ISfinder Mirror Site

ISBrsp4

Family IS481
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_009485	ND	Bradyrhizobium sp.	Bradyrhizobium sp.

DNA section

IS Length : 1268 bp

Ends

IR Length : 44/50

IRL : TGTTCAGCCCGGGGACATGGCTGACAGGTGTTCGGAGACATGGTTGACAC
IRR : TGTTCAGCCCGGGGACATAGCTTACACCTGTTCGGGGACATAGTTGACAC

Insertion site

Left flank	Direct repeat	Right flank	DR Length
GACTGGATCG	CTTCGCTTC	CGATGACGCC	9

DNA sequence

TGTTCAGCCCGGGGACATGGCTGACAGGTGTTCGGAGACATGGTTGACACCTCAAAATCGGCTCGATTCGTCAAGCAAAGGGGCGAGCGATGCCGTGGCG
AGAGGTGTCTGTCATGGACCAGCGACGAGAGTTTGTGATGCTTGCCAAGCAGGAGGGCGTGAACCGGCGGAAGCTGTGCCGTCAGTTCGGCATCAGCGCC
GAGACCGGCTACAAGTGGATCCAGCGGTATGACGCCGGCGATACGACGCTGGCGGACCGGTCGCGGCGGCCGCATCGGAGCCCGGAGCGCACGGCGGCTG
CGACTGAGCAGCAGATCGTCGCGTTGCGCGACGCTCACCCGACCTGGGGCGCGCGCAAGCTCGCACGTCGTCTCGCGCGCGACGGGCAAACTGTCCCGGC
GATATCCACCGTGCACACCATCCTGCGCCGCTACGATCGCGTGAGCGAGCCTGCAGGCACGCCGGGGCAGCCGTACAGGCGCTTCGAGAAGGATGCGCCC
AATCAGCTGTGGCAGATGGACTTCAAGGGGCACAGTGCGCTGGAGAACGGCGTGTCCTGTCACCCGCTGACTGCGCTGGACGACCACTCGCGCTTCTCCG
TGTGCCTGGCGGCGTGTGACAACGAGCGCGGCTCTACGGTCAAAGGACATCTGGAGACGACGTTCCGCCGCTATGGACTGCCGGATGCGATGTTCGTCGA
TAATGGTGGTCCCTGGGGCTTTACTCTCGAAGACCCCTGGACCGCGCTGACGGTCTGGCTTCTGAAGCTCGGCATTCGAACGATCCACAGCCGGCCGTAT
CATCCTCAGGGCCGCGGCAAGAACGAACGCTTCCACCGCAGCCTGAAGGCCGAAGTCTTTGCCTTCAAGCGCTACAGGGACCTTGACGACGTCCAGCGGG
CGCTCGACGAATGGCGGGCCGTCTACAATCTCGACCGGCCCCACGAGGCTCTCGACTTCGATGTGCCGGCGAGCCGCTACCGGCCGAGCCCGCGAACGAT
GCCGGATCGCCTTCCGACGGTGGAATACGACGAGGGTGAGATCGTCCGTTCGGTGTCGAGCACCAAGGCCTATGTCAGTTTCGAGGGACGGCTCTGGAAA
GTCCCGCAAGCCTTTCGCAGCGAGCGGCTCGCTATTCGCCCGCTCAGTCAGGACGGAAAATTCGGAATTTTCTTTGCCGCGCATCAGATCGCAGCGATCG
ATTTGCGCGACCCCAAAAGTGTCAACTATGTCCCCGAACAGGTGTAAGCTATGTCCCCGGGCTGAACA

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
1110 bp	369 aa	138	1247	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MLAKQEGVNRRKLCRQFGISAETGYKWIQRYDAGDTTLADRSRRPHRSPERTAAATEQQIVALRDAHPTWGARKLARRLARDGQTVPAISTVHTILRRYD
RVSEPAGTPGQPYRRFEKDAPNQLWQMDFKGHSALENGVSCHPLTALDDHSRFSVCLAACDNERGSTVKGHLETTFRRYGLPDAMFVDNGGPWGFTLEDP
WTALTVWLLKLGIRTIHSRPYHPQGRGKNERFHRSLKAEVFAFKRYRDLDDVQRALDEWRAVYNLDRPHEALDFDVPASRYRPSPRTMPDRLPTVEYDEG
EIVRSVSSTKAYVSFEGRLWKVPQAFRSERLAIRPLSQDGKFGIFFAAHQIAAIDLRDPKSVNYVPEQV

Blast result :

Comments

ISBrsp4 is 52% aa similar to ISMyma5.

References

1] Giraud,E., Moulin,L., Vallenet,D., Barbe,V., Cytrin,E., Avarre,J.-C., Jaubert,M., Simon,D., Cartieaux,F., Prin,Y., Bena,G., Hannibal,L., Fardoux,J., Kojadinovic,M., Vuillet,L., Lajus,A., Cruveiller,S., Rouy,Z., Mangenot,S., Segurens,B., Dossat,C., Franck,W.L., Chang,W.-S., Saunders,E., Bruce,D., Richardson,P., Normand,P., Dreyfus,B., Pignol,D., Stacey,G., Emerich,D., Vermeglio,A., Medigue,C. and Sadowsky,M.(2007) Direct Submission GenBank.