGCAATCGCCTTCCTGGCACAAGCGCGAAAGGCCTTTCCCTTCCGCATCACCCATGTGCTGAC
GGATCGCGGATCCTGTTTCACCGCTGATGACTTCGAGCGCGCCTGCGCGAAACTCAAAGTCACCCGTCGCGTAACCAAACCCTATACGCCACAAACTAAT
GGCATGGTCGAGCGCTTCAACGGCCGCGTCGCAAGCGAAGTCCTTGGCATCAACATTGCCGGCCATGCAGATCTTGAAGTCTTGTTGAAAAGCTTCAACC
GTGCCTACAATCGACGACGACAGCGAGTCCTTAAAGGAAGCTCACCCATCGACAAAGTCGACCAACGTCTCAAGCTGAACGGATGCCTTGCCAACCCGCT
CTACAAGCCCCCAGCAGACCAGGAATACCTTCTATCTAAAGTAGACCGAATCTTGGTTTACGCCAATGAGGTCTCGCAACCAGACAGACA

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
996 bp	331 aa	93	1088	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MMQIHPQARTTPVVRAEIARSTEPTSVVARRYGISAETVRKWRKRGEQACQDRSSRPKRLRWRASEEERAIICAVRRATGFPLDDLGFVLGHFLPHLNRD
SIYRVLKSEGLNRRPPKPTSLPEKGQGRFKEYDLGYVHIDVKHLPKLRTADGEVRKRYLYVAIDRCSRFVHLAVYDAENTTNAIAFLAQARKAFPFRITH
VLTDRGSCFTADDFERACAKLKVTRRVTKPYTPQTNGMVERFNGRVASEVLGINIAGHADLEVLLKSFNRAYNRRRQRVLKGSSPIDKVDQRLKLNGCLA
NPLYKPPADQEYLLSKVDRILVYANEVSQPD

Blast result :

Comments

ISBxe4 is 41% aa similar to IS5564. There is no stop codon.

References

1] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina del Rio,T., Hammon,N., Israni,S., Pitluck,S., Chain,P., Malfatti,S., Shin,M., Vergez,L., Schmutz,J., Larimer,F., Land,M., Kyrpides,N., Lykidis,A. and Richardson,P.(2006) Direct Submission GenBank.